MD04G1030100.v1.1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011630
Physical Location & Seq
Forward (+)
3405302 .. 3408849
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1030100.v1.1.491

Sequence Viewer

Length: 1704 bp
ATGTTCTTGATCATACAAATCTTGCTGCCATCAGCAGAAGCTAATCAGGCTTCTTCACCATCCCTTTTCTCCAAATTCTCCAGAGCAGGACCATCCAAAGAAGCAGCCAACAATATTGTATCTTTGTGCAAACATACCCCTCACCAGGCAGCTTGTGAATCCATTTTCATCTCCAAAACAAGTAATATTACTACAATTACTAAACCACCCCCTCAAACCCTTGAAGGCCTCCTTGTCCACTCAGTCGACTTCAGCATTATAAAGGCTCGTTTGGCTCGCGCTCTTGCCTATAATCTTACCATTTCCCATCGTCAAACCTCTCATCAAACACACTCGCTTGGTGGCATCAATGACTGCCTCGAGCTGCTCGAGGACAGTATCGATTTACTTGATAATGTCATACTCAAATACGACAGTACCAAAAGCACCTATGGCCATGCTGACGACGATATCCAAAGCTGGCTCAGTGCTGCTCTTACCAATCAAGAAACTTGTCTAGACAGCCTTGAGAGTGATACGTCAATGGTAGACAAGGGCGCTATGGAGACTTCAGCTAAAAATATGAACCAATTCATAAGTAACTCTCTGTCACTTTTCATGCTCACTACTAGGTCACCAAAGCAGGCCCAAAACAAAAACCCTAATAACATTGTCGGTGGCCGGAGGCTGTTGTCGGATGATTTTCCGAGTTGGGTGTCCGGCGCTGAGAGAAAGCTTCTAGAAGCTTCTGTGGGTGACTTAGAAGCACATGCTGTGGTGGCTTTGGATGGGAGTGGTACGCACAAGAGTATCGGTGAAGCACTTGGGTTGGTGGCCTCATTGGCTGAGAGTGGAGGAGGTGGCAGGTCTGTAATTCATGTAAAAGCTGGGACTTATCATGAATATATTAAAATACCAACGAAGCAGAAGAACGTTATGTTAATTGGTGATGGTTTGGGGACGACGATTGTTGTGGGCGATAGGAACTCTGATGATGGTTGGACCACTTTCCAGTCTGCCACGGTTGCTGCTATGGGAGACGGCTTTATAGCTCGTGACATAACGTTTGAGAATAATGCCGGTCCATCAAAGCACCAAGCGGTTGCCCTCCGAGTCGGCGCTGACAAGTCAGTGATTTTCCGATGCTCCATCGTCGGTTACCAAGACACCCTTTACACTCACTCCAAGCGCCAGTTCTACAGAGACACCCAAATCTACGGAACAGTTGATTTCATATTTGGAAACTCAGCAGCAGTTTTCCAGAACTGCAACATTTACGCAAGAAAACCCGCTTCAACCGGTCTCAAAAATTTTGTCACAGCACAAGGTAGAACCAGCCCGGATCAGAACACTGGAATTTCGATTCATAACTGCAAAATTTCAGCTGCTTCGGATCTTGCTCCGGTGAAATCCAAGTACGAGACGCATCTTGGAAGGCCATGGAAGCAGTACTCAAGAACAGTTATCATGCAGTCTTACTTGGATGACTCAATTAGTAAGGCTGGTTGGTCACCTTGGTCAGGTGGGTTTGCTTTAAACACATTGTATTATGGTGAGTATTCAAATTATGGGCCTGGGGCTTCAACTTCGGGTCGGGTCCGGTGGCCGGGATATCATGCATCACTTACACCGGTTGCTGCACAAGGCTTCACTGTTAGCGGTTTCATCTCTGGGAACCTTTGGTTGCCTTCCACTGGGGTTTCTTTTGATTCGGGACTTCATTAA

Protein Analysis

568

Amino Acids

61.17

Weight (kDa)

7.27

Isoelectric Point (pI)

32.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 38 - 198 1.3e-25 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 251 - 552 2.3e-136 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 834
AccI GTMKAC 2 cut(s) 246, 528
AccII CGCG 1 cut(s) 279
AciI CCGC 3 cut(s) 1079, 1269, 1638
AclI AACGTT 2 cut(s) 912, 1043
AclWI GGATC 2 cut(s) 1329, 1380
AcoI YGGCCR 3 cut(s) 433, 658, 1583
AcsI RAATTY 4 cut(s) 74, 1288, 1335, 1356
AcuI CTGAAG 2 cut(s) 235, 534
AfaI GTAC 4 cut(s) 418, 778, 1397, 1430
AfiI CCNNNNNNNGG 4 cut(s) 145, 1499, 1585, 1673
AgeI ACCGGT 2 cut(s) 1277, 1609
AgsI TTSAA 4 cut(s) 224, 1275, 1542, 1563
AjnI CCWGG 2 cut(s) 144, 1552
AjuI GAANNNNNNNTTGG 2 cut(s) 1157, 1189
Alw26I GTCTC 5 cut(s) 539, 1011, 1176, 1286, 1394
AlwI GGATC 2 cut(s) 1329, 1380
Ama87I CYCGRG 2 cut(s) 359, 368
AoxI GGCC 8 cut(s) 226, 433, 624, 658, 813, 1415, 1550, 1583
ApeKI GCWGC 9 cut(s) 25, 104, 149, 364, 470, 1007, 1229, 1364, 1616
ApoI RAATTY 4 cut(s) 74, 1288, 1335, 1356
ArsI GACNNNNNNTTYG 4 cut(s) 1028, 1060, 1278, 1310
AsiGI ACCGGT 2 cut(s) 1277, 1609
Asp700I GAANNNNTTC 1 cut(s) 569
AspLEI GCGC 5 cut(s) 281, 539, 704, 1100, 1170
AspS9I GGNCC 6 cut(s) 89, 625, 981, 1061, 1550, 1576
AsuC2I CCSGG 2 cut(s) 1319, 1587
AsuHPI GGTGA 9 cut(s) 48, 134, 606, 746, 806, 938, 1396, 1482, 1544
AvaI CYCGRG 2 cut(s) 359, 368
AvaII GGWCC 4 cut(s) 89, 981, 1061, 1576
BaeI ACNNNNGTAYC 4 cut(s) 400, 433, 772, 805
BalI TGGCCA 1 cut(s) 435
BauI CACGAG 1 cut(s) 1032
BbvI GCAGC 9 cut(s) 12, 116, 161, 351, 457, 994, 1241, 1351, 1603
BccI CCATC 9 cut(s) 37, 67, 100, 315, 761, 923, 968, 1072, 1136
BceAI ACGGC 1 cut(s) 1036
BciT130I CCWGG 2 cut(s) 146, 1554
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 2 cut(s) 1319, 1587
BcoDI GTCTC 5 cut(s) 539, 1011, 1176, 1286, 1394
BfaI CTAG 3 cut(s) 497, 609, 719
BfmI CTRYAG 1 cut(s) 1177
BfoI RGCGCY 4 cut(s) 540, 705, 1101, 1171
BfuAI ACCTGC 1 cut(s) 834
BglI GCCNNNNNGGC 1 cut(s) 821
BisI GCNGC 9 cut(s) 26, 105, 150, 365, 471, 1008, 1230, 1365, 1617
BlsI GCNGC 9 cut(s) 27, 106, 151, 366, 472, 1009, 1231, 1366, 1618
BmcAI AGTACT 1 cut(s) 1430
Bme1390I CCNGG 4 cut(s) 146, 1319, 1554, 1587
Bme18I GGWCC 4 cut(s) 89, 981, 1061, 1576
BmeT110I CYCGRG 2 cut(s) 359, 368
BmgT120I GGNCC 6 cut(s) 89, 625, 981, 1061, 1550, 1576
BmiI GGNNCC 2 cut(s) 1577, 1655
BmrFI CCNGG 4 cut(s) 146, 1319, 1554, 1587
BmrI ACTGGG 1 cut(s) 1683
BmsI GCATC 4 cut(s) 354, 1112, 1414, 1607
BmuI ACTGGG 1 cut(s) 1683
BpmI CTGGAG 1 cut(s) 64
BpuEI CTTGAG 2 cut(s) 527, 1417
BpuMI CCSGG 2 cut(s) 1319, 1587
Bsa29I ATCGAT 1 cut(s) 381
BsaI GGTCTC 1 cut(s) 1286
BsaJI CCNNGG 4 cut(s) 999, 1418, 1493, 1553
BsaWI WCCGGW 4 cut(s) 1277, 1381, 1578, 1609
BsaXI ACNNNNNCTCC 2 cut(s) 1145, 1175
Bsc4I CCNNNNNNNGG 4 cut(s) 145, 1499, 1585, 1673
Bse118I RCCGGY 3 cut(s) 1058, 1277, 1609
Bse1I ACTGG 4 cut(s) 991, 1171, 1336, 1678
BseBI CCWGG 2 cut(s) 146, 1554
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 4 cut(s) 999, 1418, 1493, 1553
BseGI GGATG 5 cut(s) 59, 92, 682, 772, 1468
BseLI CCNNNNNNNGG 4 cut(s) 145, 1499, 1585, 1673
BseMII CTCAG 5 cut(s) 255, 478, 696, 816, 1239
BseNI ACTGG 4 cut(s) 991, 1171, 1336, 1678
BseRI GAGGAG 1 cut(s) 849
BseXI GCAGC 9 cut(s) 12, 116, 161, 351, 457, 994, 1241, 1351, 1603
BseYI CCCAGC 1 cut(s) 866
BsgI GTGCAG 1 cut(s) 1602
Bsh1236I CGCG 1 cut(s) 279
BshFI GGCC 8 cut(s) 228, 435, 626, 660, 815, 1417, 1552, 1585
BshTI ACCGGT 2 cut(s) 1277, 1609
BshVI ATCGAT 1 cut(s) 381
BsiHKCI CYCGRG 2 cut(s) 359, 368
BsiSI CCGG 9 cut(s) 661, 699, 1059, 1278, 1319, 1382, 1579, 1586, 1610
BslFI GGGAC 2 cut(s) 883, 952
BslI CCNNNNNNNGG 4 cut(s) 145, 1499, 1585, 1673
BsmAI GTCTC 5 cut(s) 539, 1011, 1176, 1286, 1394
BsmBI CGTCTC 2 cut(s) 1011, 1394
BsmFI GGGAC 2 cut(s) 883, 952
BsnI GGCC 8 cut(s) 228, 435, 626, 660, 815, 1417, 1552, 1585
Bso31I GGTCTC 1 cut(s) 1286
BsoBI CYCGRG 2 cut(s) 359, 368
Bsp143I GATC 3 cut(s) 9, 1321, 1372
Bsp19I CCATGG 1 cut(s) 1418
BspACI CCGC 3 cut(s) 1079, 1269, 1638
BspANI GGCC 8 cut(s) 228, 435, 626, 660, 815, 1417, 1552, 1585
BspCNI CTCAG 5 cut(s) 254, 477, 697, 817, 1238
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 1 cut(s) 279
BspHI TCATGA 1 cut(s) 877
BspLI GGNNCC 2 cut(s) 1577, 1655
BspMI ACCTGC 1 cut(s) 834
BspPI GGATC 2 cut(s) 1329, 1380
BspTNI GGTCTC 1 cut(s) 1286
BsrFI RCCGGY 3 cut(s) 1058, 1277, 1609
BsrI ACTGG 4 cut(s) 991, 1171, 1336, 1678
BssAI RCCGGY 3 cut(s) 1058, 1277, 1609
BssECI CCNNGG 4 cut(s) 999, 1418, 1493, 1553
BssMI GATC 3 cut(s) 9, 1321, 1372
BssSI CACGAG 1 cut(s) 1032
BssT1I CCWWGG 2 cut(s) 1418, 1493
Bst2BI CACGAG 1 cut(s) 1032
Bst2UI CCWGG 2 cut(s) 146, 1554
Bst4CI ACNGT 6 cut(s) 377, 416, 1003, 1204, 1441, 1633
BstC8I GCNNGC 3 cut(s) 277, 461, 624
BstDEI CTNAG 6 cut(s) 241, 464, 705, 739, 825, 1225
BstDSI CCRYGG 2 cut(s) 999, 1418
BstEII GGTNACC 3 cut(s) 612, 1136, 1488
BstENI CCTNNNNNAGG 1 cut(s) 1497
BstF5I GGATG 5 cut(s) 59, 92, 682, 772, 1468
BstFNI CGCG 1 cut(s) 279
BstH2I RGCGCY 4 cut(s) 540, 705, 1101, 1171
BstHHI GCGC 5 cut(s) 281, 539, 704, 1100, 1170
BstKTI GATC 3 cut(s) 12, 1324, 1375
BstMAI GTCTC 5 cut(s) 539, 1011, 1176, 1286, 1394
BstMBI GATC 3 cut(s) 9, 1321, 1372
BstMWI GCNNNNNNNGC 7 cut(s) 47, 272, 432, 758, 821, 1004, 1423
BstNI CCWGG 2 cut(s) 146, 1554
BstNSI RCATGY 1 cut(s) 752
BstPI GGTNACC 3 cut(s) 612, 1136, 1488
BstSCI CCNGG 4 cut(s) 144, 1317, 1552, 1585
BstSFI CTRYAG 1 cut(s) 1177
BstUI CGCG 1 cut(s) 279
BstV1I GCAGC 9 cut(s) 12, 116, 161, 351, 457, 994, 1241, 1351, 1603
BstX2I RGATCY 1 cut(s) 1372
BstYI RGATCY 1 cut(s) 1372
Bsu15I ATCGAT 1 cut(s) 381
BsuRI GGCC 8 cut(s) 228, 435, 626, 660, 815, 1417, 1552, 1585
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 2 cut(s) 999, 1418
BtsCI GGATG 5 cut(s) 59, 92, 682, 772, 1468
BtsIMutI CAGTG 5 cut(s) 472, 1116, 1329, 1629, 1671
BveI ACCTGC 1 cut(s) 834
Cac8I GCNNGC 3 cut(s) 277, 461, 624
CciI TCATGA 1 cut(s) 877
CfoI GCGC 5 cut(s) 281, 539, 704, 1100, 1170
Cfr10I RCCGGY 3 cut(s) 1058, 1277, 1609
Cfr13I GGNCC 6 cut(s) 89, 625, 981, 1061, 1550, 1576
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 1 cut(s) 1411
Csp6I GTAC 4 cut(s) 417, 777, 1396, 1429
CspAI ACCGGT 2 cut(s) 1277, 1609
CviAII CATG 8 cut(s) 437, 598, 749, 857, 878, 1419, 1447, 1595
CviQI GTAC 4 cut(s) 417, 777, 1396, 1429
DdeI CTNAG 6 cut(s) 241, 464, 705, 739, 825, 1225
DpnI GATC 3 cut(s) 11, 1323, 1374
DpnII GATC 3 cut(s) 9, 1321, 1372
DraI TTTAAA 1 cut(s) 1515
EaeI YGGCCR 3 cut(s) 433, 658, 1583
Eco130I CCWWGG 2 cut(s) 1418, 1493
Eco147I AGGCCT 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 1286
Eco32I GATATC 2 cut(s) 451, 1592
Eco47I GGWCC 4 cut(s) 89, 981, 1061, 1576
Eco57I CTGAAG 2 cut(s) 235, 534
Eco88I CYCGRG 2 cut(s) 359, 368
Eco91I GGTNACC 3 cut(s) 612, 1136, 1488
EcoNI CCTNNNNNAGG 1 cut(s) 1497
EcoO65I GGTNACC 3 cut(s) 612, 1136, 1488
EcoRII CCWGG 2 cut(s) 144, 1552
EcoRV GATATC 2 cut(s) 451, 1592
EcoT14I CCWWGG 2 cut(s) 1418, 1493
EcoT22I ATGCAT 1 cut(s) 1600
ErhI CCWWGG 2 cut(s) 1418, 1493
Esp3I CGTCTC 2 cut(s) 1011, 1394
FaeI CATG 8 cut(s) 440, 601, 752, 860, 881, 1422, 1450, 1598
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 1134, 1166
FaqI GGGAC 2 cut(s) 883, 952
FatI CATG 8 cut(s) 436, 597, 748, 856, 877, 1418, 1446, 1594
FauI CCCGC 1 cut(s) 1276
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 2 cut(s) 246, 528
Fnu4HI GCNGC 9 cut(s) 26, 105, 150, 365, 471, 1008, 1230, 1365, 1617
FokI GGATG 5 cut(s) 46, 79, 689, 779, 1475
Fsp4HI GCNGC 9 cut(s) 26, 105, 150, 365, 471, 1008, 1230, 1365, 1617
FspBI CTAG 3 cut(s) 497, 609, 719
GlaI GCGC 5 cut(s) 280, 538, 703, 1099, 1169
GluI GCNGC 9 cut(s) 26, 105, 150, 365, 471, 1008, 1230, 1365, 1617
GsaI CCCAGC 1 cut(s) 870
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 4 cut(s) 540, 705, 1101, 1171
HaeIII GGCC 8 cut(s) 228, 435, 626, 660, 815, 1417, 1552, 1585
HapII CCGG 9 cut(s) 661, 699, 1059, 1278, 1319, 1382, 1579, 1586, 1610
HgaI GACGC 1 cut(s) 1411
HhaI GCGC 5 cut(s) 281, 539, 704, 1100, 1170
Hin1II CATG 8 cut(s) 440, 601, 752, 860, 881, 1422, 1450, 1598
Hin6I GCGC 5 cut(s) 279, 537, 702, 1098, 1168
HinP1I GCGC 5 cut(s) 279, 537, 702, 1098, 1168
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 2 cut(s) 713, 723
HinfI GANTC 5 cut(s) 158, 1092, 1342, 1466, 1688
HpaII CCGG 9 cut(s) 661, 699, 1059, 1278, 1319, 1382, 1579, 1586, 1610
HphI GGTGA 9 cut(s) 48, 134, 606, 746, 806, 938, 1396, 1482, 1544
Hpy166II GTNNAC 3 cut(s) 238, 247, 529
Hpy188I TCNGA 7 cut(s) 676, 687, 970, 1091, 1121, 1326, 1372
Hpy8I GTNNAC 3 cut(s) 238, 247, 529
Hpy99I CGWCG 3 cut(s) 449, 946, 1136
HpyAV CCTTC 3 cut(s) 218, 1407, 1677
HpyCH4III ACNGT 6 cut(s) 377, 416, 1003, 1204, 1441, 1633
HpyCH4IV ACGT 3 cut(s) 518, 912, 1043
HpyCH4V TGCA 6 cut(s) 129, 1248, 1353, 1450, 1598, 1619
HpyF10VI GCNNNNNNNGC 7 cut(s) 47, 272, 432, 758, 821, 1004, 1423
HpyF3I CTNAG 6 cut(s) 241, 464, 705, 739, 825, 1225
HpySE526I ACGT 3 cut(s) 518, 912, 1043
Hsp92II CATG 8 cut(s) 440, 601, 752, 860, 881, 1422, 1450, 1598
HspAI GCGC 5 cut(s) 279, 537, 702, 1098, 1168
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 3 cut(s) 9, 1321, 1372
LmnI GCTCC 2 cut(s) 1130, 1384
Lsp1109I GCAGC 9 cut(s) 12, 116, 161, 351, 457, 994, 1241, 1351, 1603
LweI GCATC 4 cut(s) 354, 1112, 1414, 1607
MaeI CTAG 3 cut(s) 497, 609, 719
MaeII ACGT 3 cut(s) 518, 912, 1043
MaeIII GTNAC 8 cut(s) 578, 588, 612, 734, 1034, 1136, 1294, 1488
MalI GATC 3 cut(s) 11, 1323, 1374
MboI GATC 3 cut(s) 9, 1321, 1372
MboII GAAGA 2 cut(s) 45, 919
MflI RGATCY 1 cut(s) 1372
MlsI TGGCCA 1 cut(s) 435
MluNI TGGCCA 1 cut(s) 435
MlyI GAGTC 2 cut(s) 1101, 1460
MmeI TCCRAC 2 cut(s) 654, 959
Mox20I TGGCCA 1 cut(s) 435
Mph1103I ATGCAT 1 cut(s) 1600
MroXI GAANNNNTTC 1 cut(s) 569
MscI TGGCCA 1 cut(s) 435
MseI TTAA 4 cut(s) 888, 920, 1514, 1702
Msp20I TGGCCA 1 cut(s) 435
MspA1I CMGCKG 1 cut(s) 1364
MspI CCGG 9 cut(s) 661, 699, 1059, 1278, 1319, 1382, 1579, 1586, 1610
MspR9I CCNGG 4 cut(s) 146, 1319, 1554, 1587
MvaI CCWGG 2 cut(s) 146, 1554
MvnI CGCG 1 cut(s) 279
MwoI GCNNNNNNNGC 7 cut(s) 47, 272, 432, 758, 821, 1004, 1423
NciI CCSGG 2 cut(s) 1319, 1587
NcoI CCATGG 1 cut(s) 1418
NdeII GATC 3 cut(s) 9, 1321, 1372
NlaIII CATG 8 cut(s) 440, 601, 752, 860, 881, 1422, 1450, 1598
NlaIV GGNNCC 2 cut(s) 1577, 1655
NmuCI GTSAC 6 cut(s) 588, 612, 734, 1034, 1294, 1488
NsiI ATGCAT 1 cut(s) 1600
NspI RCATGY 1 cut(s) 752
PaeR7I CTCGAG 2 cut(s) 359, 368
PagI TCATGA 1 cut(s) 877
PceI AGGCCT 1 cut(s) 228
PcsI WCGNNNNNNNCGW 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 569
PfeI GAWTC 3 cut(s) 158, 1342, 1688
PinAI ACCGGT 2 cut(s) 1277, 1609
PkrI GCNGC 9 cut(s) 27, 106, 151, 366, 472, 1009, 1231, 1366, 1618
PleI GAGTC 2 cut(s) 1100, 1460
PpsI GAGTC 2 cut(s) 1100, 1460
PsiI TTATAA 1 cut(s) 260
Psp1406I AACGTT 2 cut(s) 912, 1043
Psp6I CCWGG 2 cut(s) 144, 1552
PspEI GGTNACC 3 cut(s) 612, 1136, 1488
PspFI CCCAGC 1 cut(s) 866
PspGI CCWGG 2 cut(s) 144, 1552
PspN4I GGNNCC 2 cut(s) 1577, 1655
PspPI GGNCC 6 cut(s) 89, 625, 981, 1061, 1550, 1576
PspXI VCTCGAGB 2 cut(s) 359, 368
PsuI RGATCY 1 cut(s) 1372
PvuII CAGCTG 1 cut(s) 1364
RsaI GTAC 4 cut(s) 418, 778, 1397, 1430
RsaNI GTAC 4 cut(s) 417, 777, 1396, 1429
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 4 cut(s) 888, 920, 1514, 1702
SatI GCNGC 9 cut(s) 26, 105, 150, 365, 471, 1008, 1230, 1365, 1617
Sau3AI GATC 3 cut(s) 9, 1321, 1372
Sau96I GGNCC 6 cut(s) 89, 625, 981, 1061, 1550, 1576
ScaI AGTACT 1 cut(s) 1430
SchI GAGTC 2 cut(s) 1101, 1460
ScrFI CCNGG 4 cut(s) 146, 1319, 1554, 1587
SfaNI GCATC 4 cut(s) 354, 1112, 1414, 1607
SfcI CTRYAG 1 cut(s) 1177
Sfr274I CTCGAG 2 cut(s) 359, 368
SinI GGWCC 4 cut(s) 89, 981, 1061, 1576
SlaI CTCGAG 2 cut(s) 359, 368
SmlI CTYRAG 4 cut(s) 359, 368, 506, 1432
SmoI CTYRAG 4 cut(s) 359, 368, 506, 1432
SseBI AGGCCT 1 cut(s) 228
SsiI CCGC 3 cut(s) 1079, 1269, 1638
SspI AATATT 2 cut(s) 115, 187
SspMI CTAG 3 cut(s) 497, 609, 719
StuI AGGCCT 1 cut(s) 228
StyD4I CCNGG 4 cut(s) 144, 1317, 1552, 1585
StyI CCWWGG 2 cut(s) 1418, 1493
TaaI ACNGT 6 cut(s) 377, 416, 1003, 1204, 1441, 1633
TaiI ACGT 3 cut(s) 521, 915, 1046
TaqI TCGA 5 cut(s) 246, 360, 369, 381, 1340
TatI WGTACW 1 cut(s) 1428
TfiI GAWTC 3 cut(s) 158, 1342, 1688
Tru1I TTAA 4 cut(s) 888, 920, 1514, 1702
Tru9I TTAA 4 cut(s) 888, 920, 1514, 1702
TscAI CASTG 5 cut(s) 472, 1116, 1336, 1636, 1678
TseFI GTSAC 6 cut(s) 588, 612, 734, 1034, 1294, 1488
TseI GCWGC 9 cut(s) 25, 104, 149, 364, 470, 1007, 1229, 1364, 1616
Tsp45I GTSAC 6 cut(s) 588, 612, 734, 1034, 1294, 1488
TspGWI ACGGA 1 cut(s) 1212
TspRI CASTG 5 cut(s) 472, 1116, 1336, 1636, 1678
VpaK11BI GGWCC 4 cut(s) 89, 981, 1061, 1576
XagI CCTNNNNNAGG 1 cut(s) 1497
XapI RAATTY 4 cut(s) 74, 1288, 1335, 1356
XbaI TCTAGA 2 cut(s) 496, 718
XceI RCATGY 1 cut(s) 752
XhoI CTCGAG 2 cut(s) 359, 368
XmiI GTMKAC 2 cut(s) 246, 528
XmnI GAANNNNTTC 1 cut(s) 569
XspI CTAG 3 cut(s) 497, 609, 719
ZrmI AGTACT 1 cut(s) 1430
Zsp2I ATGCAT 1 cut(s) 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.