Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011630

Physical Location & Seq

Genomic Coordinates Forward (+)

3286206 .. 3290705

Gene Structure 4500 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1028900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGTTTACAATACTGAGTTCCTCCAATGGAGAAGATAGAAGCAAAACTCACACTTTCTTTCTAACCATGTCCTTGATCATAGAAATCTCACTACTATCAGCAGAAGCTAATCAGGCTTCTTCACCATCCCATTTCTCCAAATTCTCCAGAGTAGGACCATCCAAAGTAGCCAACAATATTGTATCTTTTTGCAAACATACCCCTCACCAAGCAGCTTGTGAATCCATTTTCATCTCCAAAACAAGTAATAGTACTACAATTACTAAACCACCCCCTCAAACCCTTGAAGGCCTCTTTGTCCACTCAGTCGACTTCAGCATTATAAAGGCTCGTTTGGCTTCCGCTCTTACCTATAATCTTACCATTTCCCATCGTCAAACCTCTCATCAAACACACTCGCTTGGAGGCATCAACGACTGCCTCGAGCTGCTCGAGGACAGTATCGATTTACTCGATAATGTCATACTCAAATACGACAGTACCAAAAGCGCCTATGGCCACACTGACGACGATATCCAAAGTTGGCTCAGTGCTGCTCTTACCAATCAAGAAACTTGTCTAGACAGCCTTGAGAGTGATAAGTCTGTGGTAGACAAGGGCGCTATGGAGACTTCAGCTCAAAATATGAACCAATTCATAAGTAACTCCCTGTCACTTTTCATGCTCACTAGTAGGTCACCAAAGCAGGCCCAAAACAAAAACCCTAGTAACATTGTCGGTGGCCGGAGGCTGTTGTCGGATGATTTTCCGAGTTGGGTGTCCGGCGCTGAGAGAAAGCTTCTAGAAGCTTCTGTGGGTGACTTAGAAGCACATACTGTGGTGGCTTTGGATGGGAGTGGTACGCACAAGAGTATCGGTGAAGCACTTGGGTTGGTGGCCTCATTGGCTGAGAGTGGAGGAGGTGGCAGATCTGTAATTCATGTAAAAGCTGGGACTTATCATGAATATATTAAAATACCAACGAAGCAGAAGAACGTTATGTTAATTGGTGATGGTTTGGGGACGACGATTATTGTCGGCGATAGGAACTCTGATGATGGTTGGACCACTTTCCAGTCTGCCACAGTTGCTGCTATGGGAGACGGATTTATAGCTCGTGACATAACATTTATGAACAGCGCCGATCCATCAAAGCACCAAGCAGTTGCCCTTCTGGTCGGCGCGGATAAGTCAGTGATTTTTAGATGCTCCGTCATTGGCTACCAAGACACCCTCTACACTCATTCCAAGCGCCAGTTCTACAGAGACACCCAAATCTACGGAACAGTTGATTTCATATTTGGAAACTCAGCAGCAGTTTTCCAAAATTGCAACATTTACGCAAGAAAACCCTTTTCGACCGGTCTCAAAAACTTTGTCACAGCACAAGGCAGAACTAGCCCGGATCAGAACACTGGAATTTCGATTCACAACTGCAAAATTTCGGCTGCTTCGGACCTTGCTCCGGTGAAATCCAAGTACGAGACGTATCTTGGGAGGCCATGGATGCAGTATTCAAGAACAGTTATCATGCAGTCTTACTTGGATGACTCAATCAGTAAGGCTGGTTGGTCACCTTGGTCAGGTGGGTTTGCTTTAAATACATTGTATTATGGTGAGTATTCAAATTATGGGCCTGGGGCTTCAACTTCGGGTCGGGTCCAGTGGCCAGGGTATCATGCATCACTTACACCGGTTGCTGCACAAGCCTTCACTGTTAGTGGTTTCATCTCTGGGAACCTTTGGTTGCCTTCCACTGGGGTTTCTTTTGATCCGGGACTCATTAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

590

Amino Acids

63.75

Weight (kDa)

6.63

Isoelectric Point (pI)

31.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	59 - 219	6e-26	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	272 - 573	8.3e-134	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43050 AT3G59010
fragaria_vesca	FvH4_4g12140
malus_domestica	MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica	Prupe.1G178400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0409791
rosa_laevigata	RLG00000008522
rosa_multiflora	Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354340
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana	Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	323
AccBSI	CCGCTC	1 cut(s)	344
AccI	GTMKAC	2 cut(s)	309, 591
AccII	CGCG	1 cut(s)	1163
AciI	CCGC	2 cut(s)	342, 1163
AclI	AACGTT	1 cut(s)	975
AclWI	GGATC	3 cut(s)	1118, 1392, 1745
AcoI	YGGCCR	3 cut(s)	496, 721, 1646
AcsI	RAATTY	3 cut(s)	140, 1398, 1419
AcuI	CTGAAG	2 cut(s)	298, 597
AfaI	GTAC	4 cut(s)	253, 481, 841, 1460
AfiI	CCNNNNNNNGG	3 cut(s)	1444, 1562, 1736
AgeI	ACCGGT	2 cut(s)	1340, 1672
AgsI	TTSAA	4 cut(s)	287, 1497, 1605, 1626
AhlI	ACTAGT	1 cut(s)	668
AjnI	CCWGG	2 cut(s)	1615, 1648
AjuI	GAANNNNNNNTTGG	2 cut(s)	1220, 1252
AluBI	AGCT	8 cut(s)	107, 215, 427, 617, 778, 788, 929, 1094
AluI	AGCT	8 cut(s)	107, 215, 427, 617, 778, 788, 929, 1094
Alw26I	GTCTC	5 cut(s)	602, 1074, 1239, 1349, 1457
AlwI	GGATC	3 cut(s)	1118, 1392, 1745
AlwNI	CAGNNNCTG	1 cut(s)	1070
Ama87I	CYCGRG	2 cut(s)	422, 431
AoxI	GGCC	8 cut(s)	289, 496, 687, 721, 876, 1478, 1613, 1646
ApeKI	GCWGC	7 cut(s)	212, 427, 533, 1070, 1292, 1427, 1679
ApoI	RAATTY	3 cut(s)	140, 1398, 1419
ArsI	GACNNNNNNTTYG	2 cut(s)	1341, 1373
AsiGI	ACCGGT	2 cut(s)	1340, 1672
Asp700I	GAANNNNTTC	1 cut(s)	632
AspLEI	GCGC	6 cut(s)	491, 602, 767, 1121, 1163, 1233
AspS9I	GGNCC	6 cut(s)	155, 688, 1044, 1435, 1613, 1639
AsuC2I	CCSGG	2 cut(s)	1382, 1755
AsuHPI	GGTGA	9 cut(s)	114, 197, 669, 809, 869, 1001, 1459, 1545, 1607
AvaI	CYCGRG	2 cut(s)	422, 431
AvaII	GGWCC	4 cut(s)	155, 1044, 1435, 1639
BaeI	ACNNNNGTAYC	4 cut(s)	463, 496, 835, 868
BalI	TGGCCA	2 cut(s)	498, 1648
BauI	CACGAG	1 cut(s)	1095
BbvI	GCAGC	7 cut(s)	224, 414, 520, 1057, 1304, 1414, 1666
BccI	CCATC	7 cut(s)	133, 166, 378, 824, 986, 1031, 1135
BciT130I	CCWGG	2 cut(s)	1617, 1650
BclI	TGATCA	1 cut(s)	75
BcnI	CCSGG	2 cut(s)	1382, 1755
BcoDI	GTCTC	5 cut(s)	602, 1074, 1239, 1349, 1457
BcuI	ACTAGT	1 cut(s)	668
BfaI	CTAG	5 cut(s)	560, 669, 705, 782, 1377
BfmI	CTRYAG	1 cut(s)	1240
BfoI	RGCGCY	5 cut(s)	492, 603, 768, 1122, 1234
BglI	GCCNNNNNGGC	1 cut(s)	884
BglII	AGATCT	1 cut(s)	908
BisI	GCNGC	7 cut(s)	213, 428, 534, 1071, 1293, 1428, 1680
BlsI	GCNGC	7 cut(s)	214, 429, 535, 1072, 1294, 1429, 1681
BmcAI	AGTACT	1 cut(s)	253
Bme1390I	CCNGG	4 cut(s)	1382, 1617, 1650, 1755
Bme18I	GGWCC	4 cut(s)	155, 1044, 1435, 1639
BmeT110I	CYCGRG	2 cut(s)	422, 431
BmgT120I	GGNCC	6 cut(s)	155, 688, 1044, 1435, 1613, 1639
BmiI	GGNNCC	2 cut(s)	1640, 1718
BmrFI	CCNGG	4 cut(s)	1382, 1617, 1650, 1755
BmrI	ACTGGG	1 cut(s)	1746
BmsI	GCATC	4 cut(s)	417, 1175, 1476, 1670
BmuI	ACTGGG	1 cut(s)	1746
BpmI	CTGGAG	1 cut(s)	130
BpuEI	CTTGAG	1 cut(s)	590
BpuMI	CCSGG	2 cut(s)	1382, 1755
Bsa29I	ATCGAT	1 cut(s)	444
BsaI	GGTCTC	1 cut(s)	1349
BsaJI	CCNNGG	4 cut(s)	1481, 1556, 1616, 1649
BsaWI	WCCGGW	3 cut(s)	1340, 1444, 1672
Bsc4I	CCNNNNNNNGG	3 cut(s)	1444, 1562, 1736
Bse118I	RCCGGY	2 cut(s)	1340, 1672
Bse1I	ACTGG	5 cut(s)	1054, 1234, 1399, 1642, 1741
BseBI	CCWGG	2 cut(s)	1617, 1650
BseCI	ATCGAT	1 cut(s)	444
BseDI	CCNNGG	4 cut(s)	1481, 1556, 1616, 1649
BseGI	GGATG	6 cut(s)	125, 158, 745, 835, 1491, 1531
BseLI	CCNNNNNNNGG	3 cut(s)	1444, 1562, 1736
BseMII	CTCAG	6 cut(s)	5, 318, 541, 759, 879, 1302
BseNI	ACTGG	5 cut(s)	1054, 1234, 1399, 1642, 1741
BseRI	GAGGAG	1 cut(s)	912
BseXI	GCAGC	7 cut(s)	224, 414, 520, 1057, 1304, 1414, 1666
BseYI	CCCAGC	1 cut(s)	929
BsgI	GTGCAG	1 cut(s)	1665
Bsh1236I	CGCG	1 cut(s)	1163
Bsh1285I	CGRYCG	1 cut(s)	1341
BshFI	GGCC	8 cut(s)	291, 498, 689, 723, 878, 1480, 1615, 1648
BshTI	ACCGGT	2 cut(s)	1340, 1672
BshVI	ATCGAT	1 cut(s)	444
BsiEI	CGRYCG	1 cut(s)	1341
BsiHKCI	CYCGRG	2 cut(s)	422, 431
BsiSI	CCGG	7 cut(s)	724, 762, 1341, 1382, 1445, 1673, 1754
BslFI	GGGAC	2 cut(s)	946, 1015
BslI	CCNNNNNNNGG	3 cut(s)	1444, 1562, 1736
BsmAI	GTCTC	5 cut(s)	602, 1074, 1239, 1349, 1457
BsmBI	CGTCTC	2 cut(s)	1074, 1457
BsmFI	GGGAC	2 cut(s)	946, 1015
BsnI	GGCC	8 cut(s)	291, 498, 689, 723, 878, 1480, 1615, 1648
Bso31I	GGTCTC	1 cut(s)	1349
BsoBI	CYCGRG	2 cut(s)	422, 431
Bsp143I	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
Bsp19I	CCATGG	1 cut(s)	1481
BspACI	CCGC	2 cut(s)	342, 1163
BspANI	GGCC	8 cut(s)	291, 498, 689, 723, 878, 1480, 1615, 1648
BspCNI	CTCAG	6 cut(s)	6, 317, 540, 760, 880, 1301
BspDI	ATCGAT	1 cut(s)	444
BspFNI	CGCG	1 cut(s)	1163
BspHI	TCATGA	1 cut(s)	940
BspLI	GGNNCC	2 cut(s)	1640, 1718
BspPI	GGATC	3 cut(s)	1118, 1392, 1745
BspTNI	GGTCTC	1 cut(s)	1349
BsrBI	CCGCTC	1 cut(s)	344
BsrFI	RCCGGY	2 cut(s)	1340, 1672
BsrI	ACTGG	5 cut(s)	1054, 1234, 1399, 1642, 1741
BssAI	RCCGGY	2 cut(s)	1340, 1672
BssECI	CCNNGG	4 cut(s)	1481, 1556, 1616, 1649
BssMI	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
BssSI	CACGAG	1 cut(s)	1095
BssT1I	CCWWGG	2 cut(s)	1481, 1556
Bst2BI	CACGAG	1 cut(s)	1095
Bst2UI	CCWGG	2 cut(s)	1617, 1650
Bst4CI	ACNGT	7 cut(s)	440, 479, 817, 1066, 1267, 1504, 1696
BstC8I	GCNNGC	1 cut(s)	687
BstDEI	CTNAG	7 cut(s)	14, 304, 527, 768, 802, 888, 1288
BstDSI	CCRYGG	1 cut(s)	1481
BstEII	GGTNACC	2 cut(s)	675, 1551
BstENI	CCTNNNNNAGG	1 cut(s)	1560
BstF5I	GGATG	6 cut(s)	125, 158, 745, 835, 1491, 1531
BstFNI	CGCG	1 cut(s)	1163
BstH2I	RGCGCY	5 cut(s)	492, 603, 768, 1122, 1234
BstHHI	GCGC	6 cut(s)	491, 602, 767, 1121, 1163, 1233
BstKTI	GATC	5 cut(s)	78, 911, 1126, 1387, 1753
BstMAI	GTCTC	5 cut(s)	602, 1074, 1239, 1349, 1457
BstMBI	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
BstMCI	CGRYCG	1 cut(s)	1341
BstMWI	GCNNNNNNNGC	8 cut(s)	113, 335, 495, 884, 1067, 1377, 1486, 1685
BstNI	CCWGG	2 cut(s)	1617, 1650
BstPI	GGTNACC	2 cut(s)	675, 1551
BstSCI	CCNGG	4 cut(s)	1380, 1615, 1648, 1753
BstSFI	CTRYAG	1 cut(s)	1240
BstUI	CGCG	1 cut(s)	1163
BstV1I	GCAGC	7 cut(s)	224, 414, 520, 1057, 1304, 1414, 1666
BstX2I	RGATCY	1 cut(s)	908
BstYI	RGATCY	1 cut(s)	908
Bsu15I	ATCGAT	1 cut(s)	444
BsuRI	GGCC	8 cut(s)	291, 498, 689, 723, 878, 1480, 1615, 1648
BsuTUI	ATCGAT	1 cut(s)	444
BtgI	CCRYGG	1 cut(s)	1481
BtsCI	GGATG	6 cut(s)	125, 158, 745, 835, 1491, 1531
BtsIMutI	CAGTG	7 cut(s)	501, 535, 1179, 1392, 1649, 1692, 1734
Cac8I	GCNNGC	1 cut(s)	687
CaiI	CAGNNNCTG	1 cut(s)	1070
CciI	TCATGA	1 cut(s)	940
CfoI	GCGC	6 cut(s)	491, 602, 767, 1121, 1163, 1233
Cfr10I	RCCGGY	2 cut(s)	1340, 1672
Cfr13I	GGNCC	6 cut(s)	155, 688, 1044, 1435, 1613, 1639
ClaI	ATCGAT	1 cut(s)	444
Csp6I	GTAC	4 cut(s)	252, 480, 840, 1459
CspAI	ACCGGT	2 cut(s)	1340, 1672
CviAII	CATG	7 cut(s)	67, 661, 920, 941, 1482, 1510, 1658
CviQI	GTAC	4 cut(s)	252, 480, 840, 1459
DdeI	CTNAG	7 cut(s)	14, 304, 527, 768, 802, 888, 1288
DpnI	GATC	5 cut(s)	77, 910, 1125, 1386, 1752
DpnII	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
DraI	TTTAAA	1 cut(s)	1578
EaeI	YGGCCR	3 cut(s)	496, 721, 1646
Eco130I	CCWWGG	2 cut(s)	1481, 1556
Eco147I	AGGCCT	1 cut(s)	291
Eco31I	GGTCTC	1 cut(s)	1349
Eco32I	GATATC	1 cut(s)	514
Eco47I	GGWCC	4 cut(s)	155, 1044, 1435, 1639
Eco57I	CTGAAG	2 cut(s)	298, 597
Eco88I	CYCGRG	2 cut(s)	422, 431
Eco91I	GGTNACC	2 cut(s)	675, 1551
EcoNI	CCTNNNNNAGG	1 cut(s)	1560
EcoO65I	GGTNACC	2 cut(s)	675, 1551
EcoRII	CCWGG	2 cut(s)	1615, 1648
EcoRV	GATATC	1 cut(s)	514
EcoT14I	CCWWGG	2 cut(s)	1481, 1556
EcoT22I	ATGCAT	1 cut(s)	1663
ErhI	CCWWGG	2 cut(s)	1481, 1556
Esp3I	CGTCTC	2 cut(s)	1074, 1457
FaeI	CATG	7 cut(s)	70, 664, 923, 944, 1485, 1513, 1661
FaqI	GGGAC	2 cut(s)	946, 1015
FatI	CATG	7 cut(s)	66, 660, 919, 940, 1481, 1509, 1657
FbaI	TGATCA	1 cut(s)	75
FblI	GTMKAC	2 cut(s)	309, 591
Fnu4HI	GCNGC	7 cut(s)	213, 428, 534, 1071, 1293, 1428, 1680
FokI	GGATG	6 cut(s)	112, 145, 752, 842, 1498, 1538
Fsp4HI	GCNGC	7 cut(s)	213, 428, 534, 1071, 1293, 1428, 1680
FspBI	CTAG	5 cut(s)	560, 669, 705, 782, 1377
GlaI	GCGC	6 cut(s)	490, 601, 766, 1120, 1162, 1232
GluI	GCNGC	7 cut(s)	213, 428, 534, 1071, 1293, 1428, 1680
GsaI	CCCAGC	1 cut(s)	933
GsuI	CTGGAG	1 cut(s)	130
HaeII	RGCGCY	5 cut(s)	492, 603, 768, 1122, 1234
HaeIII	GGCC	8 cut(s)	291, 498, 689, 723, 878, 1480, 1615, 1648
HapII	CCGG	7 cut(s)	724, 762, 1341, 1382, 1445, 1673, 1754
HhaI	GCGC	6 cut(s)	491, 602, 767, 1121, 1163, 1233
Hin1II	CATG	7 cut(s)	70, 664, 923, 944, 1485, 1513, 1661
Hin6I	GCGC	6 cut(s)	489, 600, 765, 1119, 1161, 1231
HinP1I	GCGC	6 cut(s)	489, 600, 765, 1119, 1161, 1231
HincII	GTYRAC	1 cut(s)	310
HindII	GTYRAC	1 cut(s)	310
HindIII	AAGCTT	2 cut(s)	776, 786
HinfI	GANTC	4 cut(s)	221, 1405, 1529, 1758
HpaII	CCGG	7 cut(s)	724, 762, 1341, 1382, 1445, 1673, 1754
HphI	GGTGA	9 cut(s)	114, 197, 669, 809, 869, 1001, 1459, 1545, 1607
Hpy166II	GTNNAC	4 cut(s)	6, 301, 310, 592
Hpy188I	TCNGA	5 cut(s)	739, 750, 1033, 1389, 1435
Hpy188III	TCNNGA	7 cut(s)	147, 548, 560, 782, 941, 1097, 1497
Hpy8I	GTNNAC	4 cut(s)	6, 301, 310, 592
Hpy99I	CGWCG	2 cut(s)	512, 1009
HpyAV	CCTTC	4 cut(s)	281, 1160, 1699, 1740
HpyCH4III	ACNGT	7 cut(s)	440, 479, 817, 1066, 1267, 1504, 1696
HpyCH4IV	ACGT	2 cut(s)	975, 1466
HpyCH4V	TGCA	7 cut(s)	192, 1311, 1416, 1489, 1513, 1661, 1682
HpyF10VI	GCNNNNNNNGC	8 cut(s)	113, 335, 495, 884, 1067, 1377, 1486, 1685
HpyF3I	CTNAG	7 cut(s)	14, 304, 527, 768, 802, 888, 1288
HpySE526I	ACGT	2 cut(s)	975, 1466
Hsp92II	CATG	7 cut(s)	70, 664, 923, 944, 1485, 1513, 1661
HspAI	GCGC	6 cut(s)	489, 600, 765, 1119, 1161, 1231
Ksp22I	TGATCA	1 cut(s)	75
Kzo9I	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
LmnI	GCTCC	2 cut(s)	1193, 1447
Lsp1109I	GCAGC	7 cut(s)	224, 414, 520, 1057, 1304, 1414, 1666
LweI	GCATC	4 cut(s)	417, 1175, 1476, 1670
MaeI	CTAG	5 cut(s)	560, 669, 705, 782, 1377
MaeII	ACGT	2 cut(s)	975, 1466
MaeIII	GTNAC	8 cut(s)	641, 651, 675, 707, 797, 1097, 1357, 1551
MalI	GATC	5 cut(s)	77, 910, 1125, 1386, 1752
MbiI	CCGCTC	1 cut(s)	344
MboI	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
MboII	GAAGA	3 cut(s)	44, 111, 982
MflI	RGATCY	1 cut(s)	908
MlsI	TGGCCA	2 cut(s)	498, 1648
MluCI	AATT	9 cut(s)	140, 258, 632, 915, 984, 1306, 1398, 1419, 1606
MluNI	TGGCCA	2 cut(s)	498, 1648
MlyI	GAGTC	2 cut(s)	1523, 1752
MmeI	TCCRAC	2 cut(s)	717, 1022
Mox20I	TGGCCA	2 cut(s)	498, 1648
Mph1103I	ATGCAT	1 cut(s)	1663
MroXI	GAANNNNTTC	1 cut(s)	632
MscI	TGGCCA	2 cut(s)	498, 1648
MseI	TTAA	4 cut(s)	951, 983, 1577, 1764
Msp20I	TGGCCA	2 cut(s)	498, 1648
MspI	CCGG	7 cut(s)	724, 762, 1341, 1382, 1445, 1673, 1754
MspR9I	CCNGG	4 cut(s)	1382, 1617, 1650, 1755
MvaI	CCWGG	2 cut(s)	1617, 1650
MvnI	CGCG	1 cut(s)	1163
MwoI	GCNNNNNNNGC	8 cut(s)	113, 335, 495, 884, 1067, 1377, 1486, 1685
NciI	CCSGG	2 cut(s)	1382, 1755
NcoI	CCATGG	1 cut(s)	1481
NdeII	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
NlaIII	CATG	7 cut(s)	70, 664, 923, 944, 1485, 1513, 1661
NlaIV	GGNNCC	2 cut(s)	1640, 1718
NmuCI	GTSAC	6 cut(s)	651, 675, 797, 1097, 1357, 1551
NsiI	ATGCAT	1 cut(s)	1663
PaeR7I	CTCGAG	2 cut(s)	422, 431
PagI	TCATGA	1 cut(s)	940
PceI	AGGCCT	1 cut(s)	291
PcsI	WCGNNNNNNNCGW	3 cut(s)	420, 429, 450
PdmI	GAANNNNTTC	1 cut(s)	632
PfeI	GAWTC	2 cut(s)	221, 1405
PfoI	TCCNGGA	1 cut(s)	1753
PinAI	ACCGGT	2 cut(s)	1340, 1672
PkrI	GCNGC	7 cut(s)	214, 429, 535, 1072, 1294, 1429, 1681
PleI	GAGTC	2 cut(s)	1523, 1752
PpsI	GAGTC	2 cut(s)	1523, 1752
PsiI	TTATAA	1 cut(s)	323
Psp1406I	AACGTT	1 cut(s)	975
Psp6I	CCWGG	2 cut(s)	1615, 1648
PspEI	GGTNACC	2 cut(s)	675, 1551
PspFI	CCCAGC	1 cut(s)	929
PspGI	CCWGG	2 cut(s)	1615, 1648
PspN4I	GGNNCC	2 cut(s)	1640, 1718
PspPI	GGNCC	6 cut(s)	155, 688, 1044, 1435, 1613, 1639
PspXI	VCTCGAGB	2 cut(s)	422, 431
PstNI	CAGNNNCTG	1 cut(s)	1070
PsuI	RGATCY	1 cut(s)	908
RsaI	GTAC	4 cut(s)	253, 481, 841, 1460
RsaNI	GTAC	4 cut(s)	252, 480, 840, 1459
SalI	GTCGAC	1 cut(s)	308
SaqAI	TTAA	4 cut(s)	951, 983, 1577, 1764
SatI	GCNGC	7 cut(s)	213, 428, 534, 1071, 1293, 1428, 1680
Sau3AI	GATC	5 cut(s)	75, 908, 1123, 1384, 1750
Sau96I	GGNCC	6 cut(s)	155, 688, 1044, 1435, 1613, 1639
ScaI	AGTACT	1 cut(s)	253
SchI	GAGTC	2 cut(s)	1523, 1752
ScrFI	CCNGG	4 cut(s)	1382, 1617, 1650, 1755
SfaNI	GCATC	4 cut(s)	417, 1175, 1476, 1670
SfcI	CTRYAG	1 cut(s)	1240
Sfr274I	CTCGAG	2 cut(s)	422, 431
SinI	GGWCC	4 cut(s)	155, 1044, 1435, 1639
SlaI	CTCGAG	2 cut(s)	422, 431
SmlI	CTYRAG	3 cut(s)	422, 431, 569
SmoI	CTYRAG	3 cut(s)	422, 431, 569
SpeI	ACTAGT	1 cut(s)	668
Sse9I	AATT	9 cut(s)	140, 258, 632, 915, 984, 1306, 1398, 1419, 1606
SseBI	AGGCCT	1 cut(s)	291
SsiI	CCGC	2 cut(s)	342, 1163
SspI	AATATT	1 cut(s)	178
SspMI	CTAG	5 cut(s)	560, 669, 705, 782, 1377
StuI	AGGCCT	1 cut(s)	291
StyD4I	CCNGG	4 cut(s)	1380, 1615, 1648, 1753
StyI	CCWWGG	2 cut(s)	1481, 1556
TaaI	ACNGT	7 cut(s)	440, 479, 817, 1066, 1267, 1504, 1696
TaiI	ACGT	2 cut(s)	978, 1469
TaqI	TCGA	7 cut(s)	309, 423, 432, 444, 453, 1337, 1403
TasI	AATT	9 cut(s)	140, 258, 632, 915, 984, 1306, 1398, 1419, 1606
TatI	WGTACW	1 cut(s)	251
TfiI	GAWTC	2 cut(s)	221, 1405
Tru1I	TTAA	4 cut(s)	951, 983, 1577, 1764
Tru9I	TTAA	4 cut(s)	951, 983, 1577, 1764
TscAI	CASTG	7 cut(s)	508, 535, 1179, 1399, 1649, 1699, 1741
TseFI	GTSAC	6 cut(s)	651, 675, 797, 1097, 1357, 1551
TseI	GCWGC	7 cut(s)	212, 427, 533, 1070, 1292, 1427, 1679
Tsp45I	GTSAC	6 cut(s)	651, 675, 797, 1097, 1357, 1551
TspDTI	ATGAA	9 cut(s)	220, 625, 641, 649, 908, 957, 1127, 1264, 1696
TspGWI	ACGGA	3 cut(s)	1098, 1180, 1275
TspRI	CASTG	7 cut(s)	508, 535, 1179, 1399, 1649, 1699, 1741
VpaK11BI	GGWCC	4 cut(s)	155, 1044, 1435, 1639
XagI	CCTNNNNNAGG	1 cut(s)	1560
XapI	RAATTY	3 cut(s)	140, 1398, 1419
XbaI	TCTAGA	2 cut(s)	559, 781
XhoI	CTCGAG	2 cut(s)	422, 431
XmiI	GTMKAC	2 cut(s)	309, 591
XmnI	GAANNNNTTC	1 cut(s)	632
XspI	CTAG	5 cut(s)	560, 669, 705, 782, 1377
ZrmI	AGTACT	1 cut(s)	253
Zsp2I	ATGCAT	1 cut(s)	1663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.