Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0011630

Physical Location & Seq

Genomic Coordinates Forward (+)

3359226 .. 3362699

Gene Structure 3474 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1029600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1773 bp

ATGTTCACAATACTGAGTTCCTCCAATAATATTGGAGAAGACAGAAGCAAAACACACACTTTCTTTCTAACCATGTTCTTGATCATACAAATCTTGCTGCCATCAGCAGAAGCTAATCAGGCTTCTTCACCATCCCTTTTCTCCAAATTCTCCAGAGCAGGACCATCCAAAGAAGCCAACAATATTGTATCTTTTTGCAAACATACCCCTCACCAAGCAGCTTGTGAATCCATTTTCATCTCCAAAACAAGTAATATTACTACAATTACTAAACCACCCCCTCAAACCCTTGAAGGCCTCCTTGTCCACTCAGTCGACTTCAGCATTATAAAGGCTCGTTTGGCTCGCGCTCTTGCCTATAATCTTACCATTTCCCATTTTCAAACCTCTCATCAAACACACTCGCTTGGTGGCATCAATGACTGCCTCGAGCTGCTCGAGGACAGTATCGATTTACTTGATAATGTCATACTCAAATACGACAGTACCAAAAGCGCCTATGGCCATACTGACGACGATATCCAAAGCTGGCTCAGTGCTGCTCTTACCAATCAAGAAACTTGTCTAGACAGCCTTAAGAGTGATAAGTCAATGGTAGACAAGGGCGCTATGGAGACTTCAGCTAAAAATATGAACCAATTCATAAGTAACTCTCTGTCACTTTTCATGCTCACTAGTAGGTCACCAAAGCAGGCCCAAAACAAAAACCCTAGTAACATTGTCGGTGGCCGGAGGCTGTTGTCGGATGATTTTCCGAGTTGGGTGTCCGGCGCTGAAAGAAAGCTTCTAGAAGCTTCTGTGGGTGACTTAGAAGCACATGCTGTGGTGGCTTTGGATGGGAGTGGTACGCACAAGAGTATCGGTGAAGCACTTGGGTTGGTGGCCTCATTGGCTGAGAGTGGAGGAGGTGGCAGGTCTGTAATTCATGTAAAAGCTGGAACTTATCATGAATATATTAAAATACCAACGAAGCAGAAGAACGTTATGTTAATTGGTGATGGTTTGGGGACGACGATTATCGTGGGCGATAGGAACTCTGATGATGGTTGGACCACTTTCGAGTCTGCTACAGTTGCTGCTATGGGAGACGGCTTTATAGCTCGTGACATAACGTTTGAGAATAATGCCGGTCCATCAAAGCACCAAGCGGTTGCCCTCCGAGTCGGCGCTGACAAGTCAGTGATTTTCCGATGCTCCATTGTCGGCTACCAAGACACCCTCTACACTCACTCCAAGCGCCAGTTCTACAGAGACACCCAAATCTACGGAACAGTTGATTTCATATTTGGAAACTCAGCAGCAGTTTTCCAGAACTGCAACATTTACGCAAGAAAACCCGCTTCAACCGGTCTCAAAAATTTTGTCACAGCACAAGGTAGAACCAGCCCGGATCAGAACACTGGAATTTCGATTCATAACTGCAAAATTTCAGCTGCTTCGGATCTTGCTCCGGTGAAATCCAAGTACGAGACGTATCTTGGGAGGCCATGGAAGCAGTATTCAAGAACAGTTATCATGCAGTCTTACTTGGATGACTCAATCAGTAGGGCTGGTTGGTCACCTTGGTCAGGTGGGTTTGCTTTAAACACATTGTATTATGGTGAGTATTCAAATTATGGGCCTGGGGCTTCAACTTCGGGTCGGGTCCGGTGGCCGGGATATCATGCATCACTTACACCGGTTGCTGCACAAGGCTTCACTGTTAGCGGTTTCATCTCTGGGAACCTTTGGTTGCCTTCCACTGGGGTTTCTTTTGATTCGGGACTTCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

591

Amino Acids

63.91

Weight (kDa)

7.67

Isoelectric Point (pI)

33.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	61 - 221	3.3e-25	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	274 - 575	1.5e-137	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011630)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43050 AT3G59010
fragaria_vesca	FvH4_4g12140
malus_domestica	MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica	Prupe.1G178400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0409791
rosa_laevigata	RLG00000008522
rosa_multiflora	Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354340
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana	Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	329
Acc36I	ACCTGC	1 cut(s)	903
AccI	GTMKAC	2 cut(s)	315, 597
AccII	CGCG	1 cut(s)	348
AciI	CCGC	3 cut(s)	1148, 1338, 1707
AclI	AACGTT	2 cut(s)	981, 1112
AclWI	GGATC	2 cut(s)	1398, 1449
AcoI	YGGCCR	3 cut(s)	502, 727, 1652
AcsI	RAATTY	4 cut(s)	146, 1357, 1404, 1425
AcuI	CTGAAG	2 cut(s)	304, 603
AfaI	GTAC	3 cut(s)	487, 847, 1466
AfiI	CCNNNNNNNGG	3 cut(s)	1568, 1654, 1742
AflII	CTTAAG	1 cut(s)	575
AgeI	ACCGGT	2 cut(s)	1346, 1678
AgsI	TTSAA	6 cut(s)	293, 383, 1344, 1503, 1611, 1632
AhlI	ACTAGT	1 cut(s)	674
AjnI	CCWGG	1 cut(s)	1621
AjuI	GAANNNNNNNTTGG	2 cut(s)	1226, 1258
Alw26I	GTCTC	5 cut(s)	608, 1080, 1245, 1355, 1463
AlwI	GGATC	2 cut(s)	1398, 1449
AlwNI	CAGNNNCTG	1 cut(s)	1076
Ama87I	CYCGRG	2 cut(s)	428, 437
AoxI	GGCC	8 cut(s)	295, 502, 693, 727, 882, 1484, 1619, 1652
ApeKI	GCWGC	8 cut(s)	97, 218, 433, 539, 1076, 1298, 1433, 1685
ApoI	RAATTY	4 cut(s)	146, 1357, 1404, 1425
ArsI	GACNNNNNNTTYG	4 cut(s)	1097, 1129, 1347, 1379
AsiGI	ACCGGT	2 cut(s)	1346, 1678
Asp700I	GAANNNNTTC	1 cut(s)	638
AspLEI	GCGC	6 cut(s)	350, 497, 608, 773, 1169, 1239
AspS9I	GGNCC	6 cut(s)	161, 694, 1050, 1130, 1619, 1645
AsuC2I	CCSGG	2 cut(s)	1388, 1656
AsuHPI	GGTGA	9 cut(s)	120, 203, 675, 815, 875, 1007, 1465, 1551, 1613
AvaI	CYCGRG	2 cut(s)	428, 437
AvaII	GGWCC	4 cut(s)	161, 1050, 1130, 1645
BaeI	ACNNNNGTAYC	4 cut(s)	469, 502, 841, 874
BalI	TGGCCA	1 cut(s)	504
BauI	CACGAG	1 cut(s)	1101
BbsI	GAAGAC	1 cut(s)	45
BbvI	GCAGC	8 cut(s)	84, 230, 420, 526, 1063, 1310, 1420, 1672
BccI	CCATC	7 cut(s)	109, 139, 172, 830, 992, 1037, 1141
BceAI	ACGGC	1 cut(s)	1105
BciT130I	CCWGG	1 cut(s)	1623
BclI	TGATCA	1 cut(s)	81
BcnI	CCSGG	2 cut(s)	1388, 1656
BcoDI	GTCTC	5 cut(s)	608, 1080, 1245, 1355, 1463
BcuI	ACTAGT	1 cut(s)	674
BfaI	CTAG	4 cut(s)	566, 675, 711, 788
BfmI	CTRYAG	2 cut(s)	1068, 1246
BfoI	RGCGCY	5 cut(s)	498, 609, 774, 1170, 1240
BfrI	CTTAAG	1 cut(s)	575
BfuAI	ACCTGC	1 cut(s)	903
BglI	GCCNNNNNGGC	1 cut(s)	890
BisI	GCNGC	8 cut(s)	98, 219, 434, 540, 1077, 1299, 1434, 1686
BlsI	GCNGC	8 cut(s)	99, 220, 435, 541, 1078, 1300, 1435, 1687
Bme1390I	CCNGG	3 cut(s)	1388, 1623, 1656
Bme18I	GGWCC	4 cut(s)	161, 1050, 1130, 1645
BmeT110I	CYCGRG	2 cut(s)	428, 437
BmgT120I	GGNCC	6 cut(s)	161, 694, 1050, 1130, 1619, 1645
BmiI	GGNNCC	2 cut(s)	1646, 1724
BmrFI	CCNGG	3 cut(s)	1388, 1623, 1656
BmrI	ACTGGG	1 cut(s)	1752
BmsI	GCATC	3 cut(s)	423, 1181, 1676
BmuI	ACTGGG	1 cut(s)	1752
BpiI	GAAGAC	1 cut(s)	45
BpmI	CTGGAG	1 cut(s)	136
BpuMI	CCSGG	2 cut(s)	1388, 1656
Bsa29I	ATCGAT	1 cut(s)	450
BsaI	GGTCTC	1 cut(s)	1355
BsaJI	CCNNGG	3 cut(s)	1487, 1562, 1622
BsaWI	WCCGGW	4 cut(s)	1346, 1450, 1647, 1678
BsaXI	ACNNNNNCTCC	2 cut(s)	1214, 1244
Bsc4I	CCNNNNNNNGG	3 cut(s)	1568, 1654, 1742
Bse118I	RCCGGY	3 cut(s)	1127, 1346, 1678
Bse1I	ACTGG	3 cut(s)	1240, 1405, 1747
BseBI	CCWGG	1 cut(s)	1623
BseCI	ATCGAT	1 cut(s)	450
BseDI	CCNNGG	3 cut(s)	1487, 1562, 1622
BseGI	GGATG	5 cut(s)	131, 164, 751, 841, 1537
BseLI	CCNNNNNNNGG	3 cut(s)	1568, 1654, 1742
BseMII	CTCAG	5 cut(s)	5, 324, 547, 885, 1308
BseNI	ACTGG	3 cut(s)	1240, 1405, 1747
BseRI	GAGGAG	1 cut(s)	918
BseXI	GCAGC	8 cut(s)	84, 230, 420, 526, 1063, 1310, 1420, 1672
BsgI	GTGCAG	1 cut(s)	1671
Bsh1236I	CGCG	1 cut(s)	348
BshFI	GGCC	8 cut(s)	297, 504, 695, 729, 884, 1486, 1621, 1654
BshTI	ACCGGT	2 cut(s)	1346, 1678
BshVI	ATCGAT	1 cut(s)	450
BsiHKCI	CYCGRG	2 cut(s)	428, 437
BsiSI	CCGG	9 cut(s)	730, 768, 1128, 1347, 1388, 1451, 1648, 1655, 1679
BslFI	GGGAC	1 cut(s)	1021
BslI	CCNNNNNNNGG	3 cut(s)	1568, 1654, 1742
BsmAI	GTCTC	5 cut(s)	608, 1080, 1245, 1355, 1463
BsmBI	CGTCTC	2 cut(s)	1080, 1463
BsmFI	GGGAC	1 cut(s)	1021
BsnI	GGCC	8 cut(s)	297, 504, 695, 729, 884, 1486, 1621, 1654
Bso31I	GGTCTC	1 cut(s)	1355
BsoBI	CYCGRG	2 cut(s)	428, 437
Bsp143I	GATC	3 cut(s)	81, 1390, 1441
Bsp19I	CCATGG	1 cut(s)	1487
BspACI	CCGC	3 cut(s)	1148, 1338, 1707
BspANI	GGCC	8 cut(s)	297, 504, 695, 729, 884, 1486, 1621, 1654
BspCNI	CTCAG	5 cut(s)	6, 323, 546, 886, 1307
BspDI	ATCGAT	1 cut(s)	450
BspFNI	CGCG	1 cut(s)	348
BspHI	TCATGA	1 cut(s)	946
BspLI	GGNNCC	2 cut(s)	1646, 1724
BspMI	ACCTGC	1 cut(s)	903
BspPI	GGATC	2 cut(s)	1398, 1449
BspTI	CTTAAG	1 cut(s)	575
BspTNI	GGTCTC	1 cut(s)	1355
BsrFI	RCCGGY	3 cut(s)	1127, 1346, 1678
BsrI	ACTGG	3 cut(s)	1240, 1405, 1747
BssAI	RCCGGY	3 cut(s)	1127, 1346, 1678
BssECI	CCNNGG	3 cut(s)	1487, 1562, 1622
BssMI	GATC	3 cut(s)	81, 1390, 1441
BssSI	CACGAG	1 cut(s)	1101
BssT1I	CCWWGG	2 cut(s)	1487, 1562
Bst2BI	CACGAG	1 cut(s)	1101
Bst2UI	CCWGG	1 cut(s)	1623
Bst4CI	ACNGT	6 cut(s)	446, 485, 1072, 1273, 1510, 1702
BstAFI	CTTAAG	1 cut(s)	575
BstC8I	GCNNGC	3 cut(s)	346, 530, 693
BstDEI	CTNAG	6 cut(s)	14, 310, 533, 808, 894, 1294
BstDSI	CCRYGG	1 cut(s)	1487
BstEII	GGTNACC	2 cut(s)	681, 1557
BstENI	CCTNNNNNAGG	1 cut(s)	1566
BstF5I	GGATG	5 cut(s)	131, 164, 751, 841, 1537
BstFNI	CGCG	1 cut(s)	348
BstH2I	RGCGCY	5 cut(s)	498, 609, 774, 1170, 1240
BstHHI	GCGC	6 cut(s)	350, 497, 608, 773, 1169, 1239
BstKTI	GATC	3 cut(s)	84, 1393, 1444
BstMAI	GTCTC	5 cut(s)	608, 1080, 1245, 1355, 1463
BstMBI	GATC	3 cut(s)	81, 1390, 1441
BstMWI	GCNNNNNNNGC	7 cut(s)	119, 341, 501, 827, 890, 1073, 1492
BstNI	CCWGG	1 cut(s)	1623
BstNSI	RCATGY	1 cut(s)	821
BstPI	GGTNACC	2 cut(s)	681, 1557
BstSCI	CCNGG	3 cut(s)	1386, 1621, 1654
BstSFI	CTRYAG	2 cut(s)	1068, 1246
BstUI	CGCG	1 cut(s)	348
BstV1I	GCAGC	8 cut(s)	84, 230, 420, 526, 1063, 1310, 1420, 1672
BstV2I	GAAGAC	1 cut(s)	45
BstX2I	RGATCY	1 cut(s)	1441
BstYI	RGATCY	1 cut(s)	1441
Bsu15I	ATCGAT	1 cut(s)	450
BsuRI	GGCC	8 cut(s)	297, 504, 695, 729, 884, 1486, 1621, 1654
BsuTUI	ATCGAT	1 cut(s)	450
BtgI	CCRYGG	1 cut(s)	1487
BtsCI	GGATG	5 cut(s)	131, 164, 751, 841, 1537
BtsIMutI	CAGTG	5 cut(s)	541, 1185, 1398, 1698, 1740
BveI	ACCTGC	1 cut(s)	903
Cac8I	GCNNGC	3 cut(s)	346, 530, 693
CaiI	CAGNNNCTG	1 cut(s)	1076
CciI	TCATGA	1 cut(s)	946
CfoI	GCGC	6 cut(s)	350, 497, 608, 773, 1169, 1239
Cfr10I	RCCGGY	3 cut(s)	1127, 1346, 1678
Cfr13I	GGNCC	6 cut(s)	161, 694, 1050, 1130, 1619, 1645
ClaI	ATCGAT	1 cut(s)	450
Csp6I	GTAC	3 cut(s)	486, 846, 1465
CspAI	ACCGGT	2 cut(s)	1346, 1678
CviAII	CATG	8 cut(s)	73, 667, 818, 926, 947, 1488, 1516, 1664
CviQI	GTAC	3 cut(s)	486, 846, 1465
DdeI	CTNAG	6 cut(s)	14, 310, 533, 808, 894, 1294
DpnI	GATC	3 cut(s)	83, 1392, 1443
DpnII	GATC	3 cut(s)	81, 1390, 1441
DraI	TTTAAA	1 cut(s)	1584
EaeI	YGGCCR	3 cut(s)	502, 727, 1652
Eco130I	CCWWGG	2 cut(s)	1487, 1562
Eco147I	AGGCCT	1 cut(s)	297
Eco31I	GGTCTC	1 cut(s)	1355
Eco32I	GATATC	2 cut(s)	520, 1661
Eco47I	GGWCC	4 cut(s)	161, 1050, 1130, 1645
Eco57I	CTGAAG	2 cut(s)	304, 603
Eco88I	CYCGRG	2 cut(s)	428, 437
Eco91I	GGTNACC	2 cut(s)	681, 1557
EcoNI	CCTNNNNNAGG	1 cut(s)	1566
EcoO65I	GGTNACC	2 cut(s)	681, 1557
EcoRII	CCWGG	1 cut(s)	1621
EcoRV	GATATC	2 cut(s)	520, 1661
EcoT14I	CCWWGG	2 cut(s)	1487, 1562
EcoT22I	ATGCAT	1 cut(s)	1669
ErhI	CCWWGG	2 cut(s)	1487, 1562
Esp3I	CGTCTC	2 cut(s)	1080, 1463
FaeI	CATG	8 cut(s)	76, 670, 821, 929, 950, 1491, 1519, 1667
FalI	AAGNNNNNCTT	2 cut(s)	285, 317
FaqI	GGGAC	1 cut(s)	1021
FatI	CATG	8 cut(s)	72, 666, 817, 925, 946, 1487, 1515, 1663
FauI	CCCGC	1 cut(s)	1345
FbaI	TGATCA	1 cut(s)	81
FblI	GTMKAC	2 cut(s)	315, 597
Fnu4HI	GCNGC	8 cut(s)	98, 219, 434, 540, 1077, 1299, 1434, 1686
FokI	GGATG	5 cut(s)	118, 151, 758, 848, 1544
Fsp4HI	GCNGC	8 cut(s)	98, 219, 434, 540, 1077, 1299, 1434, 1686
FspBI	CTAG	4 cut(s)	566, 675, 711, 788
GlaI	GCGC	6 cut(s)	349, 496, 607, 772, 1168, 1238
GluI	GCNGC	8 cut(s)	98, 219, 434, 540, 1077, 1299, 1434, 1686
GsuI	CTGGAG	1 cut(s)	136
HaeII	RGCGCY	5 cut(s)	498, 609, 774, 1170, 1240
HaeIII	GGCC	8 cut(s)	297, 504, 695, 729, 884, 1486, 1621, 1654
HapII	CCGG	9 cut(s)	730, 768, 1128, 1347, 1388, 1451, 1648, 1655, 1679
HhaI	GCGC	6 cut(s)	350, 497, 608, 773, 1169, 1239
Hin1II	CATG	8 cut(s)	76, 670, 821, 929, 950, 1491, 1519, 1667
Hin6I	GCGC	6 cut(s)	348, 495, 606, 771, 1167, 1237
HinP1I	GCGC	6 cut(s)	348, 495, 606, 771, 1167, 1237
HincII	GTYRAC	1 cut(s)	316
HindII	GTYRAC	1 cut(s)	316
HindIII	AAGCTT	2 cut(s)	782, 792
HinfI	GANTC	6 cut(s)	227, 1061, 1161, 1411, 1535, 1757
HpaII	CCGG	9 cut(s)	730, 768, 1128, 1347, 1388, 1451, 1648, 1655, 1679
HphI	GGTGA	9 cut(s)	120, 203, 675, 815, 875, 1007, 1465, 1551, 1613
Hpy166II	GTNNAC	4 cut(s)	6, 307, 316, 598
Hpy188I	TCNGA	7 cut(s)	745, 756, 1039, 1160, 1190, 1395, 1441
Hpy8I	GTNNAC	4 cut(s)	6, 307, 316, 598
Hpy99I	CGWCG	2 cut(s)	518, 1015
HpyAV	CCTTC	2 cut(s)	287, 1746
HpyCH4III	ACNGT	6 cut(s)	446, 485, 1072, 1273, 1510, 1702
HpyCH4IV	ACGT	3 cut(s)	981, 1112, 1472
HpyCH4V	TGCA	6 cut(s)	198, 1317, 1422, 1519, 1667, 1688
HpyF10VI	GCNNNNNNNGC	7 cut(s)	119, 341, 501, 827, 890, 1073, 1492
HpyF3I	CTNAG	6 cut(s)	14, 310, 533, 808, 894, 1294
HpySE526I	ACGT	3 cut(s)	981, 1112, 1472
Hsp92II	CATG	8 cut(s)	76, 670, 821, 929, 950, 1491, 1519, 1667
HspAI	GCGC	6 cut(s)	348, 495, 606, 771, 1167, 1237
Ksp22I	TGATCA	1 cut(s)	81
Kzo9I	GATC	3 cut(s)	81, 1390, 1441
LmnI	GCTCC	2 cut(s)	1199, 1453
Lsp1109I	GCAGC	8 cut(s)	84, 230, 420, 526, 1063, 1310, 1420, 1672
LweI	GCATC	3 cut(s)	423, 1181, 1676
MaeI	CTAG	4 cut(s)	566, 675, 711, 788
MaeII	ACGT	3 cut(s)	981, 1112, 1472
MaeIII	GTNAC	8 cut(s)	647, 657, 681, 713, 803, 1103, 1363, 1557
MalI	GATC	3 cut(s)	83, 1392, 1443
MboI	GATC	3 cut(s)	81, 1390, 1441
MboII	GAAGA	3 cut(s)	50, 117, 988
MflI	RGATCY	1 cut(s)	1441
MlsI	TGGCCA	1 cut(s)	504
MluCI	AATT	9 cut(s)	146, 264, 638, 921, 990, 1357, 1404, 1425, 1612
MluNI	TGGCCA	1 cut(s)	504
MlyI	GAGTC	3 cut(s)	1070, 1170, 1529
MmeI	TCCRAC	2 cut(s)	723, 1028
Mox20I	TGGCCA	1 cut(s)	504
Mph1103I	ATGCAT	1 cut(s)	1669
MroXI	GAANNNNTTC	1 cut(s)	638
MscI	TGGCCA	1 cut(s)	504
MseI	TTAA	5 cut(s)	576, 957, 989, 1583, 1771
Msp20I	TGGCCA	1 cut(s)	504
MspA1I	CMGCKG	1 cut(s)	1433
MspCI	CTTAAG	1 cut(s)	575
MspI	CCGG	9 cut(s)	730, 768, 1128, 1347, 1388, 1451, 1648, 1655, 1679
MspR9I	CCNGG	3 cut(s)	1388, 1623, 1656
MvaI	CCWGG	1 cut(s)	1623
MvnI	CGCG	1 cut(s)	348
MwoI	GCNNNNNNNGC	7 cut(s)	119, 341, 501, 827, 890, 1073, 1492
NciI	CCSGG	2 cut(s)	1388, 1656
NcoI	CCATGG	1 cut(s)	1487
NdeII	GATC	3 cut(s)	81, 1390, 1441
NlaIII	CATG	8 cut(s)	76, 670, 821, 929, 950, 1491, 1519, 1667
NlaIV	GGNNCC	2 cut(s)	1646, 1724
NmuCI	GTSAC	6 cut(s)	657, 681, 803, 1103, 1363, 1557
NsiI	ATGCAT	1 cut(s)	1669
NspI	RCATGY	1 cut(s)	821
PaeR7I	CTCGAG	2 cut(s)	428, 437
PagI	TCATGA	1 cut(s)	946
PceI	AGGCCT	1 cut(s)	297
PcsI	WCGNNNNNNNCGW	1 cut(s)	435
PdmI	GAANNNNTTC	1 cut(s)	638
PfeI	GAWTC	3 cut(s)	227, 1411, 1757
PinAI	ACCGGT	2 cut(s)	1346, 1678
PkrI	GCNGC	8 cut(s)	99, 220, 435, 541, 1078, 1300, 1435, 1687
PleI	GAGTC	3 cut(s)	1069, 1169, 1529
PpsI	GAGTC	3 cut(s)	1069, 1169, 1529
PsiI	TTATAA	1 cut(s)	329
Psp1406I	AACGTT	2 cut(s)	981, 1112
Psp6I	CCWGG	1 cut(s)	1621
PspEI	GGTNACC	2 cut(s)	681, 1557
PspGI	CCWGG	1 cut(s)	1621
PspN4I	GGNNCC	2 cut(s)	1646, 1724
PspPI	GGNCC	6 cut(s)	161, 694, 1050, 1130, 1619, 1645
PspXI	VCTCGAGB	2 cut(s)	428, 437
PstNI	CAGNNNCTG	1 cut(s)	1076
PsuI	RGATCY	1 cut(s)	1441
PvuII	CAGCTG	1 cut(s)	1433
RsaI	GTAC	3 cut(s)	487, 847, 1466
RsaNI	GTAC	3 cut(s)	486, 846, 1465
SalI	GTCGAC	1 cut(s)	314
SaqAI	TTAA	5 cut(s)	576, 957, 989, 1583, 1771
SatI	GCNGC	8 cut(s)	98, 219, 434, 540, 1077, 1299, 1434, 1686
Sau3AI	GATC	3 cut(s)	81, 1390, 1441
Sau96I	GGNCC	6 cut(s)	161, 694, 1050, 1130, 1619, 1645
SchI	GAGTC	3 cut(s)	1070, 1170, 1529
ScrFI	CCNGG	3 cut(s)	1388, 1623, 1656
SfaNI	GCATC	3 cut(s)	423, 1181, 1676
SfcI	CTRYAG	2 cut(s)	1068, 1246
Sfr274I	CTCGAG	2 cut(s)	428, 437
SinI	GGWCC	4 cut(s)	161, 1050, 1130, 1645
SlaI	CTCGAG	2 cut(s)	428, 437
SmlI	CTYRAG	3 cut(s)	428, 437, 575
SmoI	CTYRAG	3 cut(s)	428, 437, 575
SpeI	ACTAGT	1 cut(s)	674
Sse9I	AATT	9 cut(s)	146, 264, 638, 921, 990, 1357, 1404, 1425, 1612
SseBI	AGGCCT	1 cut(s)	297
SsiI	CCGC	3 cut(s)	1148, 1338, 1707
SspI	AATATT	3 cut(s)	31, 184, 256
SspMI	CTAG	4 cut(s)	566, 675, 711, 788
StuI	AGGCCT	1 cut(s)	297
StyD4I	CCNGG	3 cut(s)	1386, 1621, 1654
StyI	CCWWGG	2 cut(s)	1487, 1562
TaaI	ACNGT	6 cut(s)	446, 485, 1072, 1273, 1510, 1702
TaiI	ACGT	3 cut(s)	984, 1115, 1475
TaqI	TCGA	6 cut(s)	315, 429, 438, 450, 1059, 1409
TasI	AATT	9 cut(s)	146, 264, 638, 921, 990, 1357, 1404, 1425, 1612
TfiI	GAWTC	3 cut(s)	227, 1411, 1757
Tru1I	TTAA	5 cut(s)	576, 957, 989, 1583, 1771
Tru9I	TTAA	5 cut(s)	576, 957, 989, 1583, 1771
TscAI	CASTG	5 cut(s)	541, 1185, 1405, 1705, 1747
TseFI	GTSAC	6 cut(s)	657, 681, 803, 1103, 1363, 1557
TseI	GCWGC	8 cut(s)	97, 218, 433, 539, 1076, 1298, 1433, 1685
Tsp45I	GTSAC	6 cut(s)	657, 681, 803, 1103, 1363, 1557
TspGWI	ACGGA	1 cut(s)	1281
TspRI	CASTG	5 cut(s)	541, 1185, 1405, 1705, 1747
Vha464I	CTTAAG	1 cut(s)	575
VpaK11BI	GGWCC	4 cut(s)	161, 1050, 1130, 1645
XagI	CCTNNNNNAGG	1 cut(s)	1566
XapI	RAATTY	4 cut(s)	146, 1357, 1404, 1425
XbaI	TCTAGA	2 cut(s)	565, 787
XceI	RCATGY	1 cut(s)	821
XhoI	CTCGAG	2 cut(s)	428, 437
XmiI	GTMKAC	2 cut(s)	315, 597
XmnI	GAANNNNTTC	1 cut(s)	638
XspI	CTAG	4 cut(s)	566, 675, 711, 788
Zsp2I	ATGCAT	1 cut(s)	1669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.