Rh4CG168600

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0011630
Physical Location & Seq
Reverse (-)
36963205 .. 36967374
4170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG168600.1

Sequence Viewer

Length: 1599 bp
ATGTTCTTGGCGGTGGAAATTTTATTCTTCTCAAGCATATTATTAGCAAAAGCTTCTCCTTCACAATCCCATTTCTCCAAATTTTCTAGATCATCCGGAGGACTTTCCCATAAAGAAGCCATTTTATCTCTCTGCGCACATACCCCTCACCGAGCAACTTGTGAATCCACTCTCCTCTCCTCCTCCTCATCATCATCATCTACAAGAGGCTCTGATCATCTCCCTCATACTCTAGAAGGTGCCTTTGTCCAGTCGGTCCATTCCACCGTTGCGAAAGCTCAGCACGCTCGAGCTCTCGCCTATAATCTCACTATTTCCCACCGCCAAAATGCCGATCATGATGTTCACTTCCCTGGTCGCGGTGTGAACGACTGCCTTGAGCTTCTCGATGATGCTATAGATATGCTCGACAATGTCATGATGACATCGTTGTCAAAACCTAACGATGACGACGTTCACACCTGGCTTAGTGCAGCTCTTACTAACCAAGAAACTTGTCTAGAGAGCCTCGAGAAAGACAAGTTCAAGACAGAGAAAAACATTATGGATGTCACGGCTCAAAATCTGAGCCAATACATAAGCAACTCCCTAGCACTTTTTGTGCTTACCATGGGTAGTAGCAGTGTCTCCTTCGCTAGTAGTTCGGAGCATTTACCTCGAGACACTGAGTCAAAGTCAAAGGAAGCTAAAAATGCAAACCCTAATAGAAGACTATTGGCATCGGACAGTAGTTTTCCGACGTGGGTCCCGGCCGCAGAAGGGAAGCTTCTAGAAGCTTCTGTTGGGGAGATAGAAGCAGATGCTGTGGTGGCTCAGGATGGGAGTGGGACGCACAAGAGCATTGGTGAGGCACTGAAGGGGTTGCTGCTAATTGGATCCTTGGAGAGTAGTGGTACTAGTACTGGGGGCAGAACTGTGATTTACGTAAAAGCTGGGACATACCACGAGTACATAAACATACCCACGAAGCAAAAGAACGTGATGTTGATTGGTGATGGAGTGGGGAAAACGATCATTGTGGGCAACAGGAACTCAGACGATGGCTGGACAACTTATAAGTCTGCTACTGTGGCTGCTATGGGTGATGGGTTCATCGCTCGTGATATAACATTTGTGAACGATGCCGGCCCTTCCAAGCACCAAGCAGTCGCACTCCGAGTCGGTGCCGACAAATCAGTCGTCCACCGATGTTCCATCATAGGCTACCAGGACACCCTCTACACTCACTCCAAGCGACAGTTCTACCGAGAAACCGACATCTATGGAACCGTCGACTTCATCTTCGGCAACTCAGCCGTGGTTTTCCAAACTTGCAACATCCACCCCCGAAAACCGCCCTCCAGCGGGCTCAAGAACTTTGTCACAGCTCAAGGCCGAAGCAGCCCTAATCAGAACACAGGTATTTCAATCCACAACTGTAAGATAGCGGCGGCATCAGACCTCTTCCCGGTGAGATCCAGCTACGAGACGTATCTTGGGAGACCGTGGAAGCAGTATTCGAGAACGATCATCATGCAGTCTTTCATGGATGACTCGATAAATCGAGCAGGGTGGTCTCCTTGGTCTGTTAAATTCACTACTTCTCTATGTAAATTTTAG

Protein Analysis

532

Amino Acids

57.82

Weight (kDa)

7.32

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 40 - 199 4.5e-26 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 267 - 526 1.6e-113 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1056
AasI GACNNNNNNGTC 2 cut(s) 430, 1175
Acc16I TGCGCA 1 cut(s) 136
AccB1I GGYRCC 2 cut(s) 239, 1163
AccI GTMKAC 1 cut(s) 1272
AccII CGCG 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 95
AciI CCGC 8 cut(s) 11, 322, 360, 753, 1334, 1344, 1427, 1430
AclWI GGATC 3 cut(s) 870, 883, 1449
AcoI YGGCCR 1 cut(s) 750
AcsI RAATTY 4 cut(s) 18, 80, 1571, 1592
AcuI CTGAAG 1 cut(s) 875
AfaI GTAC 3 cut(s) 895, 901, 950
AfiI CCNNNNNNNGG 5 cut(s) 359, 759, 1343, 1344, 1447
AgsI TTSAA 2 cut(s) 526, 1407
AhdI GACNNNNNGTC 1 cut(s) 667
AhlI ACTAGT 1 cut(s) 896
AjiI CACGTC 1 cut(s) 741
AjnI CCWGG 3 cut(s) 352, 461, 1206
AjuI GAANNNNNNNTTGG 4 cut(s) 765, 797, 1223, 1255
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 5 cut(s) 631, 654, 1460, 1474, 1560
AlwI GGATC 3 cut(s) 870, 883, 1449
AlwNI CAGNNNCTG 1 cut(s) 803
Ama87I CYCGRG 3 cut(s) 288, 509, 657
Aor13HI TCCGGA 1 cut(s) 95
AoxI GGCC 3 cut(s) 750, 1126, 1372
ApeKI GCWGC 4 cut(s) 473, 865, 1073, 1380
ApoI RAATTY 4 cut(s) 18, 80, 1571, 1592
ArsI GACNNNNNNTTYG 4 cut(s) 1164, 1196, 1265, 1297
AspLEI GCGC 1 cut(s) 137
AspS9I GGNCC 3 cut(s) 256, 745, 1127
AsuC2I CCSGG 2 cut(s) 749, 1448
AsuHPI GGTGA 5 cut(s) 140, 857, 1004, 1094, 1462
AvaI CYCGRG 3 cut(s) 288, 509, 657
AvaII GGWCC 2 cut(s) 256, 745
BamHI GGATCC 1 cut(s) 875
BanI GGYRCC 2 cut(s) 239, 1163
BanII GRGCYC 2 cut(s) 295, 1350
BauI CACGAG 2 cut(s) 944, 1098
BbsI GAAGAC 1 cut(s) 715
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 4 cut(s) 485, 852, 1060, 1392
BccI CCATC 5 cut(s) 812, 989, 1034, 1079, 1202
BceAI ACGGC 2 cut(s) 570, 1280
BcgI CGANNNNNNTGC 4 cut(s) 638, 672, 1495, 1529
BciT130I CCWGG 3 cut(s) 354, 463, 1208
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 749, 1448
BcoDI GTCTC 5 cut(s) 631, 654, 1460, 1474, 1560
BcuI ACTAGT 1 cut(s) 896
BfaI CTAG 7 cut(s) 87, 233, 500, 590, 636, 770, 897
BfmI CTRYAG 1 cut(s) 396
BisI GCNGC 7 cut(s) 474, 753, 866, 1074, 1381, 1428, 1431
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 7 cut(s) 475, 754, 867, 1075, 1382, 1429, 1432
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 5 cut(s) 354, 463, 749, 1208, 1448
Bme18I GGWCC 2 cut(s) 256, 745
BmeRI GACNNNNNGTC 1 cut(s) 667
BmeT110I CYCGRG 3 cut(s) 288, 509, 657
BmgBI CACGTC 1 cut(s) 741
BmgT120I GGNCC 3 cut(s) 256, 745, 1127
BmiI GGNNCC 6 cut(s) 241, 746, 747, 877, 1165, 1267
BmrFI CCNGG 5 cut(s) 354, 463, 749, 1208, 1448
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 5 cut(s) 382, 728, 790, 1111, 1442
BmuI ACTGGG 1 cut(s) 912
BoxI GACNNNNGTC 1 cut(s) 743
BpiI GAAGAC 1 cut(s) 715
BpmI CTGGAG 1 cut(s) 1324
Bpu10I CCTNAGC 1 cut(s) 813
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 4 cut(s) 16, 398, 1334, 1353
BpuMI CCSGG 2 cut(s) 749, 1448
BsaAI YACGTR 1 cut(s) 925
BsaI GGTCTC 2 cut(s) 1474, 1560
BsaJI CCNNGG 6 cut(s) 352, 609, 879, 1296, 1484, 1559
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 2 cut(s) 1211, 1241
Bsc4I CCNNNNNNNGG 5 cut(s) 359, 759, 1343, 1344, 1447
Bse118I RCCGGY 1 cut(s) 1124
Bse1I ACTGG 2 cut(s) 250, 907
BseAI TCCGGA 1 cut(s) 95
BseBI CCWGG 3 cut(s) 354, 463, 1208
BseDI CCNNGG 6 cut(s) 352, 609, 879, 1296, 1484, 1559
BseGI GGATG 5 cut(s) 92, 553, 823, 1317, 1534
BseLI CCNNNNNNNGG 5 cut(s) 359, 759, 1343, 1344, 1447
BseMII CTCAG 6 cut(s) 293, 557, 657, 827, 1047, 1305
BseNI ACTGG 2 cut(s) 250, 907
BseRI GAGGAG 4 cut(s) 164, 169, 172, 175
BseX3I CGGCCG 1 cut(s) 750
BseXI GCAGC 4 cut(s) 485, 852, 1060, 1392
BseYI CCCAGC 1 cut(s) 932
BsgI GTGCAG 1 cut(s) 492
Bsh1236I CGCG 1 cut(s) 360
Bsh1285I CGRYCG 1 cut(s) 753
BshFI GGCC 3 cut(s) 752, 1128, 1374
BshNI GGYRCC 2 cut(s) 239, 1163
BsiEI CGRYCG 1 cut(s) 753
BsiHKAI GWGCWC 1 cut(s) 295
BsiHKCI CYCGRG 3 cut(s) 288, 509, 657
BsiSI CCGG 4 cut(s) 96, 749, 1125, 1448
BslFI GGGAC 3 cut(s) 731, 841, 949
BslI CCNNNNNNNGG 5 cut(s) 359, 759, 1343, 1344, 1447
BsmAI GTCTC 5 cut(s) 631, 654, 1460, 1474, 1560
BsmBI CGTCTC 1 cut(s) 1460
BsmFI GGGAC 3 cut(s) 731, 841, 949
BsnI GGCC 3 cut(s) 752, 1128, 1374
Bso31I GGTCTC 2 cut(s) 1474, 1560
BsoBI CYCGRG 3 cut(s) 288, 509, 657
Bsp1286I GDGCHC 2 cut(s) 295, 1350
Bsp13I TCCGGA 1 cut(s) 95
Bsp143I GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
Bsp1720I GCTNAGC 1 cut(s) 279
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 8 cut(s) 11, 322, 360, 753, 1334, 1344, 1427, 1430
BspANI GGCC 3 cut(s) 752, 1128, 1374
BspCNI CTCAG 6 cut(s) 292, 558, 658, 826, 1046, 1304
BspEI TCCGGA 1 cut(s) 95
BspFNI CGCG 1 cut(s) 360
BspHI TCATGA 2 cut(s) 337, 417
BspLI GGNNCC 6 cut(s) 241, 746, 747, 877, 1165, 1267
BspPI GGATC 3 cut(s) 870, 883, 1449
BspT107I GGYRCC 2 cut(s) 239, 1163
BspTNI GGTCTC 2 cut(s) 1474, 1560
BsrFI RCCGGY 1 cut(s) 1124
BsrI ACTGG 2 cut(s) 250, 907
BssAI RCCGGY 1 cut(s) 1124
BssECI CCNNGG 6 cut(s) 352, 609, 879, 1296, 1484, 1559
BssMI GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
BssSI CACGAG 2 cut(s) 944, 1098
BssT1I CCWWGG 3 cut(s) 609, 879, 1559
Bst2BI CACGAG 2 cut(s) 944, 1098
Bst2UI CCWGG 3 cut(s) 354, 463, 1208
Bst4CI ACNGT 8 cut(s) 268, 728, 916, 1069, 1239, 1270, 1418, 1485
Bst6I CTCTTC 1 cut(s) 1448
BstBAI YACGTR 1 cut(s) 925
BstC8I GCNNGC 3 cut(s) 285, 1126, 1346
BstDEI CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1033, 1291
BstDSI CCRYGG 3 cut(s) 609, 1296, 1484
BstF5I GGATG 5 cut(s) 92, 553, 823, 1317, 1534
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 7 cut(s) 92, 217, 337, 878, 1014, 1457, 1509
BstMAI GTCTC 5 cut(s) 631, 654, 1460, 1474, 1560
BstMBI GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
BstMCI CGRYCG 1 cut(s) 753
BstMWI GCNNNNNNNGC 5 cut(s) 284, 692, 809, 1070, 1380
BstNI CCWGG 3 cut(s) 354, 463, 1208
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 5 cut(s) 352, 461, 747, 1206, 1446
BstSFI CTRYAG 1 cut(s) 396
BstSNI TACGTA 1 cut(s) 925
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 4 cut(s) 485, 852, 1060, 1392
BstV2I GAAGAC 1 cut(s) 715
BstX2I RGATCY 2 cut(s) 875, 1454
BstYI RGATCY 2 cut(s) 875, 1454
BstZI CGGCCG 1 cut(s) 750
BsuRI GGCC 3 cut(s) 752, 1128, 1374
BtgI CCRYGG 3 cut(s) 609, 1296, 1484
BtgZI GCGATG 1 cut(s) 1078
BtrI CACGTC 1 cut(s) 741
BtsCI GGATG 5 cut(s) 92, 553, 823, 1317, 1534
BtsI GCAGTG 1 cut(s) 628
BtsIMutI CAGTG 3 cut(s) 628, 663, 851
Cac8I GCNNGC 3 cut(s) 285, 1126, 1346
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 2 cut(s) 337, 417
CfoI GCGC 1 cut(s) 137
Cfr10I RCCGGY 1 cut(s) 1124
Cfr13I GGNCC 3 cut(s) 256, 745, 1127
CseI GACGC 1 cut(s) 838
Csp6I GTAC 3 cut(s) 894, 900, 949
CviAII CATG 5 cut(s) 338, 418, 610, 1513, 1525
CviQI GTAC 3 cut(s) 894, 900, 949
DdeI CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1033, 1291
DpnI GATC 7 cut(s) 91, 216, 336, 877, 1013, 1456, 1508
DpnII GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
DrdI GACNNNNNNGTC 2 cut(s) 430, 1175
DriI GACNNNNNGTC 1 cut(s) 667
DseDI GACNNNNNNGTC 2 cut(s) 430, 1175
EaeI YGGCCR 1 cut(s) 750
EagI CGGCCG 1 cut(s) 750
Eam1104I CTCTTC 1 cut(s) 1448
Eam1105I GACNNNNNGTC 1 cut(s) 667
EarI CTCTTC 1 cut(s) 1448
Ecl136II GAGCTC 1 cut(s) 293
EclXI CGGCCG 1 cut(s) 750
Eco105I TACGTA 1 cut(s) 925
Eco130I CCWWGG 3 cut(s) 609, 879, 1559
Eco24I GRGCYC 2 cut(s) 295, 1350
Eco31I GGTCTC 2 cut(s) 1474, 1560
Eco47I GGWCC 2 cut(s) 256, 745
Eco52I CGGCCG 1 cut(s) 750
Eco53kI GAGCTC 1 cut(s) 293
Eco57I CTGAAG 1 cut(s) 875
Eco88I CYCGRG 3 cut(s) 288, 509, 657
EcoICRI GAGCTC 1 cut(s) 293
EcoO109I RGGNCCY 1 cut(s) 745
EcoRII CCWGG 3 cut(s) 352, 461, 1206
EcoT14I CCWWGG 3 cut(s) 609, 879, 1559
EcoT38I GRGCYC 2 cut(s) 295, 1350
ErhI CCWWGG 3 cut(s) 609, 879, 1559
Esp3I CGTCTC 1 cut(s) 1460
FaeI CATG 5 cut(s) 341, 421, 613, 1516, 1528
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 3 cut(s) 731, 841, 949
FatI CATG 5 cut(s) 337, 417, 609, 1512, 1524
FauI CCCGC 1 cut(s) 1337
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 1272
Fnu4HI GCNGC 7 cut(s) 474, 753, 866, 1074, 1381, 1428, 1431
FokI GGATG 5 cut(s) 79, 560, 830, 1304, 1541
FriOI GRGCYC 2 cut(s) 295, 1350
Fsp4HI GCNGC 7 cut(s) 474, 753, 866, 1074, 1381, 1428, 1431
FspBI CTAG 7 cut(s) 87, 233, 500, 590, 636, 770, 897
FspI TGCGCA 1 cut(s) 136
GlaI GCGC 1 cut(s) 136
GluI GCNGC 7 cut(s) 474, 753, 866, 1074, 1381, 1428, 1431
GsaI CCCAGC 1 cut(s) 936
GsuI CTGGAG 1 cut(s) 1324
HaeIII GGCC 3 cut(s) 752, 1128, 1374
HapII CCGG 4 cut(s) 96, 749, 1125, 1448
HgaI GACGC 1 cut(s) 838
HhaI GCGC 1 cut(s) 137
Hin1II CATG 5 cut(s) 341, 421, 613, 1516, 1528
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HincII GTYRAC 1 cut(s) 1273
HindII GTYRAC 1 cut(s) 1273
HindIII AAGCTT 3 cut(s) 51, 764, 774
HinfI GANTC 4 cut(s) 164, 668, 1158, 1532
HpaII CCGG 4 cut(s) 96, 749, 1125, 1448
HphI GGTGA 5 cut(s) 140, 857, 1004, 1094, 1462
Hpy166II GTNNAC 6 cut(s) 346, 367, 457, 1117, 1183, 1273
Hpy188I TCNGA 9 cut(s) 214, 567, 646, 724, 738, 1036, 1157, 1392, 1438
Hpy8I GTNNAC 6 cut(s) 346, 367, 457, 1117, 1183, 1273
Hpy99I CGWCG 3 cut(s) 455, 742, 1274
HpyAV CCTTC 6 cut(s) 69, 230, 640, 752, 850, 1140
HpyCH4III ACNGT 8 cut(s) 268, 728, 916, 1069, 1239, 1270, 1418, 1485
HpyCH4IV ACGT 5 cut(s) 453, 740, 924, 978, 1469
HpyCH4V TGCA 4 cut(s) 473, 695, 1314, 1516
HpyF10VI GCNNNNNNNGC 5 cut(s) 284, 692, 809, 1070, 1380
HpyF3I CTNAG 7 cut(s) 279, 467, 566, 666, 813, 1033, 1291
HpySE526I ACGT 5 cut(s) 453, 740, 924, 978, 1469
Hsp92II CATG 5 cut(s) 341, 421, 613, 1516, 1528
HspAI GCGC 1 cut(s) 135
KflI GGGWCCC 1 cut(s) 745
Kpn2I TCCGGA 1 cut(s) 95
KroI GCCGGC 1 cut(s) 1124
KroNI GCCGGC 1 cut(s) 1126
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
LmnI GCTCC 1 cut(s) 646
Lsp1109I GCAGC 4 cut(s) 485, 852, 1060, 1392
LweI GCATC 5 cut(s) 382, 728, 790, 1111, 1442
MaeI CTAG 7 cut(s) 87, 233, 500, 590, 636, 770, 897
MaeII ACGT 5 cut(s) 453, 740, 924, 978, 1469
MaeIII GTNAC 2 cut(s) 550, 1360
MalI GATC 7 cut(s) 91, 216, 336, 877, 1013, 1456, 1508
MboI GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
MboII GAAGA 4 cut(s) 19, 720, 1273, 1435
MflI RGATCY 2 cut(s) 875, 1454
MhlI GDGCHC 2 cut(s) 295, 1350
MluCI AATT 5 cut(s) 18, 80, 870, 1571, 1592
MlyI GAGTC 3 cut(s) 677, 1167, 1526
MmeI TCCRAC 1 cut(s) 761
MroI TCCGGA 1 cut(s) 95
MroNI GCCGGC 1 cut(s) 1124
MseI TTAA 1 cut(s) 1569
MspA1I CMGCKG 1 cut(s) 1344
MspI CCGG 4 cut(s) 96, 749, 1125, 1448
MspR9I CCNGG 5 cut(s) 354, 463, 749, 1208, 1448
MvaI CCWGG 3 cut(s) 354, 463, 1208
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 5 cut(s) 284, 692, 809, 1070, 1380
NaeI GCCGGC 1 cut(s) 1126
NciI CCSGG 2 cut(s) 749, 1448
NcoI CCATGG 1 cut(s) 609
NdeII GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
NgoMIV GCCGGC 1 cut(s) 1124
NlaIII CATG 5 cut(s) 341, 421, 613, 1516, 1528
NlaIV GGNNCC 6 cut(s) 241, 746, 747, 877, 1165, 1267
NmuCI GTSAC 2 cut(s) 550, 1360
NsbI TGCGCA 1 cut(s) 136
PaeR7I CTCGAG 3 cut(s) 288, 509, 657
PagI TCATGA 2 cut(s) 337, 417
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PdiI GCCGGC 1 cut(s) 1126
PfeI GAWTC 1 cut(s) 164
PflFI GACNNNGTC 1 cut(s) 413
PkrI GCNGC 7 cut(s) 475, 754, 867, 1075, 1382, 1429, 1432
PleI GAGTC 3 cut(s) 676, 1166, 1526
PpsI GAGTC 3 cut(s) 676, 1166, 1526
Ppu21I YACGTR 1 cut(s) 925
PpuMI RGGWCCY 1 cut(s) 745
PshAI GACNNNNGTC 1 cut(s) 743
PsiI TTATAA 1 cut(s) 1056
Psp124BI GAGCTC 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 745
Psp6I CCWGG 3 cut(s) 352, 461, 1206
PspFI CCCAGC 1 cut(s) 932
PspGI CCWGG 3 cut(s) 352, 461, 1206
PspN4I GGNNCC 6 cut(s) 241, 746, 747, 877, 1165, 1267
PspPI GGNCC 3 cut(s) 256, 745, 1127
PspPPI RGGWCCY 1 cut(s) 745
PspXI VCTCGAGB 1 cut(s) 288
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 875, 1454
PsyI GACNNNGTC 1 cut(s) 413
RsaI GTAC 3 cut(s) 895, 901, 950
RsaNI GTAC 3 cut(s) 894, 900, 949
SacI GAGCTC 1 cut(s) 295
SalI GTCGAC 1 cut(s) 1271
SaqAI TTAA 1 cut(s) 1569
SatI GCNGC 7 cut(s) 474, 753, 866, 1074, 1381, 1428, 1431
Sau3AI GATC 7 cut(s) 89, 214, 334, 875, 1011, 1454, 1506
Sau96I GGNCC 3 cut(s) 256, 745, 1127
ScaI AGTACT 1 cut(s) 901
SchI GAGTC 3 cut(s) 677, 1167, 1526
ScrFI CCNGG 5 cut(s) 354, 463, 749, 1208, 1448
SduI GDGCHC 2 cut(s) 295, 1350
SfaNI GCATC 5 cut(s) 382, 728, 790, 1111, 1442
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 3 cut(s) 288, 509, 657
SinI GGWCC 2 cut(s) 256, 745
SlaI CTCGAG 3 cut(s) 288, 509, 657
SmlI CTYRAG 7 cut(s) 31, 288, 377, 509, 657, 1349, 1368
SmoI CTYRAG 7 cut(s) 31, 288, 377, 509, 657, 1349, 1368
SnaBI TACGTA 1 cut(s) 925
SpeI ACTAGT 1 cut(s) 896
Sse9I AATT 5 cut(s) 18, 80, 870, 1571, 1592
SsiI CCGC 8 cut(s) 11, 322, 360, 753, 1334, 1344, 1427, 1430
SspMI CTAG 7 cut(s) 87, 233, 500, 590, 636, 770, 897
SstI GAGCTC 1 cut(s) 295
StyD4I CCNGG 5 cut(s) 352, 461, 747, 1206, 1446
StyI CCWWGG 3 cut(s) 609, 879, 1559
TaaI ACNGT 8 cut(s) 268, 728, 916, 1069, 1239, 1270, 1418, 1485
TaiI ACGT 5 cut(s) 456, 743, 927, 981, 1472
TaqI TCGA 9 cut(s) 289, 387, 408, 510, 658, 1272, 1499, 1535, 1543
TaqII GACCGA 1 cut(s) 244
TasI AATT 5 cut(s) 18, 80, 870, 1571, 1592
TatI WGTACW 2 cut(s) 899, 948
TauI GCSGC 3 cut(s) 755, 1430, 1433
TfiI GAWTC 1 cut(s) 164
Tru1I TTAA 1 cut(s) 1569
Tru9I TTAA 1 cut(s) 1569
TscAI CASTG 3 cut(s) 628, 670, 858
TseFI GTSAC 2 cut(s) 550, 1360
TseI GCWGC 4 cut(s) 473, 865, 1073, 1380
Tsp45I GTSAC 2 cut(s) 550, 1360
TspDTI ATGAA 3 cut(s) 1081, 1267, 1513
TspRI CASTG 3 cut(s) 628, 670, 858
Tth111I GACNNNGTC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 256, 745
XapI RAATTY 4 cut(s) 18, 80, 1571, 1592
XbaI TCTAGA 4 cut(s) 86, 232, 499, 769
XhoI CTCGAG 3 cut(s) 288, 509, 657
XmiI GTMKAC 1 cut(s) 1272
XspI CTAG 7 cut(s) 87, 233, 500, 590, 636, 770, 897
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.