Prupe.1G178400_v2.0.a1

pectinesterase pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0011630
Physical Location & Seq
Forward (+)
15304141 .. 15307207
3067 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G178400.1

Sequence Viewer

Length: 1779 bp
ATGTTGGCTATACTTTCCTCCCATGGAGGACACAGAAGCAAAGCACACACTCTCTTTCTACCTTTGTTCTTGACCATAGAAATTTTATCCTTGTCAAGCTTATTAGCAGAAGCTAACCAAGCTTCTTCACCATCCCATTTCTCCAAATTCTCTAGAGGAGGACCCTCCAAAGAAGCCAACAATAATATTCTATCGTTTTGCAAAAAAACTCCTCACCAAGCAGCTTGTGAATCTATTTTCATCTCCTCATCATCCAAAACAAGTACTACTTATAAAGAAGCCCCTCAAACCCTTGAAAATCTCTTTGTCCACTCAGTCGAATTCAGCATAAGTAAGGCTCATTTGGCTCGAGCCCTTGCCTATAACTTCGCCATTTCCCATCGTCAAAATGCTCACCAACCCCACTTGCTTGGTGGCATCAACGACTGCCTTGAGCTGCTCGAGGACAGTATCGATTCACTCGATAATGTCATACGCAAGCACGACAAAAGTACTAAGAGCAGCACCTATGGCCATGTTGACGATGATGTCCAAACCTGGCTCAGTGCTGCTCTTACCAATCAAGAAACTTGTCTAGAGAGCCTTGAGAATGACAACTCTAAGGTAGACAAGGGCCTCATGGAGACTTCAGCTAAGAACTTGAGCCAATTCATAAGCAACTCCCTATCACTTTTCATGATGACTAGTAGAACTAAAAACACAAACCCTAGGCCGAATGTTGTCGGCGGCCGGAGGCTGTTGTCGGATGATTTTCCGACATGGGTCTCAGGCACGGAGAGGAAGCTTCTAGAAGGTTCTGTGGATGAATTGGAAGCACATGCCGTGGTGGCTTTGGATGGGAGTGGCACGCACAAAAGCATTGGTGAGGCACTTGGACTGGTGGCCTCATTGGAGGAGAGTGGTACTACTGGAGGCAGATCTGTAATTCACGTAAAAGCTGGGACTTATTATGAATACATAAAAATACCCACCAAACAGAAGAACGTTATGTTAATTGGTGATGGATTGGGGAAGACCATTATTGTGGGCAGTAGGAGTTCGGAAGCTGGTTGGACAACTTACCAGTCTGCCACTGTGGCTGCTATGGGTGACGGATTTATAGCACGCGACATAACAATTGTGAATAGCGCCGGCCCTGCAAAGCAACAAGCTGTTGCTCTTCGAGTTGGTGCAGACAAGTCAGTGATCTTCAGATGCTCCATAGTTGGCTACCAAGACACCCTCTACACTCACTCCAAGCGCCAGTTCTACAGAGAAACCCAAATCTATGGAACTGTCGATTTCATATTCGGAAACTCAGCTGTGGTTTTCCAAAACTGCAACATATTTGCAAGAAAGCCTGCATCATCTGGCCACAAAAACTTCGTCACAGCACAAGGCAGAAGCAGCCCAGATCAAAACACAGGCATTTCAATCCATAACTGCAAAATATCAGCTGCATCAGATCTTGCTCCAGTCAAATCCAATTATGAGACATATCTTGGGAGGCCATGGAAGCAGTATTCTAGAACAGTTGTTATGCAGTCTTATTTGGATGAGTCAATAAATAGAGCTGGGTGGTCTCCTTGGGCAGGTGGGTTTGGCCTAACGACTTTGTATTATGGTGAGTACTTGAATTTTGGGCCTGGGGCTTCAACTTCAGGTAGGGTTCAGTGGCCTGGATACCATGCTTCAATTACAACAACTGTAGCTCAAGGTTTTACTGTTGGTGGTTTCATTTCTGGGAACCTTTGGTTGCCTTCCACCGGCGTGTCTTTCGATTCAGGATTGATTAGCTGA

Protein Analysis

593

Amino Acids

64.12

Weight (kDa)

8.3

Isoelectric Point (pI)

31.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011630)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43050 AT3G59010
fragaria_vesca FvH4_4g12140
malus_domestica MD04G1028900.v1.1 MD04G1029600.v1.1 MD04G1030100.v1.1
prunus_persica Prupe.1G178400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0409791
rosa_laevigata RLG00000008522
rosa_multiflora Rmu_sc0005198.1_g000009 Rmu_sc0040314.1_g000001
rosa_roxburghii Rroxscaffold_5G00354340
rosa_rugosa Rorug04G0096600
rosa_samantha Rh4AG158100 Rh4BG157800 Rh4CG168600 Rh4DG152700
rosa_wichuraiana Rw0G017480 Rw4G013060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 273
AarI CACCTGC 1 cut(s) 1562
AasI GACNNNNNNGTC 1 cut(s) 527
Acc36I ACCTGC 1 cut(s) 1562
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 1107
AciI CCGC 1 cut(s) 726
AclI AACGTT 1 cut(s) 984
AcoI YGGCCR 3 cut(s) 511, 727, 1351
AcsI RAATTY 4 cut(s) 81, 146, 320, 1615
AcuI CTGAAG 3 cut(s) 612, 1174, 1623
AfaI GTAC 4 cut(s) 265, 493, 904, 1610
AfiI CCNNNNNNNGG 2 cut(s) 1571, 1745
AgsI TTSAA 5 cut(s) 296, 1413, 1615, 1635, 1674
AhlI ACTAGT 1 cut(s) 683
AjnI CCWGG 3 cut(s) 536, 1624, 1657
AjuI GAANNNNNNNTTGG 2 cut(s) 1229, 1261
AleI CACNNNNGTG 1 cut(s) 1748
Alw26I GTCTC 4 cut(s) 617, 769, 1466, 1566
Ama87I CYCGRG 2 cut(s) 348, 440
ApeKI GCWGC 7 cut(s) 221, 436, 501, 548, 1079, 1386, 1436
ApoI RAATTY 4 cut(s) 81, 146, 320, 1615
ArsI GACNNNNNNTTYG 2 cut(s) 1350, 1382
AspA2I CCTAGG 1 cut(s) 707
AspLEI GCGC 2 cut(s) 1130, 1242
AspS9I GGNCC 4 cut(s) 161, 613, 1133, 1622
AsuHPI GGTGA 7 cut(s) 120, 206, 386, 875, 1010, 1100, 1616
AvaI CYCGRG 2 cut(s) 348, 440
AvaII GGWCC 1 cut(s) 161
AvrII CCTAGG 1 cut(s) 707
BalI TGGCCA 2 cut(s) 513, 1353
BanII GRGCYC 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 1019
BbvI GCAGC 7 cut(s) 233, 423, 513, 535, 1066, 1398, 1423
BccI CCATC 4 cut(s) 139, 387, 830, 995
BceAI ACGGC 1 cut(s) 806
BciT130I CCWGG 3 cut(s) 538, 1626, 1659
BciVI GTATCC 1 cut(s) 1655
BcoDI GTCTC 4 cut(s) 617, 769, 1466, 1566
BcuI ACTAGT 1 cut(s) 683
BfaI CTAG 6 cut(s) 153, 575, 684, 708, 788, 1506
BfmI CTRYAG 2 cut(s) 1249, 1686
BfoI RGCGCY 2 cut(s) 1131, 1243
BfuAI ACCTGC 1 cut(s) 1562
BfuI GTATCC 1 cut(s) 1655
BglI GCCNNNNNGGC 2 cut(s) 827, 1076
BglII AGATCT 2 cut(s) 917, 1444
BisI GCNGC 8 cut(s) 222, 437, 502, 549, 727, 1080, 1387, 1437
BlnI CCTAGG 1 cut(s) 707
BlsI GCNGC 8 cut(s) 223, 438, 503, 550, 728, 1081, 1388, 1438
BmcAI AGTACT 3 cut(s) 265, 493, 1610
Bme1390I CCNGG 3 cut(s) 538, 1626, 1659
Bme18I GGWCC 1 cut(s) 161
BmeT110I CYCGRG 2 cut(s) 348, 440
BmgT120I GGNCC 4 cut(s) 161, 613, 1133, 1622
BmiI GGNNCC 2 cut(s) 163, 1727
BmrFI CCNGG 3 cut(s) 538, 1626, 1659
BmsI GCATC 4 cut(s) 426, 1184, 1352, 1448
BoxI GACNNNNGTC 1 cut(s) 761
BpiI GAAGAC 1 cut(s) 1019
BpmI CTGGAG 2 cut(s) 930, 1437
BpuEI CTTGAG 4 cut(s) 452, 605, 661, 1677
Bsa29I ATCGAT 1 cut(s) 453
BsaAI YACGTR 1 cut(s) 931
BsaI GGTCTC 2 cut(s) 769, 1566
BsaJI CCNNGG 6 cut(s) 22, 707, 822, 1490, 1565, 1625
BsaXI ACNNNNNCTCC 2 cut(s) 1217, 1247
Bsc4I CCNNNNNNNGG 2 cut(s) 1571, 1745
Bse118I RCCGGY 2 cut(s) 1130, 1745
Bse1I ACTGG 5 cut(s) 882, 913, 1063, 1243, 1454
BseBI CCWGG 3 cut(s) 538, 1626, 1659
BseCI ATCGAT 1 cut(s) 453
BseDI CCNNGG 6 cut(s) 22, 707, 822, 1490, 1565, 1625
BseGI GGATG 6 cut(s) 131, 251, 751, 808, 841, 1540
BseLI CCNNNNNNNGG 2 cut(s) 1571, 1745
BseMII CTCAG 4 cut(s) 327, 556, 780, 1311
BseNI ACTGG 5 cut(s) 882, 913, 1063, 1243, 1454
BseRI GAGGAG 4 cut(s) 171, 201, 235, 908
BseX3I CGGCCG 1 cut(s) 727
BseXI GCAGC 7 cut(s) 233, 423, 513, 535, 1066, 1398, 1423
BseYI CCCAGC 2 cut(s) 938, 1553
BsgI GTGCAG 1 cut(s) 1191
Bsh1236I CGCG 1 cut(s) 1107
Bsh1285I CGRYCG 1 cut(s) 730
BshVI ATCGAT 1 cut(s) 453
BsiEI CGRYCG 1 cut(s) 730
BsiHKCI CYCGRG 2 cut(s) 348, 440
BsiSI CCGG 3 cut(s) 730, 1131, 1746
BslFI GGGAC 1 cut(s) 955
BslI CCNNNNNNNGG 2 cut(s) 1571, 1745
BsmAI GTCTC 4 cut(s) 617, 769, 1466, 1566
BsmFI GGGAC 1 cut(s) 955
Bso31I GGTCTC 2 cut(s) 769, 1566
BsoBI CYCGRG 2 cut(s) 348, 440
Bsp1286I GDGCHC 1 cut(s) 355
Bsp143I GATC 4 cut(s) 917, 1185, 1393, 1444
Bsp19I CCATGG 2 cut(s) 22, 1490
BspACI CCGC 1 cut(s) 726
BspCNI CTCAG 4 cut(s) 326, 555, 779, 1310
BspDI ATCGAT 1 cut(s) 453
BspFNI CGCG 1 cut(s) 1107
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 163, 1727
BspMI ACCTGC 1 cut(s) 1562
BspQI GCTCTTC 1 cut(s) 1164
BspTNI GGTCTC 2 cut(s) 769, 1566
BsrFI RCCGGY 2 cut(s) 1130, 1745
BsrI ACTGG 5 cut(s) 882, 913, 1063, 1243, 1454
BssAI RCCGGY 2 cut(s) 1130, 1745
BssECI CCNNGG 6 cut(s) 22, 707, 822, 1490, 1565, 1625
BssMI GATC 4 cut(s) 917, 1185, 1393, 1444
BssT1I CCWWGG 4 cut(s) 22, 707, 1490, 1565
Bst2UI CCWGG 3 cut(s) 538, 1626, 1659
Bst4CI ACNGT 6 cut(s) 449, 1075, 1276, 1513, 1687, 1705
Bst6I CTCTTC 1 cut(s) 1164
BstBAI YACGTR 1 cut(s) 931
BstC8I GCNNGC 5 cut(s) 479, 848, 1105, 1132, 1341
BstDEI CTNAG 7 cut(s) 313, 495, 542, 600, 633, 766, 1297
BstDSI CCRYGG 3 cut(s) 22, 822, 1490
BstENI CCTNNNNNAGG 1 cut(s) 1569
BstF5I GGATG 6 cut(s) 131, 251, 751, 808, 841, 1540
BstFNI CGCG 1 cut(s) 1107
BstH2I RGCGCY 2 cut(s) 1131, 1243
BstHHI GCGC 2 cut(s) 1130, 1242
BstKTI GATC 4 cut(s) 920, 1188, 1396, 1447
BstMAI GTCTC 4 cut(s) 617, 769, 1466, 1566
BstMBI GATC 4 cut(s) 917, 1185, 1393, 1444
BstMCI CGRYCG 1 cut(s) 730
BstMWI GCNNNNNNNGC 8 cut(s) 119, 344, 510, 827, 1076, 1136, 1386, 1495
BstNI CCWGG 3 cut(s) 538, 1626, 1659
BstNSI RCATGY 1 cut(s) 821
BstPAI GACNNNNGTC 1 cut(s) 761
BstSCI CCNGG 3 cut(s) 536, 1624, 1657
BstSFI CTRYAG 2 cut(s) 1249, 1686
BstUI CGCG 1 cut(s) 1107
BstV1I GCAGC 7 cut(s) 233, 423, 513, 535, 1066, 1398, 1423
BstV2I GAAGAC 1 cut(s) 1019
BstX2I RGATCY 2 cut(s) 917, 1444
BstXI CCANNNNNNTGG 3 cut(s) 410, 1024, 1268
BstYI RGATCY 2 cut(s) 917, 1444
BstZI CGGCCG 1 cut(s) 727
Bsu15I ATCGAT 1 cut(s) 453
BsuI GTATCC 1 cut(s) 1655
BsuTUI ATCGAT 1 cut(s) 453
BtgI CCRYGG 3 cut(s) 22, 822, 1490
BtsCI GGATG 6 cut(s) 131, 251, 751, 808, 841, 1540
BtsIMutI CAGTG 4 cut(s) 550, 1071, 1188, 1658
BveI ACCTGC 1 cut(s) 1562
Cac8I GCNNGC 5 cut(s) 479, 848, 1105, 1132, 1341
CciI TCATGA 1 cut(s) 675
CfoI GCGC 2 cut(s) 1130, 1242
Cfr10I RCCGGY 2 cut(s) 1130, 1745
Cfr13I GGNCC 4 cut(s) 161, 613, 1133, 1622
ClaI ATCGAT 1 cut(s) 453
Csp6I GTAC 4 cut(s) 264, 492, 903, 1609
CviAII CATG 8 cut(s) 23, 515, 619, 676, 759, 818, 1491, 1667
CviQI GTAC 4 cut(s) 264, 492, 903, 1609
DdeI CTNAG 7 cut(s) 313, 495, 542, 600, 633, 766, 1297
DpnI GATC 4 cut(s) 919, 1187, 1395, 1446
DpnII GATC 4 cut(s) 917, 1185, 1393, 1444
DrdI GACNNNNNNGTC 1 cut(s) 527
DseDI GACNNNNNNGTC 1 cut(s) 527
EaeI YGGCCR 3 cut(s) 511, 727, 1351
EagI CGGCCG 1 cut(s) 727
Eam1104I CTCTTC 1 cut(s) 1164
EarI CTCTTC 1 cut(s) 1164
EclXI CGGCCG 1 cut(s) 727
Eco130I CCWWGG 4 cut(s) 22, 707, 1490, 1565
Eco24I GRGCYC 1 cut(s) 355
Eco31I GGTCTC 2 cut(s) 769, 1566
Eco47I GGWCC 1 cut(s) 161
Eco52I CGGCCG 1 cut(s) 727
Eco57I CTGAAG 3 cut(s) 612, 1174, 1623
Eco88I CYCGRG 2 cut(s) 348, 440
EcoNI CCTNNNNNAGG 1 cut(s) 1569
EcoO109I RGGNCCY 2 cut(s) 161, 613
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 3 cut(s) 536, 1624, 1657
EcoT14I CCWWGG 4 cut(s) 22, 707, 1490, 1565
EcoT38I GRGCYC 1 cut(s) 355
ErhI CCWWGG 4 cut(s) 22, 707, 1490, 1565
FaeI CATG 8 cut(s) 26, 518, 622, 679, 762, 821, 1494, 1670
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FaqI GGGAC 1 cut(s) 955
FatI CATG 8 cut(s) 22, 514, 618, 675, 758, 817, 1490, 1666
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 8 cut(s) 222, 437, 502, 549, 727, 1080, 1387, 1437
FokI GGATG 6 cut(s) 118, 238, 758, 815, 848, 1547
FriOI GRGCYC 1 cut(s) 355
Fsp4HI GCNGC 8 cut(s) 222, 437, 502, 549, 727, 1080, 1387, 1437
FspBI CTAG 6 cut(s) 153, 575, 684, 708, 788, 1506
GlaI GCGC 2 cut(s) 1129, 1241
GluI GCNGC 8 cut(s) 222, 437, 502, 549, 727, 1080, 1387, 1437
GsaI CCCAGC 2 cut(s) 942, 1557
GsuI CTGGAG 2 cut(s) 930, 1437
HaeII RGCGCY 2 cut(s) 1131, 1243
HapII CCGG 3 cut(s) 730, 1131, 1746
HhaI GCGC 2 cut(s) 1130, 1242
Hin1II CATG 8 cut(s) 26, 518, 622, 679, 762, 821, 1494, 1670
Hin6I GCGC 2 cut(s) 1128, 1240
HinP1I GCGC 2 cut(s) 1128, 1240
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 3 cut(s) 97, 120, 782
HinfI GANTC 4 cut(s) 230, 455, 1538, 1760
HpaII CCGG 3 cut(s) 730, 1131, 1746
HphI GGTGA 7 cut(s) 120, 206, 386, 875, 1010, 1100, 1616
Hpy166II GTNNAC 3 cut(s) 310, 520, 607
Hpy188I TCNGA 6 cut(s) 745, 756, 1042, 1193, 1292, 1444
Hpy188III TCNNGA 8 cut(s) 70, 153, 563, 575, 676, 788, 1506, 1764
Hpy8I GTNNAC 3 cut(s) 310, 520, 607
HpyAV CCTTC 2 cut(s) 785, 1749
HpyCH4III ACNGT 6 cut(s) 449, 1075, 1276, 1513, 1687, 1705
HpyCH4IV ACGT 2 cut(s) 930, 984
HpyCH4V TGCA 9 cut(s) 201, 1139, 1172, 1320, 1331, 1343, 1425, 1439, 1522
HpyF10VI GCNNNNNNNGC 8 cut(s) 119, 344, 510, 827, 1076, 1136, 1386, 1495
HpyF3I CTNAG 7 cut(s) 313, 495, 542, 600, 633, 766, 1297
HpySE526I ACGT 2 cut(s) 930, 984
Hsp92II CATG 8 cut(s) 26, 518, 622, 679, 762, 821, 1494, 1670
HspAI GCGC 2 cut(s) 1128, 1240
KroI GCCGGC 1 cut(s) 1130
KroNI GCCGGC 1 cut(s) 1132
Kzo9I GATC 4 cut(s) 917, 1185, 1393, 1444
LguI GCTCTTC 1 cut(s) 1164
LmnI GCTCC 2 cut(s) 1202, 1456
Lsp1109I GCAGC 7 cut(s) 233, 423, 513, 535, 1066, 1398, 1423
LweI GCATC 4 cut(s) 426, 1184, 1352, 1448
MaeI CTAG 6 cut(s) 153, 575, 684, 708, 788, 1506
MaeII ACGT 2 cut(s) 930, 984
MaeIII GTNAC 2 cut(s) 1088, 1366
MalI GATC 4 cut(s) 919, 1187, 1395, 1446
MboI GATC 4 cut(s) 917, 1185, 1393, 1444
MboII GAAGA 5 cut(s) 117, 991, 1024, 1151, 1180
MfeI CAATTG 1 cut(s) 1116
MflI RGATCY 2 cut(s) 917, 1444
MhlI GDGCHC 1 cut(s) 355
MlsI TGGCCA 2 cut(s) 513, 1353
MluNI TGGCCA 2 cut(s) 513, 1353
MlyI GAGTC 1 cut(s) 1547
MmeI TCCRAC 3 cut(s) 723, 779, 1031
Mox20I TGGCCA 2 cut(s) 513, 1353
MroNI GCCGGC 1 cut(s) 1130
MscI TGGCCA 2 cut(s) 513, 1353
MseI TTAA 1 cut(s) 992
MslI CAYNNNNRTG 2 cut(s) 1022, 1748
Msp20I TGGCCA 2 cut(s) 513, 1353
MspA1I CMGCKG 2 cut(s) 1301, 1436
MspI CCGG 3 cut(s) 730, 1131, 1746
MspR9I CCNGG 3 cut(s) 538, 1626, 1659
MunI CAATTG 1 cut(s) 1116
MvaI CCWGG 3 cut(s) 538, 1626, 1659
MvnI CGCG 1 cut(s) 1107
MwoI GCNNNNNNNGC 8 cut(s) 119, 344, 510, 827, 1076, 1136, 1386, 1495
NaeI GCCGGC 1 cut(s) 1132
NcoI CCATGG 2 cut(s) 22, 1490
NdeII GATC 4 cut(s) 917, 1185, 1393, 1444
NgoMIV GCCGGC 1 cut(s) 1130
NlaIII CATG 8 cut(s) 26, 518, 622, 679, 762, 821, 1494, 1670
NlaIV GGNNCC 2 cut(s) 163, 1727
NmuCI GTSAC 2 cut(s) 1088, 1366
NspI RCATGY 1 cut(s) 821
OliI CACNNNNGTG 1 cut(s) 1748
PaeR7I CTCGAG 2 cut(s) 348, 440
PagI TCATGA 1 cut(s) 675
PaqCI CACCTGC 1 cut(s) 1562
PciSI GCTCTTC 1 cut(s) 1164
PcsI WCGNNNNNNNCGW 1 cut(s) 459
PdiI GCCGGC 1 cut(s) 1132
PfeI GAWTC 3 cut(s) 230, 455, 1760
PkrI GCNGC 8 cut(s) 223, 438, 503, 550, 728, 1081, 1388, 1438
PleI GAGTC 1 cut(s) 1546
PpsI GAGTC 1 cut(s) 1546
Ppu21I YACGTR 1 cut(s) 931
PpuMI RGGWCCY 1 cut(s) 161
PshAI GACNNNNGTC 1 cut(s) 761
PsiI TTATAA 1 cut(s) 273
Psp1406I AACGTT 1 cut(s) 984
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 3 cut(s) 536, 1624, 1657
PspFI CCCAGC 2 cut(s) 938, 1553
PspGI CCWGG 3 cut(s) 536, 1624, 1657
PspN4I GGNNCC 2 cut(s) 163, 1727
PspPI GGNCC 4 cut(s) 161, 613, 1133, 1622
PspPPI RGGWCCY 1 cut(s) 161
PspXI VCTCGAGB 2 cut(s) 348, 440
PsuI RGATCY 2 cut(s) 917, 1444
PvuII CAGCTG 2 cut(s) 1301, 1436
RsaI GTAC 4 cut(s) 265, 493, 904, 1610
RsaNI GTAC 4 cut(s) 264, 492, 903, 1609
RseI CAYNNNNRTG 2 cut(s) 1022, 1748
SapI GCTCTTC 1 cut(s) 1164
SaqAI TTAA 1 cut(s) 992
SatI GCNGC 8 cut(s) 222, 437, 502, 549, 727, 1080, 1387, 1437
Sau3AI GATC 4 cut(s) 917, 1185, 1393, 1444
Sau96I GGNCC 4 cut(s) 161, 613, 1133, 1622
ScaI AGTACT 3 cut(s) 265, 493, 1610
SchI GAGTC 1 cut(s) 1547
ScrFI CCNGG 3 cut(s) 538, 1626, 1659
SduI GDGCHC 1 cut(s) 355
SfaNI GCATC 4 cut(s) 426, 1184, 1352, 1448
SfcI CTRYAG 2 cut(s) 1249, 1686
Sfr274I CTCGAG 2 cut(s) 348, 440
SgrAI CRCCGGYG 1 cut(s) 1745
SinI GGWCC 1 cut(s) 161
SlaI CTCGAG 2 cut(s) 348, 440
SmiMI CAYNNNNRTG 2 cut(s) 1022, 1748
SmlI CTYRAG 6 cut(s) 348, 431, 440, 584, 640, 1692
SmoI CTYRAG 6 cut(s) 348, 431, 440, 584, 640, 1692
SpeI ACTAGT 1 cut(s) 683
SsiI CCGC 1 cut(s) 726
SspI AATATT 1 cut(s) 187
SspMI CTAG 6 cut(s) 153, 575, 684, 708, 788, 1506
StyD4I CCNGG 3 cut(s) 536, 1624, 1657
StyI CCWWGG 4 cut(s) 22, 707, 1490, 1565
TaaI ACNGT 6 cut(s) 449, 1075, 1276, 1513, 1687, 1705
TaiI ACGT 2 cut(s) 933, 987
TaqI TCGA 8 cut(s) 318, 349, 441, 453, 462, 1162, 1278, 1758
TatI WGTACW 3 cut(s) 263, 491, 1608
TauI GCSGC 1 cut(s) 729
TfiI GAWTC 3 cut(s) 230, 455, 1760
Tru1I TTAA 1 cut(s) 992
Tru9I TTAA 1 cut(s) 992
TscAI CASTG 4 cut(s) 550, 1078, 1188, 1658
TseFI GTSAC 2 cut(s) 1088, 1366
TseI GCWGC 7 cut(s) 221, 436, 501, 548, 1079, 1386, 1436
Tsp45I GTSAC 2 cut(s) 1088, 1366
TspDTI ATGAA 7 cut(s) 229, 640, 664, 819, 966, 1273, 1705
TspGWI ACGGA 2 cut(s) 788, 1107
TspRI CASTG 4 cut(s) 550, 1078, 1188, 1658
VpaK11BI GGWCC 1 cut(s) 161
XagI CCTNNNNNAGG 1 cut(s) 1569
XapI RAATTY 4 cut(s) 81, 146, 320, 1615
XbaI TCTAGA 4 cut(s) 152, 574, 787, 1505
XceI RCATGY 1 cut(s) 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XhoI CTCGAG 2 cut(s) 348, 440
XmaJI CCTAGG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 6 cut(s) 153, 575, 684, 708, 788, 1506
ZrmI AGTACT 3 cut(s) 265, 493, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.