AT2G44420

protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005345
Physical Location & Seq
Forward (+)
18330447 .. 18332936
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44420.3

Sequence Viewer

Length: 1044 bp
ATGATCTACGTTGGTGGAGTGCAATTTCTAGACGAGAGTAGCTCTTTTTCATTGTCTTCTTCATCGCAGGGAAGTAGCTTGCTTGTTGATGTAATGAGTCATCCTGTGATAACGTTAGCTTCTGATTCCTTTAAGAATCTTGAGGAAAAGAATGTTTCTTTCGATGAAAGTGATTCAGAGAGCTCAACAAAAGACAGATATGTTTACATATTCCAAAGAGAATTTGCTGTTGTGAATCCAGCTCTTGTTGATTTTGTTGGCACCGATGAAGCAACAACTTGTGTAGGCCTTGTTATCCGCAACAGAAAATCTGGAATGACGTCTGTTGCACACATGGATTCGCCAGAGATTGTGGATTTGGGGATAAGCCAGATGTTGTTATTGGTTTTACAGGATGACGTAGATGCCGAGTTAGATGTACATATGGTTGGTGGTTATGAAGATGTCGACATAAAAAATGCTGATGGTGTTGGTGACTATGCCAAACCAGAGGGTTATTCATTTCCGTTGTGTTGCAAATTAGTAGAAACTTTGCAGAAGAGAAGAGAAAACTTTCACATTCAAACCCTCTTTATTCTTGGGCACAATACCAAGTTGGATTCACAAGCAAACACGTGCCCCATTTTTAATGGATGCCTGGTGAATACCAGCACCGGTGCAATCTTGCCAGCCAGTTTCAACAGAACATCTAGATGTCCGGATGAGATCGTTAGAAGAATTCGAGTTTCATCATCGTTTGAAGATTCTAGCTGGAAGGGAAAGTTACTTGACACATATGACACAAAAACTGATAGATTCATCATCGCACCATGCCGCTGGACAATGCGCTTGATTGAATATGTTTGGGAACTTAATCAGCTTACTGATGAAGAAATCCTCACTAACTGTTCCACCTCACCTTCTGCTGAAGGTCCAGACTTCGTAAACAGTTTAAGAAGGAATTGGGGATATTTACTGAAATATCCAGAATGGAGCAAAACATTCCCAAGGAGGCAACCACGTGTGTTTGAAAGAACTGTCGATGGACACTGGAAAAAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

347

Amino Acids

39.17

Weight (kDa)

5.0

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 44 - 331 1e-92 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 323
AccB1I GGYRCC 1 cut(s) 260
AccI GTMKAC 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 697
AciI CCGC 2 cut(s) 298, 814
AclI AACGTT 1 cut(s) 113
AcsI RAATTY 2 cut(s) 221, 717
AcuI CTGAAG 1 cut(s) 927
AcvI CACGTG 2 cut(s) 615, 1001
AcyI GRCGYC 1 cut(s) 320
AfaI GTAC 1 cut(s) 420
AflIII ACRYGT 2 cut(s) 612, 1000
AgeI ACCGGT 1 cut(s) 653
AgsI TTSAA 5 cut(s) 563, 679, 740, 836, 1010
AjnI CCWGG 1 cut(s) 636
AluBI AGCT 7 cut(s) 42, 78, 119, 183, 242, 750, 859
AluI AGCT 7 cut(s) 42, 78, 119, 183, 242, 750, 859
Alw21I GWGCWC 1 cut(s) 185
Aor13HI TCCGGA 1 cut(s) 697
AoxI GGCC 1 cut(s) 286
ApoI RAATTY 2 cut(s) 221, 717
AsiGI ACCGGT 1 cut(s) 653
Asp700I GAANNNNTTC 1 cut(s) 552
AspLEI GCGC 1 cut(s) 828
AspS9I GGNCC 1 cut(s) 911
AsuHPI GGTGA 3 cut(s) 485, 652, 888
AvaII GGWCC 1 cut(s) 911
BaeGI GKGCMC 2 cut(s) 585, 620
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 1 cut(s) 185
BbrPI CACGTG 2 cut(s) 615, 1001
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 185
BccI CCATC 2 cut(s) 458, 1016
BciT130I CCWGG 1 cut(s) 638
BfaI CTAG 3 cut(s) 29, 690, 747
BisI GCNGC 1 cut(s) 814
BlsI GCNGC 1 cut(s) 815
Bme1390I CCNGG 1 cut(s) 638
Bme18I GGWCC 1 cut(s) 911
BmgT120I GGNCC 1 cut(s) 911
BmiI GGNNCC 1 cut(s) 262
BmrFI CCNGG 1 cut(s) 638
BmsI GCATC 2 cut(s) 394, 623
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 26, 58
BpuEI CTTGAG 1 cut(s) 161
BsaAI YACGTR 2 cut(s) 615, 1001
BsaHI GRCGYC 1 cut(s) 320
BsaJI CCNNGG 1 cut(s) 986
BsaWI WCCGGW 2 cut(s) 653, 697
Bse118I RCCGGY 1 cut(s) 653
Bse1I ACTGG 2 cut(s) 672, 1034
BseAI TCCGGA 1 cut(s) 697
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 1 cut(s) 986
BseGI GGATG 4 cut(s) 100, 400, 638, 706
BseNI ACTGG 2 cut(s) 672, 1034
BseSI GKGCMC 2 cut(s) 585, 620
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 260
BshTI ACCGGT 1 cut(s) 653
BsiHKAI GWGCWC 1 cut(s) 185
BsiSI CCGG 2 cut(s) 654, 698
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 3 cut(s) 185, 585, 620
Bsp13I TCCGGA 1 cut(s) 697
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 2 cut(s) 3, 705
BspACI CCGC 2 cut(s) 298, 814
BspANI GGCC 1 cut(s) 288
BspEI TCCGGA 1 cut(s) 697
BspLI GGNNCC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 260
BsrFI RCCGGY 1 cut(s) 653
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 2 cut(s) 672, 1034
BssAI RCCGGY 1 cut(s) 653
BssECI CCNNGG 1 cut(s) 986
BssMI GATC 2 cut(s) 3, 705
BssNI GRCGYC 1 cut(s) 320
BssT1I CCWWGG 1 cut(s) 986
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 3 cut(s) 887, 929, 1018
Bst6I CTCTTC 2 cut(s) 533, 538
BstACI GRCGYC 1 cut(s) 320
BstAUI TGTACA 1 cut(s) 418
BstBAI YACGTR 2 cut(s) 615, 1001
BstC8I GCNNGC 2 cut(s) 80, 669
BstF5I GGATG 4 cut(s) 100, 400, 638, 706
BstHHI GCGC 1 cut(s) 828
BstKTI GATC 2 cut(s) 6, 708
BstMBI GATC 2 cut(s) 3, 705
BstNI CCWGG 1 cut(s) 638
BstSCI CCNGG 1 cut(s) 636
BstSLI GKGCMC 2 cut(s) 585, 620
BstV2I GAAGAC 1 cut(s) 48
BstXI CCANNNNNNTGG 1 cut(s) 816
BsuRI GGCC 1 cut(s) 288
BtgZI GCGATG 2 cut(s) 48, 787
BtsCI GGATG 4 cut(s) 100, 400, 638, 706
BtsIMutI CAGTG 1 cut(s) 1027
Cac8I GCNNGC 2 cut(s) 80, 669
CfoI GCGC 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 911
Csp6I GTAC 1 cut(s) 419
CspAI ACCGGT 1 cut(s) 653
CviAII CATG 2 cut(s) 334, 810
CviQI GTAC 1 cut(s) 419
DpnI GATC 2 cut(s) 5, 707
DpnII GATC 2 cut(s) 3, 705
Eam1104I CTCTTC 2 cut(s) 533, 538
EarI CTCTTC 2 cut(s) 533, 538
Ecl136II GAGCTC 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 986
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 911
Eco53kI GAGCTC 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 927
Eco72I CACGTG 2 cut(s) 615, 1001
EcoICRI GAGCTC 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 636
EcoT14I CCWWGG 1 cut(s) 986
EcoT38I GRGCYC 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 986
FaeI CATG 2 cut(s) 337, 813
FatI CATG 2 cut(s) 333, 809
FauNDI CATATG 2 cut(s) 423, 775
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 814
FokI GGATG 4 cut(s) 87, 407, 645, 713
FriOI GRGCYC 1 cut(s) 185
Fsp4HI GCNGC 1 cut(s) 814
FspBI CTAG 3 cut(s) 29, 690, 747
GlaI GCGC 1 cut(s) 827
GluI GCNGC 1 cut(s) 814
HaeIII GGCC 1 cut(s) 288
HapII CCGG 2 cut(s) 654, 698
HhaI GCGC 1 cut(s) 828
Hin1I GRCGYC 1 cut(s) 320
Hin1II CATG 2 cut(s) 337, 813
Hin6I GCGC 1 cut(s) 826
HinP1I GCGC 1 cut(s) 826
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HinfI GANTC 9 cut(s) 97, 125, 136, 173, 235, 338, 599, 743, 795
HpaII CCGG 2 cut(s) 654, 698
HphI GGTGA 3 cut(s) 485, 652, 888
Hpy166II GTNNAC 3 cut(s) 205, 448, 925
Hpy188I TCNGA 2 cut(s) 124, 178
Hpy188III TCNNGA 7 cut(s) 29, 140, 312, 690, 698, 914, 965
Hpy8I GTNNAC 3 cut(s) 205, 448, 925
HpyAV CCTTC 4 cut(s) 748, 902, 909, 930
HpyCH4III ACNGT 3 cut(s) 887, 929, 1018
HpyCH4IV ACGT 6 cut(s) 9, 113, 320, 399, 614, 1000
HpyCH4V TGCA 5 cut(s) 22, 329, 516, 535, 659
HpySE526I ACGT 6 cut(s) 9, 113, 320, 399, 614, 1000
Hsp92I GRCGYC 1 cut(s) 320
Hsp92II CATG 2 cut(s) 337, 813
HspAI GCGC 1 cut(s) 826
Kpn2I TCCGGA 1 cut(s) 697
Kzo9I GATC 2 cut(s) 3, 705
LmnI GCTCC 1 cut(s) 972
LweI GCATC 2 cut(s) 394, 623
MaeI CTAG 3 cut(s) 29, 690, 747
MaeII ACGT 6 cut(s) 9, 113, 320, 399, 614, 1000
MaeIII GTNAC 2 cut(s) 473, 762
MalI GATC 2 cut(s) 5, 707
MboI GATC 2 cut(s) 3, 705
MboII GAAGA 8 cut(s) 48, 51, 452, 550, 555, 726, 752, 881
MhlI GDGCHC 3 cut(s) 185, 585, 620
MluCI AATT 5 cut(s) 23, 221, 518, 717, 940
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 1 cut(s) 576
MnlI CCTC 6 cut(s) 136, 484, 578, 887, 904, 984
MroI TCCGGA 1 cut(s) 697
MroXI GAANNNNTTC 1 cut(s) 552
MseI TTAA 4 cut(s) 132, 627, 852, 932
MslI CAYNNNNRTG 1 cut(s) 691
MspA1I CMGCKG 1 cut(s) 816
MspI CCGG 2 cut(s) 654, 698
MspR9I CCNGG 1 cut(s) 638
MvaI CCWGG 1 cut(s) 638
NdeI CATATG 2 cut(s) 423, 775
NdeII GATC 2 cut(s) 3, 705
NlaIII CATG 2 cut(s) 337, 813
NlaIV GGNNCC 1 cut(s) 262
NmeAIII GCCGAG 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 473
PceI AGGCCT 1 cut(s) 288
PcsI WCGNNNNNNNCGW 1 cut(s) 405
PdmI GAANNNNTTC 1 cut(s) 552
PfeI GAWTC 8 cut(s) 125, 136, 173, 235, 338, 599, 743, 795
PinAI ACCGGT 1 cut(s) 653
PkrI GCNGC 1 cut(s) 815
PleI GAGTC 1 cut(s) 105
PmaCI CACGTG 2 cut(s) 615, 1001
PmlI CACGTG 2 cut(s) 615, 1001
PpsI GAGTC 1 cut(s) 105
Ppu21I YACGTR 2 cut(s) 615, 1001
Psp124BI GAGCTC 1 cut(s) 185
Psp1406I AACGTT 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 636
PspCI CACGTG 2 cut(s) 615, 1001
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 1 cut(s) 262
PspPI GGNCC 1 cut(s) 911
RsaI GTAC 1 cut(s) 420
RsaNI GTAC 1 cut(s) 419
RseI CAYNNNNRTG 1 cut(s) 691
SacI GAGCTC 1 cut(s) 185
SalI GTCGAC 1 cut(s) 446
SaqAI TTAA 4 cut(s) 132, 627, 852, 932
SatI GCNGC 1 cut(s) 814
Sau3AI GATC 2 cut(s) 3, 705
Sau96I GGNCC 1 cut(s) 911
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 1 cut(s) 638
SduI GDGCHC 3 cut(s) 185, 585, 620
SfaNI GCATC 2 cut(s) 394, 623
SgrAI CRCCGGYG 1 cut(s) 653
SinI GGWCC 1 cut(s) 911
SmiMI CAYNNNNRTG 1 cut(s) 691
SmlI CTYRAG 1 cut(s) 140
SmoI CTYRAG 1 cut(s) 140
Sse9I AATT 5 cut(s) 23, 221, 518, 717, 940
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 2 cut(s) 298, 814
SspMI CTAG 3 cut(s) 29, 690, 747
SstI GAGCTC 1 cut(s) 185
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 636
StyI CCWWGG 1 cut(s) 986
TaaI ACNGT 3 cut(s) 887, 929, 1018
TaiI ACGT 6 cut(s) 12, 116, 323, 402, 617, 1003
TaqI TCGA 4 cut(s) 162, 447, 721, 1020
TasI AATT 5 cut(s) 23, 221, 518, 717, 940
TatI WGTACW 1 cut(s) 418
TauI GCSGC 1 cut(s) 816
TfiI GAWTC 8 cut(s) 125, 136, 173, 235, 338, 599, 743, 795
Tru1I TTAA 4 cut(s) 132, 627, 852, 932
Tru9I TTAA 4 cut(s) 132, 627, 852, 932
TscAI CASTG 1 cut(s) 1034
TseFI GTSAC 1 cut(s) 473
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 9 cut(s) 39, 51, 180, 282, 453, 489, 717, 787, 882
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 911
XapI RAATTY 2 cut(s) 221, 717
XbaI TCTAGA 2 cut(s) 28, 689
XmiI GTMKAC 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 552
XspI CTAG 3 cut(s) 29, 690, 747
ZraI GACGTC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.