Rroxscaffold_4G00332200

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005345
Physical Location & Seq
Reverse (-)
66235031 .. 66238210
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00332200.1

Sequence Viewer

Length: 1041 bp
ATGATCTTTGTAGGTGGACTTCCTTTCCCTTCCGAATCATCTTCATCGCCTCAGCCTCAGGGATGGGACACCCTCCAAGCTTTGTTGGATCACCCGTTTTTGGAATCTGCCTCCACTACATTCAAGGCCATCCCGGAGAGGAAGTTCTCGGTGCCCCAAGAATCAGAATCAGAGAGGAGATGGGTTTATCTCTTCCAAAGGGAATATGCAACTGTTGATCCTGCATATGTCGATTTTGTTGGCACTGATGAAGCAACAACCTGTATGGGTCTTGCCATCCGTAACCCAGAAACTGGAATGACGTCAGTTGCTCATTTAGACTCTCCAAAAGTTGTGGATATGGGTCTCTCCCAGATGGTGTCACTACTAGTTCAACCCAATGAGGACGTGGAGTTGGATGTGCATCTAGTTGGTGGTTTTGAAGATGTTTCACCTAAACATGGTAACTGTAATACTAGATCAGAAAATCAGGCAAACTTGGATGGTTATTCCTTTCCTTTAGCTGCCAAAATTGTTGAAACATTATGCAAGAGCCGGGAGAGATTTCATATTCGAACTCTATGCATTCTTCGGCATAACACCAGAAGGGATCCCGAGGGAAATGCATACCCAATTTTCAACGGGTTCATGGTAAGCACTTCCACGGGATCTATTATTCCTGCCAGCTTTGACGAAACCTCAAGATGGCCTGATGAAATTGTCAGGAGAATTAGAGTTTCTGCATCTTATGAGGACTCCAGATGGAAAGGAAGGTTATTGGAAACATATGAAACTCAAACTGATCTTTTCAAAATAGCTCCATGCTTCTGGAGCCTACGTCAGTTGCATATTGCGTTGTCCCTACAAAATTATTCAGATTCAGAAATCCTCCTTATGTGTTCTACTTCACCCTCAGCTGAGGCACCGGATTTTGTGAAAAATCATAGAAGGGTGTGGGACTACTTGATTGAGTACCCAGATTGGAGAGAAACCTTTCCTATGGAGCAGCCACGCGTATTTCAGAGGACTGCTGAGGGAGGCTGGAAAACCAAAAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

346

Amino Acids

39.48

Weight (kDa)

5.3

Isoelectric Point (pI)

59.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 53 - 342 7.4e-85 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
AccB1I GGYRCC 2 cut(s) 151, 901
AccB7I CCANNNNNTGG 1 cut(s) 293
AccII CGCG 1 cut(s) 993
AclWI GGATC 5 cut(s) 96, 212, 584, 597, 655
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 4 cut(s) 100, 293, 440, 684
AflIII ACRYGT 1 cut(s) 991
AgsI TTSAA 6 cut(s) 124, 374, 422, 518, 619, 790
AhlI ACTAGT 1 cut(s) 367
AjiI CACGTC 1 cut(s) 388
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 5 cut(s) 80, 503, 666, 797, 896
AluI AGCT 5 cut(s) 80, 503, 666, 797, 896
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 5 cut(s) 96, 212, 584, 597, 655
AlwNI CAGNNNCTG 1 cut(s) 293
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 2 cut(s) 126, 686
ApeKI GCWGC 2 cut(s) 503, 985
Asp700I GAANNNNTTC 1 cut(s) 972
AsuC2I CCSGG 2 cut(s) 134, 536
AsuHPI GGTGA 3 cut(s) 83, 423, 879
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 156
BamHI GGATCC 1 cut(s) 589
BanI GGYRCC 2 cut(s) 151, 901
BarI GAAGNNNNNNTAC 1 cut(s) 35
BbvCI CCTCAGC 4 cut(s) 51, 892, 897, 1011
BbvI GCAGC 2 cut(s) 490, 997
BccI CCATC 8 cut(s) 57, 137, 174, 284, 349, 476, 678, 735
BcgI CGANNNNNNTGC 4 cut(s) 543, 577, 584, 618
BcnI CCSGG 2 cut(s) 134, 536
BcoDI GTCTC 1 cut(s) 350
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 3 cut(s) 368, 407, 456
BisI GCNGC 2 cut(s) 504, 986
BlsI GCNGC 2 cut(s) 505, 987
Bme1390I CCNGG 2 cut(s) 134, 536
BmeT110I CYCGRG 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 388
BmiI GGNNCC 4 cut(s) 153, 591, 812, 903
BmrFI CCNGG 2 cut(s) 134, 536
BmsI GCATC 2 cut(s) 412, 731
BpmI CTGGAG 2 cut(s) 721, 829
Bpu10I CCTNAGC 4 cut(s) 51, 892, 897, 1011
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 2 cut(s) 134, 536
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 594, 642
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 802, 832
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 293, 440, 684
Bse1I ACTGG 1 cut(s) 298
Bse21I CCTNAGG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 594, 642
BseGI GGATG 5 cut(s) 68, 129, 276, 403, 487
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 100, 293, 440, 684
BseMII CTCAG 5 cut(s) 65, 71, 888, 906, 1002
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 2 cut(s) 490, 997
Bsh1236I CGCG 1 cut(s) 993
BshFI GGCC 2 cut(s) 128, 688
BshNI GGYRCC 2 cut(s) 151, 901
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 3 cut(s) 134, 535, 905
BslFI GGGAC 3 cut(s) 80, 823, 950
BslI CCNNNNNNNGG 4 cut(s) 100, 293, 440, 684
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 3 cut(s) 80, 823, 950
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 2 cut(s) 128, 688
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BspANI GGCC 2 cut(s) 128, 688
BspCNI CTCAG 5 cut(s) 64, 70, 889, 905, 1003
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 4 cut(s) 153, 591, 812, 903
BspPI GGATC 5 cut(s) 96, 212, 584, 597, 655
BspT104I TTCGAA 1 cut(s) 553
BspT107I GGYRCC 2 cut(s) 151, 901
BspTNI GGTCTC 1 cut(s) 350
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 594, 642
BssMI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BssNI GRCGYC 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 214, 449
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 5 cut(s) 51, 57, 892, 897, 1011
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 5 cut(s) 68, 129, 276, 403, 487
BstFNI CGCG 1 cut(s) 993
BstKTI GATC 7 cut(s) 6, 91, 220, 461, 592, 650, 784
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BstMWI GCNNNNNNNGC 1 cut(s) 810
BstSCI CCNGG 2 cut(s) 132, 534
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 2 cut(s) 490, 997
BstX2I RGATCY 2 cut(s) 589, 647
BstXI CCANNNNNNTGG 1 cut(s) 807
BstYI RGATCY 2 cut(s) 589, 647
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 2 cut(s) 128, 688
BtgI CCRYGG 1 cut(s) 642
BtgZI GCGATG 1 cut(s) 30
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 5 cut(s) 68, 129, 276, 403, 487
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 664
CaiI CAGNNNCTG 1 cut(s) 293
Csp6I GTAC 1 cut(s) 952
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 3 cut(s) 440, 628, 801
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 5 cut(s) 51, 57, 892, 897, 1011
DpnI GATC 7 cut(s) 5, 90, 219, 460, 591, 649, 783
DpnII GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 593
EcoT22I ATGCAT 2 cut(s) 566, 607
FaeI CATG 3 cut(s) 443, 631, 804
FaqI GGGAC 3 cut(s) 80, 823, 950
FatI CATG 3 cut(s) 439, 627, 800
FauNDI CATATG 2 cut(s) 226, 766
Fnu4HI GCNGC 2 cut(s) 504, 986
FokI GGATG 5 cut(s) 75, 116, 263, 410, 494
Fsp4HI GCNGC 2 cut(s) 504, 986
FspBI CTAG 3 cut(s) 368, 407, 456
GluI GCNGC 2 cut(s) 504, 986
GsuI CTGGAG 2 cut(s) 721, 829
HaeIII GGCC 2 cut(s) 128, 688
HapII CCGG 3 cut(s) 134, 535, 905
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 3 cut(s) 443, 631, 804
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 7 cut(s) 35, 104, 161, 167, 320, 734, 857
HpaII CCGG 3 cut(s) 134, 535, 905
HphI GGTGA 3 cut(s) 83, 423, 879
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 7 cut(s) 34, 166, 172, 463, 856, 862, 1002
Hpy188III TCNNGA 5 cut(s) 593, 681, 703, 738, 808
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 4 cut(s) 39, 579, 744, 921
HpyCH4III ACNGT 2 cut(s) 214, 449
HpyCH4IV ACGT 3 cut(s) 302, 387, 817
HpyCH4V TGCA 8 cut(s) 209, 224, 403, 528, 564, 605, 722, 826
HpyF10VI GCNNNNNNNGC 1 cut(s) 810
HpyF3I CTNAG 5 cut(s) 51, 57, 892, 897, 1011
HpySE526I ACGT 3 cut(s) 302, 387, 817
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 3 cut(s) 443, 631, 804
Kzo9I GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
LmnI GCTCC 3 cut(s) 802, 810, 982
Lsp1109I GCAGC 2 cut(s) 490, 997
LweI GCATC 2 cut(s) 412, 731
MaeI CTAG 3 cut(s) 368, 407, 456
MaeII ACGT 3 cut(s) 302, 387, 817
MaeIII GTNAC 3 cut(s) 281, 360, 443
MalI GATC 7 cut(s) 5, 90, 219, 460, 591, 649, 783
MboI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
MboII GAAGA 4 cut(s) 33, 184, 434, 560
MflI RGATCY 2 cut(s) 589, 647
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 5 cut(s) 510, 612, 696, 708, 847
MluI ACGCGT 1 cut(s) 991
MlyI GAGTC 2 cut(s) 314, 728
MmeI TCCRAC 2 cut(s) 66, 375
Mph1103I ATGCAT 2 cut(s) 566, 607
MroXI GAANNNNTTC 1 cut(s) 972
MspA1I CMGCKG 1 cut(s) 896
MspI CCGG 3 cut(s) 134, 535, 905
MspR9I CCNGG 2 cut(s) 134, 536
Mva1269I GAATGC 1 cut(s) 564
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 1 cut(s) 810
NciI CCSGG 2 cut(s) 134, 536
NdeI CATATG 2 cut(s) 226, 766
NdeII GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
NlaIII CATG 3 cut(s) 443, 631, 804
NlaIV GGNNCC 4 cut(s) 153, 591, 812, 903
NmuCI GTSAC 1 cut(s) 360
NsiI ATGCAT 2 cut(s) 566, 607
NspV TTCGAA 1 cut(s) 553
PctI GAATGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 5 cut(s) 35, 104, 161, 167, 857
PflMI CCANNNNNTGG 1 cut(s) 293
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 2 cut(s) 505, 987
PleI GAGTC 2 cut(s) 314, 728
PpsI GAGTC 2 cut(s) 314, 728
PspN4I GGNNCC 4 cut(s) 153, 591, 812, 903
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 2 cut(s) 589, 647
PvuII CAGCTG 1 cut(s) 896
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
SatI GCNGC 2 cut(s) 504, 986
Sau3AI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
SchI GAGTC 2 cut(s) 314, 728
ScrFI CCNGG 2 cut(s) 134, 536
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 2 cut(s) 412, 731
SfuI TTCGAA 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 5 cut(s) 510, 612, 696, 708, 847
SspMI CTAG 3 cut(s) 368, 407, 456
StyD4I CCNGG 2 cut(s) 132, 534
TaaI ACNGT 2 cut(s) 214, 449
TaiI ACGT 3 cut(s) 305, 390, 820
TaqI TCGA 2 cut(s) 231, 553
TasI AATT 5 cut(s) 510, 612, 696, 708, 847
TfiI GAWTC 5 cut(s) 35, 104, 161, 167, 857
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 2 cut(s) 503, 985
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 6 cut(s) 33, 264, 536, 616, 708, 783
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 293
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 3 cut(s) 368, 407, 456
ZraI GACGTC 1 cut(s) 303
Zsp2I ATGCAT 2 cut(s) 566, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.