RLG00000030798

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005345
Physical Location & Seq
Reverse (-)
67701806 .. 67704796
2991 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030798

Sequence Viewer

Length: 1041 bp
ATGATCTTTGTAGGTGGACGTCCTTTCCCTTCCGAATCATCTTCATCGCCTCAGCCTCAGGGATGGGACACCCTCCAAGCTTTGTTGGATCACCCATTTTTGGAATCTGCCTCCAATACATTCAAGGCCATCCCGGAGAGGAAGTTCTCGGTGCCCCAAGAATCAGAATCTGAGAGGAGATGGGTTTATCTCTTCCAAAGAGAATATGCAACTGTTGATCCTGCATATGTCGATTTTGTTGGCACTGATGAAGCAACAACCTGTATGGGTCTTGCCATCCGTAACCCGGAAACTGGAATGACGTCGGTTGCCCATTTAGACTCTCCAAAAGTTGTGGATAAGGGTCTCTCCCAGATGGTGTCACTACTAGTTCAACACAATAAGGACGTCGAGTTGGATGTGCATCTAGTTGGTGGTTTTGAAGATGTTTCACCTAATCATGGTAACTGTAATACTAGATCAGAAAATCAGGCAAAGTTGGATGGTTATTCCTTTCCTTTAGCTGCCAAAATTGTTGAAACATTATGCAAGAGCCGGGAGAGATTTCATATTCGAACTCTATGCATTCTTCGGCATAACACCAGAAGGGATCCCGAGGGAAATACATACCCAATTTTCAACGGGTTCATGGTAAGCACTTCCACGGGATCTATTATTCCTGCCAGCTTTGACGAAACCTCAAGATGTCCTGATGAAATTGTCAGGAGAATTAGAGTTTCTGCATCTTATGAGGACCCCAGATGGAAAGGAAGGTTATTGGAAACATATGAAACTCAAACTGATCTTTTCAAAATAGCTCCATGCTGCTGGAGCCTATATCAGTTGCATGTTGCGTTTTCCCTACAGAATTATTCAGATTCAGAAATCCTCCTTACGTGTTCTACTTCACCCTCTGCTGAGGCACCGGATTTTGTGAAAAATCAAAGAAGGGTGTGGGACTACTTGATTGAGTACCCAGATTGGAGAGAAACCTTTCCTATGGAGCAGCCACGCGTGTTTCAGAGGACTGCTGAGGGAGGCTGGAAAGCCAAAAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

347

Amino Acids

39.44

Weight (kDa)

5.59

Isoelectric Point (pI)

53.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 53 - 342 3e-87 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 22, 305, 390
AccB1I GGYRCC 2 cut(s) 151, 901
AccII CGCG 1 cut(s) 993
AclWI GGATC 5 cut(s) 96, 212, 584, 597, 655
AcyI GRCGYC 3 cut(s) 19, 302, 387
AfaI GTAC 1 cut(s) 953
AfiI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
AflIII ACRYGT 2 cut(s) 875, 991
AgsI TTSAA 6 cut(s) 124, 374, 422, 518, 619, 790
AhlI ACTAGT 1 cut(s) 367
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 4 cut(s) 80, 503, 666, 797
AluI AGCT 4 cut(s) 80, 503, 666, 797
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 5 cut(s) 96, 212, 584, 597, 655
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 3 cut(s) 503, 804, 985
Asp700I GAANNNNTTC 1 cut(s) 972
AspS9I GGNCC 1 cut(s) 733
AsuC2I CCSGG 3 cut(s) 134, 287, 536
AsuHPI GGTGA 3 cut(s) 83, 423, 879
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 1 cut(s) 593
AvaII GGWCC 1 cut(s) 733
AxyI CCTNAGG 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 156
BamHI GGATCC 1 cut(s) 589
BanI GGYRCC 2 cut(s) 151, 901
BbvCI CCTCAGC 3 cut(s) 51, 897, 1011
BbvI GCAGC 3 cut(s) 490, 791, 997
BccI CCATC 7 cut(s) 57, 137, 174, 284, 349, 476, 735
BcgI CGANNNNNNTGC 2 cut(s) 543, 577
BcnI CCSGG 3 cut(s) 134, 287, 536
BcoDI GTCTC 1 cut(s) 350
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 3 cut(s) 368, 407, 456
BfmI CTRYAG 1 cut(s) 842
BisI GCNGC 3 cut(s) 504, 805, 986
BlsI GCNGC 3 cut(s) 505, 806, 987
Bme1390I CCNGG 3 cut(s) 134, 287, 536
Bme18I GGWCC 1 cut(s) 733
BmeT110I CYCGRG 1 cut(s) 593
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 5 cut(s) 153, 591, 735, 812, 903
BmrFI CCNGG 3 cut(s) 134, 287, 536
BmsI GCATC 2 cut(s) 412, 731
BpmI CTGGAG 1 cut(s) 829
Bpu10I CCTNAGC 3 cut(s) 51, 897, 1011
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 3 cut(s) 134, 287, 536
BsaAI YACGTR 1 cut(s) 876
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 3 cut(s) 19, 302, 387
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 2 cut(s) 594, 642
BsaWI WCCGGW 1 cut(s) 904
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
Bse1I ACTGG 1 cut(s) 298
Bse21I CCTNAGG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 594, 642
BseGI GGATG 5 cut(s) 68, 129, 276, 403, 487
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
BseMII CTCAG 5 cut(s) 65, 71, 162, 888, 1002
BseNI ACTGG 1 cut(s) 298
BseRI GAGGAG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 3 cut(s) 490, 791, 997
Bsh1236I CGCG 1 cut(s) 993
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 2 cut(s) 151, 901
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 4 cut(s) 134, 287, 535, 905
BslFI GGGAC 2 cut(s) 80, 950
BslI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 2 cut(s) 80, 950
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 5 cut(s) 64, 70, 163, 889, 1003
BspFNI CGCG 1 cut(s) 993
BspLI GGNNCC 5 cut(s) 153, 591, 735, 812, 903
BspPI GGATC 5 cut(s) 96, 212, 584, 597, 655
BspT104I TTCGAA 1 cut(s) 553
BspT107I GGYRCC 2 cut(s) 151, 901
BspTNI GGTCTC 1 cut(s) 350
BsrI ACTGG 1 cut(s) 298
BssECI CCNNGG 2 cut(s) 594, 642
BssMI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BssNI GRCGYC 3 cut(s) 19, 302, 387
Bst4CI ACNGT 2 cut(s) 214, 449
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 3 cut(s) 19, 302, 387
BstBAI YACGTR 1 cut(s) 876
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 5 cut(s) 51, 57, 171, 897, 1011
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 5 cut(s) 68, 129, 276, 403, 487
BstFNI CGCG 1 cut(s) 993
BstKTI GATC 7 cut(s) 6, 91, 220, 461, 592, 650, 784
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
BstMWI GCNNNNNNNGC 1 cut(s) 810
BstNSI RCATGY 1 cut(s) 830
BstSCI CCNGG 3 cut(s) 132, 285, 534
BstSFI CTRYAG 1 cut(s) 842
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 1 cut(s) 993
BstV1I GCAGC 3 cut(s) 490, 791, 997
BstX2I RGATCY 2 cut(s) 589, 647
BstXI CCANNNNNNTGG 1 cut(s) 807
BstYI RGATCY 2 cut(s) 589, 647
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 642
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 5 cut(s) 68, 129, 276, 403, 487
BtsIMutI CAGTG 1 cut(s) 243
Cac8I GCNNGC 1 cut(s) 664
CaiI CAGNNNCTG 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 733
Csp6I GTAC 1 cut(s) 952
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 4 cut(s) 440, 628, 801, 827
CviQI GTAC 1 cut(s) 952
DdeI CTNAG 5 cut(s) 51, 57, 171, 897, 1011
DpnI GATC 7 cut(s) 5, 90, 219, 460, 591, 649, 783
DpnII GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 350
Eco47I GGWCC 1 cut(s) 733
Eco81I CCTNAGG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 593
EcoO109I RGGNCCY 1 cut(s) 733
EcoT22I ATGCAT 1 cut(s) 566
FaeI CATG 4 cut(s) 443, 631, 804, 830
FaqI GGGAC 2 cut(s) 80, 950
FatI CATG 4 cut(s) 439, 627, 800, 826
FauNDI CATATG 2 cut(s) 226, 766
Fnu4HI GCNGC 3 cut(s) 504, 805, 986
FokI GGATG 5 cut(s) 75, 116, 263, 410, 494
Fsp4HI GCNGC 3 cut(s) 504, 805, 986
FspBI CTAG 3 cut(s) 368, 407, 456
GluI GCNGC 3 cut(s) 504, 805, 986
GsuI CTGGAG 1 cut(s) 829
HaeIII GGCC 1 cut(s) 128
HapII CCGG 4 cut(s) 134, 287, 535, 905
Hin1I GRCGYC 3 cut(s) 19, 302, 387
Hin1II CATG 4 cut(s) 443, 631, 804, 830
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 6 cut(s) 35, 104, 161, 167, 320, 857
HpaII CCGG 4 cut(s) 134, 287, 535, 905
HphI GGTGA 3 cut(s) 83, 423, 879
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 7 cut(s) 34, 166, 172, 463, 856, 862, 1002
Hpy188III TCNNGA 4 cut(s) 593, 681, 689, 703
Hpy8I GTNNAC 1 cut(s) 17
Hpy99I CGWCG 2 cut(s) 307, 392
HpyAV CCTTC 4 cut(s) 39, 579, 744, 921
HpyCH4III ACNGT 2 cut(s) 214, 449
HpyCH4IV ACGT 4 cut(s) 19, 302, 387, 875
HpyCH4V TGCA 7 cut(s) 209, 224, 403, 528, 564, 722, 826
HpyF10VI GCNNNNNNNGC 1 cut(s) 810
HpyF3I CTNAG 5 cut(s) 51, 57, 171, 897, 1011
HpySE526I ACGT 4 cut(s) 19, 302, 387, 875
Hsp92I GRCGYC 3 cut(s) 19, 302, 387
Hsp92II CATG 4 cut(s) 443, 631, 804, 830
Kzo9I GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
LmnI GCTCC 3 cut(s) 802, 810, 982
Lsp1109I GCAGC 3 cut(s) 490, 791, 997
LweI GCATC 2 cut(s) 412, 731
MaeI CTAG 3 cut(s) 368, 407, 456
MaeII ACGT 4 cut(s) 19, 302, 387, 875
MaeIII GTNAC 3 cut(s) 281, 360, 443
MalI GATC 7 cut(s) 5, 90, 219, 460, 591, 649, 783
MboI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
MboII GAAGA 4 cut(s) 33, 184, 434, 560
MflI RGATCY 2 cut(s) 589, 647
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 5 cut(s) 510, 612, 696, 708, 847
MluI ACGCGT 1 cut(s) 991
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 3 cut(s) 66, 375, 459
Mph1103I ATGCAT 1 cut(s) 566
MroXI GAANNNNTTC 1 cut(s) 972
MspI CCGG 4 cut(s) 134, 287, 535, 905
MspR9I CCNGG 3 cut(s) 134, 287, 536
Mva1269I GAATGC 1 cut(s) 564
MvnI CGCG 1 cut(s) 993
MwoI GCNNNNNNNGC 1 cut(s) 810
NciI CCSGG 3 cut(s) 134, 287, 536
NdeI CATATG 2 cut(s) 226, 766
NdeII GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
NlaIII CATG 4 cut(s) 443, 631, 804, 830
NlaIV GGNNCC 5 cut(s) 153, 591, 735, 812, 903
NmuCI GTSAC 1 cut(s) 360
NsiI ATGCAT 1 cut(s) 566
NspI RCATGY 1 cut(s) 830
NspV TTCGAA 1 cut(s) 553
PctI GAATGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 5 cut(s) 35, 104, 161, 167, 857
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 3 cut(s) 505, 806, 987
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Ppu21I YACGTR 1 cut(s) 876
PpuMI RGGWCCY 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 733
PspN4I GGNNCC 5 cut(s) 153, 591, 735, 812, 903
PspPI GGNCC 1 cut(s) 733
PspPPI RGGWCCY 1 cut(s) 733
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 589, 647
RsaI GTAC 1 cut(s) 953
RsaNI GTAC 1 cut(s) 952
SatI GCNGC 3 cut(s) 504, 805, 986
Sau3AI GATC 7 cut(s) 3, 88, 217, 458, 589, 647, 781
Sau96I GGNCC 1 cut(s) 733
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 3 cut(s) 134, 287, 536
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 2 cut(s) 412, 731
SfcI CTRYAG 1 cut(s) 842
SfuI TTCGAA 1 cut(s) 553
SinI GGWCC 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 5 cut(s) 510, 612, 696, 708, 847
SspMI CTAG 3 cut(s) 368, 407, 456
StyD4I CCNGG 3 cut(s) 132, 285, 534
TaaI ACNGT 2 cut(s) 214, 449
TaiI ACGT 4 cut(s) 22, 305, 390, 878
TaqI TCGA 3 cut(s) 231, 390, 553
TasI AATT 5 cut(s) 510, 612, 696, 708, 847
TfiI GAWTC 5 cut(s) 35, 104, 161, 167, 857
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 3 cut(s) 503, 804, 985
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 6 cut(s) 33, 264, 536, 616, 708, 783
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 733
XceI RCATGY 1 cut(s) 830
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 3 cut(s) 368, 407, 456
ZraI GACGTC 3 cut(s) 20, 303, 388
Zsp2I ATGCAT 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.