MD07G1002700.v1.1

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005345
Physical Location & Seq
Forward (+)
289870 .. 293909
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1002700.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGATCTTTGTTGATGGGCTTCCCTTTTCTACAGACTCTTCTGCATCGCCTCAGGGACGGGATACTCTGGTTGCTCTACTGGAACACCCTGCTTTGGTAGAAGCCTCAAATTCGTTCAAAGCTATCCCGGAGAGGAGATTCTCTGTACGTGAAGAGTCAGTTCTGGACACATCGCCACGTAGTAAATGGGTTTATTTGTTCCAGAGAGAATATGCAACTGTTGATCCGGCATTGGTGGATTTTGTTGGAACTGATGAAGCAACAACCTGTATCGGTGTGGCCATCTGGAATCCGAGAAATGGAATGACCTCAGTTGCTCATATGGATTCTCCAAACATTGTGGACACGGGTTTATCCCAAATGTTGTCACTTCTTGTTGACCATAACACGGATATGGAGTTAGATGTGCATCTAGTTGGTGGATTTGAAGATGTTTCAACTAATGTTTGCTCCAGGATCCTCTACTTTGTTATTGATTTTCCAGAGCATGGTAACTACAACACTGGATCAGAAAGTCAGGAAAACTTGGATGGTTATTCCTTCCCTTTAGGTGCCAAAATTGTTGAAACTTTATGGAAGAGACCGGAGAAATTCCATATACGTACTCTATGCATTCTTGGGCATAACACTAGAAGGGATCCCGAGGGAAATGCGTACCCAAACTTCAACGGTTTCATGATAGGAACTTCCACCGGATCTATTACCCCAGCTATCTTTGACAAAACCTCAAGATGCCCTGATGAAGTTGTCAGGAGAATTCGAGTTTCTGTATCATATGAGGACTCTAGTTGGAAAGGAAAGTTACTAGAAACATATGATACTCAAACTGATCATTTCAAGATAGCTCCATGTTGCTGGACACTGCAGCAGTTACATTTTGCTTTGTCATTACAACACTACTCTGATTCAGAAATCCTCCTCATGTGTTCTACTTCACCCTCAGCTGAGAACCCAGATTTTGTGGAGAATATGAGAAGGCAATGGGACTACCTAATTAAACACCCAGATTGGAGAGAAACCTTTCCCATGAAGCAGCCACGCATATTTGAGAGGACTGCTGAAGGTGGCTGGAGAAAGCCACAAGGTGCTGATACATCAATCAGTAGGAGGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

372

Amino Acids

42.11

Weight (kDa)

5.26

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 59 - 358 1.1e-82 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AccB7I CCANNNNNTGG 1 cut(s) 488
AclWI GGATC 7 cut(s) 218, 451, 464, 514, 632, 645, 703
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 109, 590, 756
AcuI CTGAAG 1 cut(s) 1080
AdeI CACNNNGTG 1 cut(s) 1085
AfaI GTAC 3 cut(s) 147, 604, 656
AfiI CCNNNNNNNGG 4 cut(s) 94, 299, 388, 488
AgsI TTSAA 6 cut(s) 118, 428, 438, 566, 667, 838
AjnI CCWGG 1 cut(s) 452
AluBI AGCT 4 cut(s) 122, 710, 845, 944
AluI AGCT 4 cut(s) 122, 710, 845, 944
Alw26I GTCTC 1 cut(s) 574
AlwI GGATC 7 cut(s) 218, 451, 464, 514, 632, 645, 703
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 865, 1033
ApoI RAATTY 3 cut(s) 109, 590, 756
Asp700I GAANNNNTTC 1 cut(s) 1020
AsuC2I CCSGG 1 cut(s) 128
AsuHPI GGTGA 1 cut(s) 927
AvaI CYCGRG 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 51
BaeI ACNNNNGTAYC 2 cut(s) 54, 87
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 2 cut(s) 456, 637
BanI GGYRCC 1 cut(s) 551
BarI GAAGNNNNNNTAC 2 cut(s) 647, 679
BbvCI CCTCAGC 1 cut(s) 940
BbvI GCAGC 2 cut(s) 877, 1045
BccI CCATC 3 cut(s) 8, 290, 524
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BciT130I CCWGG 1 cut(s) 454
BciVI GTATCC 1 cut(s) 55
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 574
BfaI CTAG 5 cut(s) 413, 630, 786, 806, 1114
BfmI CTRYAG 2 cut(s) 30, 863
BfuI GTATCC 1 cut(s) 55
BisI GCNGC 2 cut(s) 866, 1034
BlsI GCNGC 2 cut(s) 867, 1035
Bme1390I CCNGG 2 cut(s) 128, 454
BmeT110I CYCGRG 1 cut(s) 641
BmiI GGNNCC 3 cut(s) 458, 553, 639
BmrFI CCNGG 2 cut(s) 128, 454
BmsI GCATC 3 cut(s) 53, 418, 722
BpmI CTGGAG 2 cut(s) 436, 1090
Bpu10I CCTNAGC 1 cut(s) 940
BpuEI CTTGAG 1 cut(s) 712
BpuMI CCSGG 1 cut(s) 128
BsaAI YACGTR 3 cut(s) 149, 179, 602
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 642
BsaWI WCCGGW 2 cut(s) 583, 692
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 299, 388, 488
Bse1I ACTGG 2 cut(s) 84, 508
Bse21I CCTNAGG 1 cut(s) 51
Bse3DI GCAATG 1 cut(s) 986
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 1 cut(s) 454
BseDI CCNNGG 1 cut(s) 642
BseGI GGATG 1 cut(s) 535
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 4 cut(s) 94, 299, 388, 488
BseMI GCAATG 1 cut(s) 986
BseMII CTCAG 4 cut(s) 65, 324, 936, 954
BseNI ACTGG 2 cut(s) 84, 508
BseRI GAGGAG 2 cut(s) 148, 908
BseXI GCAGC 2 cut(s) 877, 1045
BseYI CCCAGC 1 cut(s) 706
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 551
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 4 cut(s) 128, 227, 584, 693
BslFI GGGAC 2 cut(s) 69, 998
BslI CCNNNNNNNGG 4 cut(s) 94, 299, 388, 488
BsmAI GTCTC 1 cut(s) 574
BsmFI GGGAC 2 cut(s) 69, 998
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 574
BsoBI CYCGRG 1 cut(s) 641
Bsp143I GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 4 cut(s) 64, 323, 937, 953
BspHI TCATGA 1 cut(s) 675
BspLI GGNNCC 3 cut(s) 458, 553, 639
BspMAI CTGCAG 1 cut(s) 867
BspPI GGATC 7 cut(s) 218, 451, 464, 514, 632, 645, 703
BspT107I GGYRCC 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 986
BsrI ACTGG 2 cut(s) 84, 508
BssECI CCNNGG 1 cut(s) 642
BssMI GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
Bst2UI CCWGG 1 cut(s) 454
Bst4CI ACNGT 2 cut(s) 220, 671
Bst6I CTCTTC 3 cut(s) 43, 147, 572
BstBAI YACGTR 3 cut(s) 149, 179, 602
BstDEI CTNAG 4 cut(s) 51, 310, 940, 945
BstF5I GGATG 1 cut(s) 535
BstKTI GATC 7 cut(s) 6, 226, 459, 509, 640, 698, 832
BstMAI GTCTC 1 cut(s) 574
BstMBI GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
BstNI CCWGG 1 cut(s) 454
BstSCI CCNGG 2 cut(s) 126, 452
BstSFI CTRYAG 2 cut(s) 30, 863
BstSNI TACGTA 1 cut(s) 602
BstV1I GCAGC 2 cut(s) 877, 1045
BstX2I RGATCY 3 cut(s) 456, 637, 695
BstXI CCANNNNNNTGG 1 cut(s) 855
BstYI RGATCY 3 cut(s) 456, 637, 695
Bsu36I CCTNAGG 1 cut(s) 51
BsuI GTATCC 1 cut(s) 55
BsuRI GGCC 1 cut(s) 281
BtgZI GCGATG 2 cut(s) 30, 156
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 860
BtsIMutI CAGTG 2 cut(s) 501, 860
CciI TCATGA 1 cut(s) 675
Csp6I GTAC 3 cut(s) 146, 603, 655
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 5 cut(s) 488, 676, 849, 922, 1027
CviQI GTAC 3 cut(s) 146, 603, 655
DdeI CTNAG 4 cut(s) 51, 310, 940, 945
DpnI GATC 7 cut(s) 5, 225, 458, 508, 639, 697, 831
DpnII GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
DraIII CACNNNGTG 1 cut(s) 1085
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 3 cut(s) 43, 147, 572
EarI CTCTTC 3 cut(s) 43, 147, 572
Eco105I TACGTA 1 cut(s) 602
Eco31I GGTCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 1080
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 641
EcoRI GAATTC 1 cut(s) 756
EcoRII CCWGG 1 cut(s) 452
EcoT22I ATGCAT 1 cut(s) 614
FaeI CATG 5 cut(s) 491, 679, 852, 925, 1030
FaqI GGGAC 2 cut(s) 69, 998
FatI CATG 5 cut(s) 487, 675, 848, 921, 1026
FauNDI CATATG 3 cut(s) 321, 775, 814
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 2 cut(s) 866, 1034
FokI GGATG 1 cut(s) 542
Fsp4HI GCNGC 2 cut(s) 866, 1034
FspBI CTAG 5 cut(s) 413, 630, 786, 806, 1114
GluI GCNGC 2 cut(s) 866, 1034
GsaI CCCAGC 1 cut(s) 710
GsuI CTGGAG 2 cut(s) 436, 1090
HaeIII GGCC 1 cut(s) 281
HapII CCGG 4 cut(s) 128, 227, 584, 693
Hin1II CATG 5 cut(s) 491, 679, 852, 925, 1030
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 7 cut(s) 35, 138, 155, 289, 326, 782, 905
HpaII CCGG 4 cut(s) 128, 227, 584, 693
HphI GGTGA 1 cut(s) 927
Hpy166II GTNNAC 2 cut(s) 343, 379
Hpy188I TCNGA 4 cut(s) 294, 511, 904, 910
Hpy8I GTNNAC 2 cut(s) 343, 379
HpyAV CCTTC 4 cut(s) 550, 627, 969, 1055
HpyCH4III ACNGT 2 cut(s) 220, 671
HpyCH4IV ACGT 3 cut(s) 148, 178, 601
HpyCH4V TGCA 5 cut(s) 44, 215, 409, 612, 865
HpyF3I CTNAG 4 cut(s) 51, 310, 940, 945
HpySE526I ACGT 3 cut(s) 148, 178, 601
Hsp92II CATG 5 cut(s) 491, 679, 852, 925, 1030
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
LmnI GCTCC 2 cut(s) 455, 850
Lsp1109I GCAGC 2 cut(s) 877, 1045
LweI GCATC 3 cut(s) 53, 418, 722
MaeI CTAG 5 cut(s) 413, 630, 786, 806, 1114
MaeII ACGT 3 cut(s) 148, 178, 601
MaeIII GTNAC 4 cut(s) 366, 491, 801, 870
MalI GATC 7 cut(s) 5, 225, 458, 508, 639, 697, 831
MboI GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
MboII GAAGA 4 cut(s) 30, 164, 440, 589
MflI RGATCY 3 cut(s) 456, 637, 695
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 5 cut(s) 109, 558, 590, 756, 993
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 3 cut(s) 29, 164, 776
MmeI TCCRAC 2 cut(s) 226, 770
Mox20I TGGCCA 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 614
MroXI GAANNNNTTC 1 cut(s) 1020
MscI TGGCCA 1 cut(s) 281
MseI TTAA 1 cut(s) 996
MslI CAYNNNNRTG 1 cut(s) 392
Msp20I TGGCCA 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 4 cut(s) 128, 227, 584, 693
MspR9I CCNGG 2 cut(s) 128, 454
Mva1269I GAATGC 1 cut(s) 612
MvaI CCWGG 1 cut(s) 454
NciI CCSGG 1 cut(s) 128
NdeI CATATG 3 cut(s) 321, 775, 814
NdeII GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
NlaIII CATG 5 cut(s) 491, 679, 852, 925, 1030
NlaIV GGNNCC 3 cut(s) 458, 553, 639
NmuCI GTSAC 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 614
PagI TCATGA 1 cut(s) 675
PctI GAATGC 1 cut(s) 612
PdmI GAANNNNTTC 1 cut(s) 1020
PfeI GAWTC 4 cut(s) 138, 289, 326, 905
PflMI CCANNNNNTGG 1 cut(s) 488
PfoI TCCNGGA 2 cut(s) 126, 452
PkrI GCNGC 2 cut(s) 867, 1035
PleI GAGTC 3 cut(s) 29, 163, 776
PpsI GAGTC 3 cut(s) 29, 163, 776
Ppu21I YACGTR 3 cut(s) 149, 179, 602
Psp6I CCWGG 1 cut(s) 452
PspFI CCCAGC 1 cut(s) 706
PspGI CCWGG 1 cut(s) 452
PspN4I GGNNCC 3 cut(s) 458, 553, 639
PstI CTGCAG 1 cut(s) 867
PsuI RGATCY 3 cut(s) 456, 637, 695
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 3 cut(s) 147, 604, 656
RsaNI GTAC 3 cut(s) 146, 603, 655
RseI CAYNNNNRTG 1 cut(s) 392
SaqAI TTAA 1 cut(s) 996
SatI GCNGC 2 cut(s) 866, 1034
Sau3AI GATC 7 cut(s) 3, 223, 456, 506, 637, 695, 829
SchI GAGTC 3 cut(s) 29, 164, 776
ScrFI CCNGG 2 cut(s) 128, 454
SfaNI GCATC 3 cut(s) 53, 418, 722
SfcI CTRYAG 2 cut(s) 30, 863
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 1 cut(s) 727
SmoI CTYRAG 1 cut(s) 727
SnaBI TACGTA 1 cut(s) 602
Sse9I AATT 5 cut(s) 109, 558, 590, 756, 993
SspMI CTAG 5 cut(s) 413, 630, 786, 806, 1114
StyD4I CCNGG 2 cut(s) 126, 452
TaaI ACNGT 2 cut(s) 220, 671
TaiI ACGT 3 cut(s) 151, 181, 604
TaqI TCGA 1 cut(s) 760
TasI AATT 5 cut(s) 109, 558, 590, 756, 993
TfiI GAWTC 4 cut(s) 138, 289, 326, 905
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TscAI CASTG 2 cut(s) 508, 867
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 2 cut(s) 865, 1033
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 4 cut(s) 270, 664, 756, 1043
TspGWI ACGGA 1 cut(s) 404
TspRI CASTG 2 cut(s) 508, 867
Van91I CCANNNNNTGG 1 cut(s) 488
XapI RAATTY 3 cut(s) 109, 590, 756
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 1020
XspI CTAG 5 cut(s) 413, 630, 786, 806, 1114
Zsp2I ATGCAT 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.