Rh1DG003300

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005345
Physical Location & Seq
Forward (+)
501850 .. 504613
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG003300.1

Sequence Viewer

Length: 864 bp
ATGATCTTTGTCGGTGGACTTCCTTTCCCTTCCGAATCATCTTCATCTTCTCAGCCTCAGGGACGGGACACCCTCCAAGCTTTGTTGGATCACCCGTTCTTGGAATCTGCCTCCAGTACATTCAAGGCCATCCCGGAGAGGAAGTTCTCGGTGCCCCAAGAATCAGAATCAGAGAGGAGATGGGTTTATCTCTTCCAAAGAGAATATGCAACTGTTGATCCTGCATATGTCGATTTTGTTGGCACTGATGAAGCAACAACCTGTATGGGTCTTGCCATCCGTAACCCGGAAACTGGAATGACGTCCGTTGCCCATTTAGACTCTCCAAAAGTTGTGGATATGGGTCTCTCCCAGATGGTGTCACTACTAGTTCAACCCAATAAGGACGTGGAGTTGGATGTGCATCTAGTTGGTGGTTTTGAAGATGTTTCACCTAATCATGGTAACTGTAATACTAGATCAGAAAATCAGGCAAAGTTGGATGGTTATTCCTTTCCTTTAGCTGCCAAAATTGTTGAAACATTATGCAAGAGCCGGGAGAGATTTCATATTCGAACTCTATGCATTCTTCGGCATAACACCAGAAGGGATCCCGAGGGAAATGCATACCCAATTTTCAACGGGTTCATGGTTAGCACTTCCACTGGATCTATTATTCCTGCCAGCTTTGACGAAACCTCAAGATGTCCTGATGAAATTGTCAGGAGAATTAGAGTTTCTGCATCTTATGAGGACTCCAGATGGAAAGGAAGGGTGTGGGACTACTTGATTGAGTACCCAGATTGGAGAGAAACCTTTCCTATGGAGCAGCCACGCGTATTTCAGAGGACTGCTGAGGGAGGCTGGAAAGCCAAAAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

287

Amino Acids

32.5

Weight (kDa)

5.62

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 53 - 243 7e-54 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 305
AccB1I GGYRCC 1 cut(s) 151
AccII CGCG 1 cut(s) 816
AclWI GGATC 5 cut(s) 96, 212, 584, 597, 655
AcyI GRCGYC 1 cut(s) 302
AfaI GTAC 2 cut(s) 118, 776
AfiI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
AflIII ACRYGT 1 cut(s) 814
AgsI TTSAA 5 cut(s) 124, 374, 422, 518, 619
AhlI ACTAGT 1 cut(s) 367
AjiI CACGTC 1 cut(s) 388
AloI GAACNNNNNNTCC 4 cut(s) 80, 112, 128, 160
AluBI AGCT 3 cut(s) 80, 503, 666
AluI AGCT 3 cut(s) 80, 503, 666
Alw26I GTCTC 1 cut(s) 350
AlwI GGATC 5 cut(s) 96, 212, 584, 597, 655
Ama87I CYCGRG 1 cut(s) 593
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 503, 808
Asp700I GAANNNNTTC 1 cut(s) 795
AsuC2I CCSGG 3 cut(s) 134, 287, 536
AsuHPI GGTGA 2 cut(s) 83, 423
AsuII TTCGAA 1 cut(s) 553
AvaI CYCGRG 1 cut(s) 593
AxyI CCTNAGG 1 cut(s) 57
BaeGI GKGCMC 1 cut(s) 156
BamHI GGATCC 1 cut(s) 589
BanI GGYRCC 1 cut(s) 151
BbvCI CCTCAGC 1 cut(s) 834
BbvI GCAGC 2 cut(s) 490, 820
BccI CCATC 6 cut(s) 137, 174, 284, 349, 476, 735
BcgI CGANNNNNNTGC 4 cut(s) 543, 577, 584, 618
BcnI CCSGG 3 cut(s) 134, 287, 536
BcoDI GTCTC 1 cut(s) 350
BcuI ACTAGT 1 cut(s) 367
BfaI CTAG 3 cut(s) 368, 407, 456
BisI GCNGC 2 cut(s) 504, 809
BlsI GCNGC 2 cut(s) 505, 810
Bme1390I CCNGG 3 cut(s) 134, 287, 536
BmeT110I CYCGRG 1 cut(s) 593
BmgBI CACGTC 1 cut(s) 388
BmiI GGNNCC 2 cut(s) 153, 591
BmrFI CCNGG 3 cut(s) 134, 287, 536
BmsI GCATC 2 cut(s) 412, 731
BpmI CTGGAG 2 cut(s) 97, 721
Bpu10I CCTNAGC 1 cut(s) 834
Bpu14I TTCGAA 1 cut(s) 553
BpuEI CTTGAG 1 cut(s) 664
BpuMI CCSGG 3 cut(s) 134, 287, 536
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 350
BsaJI CCNNGG 1 cut(s) 594
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
Bse1I ACTGG 3 cut(s) 114, 298, 649
Bse21I CCTNAGG 1 cut(s) 57
Bse8I GATNNNNATC 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 4 cut(s) 129, 276, 403, 487
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
BseMII CTCAG 3 cut(s) 65, 71, 825
BseNI ACTGG 3 cut(s) 114, 298, 649
BseRI GAGGAG 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 2 cut(s) 490, 820
Bsh1236I CGCG 1 cut(s) 816
BshFI GGCC 1 cut(s) 128
BshNI GGYRCC 1 cut(s) 151
BsiHKCI CYCGRG 1 cut(s) 593
BsiSI CCGG 3 cut(s) 134, 287, 535
BslFI GGGAC 3 cut(s) 75, 80, 773
BslI CCNNNNNNNGG 4 cut(s) 100, 286, 293, 440
BsmAI GTCTC 1 cut(s) 350
BsmFI GGGAC 3 cut(s) 75, 80, 773
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 1 cut(s) 128
Bso31I GGTCTC 1 cut(s) 350
BsoBI CYCGRG 1 cut(s) 593
Bsp119I TTCGAA 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 6 cut(s) 3, 88, 217, 458, 589, 647
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 3 cut(s) 64, 70, 826
BspFNI CGCG 1 cut(s) 816
BspLI GGNNCC 2 cut(s) 153, 591
BspPI GGATC 5 cut(s) 96, 212, 584, 597, 655
BspT104I TTCGAA 1 cut(s) 553
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 350
BsrI ACTGG 3 cut(s) 114, 298, 649
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 6 cut(s) 3, 88, 217, 458, 589, 647
BssNI GRCGYC 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 214, 449
Bst6I CTCTTC 1 cut(s) 197
BstACI GRCGYC 1 cut(s) 302
BstBI TTCGAA 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 664
BstDEI CTNAG 3 cut(s) 51, 57, 834
BstF5I GGATG 4 cut(s) 129, 276, 403, 487
BstFNI CGCG 1 cut(s) 816
BstKTI GATC 6 cut(s) 6, 91, 220, 461, 592, 650
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 6 cut(s) 3, 88, 217, 458, 589, 647
BstSCI CCNGG 3 cut(s) 132, 285, 534
BstSLI GKGCMC 1 cut(s) 156
BstUI CGCG 1 cut(s) 816
BstV1I GCAGC 2 cut(s) 490, 820
BstX2I RGATCY 2 cut(s) 589, 647
BstYI RGATCY 2 cut(s) 589, 647
Bsu36I CCTNAGG 1 cut(s) 57
BsuRI GGCC 1 cut(s) 128
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 4 cut(s) 129, 276, 403, 487
BtsIMutI CAGTG 2 cut(s) 243, 642
Cac8I GCNNGC 1 cut(s) 664
Csp6I GTAC 2 cut(s) 117, 775
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 2 cut(s) 440, 628
CviJI RGCY 9 cut(s) 55, 80, 128, 503, 534, 666, 811, 843, 851
CviKI_1 RGCY 9 cut(s) 55, 80, 128, 503, 534, 666, 811, 843, 851
CviQI GTAC 2 cut(s) 117, 775
DdeI CTNAG 3 cut(s) 51, 57, 834
DpnI GATC 6 cut(s) 5, 90, 219, 460, 591, 649
DpnII GATC 6 cut(s) 3, 88, 217, 458, 589, 647
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco31I GGTCTC 1 cut(s) 350
Eco81I CCTNAGG 1 cut(s) 57
Eco88I CYCGRG 1 cut(s) 593
EcoT22I ATGCAT 2 cut(s) 566, 607
FaeI CATG 2 cut(s) 443, 631
FaqI GGGAC 3 cut(s) 75, 80, 773
FatI CATG 2 cut(s) 439, 627
FauNDI CATATG 1 cut(s) 226
Fnu4HI GCNGC 2 cut(s) 504, 809
FokI GGATG 4 cut(s) 116, 263, 410, 494
Fsp4HI GCNGC 2 cut(s) 504, 809
FspBI CTAG 3 cut(s) 368, 407, 456
GluI GCNGC 2 cut(s) 504, 809
GsuI CTGGAG 2 cut(s) 97, 721
HaeIII GGCC 1 cut(s) 128
HapII CCGG 3 cut(s) 134, 287, 535
Hin1I GRCGYC 1 cut(s) 302
Hin1II CATG 2 cut(s) 443, 631
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 6 cut(s) 35, 104, 161, 167, 320, 734
HpaII CCGG 3 cut(s) 134, 287, 535
HphI GGTGA 2 cut(s) 83, 423
Hpy166II GTNNAC 1 cut(s) 17
Hpy188I TCNGA 5 cut(s) 34, 166, 172, 463, 825
Hpy188III TCNNGA 5 cut(s) 593, 681, 689, 703, 738
Hpy8I GTNNAC 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 39, 579, 744
HpyCH4III ACNGT 2 cut(s) 214, 449
HpyCH4IV ACGT 2 cut(s) 302, 387
HpyCH4V TGCA 7 cut(s) 209, 224, 403, 528, 564, 605, 722
HpyF3I CTNAG 3 cut(s) 51, 57, 834
HpySE526I ACGT 2 cut(s) 302, 387
Hsp92I GRCGYC 1 cut(s) 302
Hsp92II CATG 2 cut(s) 443, 631
Kzo9I GATC 6 cut(s) 3, 88, 217, 458, 589, 647
LmnI GCTCC 1 cut(s) 805
Lsp1109I GCAGC 2 cut(s) 490, 820
LweI GCATC 2 cut(s) 412, 731
MaeI CTAG 3 cut(s) 368, 407, 456
MaeII ACGT 2 cut(s) 302, 387
MaeIII GTNAC 3 cut(s) 281, 360, 443
MalI GATC 6 cut(s) 5, 90, 219, 460, 591, 649
MboI GATC 6 cut(s) 3, 88, 217, 458, 589, 647
MboII GAAGA 5 cut(s) 33, 39, 184, 434, 560
MflI RGATCY 2 cut(s) 589, 647
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 4 cut(s) 510, 612, 696, 708
MluI ACGCGT 1 cut(s) 814
MlyI GAGTC 2 cut(s) 314, 728
MmeI TCCRAC 3 cut(s) 66, 375, 459
Mph1103I ATGCAT 2 cut(s) 566, 607
MroXI GAANNNNTTC 1 cut(s) 795
MspI CCGG 3 cut(s) 134, 287, 535
MspR9I CCNGG 3 cut(s) 134, 287, 536
Mva1269I GAATGC 1 cut(s) 564
MvnI CGCG 1 cut(s) 816
NciI CCSGG 3 cut(s) 134, 287, 536
NdeI CATATG 1 cut(s) 226
NdeII GATC 6 cut(s) 3, 88, 217, 458, 589, 647
NlaIII CATG 2 cut(s) 443, 631
NlaIV GGNNCC 2 cut(s) 153, 591
NmuCI GTSAC 1 cut(s) 360
NsiI ATGCAT 2 cut(s) 566, 607
NspV TTCGAA 1 cut(s) 553
PctI GAATGC 1 cut(s) 564
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 4 cut(s) 35, 104, 161, 167
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 2 cut(s) 505, 810
PleI GAGTC 2 cut(s) 314, 728
PpsI GAGTC 2 cut(s) 314, 728
PspN4I GGNNCC 2 cut(s) 153, 591
PsuI RGATCY 2 cut(s) 589, 647
RsaI GTAC 2 cut(s) 118, 776
RsaNI GTAC 2 cut(s) 117, 775
SatI GCNGC 2 cut(s) 504, 809
Sau3AI GATC 6 cut(s) 3, 88, 217, 458, 589, 647
SchI GAGTC 2 cut(s) 314, 728
ScrFI CCNGG 3 cut(s) 134, 287, 536
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 2 cut(s) 412, 731
SfuI TTCGAA 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 679
SmoI CTYRAG 1 cut(s) 679
SpeI ACTAGT 1 cut(s) 367
Sse9I AATT 4 cut(s) 510, 612, 696, 708
SspMI CTAG 3 cut(s) 368, 407, 456
StyD4I CCNGG 3 cut(s) 132, 285, 534
TaaI ACNGT 2 cut(s) 214, 449
TaiI ACGT 2 cut(s) 305, 390
TaqI TCGA 2 cut(s) 231, 553
TasI AATT 4 cut(s) 510, 612, 696, 708
TatI WGTACW 1 cut(s) 116
TfiI GAWTC 4 cut(s) 35, 104, 161, 167
TscAI CASTG 2 cut(s) 250, 649
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 2 cut(s) 503, 808
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 5 cut(s) 33, 264, 536, 616, 708
TspGWI ACGGA 2 cut(s) 269, 295
TspRI CASTG 2 cut(s) 250, 649
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 3 cut(s) 368, 407, 456
ZraI GACGTC 1 cut(s) 303
Zsp2I ATGCAT 2 cut(s) 566, 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.