FvH4_7g00170

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005345
Physical Location & Seq
Forward (+)
771653 .. 775304
3652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00170.t2

Sequence Viewer

Length: 1074 bp
ATGATCTTTGTGGACGGACTCCCTTTTGCTTCCGATTCATCTTCGGAGCCTCAGCAGCAGGGATGGGACACCCTCCAAGCTTTGTTGGATCACCCGTCCTTGGAATCTGCCTCCAATGCACTCAAGGCCATCCCAGAAAAAAAGTTCTCGGTGTCTCAAGATTCTGTTCCAGAGAGTAGATGGGTTTATCTCTTCCAAAGAGAATATGCTACTGTCGATCCTGCATTTGTCAATTTTGTTGGCACTGATGAAGCAACAACCTGTATGGGTCTTGCCATCCGTAACCCAGAAAATGGAATGACCTCGGTTGCCCATCTGGACTCTCCAAAAGTTGTGGATATGGGTCTCTCCCAGATGATGTCACTGCTTGTTCAAGACAATATGGACGTGGAGTTAGATGTGCATCTAGTGGGTGGTTTCGAAGATGTTTCACCTAATGTTTGGCCCAGGATCCTCTACTTTCATGGTAACTGTAATACTAGATCAGAAAATCAGGCAAAGTTGGATGGTTATTCCTTTCCTTTAGGTGCCAAAATTATTGAAACATTATTGAAGAGACCGGAGAGATTTCACATTCGAACTCTATGCATTCTTCGGCATAACACTAGGAGGGATCCTGAGGGAAATGCATACCCAATTTTCCACGGGTTCATGATAAGCACTTCCACTGGATCTATTATTCCTGCCAGCTTTGACGAAACCTCAAGATGTCCTGATGAAATTGTCAGGAGAATTCGAGTTTCTGCATCTTATGAGGACTCCAGATGGAAAGGAAGGTTATTGGAAACATATGAAACTCAAACTGATCTTTTCAAAATAGCTCCATGCTGCTGGACCTTTCGTCAGTTGCATATTGCATCATCTTTAAAAAAATATTCAGATTCGGAAATCCTTCTTATGTGTTCTACTTCACCCTCTGCTGAGGCACCAGATTTTGTGAAAAATCTGAGAAGGGGGTGGGACTACTTGCTTGAGTACCCAGATTGGAGAGAAACTTTTCCTATGAAGCAGCCACGCGTATTTGAGAGGACTGCTGAGGGAGGCTGGAAAAGCCAAAAGATGGAGATACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

358

Amino Acids

40.71

Weight (kDa)

5.46

Isoelectric Point (pI)

55.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 54 - 360 3.9e-79 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 527, 925
AccB7I CCANNNNNTGG 2 cut(s) 293, 1060
AccII CGCG 1 cut(s) 1017
AclWI GGATC 7 cut(s) 96, 212, 445, 458, 608, 621, 679
AcsI RAATTY 1 cut(s) 732
AfaI GTAC 1 cut(s) 977
AfiI CCNNNNNNNGG 3 cut(s) 100, 293, 1060
AflIII ACRYGT 1 cut(s) 1015
AgsI TTSAA 4 cut(s) 374, 542, 553, 814
AhdI GACNNNNNGTC 1 cut(s) 840
AjiI CACGTC 1 cut(s) 388
AjnI CCWGG 1 cut(s) 446
AluBI AGCT 3 cut(s) 80, 690, 821
AluI AGCT 3 cut(s) 80, 690, 821
Alw26I GTCTC 3 cut(s) 159, 350, 550
AlwI GGATC 7 cut(s) 96, 212, 445, 458, 608, 621, 679
AoxI GGCC 2 cut(s) 126, 443
ApeKI GCWGC 3 cut(s) 55, 828, 1009
ApoI RAATTY 1 cut(s) 732
Asp700I GAANNNNTTC 2 cut(s) 891, 996
AspS9I GGNCC 2 cut(s) 444, 834
AsuHPI GGTGA 3 cut(s) 83, 423, 903
AsuII TTCGAA 2 cut(s) 420, 577
AvaII GGWCC 1 cut(s) 834
AxyI CCTNAGG 1 cut(s) 618
BamHI GGATCC 2 cut(s) 450, 613
BanI GGYRCC 2 cut(s) 527, 925
BbvCI CCTCAGC 3 cut(s) 51, 921, 1035
BbvI GCAGC 3 cut(s) 67, 815, 1021
BccI CCATC 8 cut(s) 57, 137, 174, 284, 321, 500, 759, 1054
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BciT130I CCWGG 1 cut(s) 448
BcoDI GTCTC 3 cut(s) 159, 350, 550
BfaI CTAG 3 cut(s) 407, 480, 606
BisI GCNGC 3 cut(s) 56, 829, 1010
BlsI GCNGC 3 cut(s) 57, 830, 1011
Bme1390I CCNGG 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 834
BmeRI GACNNNNNGTC 1 cut(s) 840
BmgBI CACGTC 1 cut(s) 388
BmgT120I GGNCC 2 cut(s) 444, 834
BmiI GGNNCC 5 cut(s) 48, 452, 529, 615, 927
BmrFI CCNGG 1 cut(s) 448
BmsI GCATC 3 cut(s) 412, 755, 866
BpmI CTGGAG 1 cut(s) 745
Bpu10I CCTNAGC 3 cut(s) 51, 921, 1035
Bpu14I TTCGAA 2 cut(s) 420, 577
BpuEI CTTGAG 4 cut(s) 107, 141, 688, 992
BsaBI GATNNNNATC 1 cut(s) 402
BsaI GGTCTC 2 cut(s) 350, 550
BsaJI CCNNGG 4 cut(s) 99, 303, 446, 643
BsaWI WCCGGW 1 cut(s) 559
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 293, 1060
Bse1I ACTGG 1 cut(s) 673
Bse21I CCTNAGG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 1 cut(s) 448
BseDI CCNNGG 4 cut(s) 99, 303, 446, 643
BseGI GGATG 4 cut(s) 68, 129, 276, 511
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 3 cut(s) 100, 293, 1060
BseMII CTCAG 5 cut(s) 65, 609, 912, 938, 1026
BseNI ACTGG 1 cut(s) 673
BseXI GCAGC 3 cut(s) 67, 815, 1021
Bsh1236I CGCG 1 cut(s) 1017
BshFI GGCC 2 cut(s) 128, 445
BshNI GGYRCC 2 cut(s) 527, 925
BsiSI CCGG 1 cut(s) 560
BslFI GGGAC 2 cut(s) 80, 974
BslI CCNNNNNNNGG 3 cut(s) 100, 293, 1060
BsmAI GTCTC 3 cut(s) 159, 350, 550
BsmFI GGGAC 2 cut(s) 80, 974
BsmI GAATGC 1 cut(s) 588
BsnI GGCC 2 cut(s) 128, 445
Bso31I GGTCTC 2 cut(s) 350, 550
Bsp119I TTCGAA 2 cut(s) 420, 577
Bsp143I GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
BspANI GGCC 2 cut(s) 128, 445
BspCNI CTCAG 5 cut(s) 64, 610, 913, 939, 1027
BspFNI CGCG 1 cut(s) 1017
BspHI TCATGA 1 cut(s) 651
BspLI GGNNCC 5 cut(s) 48, 452, 529, 615, 927
BspPI GGATC 7 cut(s) 96, 212, 445, 458, 608, 621, 679
BspT104I TTCGAA 2 cut(s) 420, 577
BspT107I GGYRCC 2 cut(s) 527, 925
BspTNI GGTCTC 2 cut(s) 350, 550
BsrI ACTGG 1 cut(s) 673
BssECI CCNNGG 4 cut(s) 99, 303, 446, 643
BssMI GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 2 cut(s) 214, 473
Bst6I CTCTTC 2 cut(s) 197, 548
BstBI TTCGAA 2 cut(s) 420, 577
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 5 cut(s) 51, 618, 921, 947, 1035
BstDSI CCRYGG 1 cut(s) 643
BstF5I GGATG 4 cut(s) 68, 129, 276, 511
BstFNI CGCG 1 cut(s) 1017
BstKTI GATC 8 cut(s) 6, 91, 220, 453, 485, 616, 674, 808
BstMAI GTCTC 3 cut(s) 159, 350, 550
BstMBI GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
BstMWI GCNNNNNNNGC 4 cut(s) 55, 116, 125, 1050
BstNI CCWGG 1 cut(s) 448
BstSCI CCNGG 1 cut(s) 446
BstUI CGCG 1 cut(s) 1017
BstV1I GCAGC 3 cut(s) 67, 815, 1021
BstX2I RGATCY 3 cut(s) 450, 613, 671
BstXI CCANNNNNNTGG 1 cut(s) 831
BstYI RGATCY 3 cut(s) 450, 613, 671
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 2 cut(s) 128, 445
BtgI CCRYGG 1 cut(s) 643
BtrI CACGTC 1 cut(s) 388
BtsCI GGATG 4 cut(s) 68, 129, 276, 511
BtsI GCAGTG 1 cut(s) 362
BtsIMutI CAGTG 3 cut(s) 243, 362, 666
Cac8I GCNNGC 1 cut(s) 688
CciI TCATGA 1 cut(s) 651
Cfr13I GGNCC 2 cut(s) 444, 834
Csp6I GTAC 1 cut(s) 976
CspCI CAANNNNNGTGG 2 cut(s) 315, 350
CviAII CATG 3 cut(s) 464, 652, 825
CviJI RGCY 9 cut(s) 49, 80, 128, 445, 690, 821, 1012, 1044, 1053
CviKI_1 RGCY 9 cut(s) 49, 80, 128, 445, 690, 821, 1012, 1044, 1053
CviQI GTAC 1 cut(s) 976
DdeI CTNAG 5 cut(s) 51, 618, 921, 947, 1035
DpnI GATC 8 cut(s) 5, 90, 219, 452, 484, 615, 673, 807
DpnII GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
DraI TTTAAA 1 cut(s) 867
DriI GACNNNNNGTC 1 cut(s) 840
Eam1104I CTCTTC 2 cut(s) 197, 548
Eam1105I GACNNNNNGTC 1 cut(s) 840
EarI CTCTTC 2 cut(s) 197, 548
Eco130I CCWWGG 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 350, 550
Eco47I GGWCC 1 cut(s) 834
Eco81I CCTNAGG 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 732
EcoRII CCWGG 1 cut(s) 446
EcoT14I CCWWGG 1 cut(s) 99
EcoT22I ATGCAT 2 cut(s) 590, 631
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 3 cut(s) 467, 655, 828
FaqI GGGAC 2 cut(s) 80, 974
FatI CATG 3 cut(s) 463, 651, 824
FauNDI CATATG 1 cut(s) 790
Fnu4HI GCNGC 3 cut(s) 56, 829, 1010
FokI GGATG 4 cut(s) 75, 116, 263, 518
Fsp4HI GCNGC 3 cut(s) 56, 829, 1010
FspBI CTAG 3 cut(s) 407, 480, 606
GluI GCNGC 3 cut(s) 56, 829, 1010
GsuI CTGGAG 1 cut(s) 745
HaeIII GGCC 2 cut(s) 128, 445
HapII CCGG 1 cut(s) 560
Hin1II CATG 3 cut(s) 467, 655, 828
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 7 cut(s) 18, 35, 104, 161, 320, 758, 881
HpaII CCGG 1 cut(s) 560
HphI GGTGA 3 cut(s) 83, 423, 903
Hpy166II GTNNAC 1 cut(s) 13
Hpy188I TCNGA 6 cut(s) 34, 46, 487, 880, 886, 948
Hpy8I GTNNAC 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 768, 902, 945
HpyCH4III ACNGT 2 cut(s) 214, 473
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 8 cut(s) 119, 224, 403, 588, 629, 746, 850, 857
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 116, 125, 1050
HpyF3I CTNAG 5 cut(s) 51, 618, 921, 947, 1035
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 3 cut(s) 467, 655, 828
Kzo9I GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
LmnI GCTCC 2 cut(s) 46, 826
Lsp1109I GCAGC 3 cut(s) 67, 815, 1021
LweI GCATC 3 cut(s) 412, 755, 866
MaeI CTAG 3 cut(s) 407, 480, 606
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 3 cut(s) 281, 360, 467
MalI GATC 8 cut(s) 5, 90, 219, 452, 484, 615, 673, 807
MboI GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
MboII GAAGA 5 cut(s) 33, 184, 434, 565, 584
MflI RGATCY 3 cut(s) 450, 613, 671
MluCI AATT 5 cut(s) 232, 534, 636, 720, 732
MluI ACGCGT 1 cut(s) 1015
MlyI GAGTC 3 cut(s) 12, 314, 752
MmeI TCCRAC 2 cut(s) 66, 483
Mph1103I ATGCAT 2 cut(s) 590, 631
MroXI GAANNNNTTC 2 cut(s) 891, 996
MseI TTAA 1 cut(s) 866
MspI CCGG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 448
Mva1269I GAATGC 1 cut(s) 588
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 1 cut(s) 1017
MwoI GCNNNNNNNGC 4 cut(s) 55, 116, 125, 1050
NdeI CATATG 1 cut(s) 790
NdeII GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
NlaIII CATG 3 cut(s) 467, 655, 828
NlaIV GGNNCC 5 cut(s) 48, 452, 529, 615, 927
NmuCI GTSAC 1 cut(s) 360
NsiI ATGCAT 2 cut(s) 590, 631
NspV TTCGAA 2 cut(s) 420, 577
PagI TCATGA 1 cut(s) 651
PctI GAATGC 1 cut(s) 588
PdmI GAANNNNTTC 2 cut(s) 891, 996
PfeI GAWTC 4 cut(s) 35, 104, 161, 881
PflMI CCANNNNNTGG 2 cut(s) 293, 1060
PkrI GCNGC 3 cut(s) 57, 830, 1011
PleI GAGTC 3 cut(s) 12, 314, 752
PpsI GAGTC 3 cut(s) 12, 314, 752
Psp6I CCWGG 1 cut(s) 446
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 5 cut(s) 48, 452, 529, 615, 927
PspPI GGNCC 2 cut(s) 444, 834
PsuI RGATCY 3 cut(s) 450, 613, 671
RsaI GTAC 1 cut(s) 977
RsaNI GTAC 1 cut(s) 976
SaqAI TTAA 1 cut(s) 866
SatI GCNGC 3 cut(s) 56, 829, 1010
Sau3AI GATC 8 cut(s) 3, 88, 217, 450, 482, 613, 671, 805
Sau96I GGNCC 2 cut(s) 444, 834
SchI GAGTC 3 cut(s) 12, 314, 752
ScrFI CCNGG 1 cut(s) 448
SfaNI GCATC 3 cut(s) 412, 755, 866
SfuI TTCGAA 2 cut(s) 420, 577
SinI GGWCC 1 cut(s) 834
SmlI CTYRAG 4 cut(s) 122, 156, 703, 971
SmoI CTYRAG 4 cut(s) 122, 156, 703, 971
Sse9I AATT 5 cut(s) 232, 534, 636, 720, 732
SspI AATATT 1 cut(s) 875
SspMI CTAG 3 cut(s) 407, 480, 606
StyD4I CCNGG 1 cut(s) 446
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 2 cut(s) 214, 473
TaiI ACGT 1 cut(s) 390
TaqI TCGA 4 cut(s) 216, 420, 577, 736
TasI AATT 5 cut(s) 232, 534, 636, 720, 732
TfiI GAWTC 4 cut(s) 35, 104, 161, 881
Tru1I TTAA 1 cut(s) 866
Tru9I TTAA 1 cut(s) 866
TscAI CASTG 3 cut(s) 250, 369, 673
TseFI GTSAC 1 cut(s) 360
TseI GCWGC 3 cut(s) 55, 828, 1009
Tsp45I GTSAC 1 cut(s) 360
TspDTI ATGAA 7 cut(s) 27, 264, 452, 640, 732, 807, 1019
TspGWI ACGGA 2 cut(s) 30, 269
TspRI CASTG 3 cut(s) 250, 369, 673
Van91I CCANNNNNTGG 2 cut(s) 293, 1060
VpaK11BI GGWCC 1 cut(s) 834
XapI RAATTY 1 cut(s) 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XmnI GAANNNNTTC 2 cut(s) 891, 996
XspI CTAG 3 cut(s) 407, 480, 606
Zsp2I ATGCAT 2 cut(s) 590, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.