Prupe.2G004200_v2.0.a1

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0005345
Physical Location & Seq
Forward (+)
427402 .. 430843
3442 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G004200.3

Sequence Viewer

Length: 1056 bp
ATGATAATTGTTGATGGCCTTCCCTTTTCCACAGACACTTCTGCGTCGCCTCAGGGACGGGATATCCTGGCTGCTTTAATGGAACACCCTGCATTGGTATCTGCCTCAAATTCGTTCAAGGCTATCCCAGAGAGGAGATTCTCAGTACCTGAAGAGTCAGGTCCAGAGAAAACACCACCTAGTAAATGGGTTTATCTATTCCAGAGAGAATATGCAATTGTTGATCCTGCATTGGTGGATTTTGTTGGCACTGATGAAGCAACAACCTGTGTCGGTGTTGCCATTCGGAATCCAGGAAATGGAATGACCTCAGTTGCTCATATGGATTCTCCAAAAATTGTGGACATTGGTCTCTCCCAAATGTTGTCACTCCTTGTTGACCACAATAGTGATAAGGAGTTAGATGTGCATCTGGTTGGTGGCTTTGAAGATGTTTCACCTAATCATGGTAACTGCAACACAAGATCAGAAAGTCAGGAAAAGTTGGCTGGTTATTCCTTTCCTTTATGTGCCAAAATTGTTGAAACTCTATGGAATAGACAGGAAAAGTTTCACATCCGAACTCTCTTCATTCTTGGGCATAACACTAGAAGGGATCTCGAGGGAAATGCATACCCTATTTTCAATGGGTTCATGGTAGGAACTTCGACCGGATCTATTACTCCAGCCAGCTTTGACAGAACCTTAAGATGTCCTGATGAAATTGTCAGGAGAATTCGAGTTTCTGCATCCTACGAGGACTCCAGTTGGAAAGGCAAGTTAATGGAAACATATGATACTCAAACTGACCAATTCAAAATAGCTCCATGCTGCTGGACCCTACGTCAACTAGATATTTCTTTGTCACTACAAGATTATTCTGATCCAGAAATCCTCCTTATGTGTTCTACTTCTCCTTCTGCTGAGGCCCCAGATTTTGTGGAAAATATGAGAAGGCAGTGGGAATACCTGGTTGAACACCCAGATTGGAGAGAAACCTTTCCTATGAAGCAGCCACGTATATTTGAGAGAACTGCTGAAGGAGGCTGGAGAAGGCAAAAGGCGCTGATACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

352

Amino Acids

39.55

Weight (kDa)

5.21

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 299
AclWI GGATC 4 cut(s) 218, 603, 661, 857
AcsI RAATTY 2 cut(s) 109, 714
AcuI CTGAAG 2 cut(s) 171, 1038
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 3 cut(s) 94, 299, 446
AflII CTTAAG 1 cut(s) 685
AgsI TTSAA 6 cut(s) 118, 428, 524, 625, 796, 956
AhdI GACNNNNNGTC 1 cut(s) 822
AjnI CCWGG 3 cut(s) 66, 292, 948
AleI CACNNNNGTG 1 cut(s) 387
AluBI AGCT 2 cut(s) 672, 803
AluI AGCT 2 cut(s) 672, 803
Alw26I GTCTC 1 cut(s) 356
AlwI GGATC 4 cut(s) 218, 603, 661, 857
AlwNI CAGNNNCTG 1 cut(s) 149
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 2 cut(s) 16, 906
ApeKI GCWGC 3 cut(s) 71, 810, 991
ApoI RAATTY 2 cut(s) 109, 714
Asp700I GAANNNNTTC 2 cut(s) 549, 978
AspLEI GCGC 1 cut(s) 1045
AspS9I GGNCC 3 cut(s) 161, 816, 907
AsuHPI GGTGA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 2 cut(s) 161, 816
AxyI CCTNAGG 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 903
BbvI GCAGC 3 cut(s) 58, 797, 1003
BccI CCATC 1 cut(s) 8
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 68, 294, 950
BcoDI GTCTC 1 cut(s) 356
BfaI CTAG 3 cut(s) 180, 588, 830
BfoI RGCGCY 1 cut(s) 1046
BfrI CTTAAG 1 cut(s) 685
BisI GCNGC 3 cut(s) 72, 811, 992
BlsI GCNGC 3 cut(s) 73, 812, 993
Bme1390I CCNGG 3 cut(s) 68, 294, 950
Bme18I GGWCC 2 cut(s) 161, 816
BmeRI GACNNNNNGTC 1 cut(s) 822
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 3 cut(s) 161, 816, 907
BmiI GGNNCC 2 cut(s) 818, 909
BmrFI CCNGG 3 cut(s) 68, 294, 950
BmsI GCATC 2 cut(s) 418, 737
BoxI GACNNNNGTC 1 cut(s) 348
BpmI CTGGAG 3 cut(s) 648, 727, 1048
Bpu10I CCTNAGC 1 cut(s) 903
BsaAI YACGTR 1 cut(s) 998
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 356
BsaWI WCCGGW 1 cut(s) 650
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 299, 446
Bse1I ACTGG 1 cut(s) 744
Bse21I CCTNAGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 3 cut(s) 68, 294, 950
BseGI GGATG 2 cut(s) 555, 728
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 94, 299, 446
BseMII CTCAG 4 cut(s) 65, 156, 324, 894
BseNI ACTGG 1 cut(s) 744
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 3 cut(s) 58, 797, 1003
Bsh1285I CGRYCG 1 cut(s) 651
BshFI GGCC 2 cut(s) 18, 908
BsiEI CGRYCG 1 cut(s) 651
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 1 cut(s) 651
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 94, 299, 446
BsmAI GTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 2 cut(s) 18, 908
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 599
Bsp143I GATC 5 cut(s) 223, 464, 595, 653, 862
BspANI GGCC 2 cut(s) 18, 908
BspCNI CTCAG 4 cut(s) 64, 155, 323, 895
BspLI GGNNCC 2 cut(s) 818, 909
BspPI GGATC 4 cut(s) 218, 603, 661, 857
BspTI CTTAAG 1 cut(s) 685
BspTNI GGTCTC 1 cut(s) 356
BsrI ACTGG 1 cut(s) 744
BssMI GATC 5 cut(s) 223, 464, 595, 653, 862
Bst2UI CCWGG 3 cut(s) 68, 294, 950
Bst6I CTCTTC 2 cut(s) 147, 572
BstAFI CTTAAG 1 cut(s) 685
BstBAI YACGTR 1 cut(s) 998
BstC8I GCNNGC 1 cut(s) 670
BstDEI CTNAG 4 cut(s) 51, 142, 310, 903
BstF5I GGATG 2 cut(s) 555, 728
BstH2I RGCGCY 1 cut(s) 1046
BstHHI GCGC 1 cut(s) 1045
BstKTI GATC 5 cut(s) 226, 467, 598, 656, 865
BstMAI GTCTC 1 cut(s) 356
BstMBI GATC 5 cut(s) 223, 464, 595, 653, 862
BstMCI CGRYCG 1 cut(s) 651
BstMWI GCNNNNNNNGC 1 cut(s) 1042
BstNI CCWGG 3 cut(s) 68, 294, 950
BstPAI GACNNNNGTC 1 cut(s) 348
BstSCI CCNGG 3 cut(s) 66, 292, 948
BstV1I GCAGC 3 cut(s) 58, 797, 1003
BstX2I RGATCY 2 cut(s) 595, 653
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 2 cut(s) 595, 653
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 2 cut(s) 18, 908
BtsCI GGATG 2 cut(s) 555, 728
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 2 cut(s) 249, 944
Cac8I GCNNGC 1 cut(s) 670
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 1 cut(s) 1045
Cfr13I GGNCC 3 cut(s) 161, 816, 907
CseI GACGC 1 cut(s) 33
CsiI ACCWGGT 1 cut(s) 948
Csp6I GTAC 1 cut(s) 146
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 3 cut(s) 446, 634, 807
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 4 cut(s) 51, 142, 310, 903
DpnI GATC 5 cut(s) 225, 466, 597, 655, 864
DpnII GATC 5 cut(s) 223, 464, 595, 653, 862
DriI GACNNNNNGTC 1 cut(s) 822
Eam1104I CTCTTC 2 cut(s) 147, 572
Eam1105I GACNNNNNGTC 1 cut(s) 822
EarI CTCTTC 2 cut(s) 147, 572
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 161, 816
Eco57I CTGAAG 2 cut(s) 171, 1038
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 907
EcoRI GAATTC 1 cut(s) 714
EcoRII CCWGG 3 cut(s) 66, 292, 948
EcoRV GATATC 1 cut(s) 64
EcoT22I ATGCAT 1 cut(s) 613
FaeI CATG 3 cut(s) 449, 637, 810
FaqI GGGAC 1 cut(s) 69
FatI CATG 3 cut(s) 445, 633, 806
FauNDI CATATG 2 cut(s) 321, 772
Fnu4HI GCNGC 3 cut(s) 72, 811, 992
FokI GGATG 2 cut(s) 542, 715
Fsp4HI GCNGC 3 cut(s) 72, 811, 992
FspBI CTAG 3 cut(s) 180, 588, 830
GlaI GCGC 1 cut(s) 1044
GluI GCNGC 3 cut(s) 72, 811, 992
GsuI CTGGAG 3 cut(s) 648, 727, 1048
HaeII RGCGCY 1 cut(s) 1046
HaeIII GGCC 2 cut(s) 18, 908
HapII CCGG 1 cut(s) 651
HgaI GACGC 1 cut(s) 33
HhaI GCGC 1 cut(s) 1045
Hin1II CATG 3 cut(s) 449, 637, 810
Hin6I GCGC 1 cut(s) 1043
HinP1I GCGC 1 cut(s) 1043
HincII GTYRAC 2 cut(s) 379, 827
HindII GTYRAC 2 cut(s) 379, 827
HinfI GANTC 5 cut(s) 138, 155, 289, 326, 740
HpaII CCGG 1 cut(s) 651
HphI GGTGA 1 cut(s) 429
Hpy166II GTNNAC 3 cut(s) 343, 379, 827
Hpy188I TCNGA 4 cut(s) 288, 469, 560, 862
Hpy188III TCNNGA 7 cut(s) 164, 202, 476, 599, 695, 709, 866
Hpy8I GTNNAC 3 cut(s) 343, 379, 827
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 6 cut(s) 29, 585, 906, 927, 1013, 1026
HpyCH4IV ACGT 2 cut(s) 823, 997
HpyCH4V TGCA 7 cut(s) 92, 215, 230, 409, 456, 611, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 1042
HpyF3I CTNAG 4 cut(s) 51, 142, 310, 903
HpySE526I ACGT 2 cut(s) 823, 997
Hsp92II CATG 3 cut(s) 449, 637, 810
HspAI GCGC 1 cut(s) 1043
Kzo9I GATC 5 cut(s) 223, 464, 595, 653, 862
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 3 cut(s) 58, 797, 1003
LweI GCATC 2 cut(s) 418, 737
MabI ACCWGGT 1 cut(s) 948
MaeI CTAG 3 cut(s) 180, 588, 830
MaeII ACGT 2 cut(s) 823, 997
MaeIII GTNAC 3 cut(s) 366, 449, 843
MalI GATC 5 cut(s) 225, 466, 597, 655, 864
MboI GATC 5 cut(s) 223, 464, 595, 653, 862
MboII GAAGA 3 cut(s) 164, 440, 559
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 2 cut(s) 595, 653
MluCI AATT 8 cut(s) 6, 109, 216, 336, 516, 702, 714, 791
MlyI GAGTC 2 cut(s) 164, 734
MmeI TCCRAC 1 cut(s) 728
MnlI CCTC 9 cut(s) 60, 115, 126, 319, 595, 730, 884, 898, 1016
Mph1103I ATGCAT 1 cut(s) 613
MroXI GAANNNNTTC 2 cut(s) 549, 978
MseI TTAA 3 cut(s) 77, 686, 761
MslI CAYNNNNRTG 1 cut(s) 387
MspCI CTTAAG 1 cut(s) 685
MspI CCGG 1 cut(s) 651
MspR9I CCNGG 3 cut(s) 68, 294, 950
MunI CAATTG 1 cut(s) 216
MvaI CCWGG 3 cut(s) 68, 294, 950
MwoI GCNNNNNNNGC 1 cut(s) 1042
NdeI CATATG 2 cut(s) 321, 772
NdeII GATC 5 cut(s) 223, 464, 595, 653, 862
NlaIII CATG 3 cut(s) 449, 637, 810
NlaIV GGNNCC 2 cut(s) 818, 909
NmuCI GTSAC 2 cut(s) 366, 843
NsiI ATGCAT 1 cut(s) 613
OliI CACNNNNGTG 1 cut(s) 387
PaeR7I CTCGAG 1 cut(s) 599
PdmI GAANNNNTTC 2 cut(s) 549, 978
PfeI GAWTC 3 cut(s) 138, 289, 326
PflMI CCANNNNNTGG 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 3 cut(s) 73, 812, 993
PleI GAGTC 2 cut(s) 163, 734
PpsI GAGTC 2 cut(s) 163, 734
Ppu21I YACGTR 1 cut(s) 998
PshAI GACNNNNGTC 1 cut(s) 348
Psp6I CCWGG 3 cut(s) 66, 292, 948
PspGI CCWGG 3 cut(s) 66, 292, 948
PspN4I GGNNCC 2 cut(s) 818, 909
PspPI GGNCC 3 cut(s) 161, 816, 907
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 2 cut(s) 595, 653
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 3 cut(s) 77, 686, 761
SatI GCNGC 3 cut(s) 72, 811, 992
Sau3AI GATC 5 cut(s) 223, 464, 595, 653, 862
Sau96I GGNCC 3 cut(s) 161, 816, 907
SchI GAGTC 2 cut(s) 164, 734
ScrFI CCNGG 3 cut(s) 68, 294, 950
SexAI ACCWGGT 1 cut(s) 948
SfaNI GCATC 2 cut(s) 418, 737
Sfr274I CTCGAG 1 cut(s) 599
SinI GGWCC 2 cut(s) 161, 816
SlaI CTCGAG 1 cut(s) 599
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 599, 685
SmoI CTYRAG 2 cut(s) 599, 685
Sse9I AATT 8 cut(s) 6, 109, 216, 336, 516, 702, 714, 791
SspMI CTAG 3 cut(s) 180, 588, 830
StyD4I CCNGG 3 cut(s) 66, 292, 948
TaiI ACGT 2 cut(s) 826, 1000
TaqI TCGA 3 cut(s) 600, 647, 718
TasI AATT 8 cut(s) 6, 109, 216, 336, 516, 702, 714, 791
TfiI GAWTC 3 cut(s) 138, 289, 326
Tru1I TTAA 3 cut(s) 77, 686, 761
Tru9I TTAA 3 cut(s) 77, 686, 761
TscAI CASTG 2 cut(s) 256, 944
TseFI GTSAC 2 cut(s) 366, 843
TseI GCWGC 3 cut(s) 71, 810, 991
Tsp45I GTSAC 2 cut(s) 366, 843
TspDTI ATGAA 5 cut(s) 270, 559, 622, 714, 1001
TspRI CASTG 2 cut(s) 256, 944
Van91I CCANNNNNTGG 1 cut(s) 299
Vha464I CTTAAG 1 cut(s) 685
VpaK11BI GGWCC 2 cut(s) 161, 816
XapI RAATTY 2 cut(s) 109, 714
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XhoI CTCGAG 1 cut(s) 599
XmnI GAANNNNTTC 2 cut(s) 549, 978
XspI CTAG 3 cut(s) 180, 588, 830
Zsp2I ATGCAT 1 cut(s) 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.