MD02G1318500.v1.1

Protein N-terminal asparagine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005345
Physical Location & Seq
Reverse (-)
37287084 .. 37291268
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1318500.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGATCTTTGTTGATGGGCATCTCTCTTCCACAGACTCTTCTGCATCGCCTCAGGGACGGGATATTCTGGTTGCTCTATTGGAACACCCTGCTTTGGTAGAAGCCTCAGCTTCGTTCAAGGCTATCCCGGAGAGGAGGTTCTCTGTACGTGAAGAGTCAGTTATGGAGGCAACGCCACCCGGTAAATGGGTTTATTTGTTCCAGAGAGAATATGCAACTGTTGATCCGGCATTTGTGGATTTTGTTGGAACTGATGAAGCAACGACCTGTATCGGTGTGGCCATTCGGAATCCGAGAAATGGAATGACCTCGGTTGCTCATATGGATTCTCCAAACATTGTGGATATGGGTCTGTCCCAGATGTTGTCACTCCTCGTTGACCATAACAAGGACATGGAGTTAGATGTGCATCTAGTCGGTGGATTTGAAGATGTTTCAACTAATCATGGTAACTACAACACTACATCAGAAAGTCAGGAAAACATGGATGGTTATTCCTTTCCTTTAGGTGCCAAAATTGTTGAAACTTTATGGAAGAGACCGGAGAAATTCCATATACGTACTCTCTGCATTCTTGGGCATAACACTAGAAGGGATCCCGAGGGAAACGCGTACCCAAACTTCAATGGGTTCATGGTAGGAACTTCCAACGGATCTATTACCCCAGCTATCTTTGACAAAACCTCAAGATGTCCTGATGAAGTTGTCAGGAGAATTCGAGTTTCTGTATCATACAAGGACCCTAGTTGGAAGGGAAAGTTACTGGAAACATATGATACTCAAACTGATCGATTCAAGATAGCTCCATGTTGCTGGACACTGCAGCAGTTACACTTTGCTTTATCATTACAACGTTATTCTGATTCAGAAATCCTCATCATGTGTTCTACTTCACCTTCTGCTGAGAACCCAGATTTTGTGGCGAATATGAGAAGGCATTGGGACTACCTAATCAAACACCCAGATTGGAGAGAAACCTTTCCTATGAAGCAGCCACGCATATTTGAGAGGACTGCCGAAGGAGGCTGGAGAAAGCCACAAGGTGCTGATACATTAATCAGTAGGAGTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
N_Asn_amidohyd

Protein Analysis

358

Amino Acids

40.38

Weight (kDa)

5.79

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
N_Asn_amidohyd PF14736 57 - 344 1.1e-84 Protein N-terminal asparagine amidohydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 509
AccII CGCG 1 cut(s) 611
AclI AACGTT 1 cut(s) 853
AclWI GGATC 4 cut(s) 218, 590, 603, 661
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 548, 714
AdeI CACNNNGTG 1 cut(s) 1043
AfaI GTAC 3 cut(s) 147, 562, 614
AfiI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
AflIII ACRYGT 1 cut(s) 609
AgsI TTSAA 6 cut(s) 118, 428, 438, 524, 625, 796
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 3 cut(s) 110, 668, 803
AluI AGCT 3 cut(s) 110, 668, 803
Alw26I GTCTC 1 cut(s) 532
AlwI GGATC 4 cut(s) 218, 590, 603, 661
Ama87I CYCGRG 1 cut(s) 599
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 2 cut(s) 823, 991
ApoI RAATTY 2 cut(s) 548, 714
AseI ATTAAT 1 cut(s) 1055
Asp700I GAANNNNTTC 1 cut(s) 978
AspS9I GGNCC 1 cut(s) 739
AsuC2I CCSGG 2 cut(s) 128, 180
AsuHPI GGTGA 1 cut(s) 885
AvaI CYCGRG 1 cut(s) 599
AvaII GGWCC 1 cut(s) 739
AxyI CCTNAGG 1 cut(s) 51
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 1 cut(s) 595
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 605, 637
BbvCI CCTCAGC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 835, 1003
BccI CCATC 2 cut(s) 8, 482
BcnI CCSGG 2 cut(s) 128, 180
BcoDI GTCTC 1 cut(s) 532
BfaI CTAG 4 cut(s) 413, 588, 744, 1072
BfmI CTRYAG 1 cut(s) 821
BisI GCNGC 2 cut(s) 824, 992
BlsI GCNGC 2 cut(s) 825, 993
Bme1390I CCNGG 2 cut(s) 128, 180
Bme18I GGWCC 1 cut(s) 739
BmeT110I CYCGRG 1 cut(s) 599
BmgT120I GGNCC 1 cut(s) 739
BmiI GGNNCC 3 cut(s) 511, 597, 741
BmrFI CCNGG 2 cut(s) 128, 180
BmsI GCATC 3 cut(s) 28, 53, 418
BpmI CTGGAG 1 cut(s) 1048
Bpu10I CCTNAGC 1 cut(s) 106
BpuEI CTTGAG 1 cut(s) 670
BpuMI CCSGG 2 cut(s) 128, 180
Bsa29I ATCGAT 1 cut(s) 790
BsaAI YACGTR 2 cut(s) 149, 560
BsaBI GATNNNNATC 2 cut(s) 18, 408
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 2 cut(s) 309, 600
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
Bse1I ACTGG 1 cut(s) 768
Bse21I CCTNAGG 1 cut(s) 51
Bse8I GATNNNNATC 2 cut(s) 18, 408
BseCI ATCGAT 1 cut(s) 790
BseDI CCNNGG 2 cut(s) 309, 600
BseGI GGATG 1 cut(s) 493
BseJI GATNNNNATC 2 cut(s) 18, 408
BseLI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
BseMII CTCAG 3 cut(s) 65, 120, 894
BseNI ACTGG 1 cut(s) 768
BseRI GAGGAG 2 cut(s) 148, 362
BseXI GCAGC 2 cut(s) 835, 1003
BseYI CCCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 611
BshFI GGCC 1 cut(s) 281
BshNI GGYRCC 1 cut(s) 509
BshVI ATCGAT 1 cut(s) 790
BsiHKCI CYCGRG 1 cut(s) 599
BsiSI CCGG 4 cut(s) 128, 180, 227, 542
BslFI GGGAC 3 cut(s) 69, 340, 956
BslI CCNNNNNNNGG 4 cut(s) 94, 186, 299, 388
BsmAI GTCTC 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 69, 340, 956
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 1 cut(s) 281
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 599
Bsp143I GATC 5 cut(s) 3, 223, 595, 653, 787
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 64, 119, 895
BspDI ATCGAT 1 cut(s) 790
BspFNI CGCG 1 cut(s) 611
BspLI GGNNCC 3 cut(s) 511, 597, 741
BspMAI CTGCAG 1 cut(s) 825
BspPI GGATC 4 cut(s) 218, 590, 603, 661
BspT107I GGYRCC 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 532
BsrI ACTGG 1 cut(s) 768
BssECI CCNNGG 2 cut(s) 309, 600
BssMI GATC 5 cut(s) 3, 223, 595, 653, 787
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 4 cut(s) 31, 43, 147, 530
BstBAI YACGTR 2 cut(s) 149, 560
BstDEI CTNAG 3 cut(s) 51, 106, 903
BstF5I GGATG 1 cut(s) 493
BstFNI CGCG 1 cut(s) 611
BstKTI GATC 5 cut(s) 6, 226, 598, 656, 790
BstMAI GTCTC 1 cut(s) 532
BstMBI GATC 5 cut(s) 3, 223, 595, 653, 787
BstSCI CCNGG 2 cut(s) 126, 178
BstSFI CTRYAG 1 cut(s) 821
BstSNI TACGTA 1 cut(s) 560
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 2 cut(s) 835, 1003
BstX2I RGATCY 2 cut(s) 595, 653
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 2 cut(s) 595, 653
Bsu15I ATCGAT 1 cut(s) 790
Bsu36I CCTNAGG 1 cut(s) 51
BsuRI GGCC 1 cut(s) 281
BsuTUI ATCGAT 1 cut(s) 790
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 1 cut(s) 493
BtsI GCAGTG 1 cut(s) 818
BtsIMutI CAGTG 1 cut(s) 818
Cfr13I GGNCC 1 cut(s) 739
ClaI ATCGAT 1 cut(s) 790
Csp6I GTAC 3 cut(s) 146, 561, 613
CspCI CAANNNNNGTGG 2 cut(s) 321, 356
CviAII CATG 6 cut(s) 394, 446, 484, 634, 807, 880
CviJI RGCY 9 cut(s) 104, 110, 122, 281, 668, 803, 994, 1026, 1036
CviKI_1 RGCY 9 cut(s) 104, 110, 122, 281, 668, 803, 994, 1026, 1036
CviQI GTAC 3 cut(s) 146, 561, 613
DdeI CTNAG 3 cut(s) 51, 106, 903
DpnI GATC 5 cut(s) 5, 225, 597, 655, 789
DpnII GATC 5 cut(s) 3, 223, 595, 653, 787
DraIII CACNNNGTG 1 cut(s) 1043
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 4 cut(s) 31, 43, 147, 530
EarI CTCTTC 4 cut(s) 31, 43, 147, 530
Eco105I TACGTA 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 739
Eco81I CCTNAGG 1 cut(s) 51
Eco88I CYCGRG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 739
EcoRI GAATTC 1 cut(s) 714
FaeI CATG 6 cut(s) 397, 449, 487, 637, 810, 883
FaqI GGGAC 3 cut(s) 69, 340, 956
FatI CATG 6 cut(s) 393, 445, 483, 633, 806, 879
FauNDI CATATG 2 cut(s) 321, 772
Fnu4HI GCNGC 2 cut(s) 824, 992
FokI GGATG 1 cut(s) 500
Fsp4HI GCNGC 2 cut(s) 824, 992
FspBI CTAG 4 cut(s) 413, 588, 744, 1072
GluI GCNGC 2 cut(s) 824, 992
GsaI CCCAGC 1 cut(s) 668
GsuI CTGGAG 1 cut(s) 1048
HaeIII GGCC 1 cut(s) 281
HapII CCGG 4 cut(s) 128, 180, 227, 542
Hin1II CATG 6 cut(s) 397, 449, 487, 637, 810, 883
HincII GTYRAC 1 cut(s) 379
HindII GTYRAC 1 cut(s) 379
HinfI GANTC 6 cut(s) 35, 155, 289, 326, 792, 863
HpaII CCGG 4 cut(s) 128, 180, 227, 542
HphI GGTGA 1 cut(s) 885
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 5 cut(s) 288, 294, 469, 862, 868
Hpy188III TCNNGA 7 cut(s) 202, 476, 599, 687, 695, 709, 796
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 5 cut(s) 585, 745, 906, 927, 1013
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 3 cut(s) 148, 559, 853
HpyCH4V TGCA 5 cut(s) 44, 215, 409, 570, 823
HpyF3I CTNAG 3 cut(s) 51, 106, 903
HpySE526I ACGT 3 cut(s) 148, 559, 853
Hsp92II CATG 6 cut(s) 397, 449, 487, 637, 810, 883
Kzo9I GATC 5 cut(s) 3, 223, 595, 653, 787
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 2 cut(s) 835, 1003
LweI GCATC 3 cut(s) 28, 53, 418
MaeI CTAG 4 cut(s) 413, 588, 744, 1072
MaeII ACGT 3 cut(s) 148, 559, 853
MaeIII GTNAC 4 cut(s) 366, 449, 759, 828
MalI GATC 5 cut(s) 5, 225, 597, 655, 789
MboI GATC 5 cut(s) 3, 223, 595, 653, 787
MboII GAAGA 5 cut(s) 18, 30, 164, 440, 547
MflI RGATCY 2 cut(s) 595, 653
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 3 cut(s) 516, 548, 714
MluI ACGCGT 1 cut(s) 609
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 2 cut(s) 29, 164
MmeI TCCRAC 3 cut(s) 226, 672, 728
Mox20I TGGCCA 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 978
MscI TGGCCA 1 cut(s) 281
MseI TTAA 1 cut(s) 1055
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 4 cut(s) 128, 180, 227, 542
MspR9I CCNGG 2 cut(s) 128, 180
Mva1269I GAATGC 1 cut(s) 570
MvnI CGCG 1 cut(s) 611
NciI CCSGG 2 cut(s) 128, 180
NdeI CATATG 2 cut(s) 321, 772
NdeII GATC 5 cut(s) 3, 223, 595, 653, 787
NlaIII CATG 6 cut(s) 397, 449, 487, 637, 810, 883
NlaIV GGNNCC 3 cut(s) 511, 597, 741
NmuCI GTSAC 1 cut(s) 366
PctI GAATGC 1 cut(s) 570
PdmI GAANNNNTTC 1 cut(s) 978
PfeI GAWTC 4 cut(s) 289, 326, 792, 863
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 2 cut(s) 825, 993
PleI GAGTC 2 cut(s) 29, 163
PpsI GAGTC 2 cut(s) 29, 163
Ppu21I YACGTR 2 cut(s) 149, 560
PpuMI RGGWCCY 1 cut(s) 739
PshBI ATTAAT 1 cut(s) 1055
Psp1406I AACGTT 1 cut(s) 853
Psp5II RGGWCCY 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 664
PspN4I GGNNCC 3 cut(s) 511, 597, 741
PspPI GGNCC 1 cut(s) 739
PspPPI RGGWCCY 1 cut(s) 739
PstI CTGCAG 1 cut(s) 825
PsuI RGATCY 2 cut(s) 595, 653
RsaI GTAC 3 cut(s) 147, 562, 614
RsaNI GTAC 3 cut(s) 146, 561, 613
SaqAI TTAA 1 cut(s) 1055
SatI GCNGC 2 cut(s) 824, 992
Sau3AI GATC 5 cut(s) 3, 223, 595, 653, 787
Sau96I GGNCC 1 cut(s) 739
SchI GAGTC 2 cut(s) 29, 164
ScrFI CCNGG 2 cut(s) 128, 180
SfaNI GCATC 3 cut(s) 28, 53, 418
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 1 cut(s) 739
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
SnaBI TACGTA 1 cut(s) 560
Sse9I AATT 3 cut(s) 516, 548, 714
SspMI CTAG 4 cut(s) 413, 588, 744, 1072
StyD4I CCNGG 2 cut(s) 126, 178
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 3 cut(s) 151, 562, 856
TaqI TCGA 2 cut(s) 718, 790
TasI AATT 3 cut(s) 516, 548, 714
TfiI GAWTC 4 cut(s) 289, 326, 792, 863
Tru1I TTAA 1 cut(s) 1055
Tru9I TTAA 1 cut(s) 1055
TscAI CASTG 1 cut(s) 825
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 2 cut(s) 823, 991
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 4 cut(s) 270, 622, 714, 1001
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 825
VpaK11BI GGWCC 1 cut(s) 739
VspI ATTAAT 1 cut(s) 1055
XapI RAATTY 2 cut(s) 548, 714
XcmI CCANNNNNNNNNTGG 1 cut(s) 183
XmnI GAANNNNTTC 1 cut(s) 978
XspI CTAG 4 cut(s) 413, 588, 744, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.