AT2G45530

RING-variant domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004996
Physical Location & Seq
Forward (+)
18756471 .. 18757513
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45530.1

Sequence Viewer

Length: 723 bp
ATGCAAAGTAGCTCCAGCTCCGGTGGTGACGACCTTGAAAAGCAGCAGCAGCAGCAGCAGAAGGACAAGTCTCCCCAGAAGCAGAGCGAGTCAGCCAATGAATCGAACCACCTCACAATCGTTGTCTGCAATGGAGATTCTAGTCGGGAAGAATTGGTGGGTCAGATCCCACCGGAAAAGGAGGTTAGCTTATCCAGAAACGGAAGCTCGCACGAGCAGTGCAGGGTTTGTCTACAAGACAAAGAAGAGGTTTTGATTGAACTAGGTTGTCAATGTCGTGGTGGTCTTGCTAAAGCTCACAGGTCTTGTATAGATGCTTGGTTTCGTACTAAAGGCTCTAACCAGTGTGAGATCTGTCAAGTTGTTGCTGTTAATGTCACACCTCCTGAGACTCAACCAACTACAAACTATTGGGTTTGGAGGATTGATCCTAGCTATAGACAAGAAGAGCGTGAGAGAGGATGCTTTAGTCCGCTTTGGGTTGCATTTTCGATTCTGATCGGTGGTTTAATGCTTGATGTGTTGATATCTATCACACTTGGGGTCTCTGCGCTTCCGGTGAACATCATTATTGGAGTCATAGTTGTGCTTGGGCTAGGAACAGCTCTAAGGCTGACTTTGGAGTTCTGTTATGAGTGGAGCTTGAGAAGAGCGGTTCACAGGGCGGTGCAGAGGGCGGAAGCGAACAACTTCAGTAATATTGCGTACCCACCTGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

240

Amino Acids

26.63

Weight (kDa)

5.65

Isoelectric Point (pI)

56.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 74 - 119 1.5e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 653
AccI GTMKAC 1 cut(s) 232
AciI CCGC 4 cut(s) 473, 653, 665, 677
AclWI GGATC 2 cut(s) 160, 422
AcuI CTGAAG 1 cut(s) 676
AfaI GTAC 2 cut(s) 328, 707
AgsI TTSAA 2 cut(s) 38, 260
AluBI AGCT 8 cut(s) 12, 18, 189, 207, 296, 435, 605, 642
AluI AGCT 8 cut(s) 12, 18, 189, 207, 296, 435, 605, 642
Alw26I GTCTC 3 cut(s) 75, 383, 550
AlwI GGATC 2 cut(s) 160, 422
ApeKI GCWGC 5 cut(s) 43, 46, 49, 52, 55
Asp700I GAANNNNTTC 1 cut(s) 689
AspLEI GCGC 1 cut(s) 553
AsuHPI GGTGA 2 cut(s) 38, 571
BauI CACGAG 1 cut(s) 212
BbvI GCAGC 5 cut(s) 55, 58, 61, 64, 67
BcoDI GTCTC 3 cut(s) 75, 383, 550
BfaI CTAG 4 cut(s) 141, 263, 432, 596
BfmI CTRYAG 1 cut(s) 436
BglII AGATCT 1 cut(s) 351
BisI GCNGC 5 cut(s) 44, 47, 50, 53, 56
BlsI GCNGC 5 cut(s) 45, 48, 51, 54, 57
BmsI GCATC 2 cut(s) 304, 452
BpuEI CTTGAG 1 cut(s) 664
BsaBI GATNNNNATC 2 cut(s) 497, 530
BsaI GGTCTC 1 cut(s) 550
BsaWI WCCGGW 3 cut(s) 20, 172, 556
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 2 cut(s) 497, 530
BseGI GGATG 1 cut(s) 467
BseJI GATNNNNATC 2 cut(s) 497, 530
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 1 cut(s) 343
BseXI GCAGC 5 cut(s) 55, 58, 61, 64, 67
BsgI GTGCAG 2 cut(s) 241, 689
BsiSI CCGG 3 cut(s) 21, 173, 557
BsmAI GTCTC 3 cut(s) 75, 383, 550
Bso31I GGTCTC 1 cut(s) 550
Bsp143I GATC 4 cut(s) 165, 351, 427, 498
BspACI CCGC 4 cut(s) 473, 653, 665, 677
BspCNI CTCAG 1 cut(s) 379
BspPI GGATC 2 cut(s) 160, 422
BspQI GCTCTTC 2 cut(s) 441, 643
BspTNI GGTCTC 1 cut(s) 550
BsrBI CCGCTC 1 cut(s) 653
BsrDI GCAATG 1 cut(s) 136
BsrI ACTGG 1 cut(s) 343
BssMI GATC 4 cut(s) 165, 351, 427, 498
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst6I CTCTTC 3 cut(s) 240, 441, 643
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 2 cut(s) 387, 608
BstF5I GGATG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 553
BstKTI GATC 4 cut(s) 168, 354, 430, 501
BstMAI GTCTC 3 cut(s) 75, 383, 550
BstMBI GATC 4 cut(s) 165, 351, 427, 498
BstMWI GCNNNNNNNGC 3 cut(s) 49, 52, 55
BstSFI CTRYAG 1 cut(s) 436
BstV1I GCAGC 5 cut(s) 55, 58, 61, 64, 67
BstX2I RGATCY 2 cut(s) 165, 351
BstYI RGATCY 2 cut(s) 165, 351
BtsCI GGATG 1 cut(s) 467
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 224, 350
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 327, 706
CspCI CAANNNNNGTGG 1 cut(s) 699
CviQI GTAC 2 cut(s) 327, 706
DdeI CTNAG 2 cut(s) 387, 608
DpnI GATC 4 cut(s) 167, 353, 429, 500
DpnII GATC 4 cut(s) 165, 351, 427, 498
Eam1104I CTCTTC 3 cut(s) 240, 441, 643
EarI CTCTTC 3 cut(s) 240, 441, 643
EciI GGCGGA 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 550
Eco32I GATATC 1 cut(s) 528
Eco57I CTGAAG 1 cut(s) 676
EcoRV GATATC 1 cut(s) 528
FaiI YATR 4 cut(s) 311, 438, 581, 633
FalI AAGNNNNNCTT 2 cut(s) 601, 633
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 5 cut(s) 44, 47, 50, 53, 56
FokI GGATG 1 cut(s) 474
Fsp4HI GCNGC 5 cut(s) 44, 47, 50, 53, 56
FspBI CTAG 4 cut(s) 141, 263, 432, 596
GlaI GCGC 1 cut(s) 552
GluI GCNGC 5 cut(s) 44, 47, 50, 53, 56
HapII CCGG 3 cut(s) 21, 173, 557
HhaI GCGC 1 cut(s) 553
Hin6I GCGC 1 cut(s) 551
HinP1I GCGC 1 cut(s) 551
HinfI GANTC 6 cut(s) 89, 101, 137, 391, 493, 576
HpaII CCGG 3 cut(s) 21, 173, 557
HphI GGTGA 2 cut(s) 38, 571
Hpy166II GTNNAC 3 cut(s) 233, 562, 658
Hpy188I TCNGA 2 cut(s) 165, 498
Hpy188III TCNNGA 3 cut(s) 146, 195, 386
Hpy8I GTNNAC 3 cut(s) 233, 562, 658
HpyAV CCTTC 1 cut(s) 55
HpyCH4V TGCA 5 cut(s) 4, 129, 222, 485, 670
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 52, 55
HpyF3I CTNAG 2 cut(s) 387, 608
HspAI GCGC 1 cut(s) 551
Kzo9I GATC 4 cut(s) 165, 351, 427, 498
LguI GCTCTTC 2 cut(s) 441, 643
LmnI GCTCC 3 cut(s) 17, 23, 639
Lsp1109I GCAGC 5 cut(s) 55, 58, 61, 64, 67
LweI GCATC 2 cut(s) 304, 452
MaeI CTAG 4 cut(s) 141, 263, 432, 596
MaeIII GTNAC 2 cut(s) 26, 376
MalI GATC 4 cut(s) 167, 353, 429, 500
MbiI CCGCTC 1 cut(s) 653
MboI GATC 4 cut(s) 165, 351, 427, 498
MboII GAAGA 4 cut(s) 161, 257, 458, 660
MflI RGATCY 2 cut(s) 165, 351
MluCI AATT 1 cut(s) 152
MlyI GAGTC 3 cut(s) 98, 385, 585
MnlI CCTC 7 cut(s) 122, 175, 241, 393, 414, 452, 666
MroXI GAANNNNTTC 1 cut(s) 689
MseI TTAA 2 cut(s) 372, 509
MslI CAYNNNNRTG 1 cut(s) 584
MspI CCGG 3 cut(s) 21, 173, 557
MwoI GCNNNNNNNGC 3 cut(s) 49, 52, 55
NdeII GATC 4 cut(s) 165, 351, 427, 498
NmuCI GTSAC 2 cut(s) 26, 376
PciSI GCTCTTC 2 cut(s) 441, 643
PdmI GAANNNNTTC 1 cut(s) 689
PfeI GAWTC 3 cut(s) 101, 137, 493
PkrI GCNGC 5 cut(s) 45, 48, 51, 54, 57
PleI GAGTC 3 cut(s) 97, 385, 584
PpsI GAGTC 3 cut(s) 97, 385, 584
PsuI RGATCY 2 cut(s) 165, 351
RsaI GTAC 2 cut(s) 328, 707
RsaNI GTAC 2 cut(s) 327, 706
RseI CAYNNNNRTG 1 cut(s) 584
SapI GCTCTTC 2 cut(s) 441, 643
SaqAI TTAA 2 cut(s) 372, 509
SatI GCNGC 5 cut(s) 44, 47, 50, 53, 56
Sau3AI GATC 4 cut(s) 165, 351, 427, 498
SchI GAGTC 3 cut(s) 98, 385, 585
SfaNI GCATC 2 cut(s) 304, 452
SfcI CTRYAG 1 cut(s) 436
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
Sse9I AATT 1 cut(s) 152
SsiI CCGC 4 cut(s) 473, 653, 665, 677
SspI AATATT 1 cut(s) 700
SspMI CTAG 4 cut(s) 141, 263, 432, 596
TaqI TCGA 2 cut(s) 104, 491
TasI AATT 1 cut(s) 152
TfiI GAWTC 3 cut(s) 101, 137, 493
Tru1I TTAA 2 cut(s) 372, 509
Tru9I TTAA 2 cut(s) 372, 509
TscAI CASTG 2 cut(s) 224, 350
TseFI GTSAC 2 cut(s) 26, 376
TseI GCWGC 5 cut(s) 43, 46, 49, 52, 55
Tsp45I GTSAC 2 cut(s) 26, 376
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 2 cut(s) 224, 350
XmiI GTMKAC 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 689
XspI CTAG 4 cut(s) 141, 263, 432, 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.