RchiOBHm_Chr7g0187881

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004996
Physical Location & Seq
Reverse (-)
7598828 .. 7601018
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16775

Sequence Viewer

Length: 726 bp
ATGAGCAATGACACGGAGAGCTCAAATTGCGAGCCTCCTGACCTTGAGAAGCAGCAGCACCGTGACGGCGGCGCTGAGGCGGTCACCTCAATTCCCGATTCTTCTGCCGTTGAGGATACGGTGCCGCCGCTGCTGAACGTCGTCGTTTCCGAACCACCACCGGCCCCGCCCGCCGCCGCCAATTCGTCCAAGGATGTTAAGGGACAGCATTCCAGAAACTCCAGCGCTCATGAACAATGCAGGGTGTGTCAGCAGGATAAAGATGAAGCTTTGATTGATCTCGGATGCCATTGTCGAGGTGAACTTGCGAAAGCGCACAGATCATGTATAGACACTTGGTTTCAATCCAAAGGATCAAACAAATGCGAGATTTGCCAAGAGGTAGCTGCGAATGTGTCATCTCCAGAATCCCAGCCAACTACAAATTATTGGGTTTGGAGAATTGAAACAACCTATCGATCGCAAAACCCAGACCAGGGCTGTATGAGCCCACTTTGGGTGGCATTCTCAATCCTCATTGGTGGTCTGCTGTTGGACGTGCTAATATCTGCCACCCTAGGCATCTCTGCAGTTCCCATTAACATTATCATCGGTGTTATTGTGGTCCTTGGGCTTGGAACTGTACTGAGACTTGCCTTGGCGTTTTGCCATGAGTGGGGTTCGAGGAGAGTTGTGCAGCGGGTAGACACACAGAGAGTGCCTCTTGGCTACCATCCTGGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

241

Amino Acids

26.09

Weight (kDa)

5.33

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 80 - 125 6.2e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 684
AciI CCGC 9 cut(s) 69, 80, 125, 128, 167, 171, 174, 177, 679
AclWI GGATC 1 cut(s) 361
AfaI GTAC 1 cut(s) 624
AfeI AGCGCT 1 cut(s) 226
AfiI CCNNNNNNNGG 4 cut(s) 475, 476, 496, 655
AgsI TTSAA 2 cut(s) 344, 446
AjiI CACGTC 1 cut(s) 538
AjnI CCWGG 2 cut(s) 474, 715
AluBI AGCT 3 cut(s) 21, 269, 386
AluI AGCT 3 cut(s) 21, 269, 386
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 622
AlwI GGATC 1 cut(s) 361
Aor51HI AGCGCT 1 cut(s) 226
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 5 cut(s) 52, 55, 130, 386, 676
AspA2I CCTAGG 1 cut(s) 556
AspLEI GCGC 3 cut(s) 74, 227, 316
AspS9I GGNCC 2 cut(s) 163, 604
AsuHPI GGTGA 2 cut(s) 76, 311
AvaII GGWCC 1 cut(s) 604
AvrII CCTAGG 1 cut(s) 556
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 2 cut(s) 23, 491
Bbv12I GWGCWC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 5 cut(s) 64, 67, 117, 373, 688
BccI CCATC 1 cut(s) 720
BceAI ACGGC 2 cut(s) 82, 92
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 476, 717
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 622
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 2 cut(s) 75, 228
BfuI GTATCC 1 cut(s) 109
BlnI CCTAGG 1 cut(s) 556
Bme1390I CCNGG 2 cut(s) 476, 717
Bme18I GGWCC 1 cut(s) 604
BmgBI CACGTC 1 cut(s) 538
BmgT120I GGNCC 2 cut(s) 163, 604
BmiI GGNNCC 2 cut(s) 123, 165
BmrFI CCNGG 2 cut(s) 476, 717
BmsI GCATC 2 cut(s) 275, 570
BplI GAGNNNNNCTC 2 cut(s) 685, 717
BpmI CTGGAG 2 cut(s) 205, 387
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 457
BsaJI CCNNGG 5 cut(s) 189, 475, 556, 607, 636
Bsc4I CCNNNNNNNGG 4 cut(s) 475, 476, 496, 655
Bse118I RCCGGY 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 2 cut(s) 476, 717
BseCI ATCGAT 1 cut(s) 457
BseDI CCNNGG 5 cut(s) 189, 475, 556, 607, 636
BseGI GGATG 3 cut(s) 199, 290, 712
BseLI CCNNNNNNNGG 4 cut(s) 475, 476, 496, 655
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 66, 617
BseRI GAGGAG 1 cut(s) 679
BseXI GCAGC 5 cut(s) 64, 67, 117, 373, 688
BseYI CCCAGC 1 cut(s) 411
BsgI GTGCAG 1 cut(s) 695
Bsh1285I CGRYCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 457
BsiEI CGRYCG 1 cut(s) 461
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 4 cut(s) 475, 476, 496, 655
BsmAI GTCTC 1 cut(s) 622
BsmFI GGGAC 1 cut(s) 216
BsmI GAATGC 2 cut(s) 208, 503
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 23, 491
Bsp143I GATC 4 cut(s) 277, 320, 353, 458
BspACI CCGC 9 cut(s) 69, 80, 125, 128, 167, 171, 174, 177, 679
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 2 cut(s) 67, 618
BspDI ATCGAT 1 cut(s) 457
BspHI TCATGA 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 123, 165
BspMAI CTGCAG 1 cut(s) 571
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 5 cut(s) 189, 475, 556, 607, 636
BssMI GATC 4 cut(s) 277, 320, 353, 458
BssT1I CCWWGG 4 cut(s) 189, 556, 607, 636
Bst2UI CCWGG 2 cut(s) 476, 717
Bst4CI ACNGT 3 cut(s) 62, 121, 622
BstC8I GCNNGC 2 cut(s) 32, 171
BstDEI CTNAG 2 cut(s) 75, 626
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 3 cut(s) 199, 290, 712
BstH2I RGCGCY 2 cut(s) 75, 228
BstHHI GCGC 3 cut(s) 74, 227, 316
BstKTI GATC 4 cut(s) 280, 323, 356, 461
BstMAI GTCTC 1 cut(s) 622
BstMBI GATC 4 cut(s) 277, 320, 353, 458
BstMCI CGRYCG 1 cut(s) 461
BstMWI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 372, 486
BstNI CCWGG 2 cut(s) 476, 717
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 474, 715
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 5 cut(s) 64, 67, 117, 373, 688
Bsu15I ATCGAT 1 cut(s) 457
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 164
BsuTUI ATCGAT 1 cut(s) 457
BtrI CACGTC 1 cut(s) 538
BtsCI GGATG 3 cut(s) 199, 290, 712
Cac8I GCNNGC 2 cut(s) 32, 171
CciI TCATGA 1 cut(s) 229
CfoI GCGC 3 cut(s) 74, 227, 316
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 163, 604
ClaI ATCGAT 1 cut(s) 457
Csp6I GTAC 1 cut(s) 623
CviAII CATG 3 cut(s) 230, 324, 650
CviQI GTAC 1 cut(s) 623
DdeI CTNAG 2 cut(s) 75, 626
DpnI GATC 4 cut(s) 279, 322, 355, 460
DpnII GATC 4 cut(s) 277, 320, 353, 458
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 4 cut(s) 189, 556, 607, 636
Eco24I GRGCYC 2 cut(s) 23, 491
Eco47I GGWCC 1 cut(s) 604
Eco47III AGCGCT 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 21
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 474, 715
EcoT14I CCWWGG 4 cut(s) 189, 556, 607, 636
EcoT38I GRGCYC 2 cut(s) 23, 491
ErhI CCWWGG 4 cut(s) 189, 556, 607, 636
FaeI CATG 3 cut(s) 233, 327, 653
FaiI YATR 5 cut(s) 231, 325, 329, 485, 651
FaqI GGGAC 1 cut(s) 216
FatI CATG 3 cut(s) 229, 323, 649
FauI CCCGC 3 cut(s) 174, 178, 672
FblI GTMKAC 1 cut(s) 684
FokI GGATG 3 cut(s) 206, 297, 699
FriOI GRGCYC 2 cut(s) 23, 491
FspBI CTAG 1 cut(s) 557
GlaI GCGC 3 cut(s) 73, 226, 315
GsaI CCCAGC 1 cut(s) 415
GsuI CTGGAG 2 cut(s) 205, 387
HaeII RGCGCY 2 cut(s) 75, 228
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 161
HhaI GCGC 3 cut(s) 74, 227, 316
Hin1II CATG 3 cut(s) 233, 327, 653
Hin6I GCGC 3 cut(s) 72, 225, 314
HinP1I GCGC 3 cut(s) 72, 225, 314
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 2 cut(s) 98, 407
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 76, 311
Hpy166II GTNNAC 2 cut(s) 302, 685
Hpy188I TCNGA 2 cut(s) 151, 284
Hpy188III TCNNGA 5 cut(s) 38, 95, 213, 230, 404
Hpy8I GTNNAC 2 cut(s) 302, 685
Hpy99I CGWCG 2 cut(s) 143, 146
HpyCH4III ACNGT 3 cut(s) 62, 121, 622
HpyCH4IV ACGT 2 cut(s) 138, 537
HpyCH4V TGCA 3 cut(s) 240, 569, 676
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 372, 486
HpyF3I CTNAG 2 cut(s) 75, 626
HpySE526I ACGT 2 cut(s) 138, 537
Hsp92II CATG 3 cut(s) 233, 327, 653
HspAI GCGC 3 cut(s) 72, 225, 314
Kzo9I GATC 4 cut(s) 277, 320, 353, 458
Lsp1109I GCAGC 5 cut(s) 64, 67, 117, 373, 688
LweI GCATC 2 cut(s) 275, 570
MaeI CTAG 1 cut(s) 557
MaeII ACGT 2 cut(s) 138, 537
MaeIII GTNAC 2 cut(s) 62, 82
MalI GATC 4 cut(s) 279, 322, 355, 460
MboI GATC 4 cut(s) 277, 320, 353, 458
MboII GAAGA 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 23, 491
MluCI AATT 5 cut(s) 25, 90, 181, 424, 441
MmeI TCCRAC 1 cut(s) 513
MnlI CCTC 9 cut(s) 45, 70, 97, 106, 290, 373, 524, 657, 711
MseI TTAA 2 cut(s) 198, 579
MspA1I CMGCKG 2 cut(s) 130, 679
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 476, 717
Mva1269I GAATGC 2 cut(s) 208, 503
MvaI CCWGG 2 cut(s) 476, 717
MwoI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 372, 486
NdeII GATC 4 cut(s) 277, 320, 353, 458
NlaIII CATG 3 cut(s) 233, 327, 653
NlaIV GGNNCC 2 cut(s) 123, 165
NmuCI GTSAC 2 cut(s) 62, 82
PagI TCATGA 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 208, 503
PfeI GAWTC 2 cut(s) 98, 407
Ple19I CGATCG 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 474, 715
PspEI GGTNACC 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 2 cut(s) 474, 715
PspN4I GGNNCC 2 cut(s) 123, 165
PspPI GGNCC 2 cut(s) 163, 604
PstI CTGCAG 1 cut(s) 571
PvuI CGATCG 1 cut(s) 461
RsaI GTAC 1 cut(s) 624
RsaNI GTAC 1 cut(s) 623
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 198, 579
Sau3AI GATC 4 cut(s) 277, 320, 353, 458
Sau96I GGNCC 2 cut(s) 163, 604
ScrFI CCNGG 2 cut(s) 476, 717
SduI GDGCHC 2 cut(s) 23, 491
SfaNI GCATC 2 cut(s) 275, 570
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 1 cut(s) 604
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 25, 90, 181, 424, 441
SsiI CCGC 9 cut(s) 69, 80, 125, 128, 167, 171, 174, 177, 679
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 474, 715
StyI CCWWGG 4 cut(s) 189, 556, 607, 636
TaaI ACNGT 3 cut(s) 62, 121, 622
TaiI ACGT 2 cut(s) 141, 540
TaqI TCGA 3 cut(s) 295, 457, 662
TasI AATT 5 cut(s) 25, 90, 181, 424, 441
TatI WGTACW 1 cut(s) 622
TauI GCSGC 5 cut(s) 72, 127, 130, 176, 179
TfiI GAWTC 2 cut(s) 98, 407
Tru1I TTAA 2 cut(s) 198, 579
Tru9I TTAA 2 cut(s) 198, 579
TseFI GTSAC 2 cut(s) 62, 82
TseI GCWGC 5 cut(s) 52, 55, 130, 386, 676
Tsp45I GTSAC 2 cut(s) 62, 82
TspDTI ATGAA 2 cut(s) 246, 279
TspGWI ACGGA 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 604
XmaJI CCTAGG 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.