Rroxscaffold_3G00266980

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004996
Physical Location & Seq
Forward (+)
60137750 .. 60139698
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00266980.1

Sequence Viewer

Length: 723 bp
ATGAGCAATAACACGGAGAGCTCAAATTGCGAGCCTCCTGACCTTGAGAAGCAGCAGCACCGTGACGGCGGCGCTGAGGCGGTTGCCTCAATTCTCGATTCTTCTGCCGTTGAGGATACGGTGCCGCCGCTGCTGAACGTCGTCGTTTCCCAACCACCACCGGCTCCGCCCGCCGCCAATTCGTCCAAGGAGGTTAAGGGACAGCATTCCAGAAACTCCAGCGCTCATGAACAATGCAGGGTGTGTCAGCAGGATAAAGATGAAGCTTTGATTGATCTCGGATGCCATTGTCGAGGTGAACTTGCGAAAGCGCACAGATCATGTATAGACACTTGGTTTCATTCCAAAGGATCAAACAAATGCGAGATTTGCCAAGAGGTAGCTACGAATGTGTCATCTCCAGAATCCCAGCCAACTACAAATTATTGGGTTTGGAGAATTGAAACAACCTATCGATCGCAAAACCCAGACCAGGGCTGTATGAGCCCACTTTGGGTGGCATTCTCAATCCTCATTGGTGGTCTGCTGTTGGACGTGCTAATATCTGCCACCCTTGGCATCTCTGCAGTTCCCATTAACATTATCATCGGTGTTATTGTGGTCCTTGGGCTTGGAACTGTACTGAGACTTGCCTTGGCGTTTTGCCATGAGTGGGGTTCGAGGAGAGTTGTGCAGCGGGTGGACACACAGAGAGTGCCTCTTGGCTACCATCCTGGTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

240

Amino Acids

26.06

Weight (kDa)

5.73

Isoelectric Point (pI)

54.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 79 - 124 1.5e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 8 cut(s) 69, 80, 125, 128, 167, 171, 174, 676
AclWI GGATC 1 cut(s) 358
AfaI GTAC 1 cut(s) 621
AfeI AGCGCT 1 cut(s) 223
AfiI CCNNNNNNNGG 4 cut(s) 472, 473, 493, 652
AgsI TTSAA 1 cut(s) 443
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 2 cut(s) 471, 712
AluBI AGCT 3 cut(s) 21, 266, 383
AluI AGCT 3 cut(s) 21, 266, 383
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 619
AlwI GGATC 1 cut(s) 358
Aor51HI AGCGCT 1 cut(s) 223
ApeKI GCWGC 4 cut(s) 52, 55, 130, 673
AspLEI GCGC 3 cut(s) 74, 224, 313
AspS9I GGNCC 1 cut(s) 601
AsuHPI GGTGA 1 cut(s) 308
AvaII GGWCC 1 cut(s) 601
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 2 cut(s) 23, 488
Bbv12I GWGCWC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 4 cut(s) 64, 67, 117, 685
BccI CCATC 1 cut(s) 717
BceAI ACGGC 2 cut(s) 82, 92
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 473, 714
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 619
BfmI CTRYAG 2 cut(s) 564, 719
BfoI RGCGCY 2 cut(s) 75, 225
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 674
BlsI GCNGC 8 cut(s) 54, 57, 71, 126, 129, 132, 175, 675
Bme1390I CCNGG 2 cut(s) 473, 714
Bme18I GGWCC 1 cut(s) 601
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 2 cut(s) 123, 165
BmrFI CCNGG 2 cut(s) 473, 714
BmsI GCATC 2 cut(s) 272, 567
BplI GAGNNNNNCTC 2 cut(s) 682, 714
BpmI CTGGAG 2 cut(s) 202, 384
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 454
BsaJI CCNNGG 5 cut(s) 186, 472, 553, 604, 633
Bsc4I CCNNNNNNNGG 4 cut(s) 472, 473, 493, 652
Bse118I RCCGGY 1 cut(s) 160
BseBI CCWGG 2 cut(s) 473, 714
BseCI ATCGAT 1 cut(s) 454
BseDI CCNNGG 5 cut(s) 186, 472, 553, 604, 633
BseGI GGATG 2 cut(s) 287, 709
BseLI CCNNNNNNNGG 4 cut(s) 472, 473, 493, 652
BseMII CTCAG 2 cut(s) 66, 614
BseRI GAGGAG 1 cut(s) 676
BseXI GCAGC 4 cut(s) 64, 67, 117, 685
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 1 cut(s) 692
Bsh1285I CGRYCG 1 cut(s) 458
BshNI GGYRCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 454
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 4 cut(s) 472, 473, 493, 652
BsmAI GTCTC 1 cut(s) 619
BsmFI GGGAC 1 cut(s) 213
BsmI GAATGC 2 cut(s) 205, 500
Bsp1286I GDGCHC 2 cut(s) 23, 488
Bsp143I GATC 4 cut(s) 274, 317, 350, 455
BspACI CCGC 8 cut(s) 69, 80, 125, 128, 167, 171, 174, 676
BspCNI CTCAG 2 cut(s) 67, 615
BspDI ATCGAT 1 cut(s) 454
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 123, 165
BspMAI CTGCAG 1 cut(s) 568
BspPI GGATC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 5 cut(s) 186, 472, 553, 604, 633
BssMI GATC 4 cut(s) 274, 317, 350, 455
BssT1I CCWWGG 4 cut(s) 186, 553, 604, 633
Bst2UI CCWGG 2 cut(s) 473, 714
Bst4CI ACNGT 3 cut(s) 62, 121, 619
BstC8I GCNNGC 2 cut(s) 32, 171
BstDEI CTNAG 2 cut(s) 75, 623
BstF5I GGATG 2 cut(s) 287, 709
BstH2I RGCGCY 2 cut(s) 75, 225
BstHHI GCGC 3 cut(s) 74, 224, 313
BstKTI GATC 4 cut(s) 277, 320, 353, 458
BstMAI GTCTC 1 cut(s) 619
BstMBI GATC 4 cut(s) 274, 317, 350, 455
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 369, 483
BstNI CCWGG 2 cut(s) 473, 714
BstSCI CCNGG 2 cut(s) 471, 712
BstSFI CTRYAG 2 cut(s) 564, 719
BstV1I GCAGC 4 cut(s) 64, 67, 117, 685
Bsu15I ATCGAT 1 cut(s) 454
BsuI GTATCC 1 cut(s) 109
BsuTUI ATCGAT 1 cut(s) 454
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 2 cut(s) 287, 709
Cac8I GCNNGC 2 cut(s) 32, 171
CciI TCATGA 1 cut(s) 226
CfoI GCGC 3 cut(s) 74, 224, 313
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 1 cut(s) 601
ClaI ATCGAT 1 cut(s) 454
Csp6I GTAC 1 cut(s) 620
CviAII CATG 3 cut(s) 227, 321, 647
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 2 cut(s) 75, 623
DpnI GATC 4 cut(s) 276, 319, 352, 457
DpnII GATC 4 cut(s) 274, 317, 350, 455
EciI GGCGGA 1 cut(s) 156
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 4 cut(s) 186, 553, 604, 633
Eco24I GRGCYC 2 cut(s) 23, 488
Eco47I GGWCC 1 cut(s) 601
Eco47III AGCGCT 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 2 cut(s) 471, 712
EcoT14I CCWWGG 4 cut(s) 186, 553, 604, 633
EcoT38I GRGCYC 2 cut(s) 23, 488
ErhI CCWWGG 4 cut(s) 186, 553, 604, 633
FaeI CATG 3 cut(s) 230, 324, 650
FaiI YATR 5 cut(s) 228, 322, 326, 482, 648
FaqI GGGAC 1 cut(s) 213
FatI CATG 3 cut(s) 226, 320, 646
FauI CCCGC 2 cut(s) 178, 669
Fnu4HI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 674
FokI GGATG 2 cut(s) 294, 696
FriOI GRGCYC 2 cut(s) 23, 488
Fsp4HI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 674
GlaI GCGC 3 cut(s) 73, 223, 312
GluI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 674
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 2 cut(s) 202, 384
HaeII RGCGCY 2 cut(s) 75, 225
HapII CCGG 1 cut(s) 161
HhaI GCGC 3 cut(s) 74, 224, 313
Hin1II CATG 3 cut(s) 230, 324, 650
Hin6I GCGC 3 cut(s) 72, 222, 311
HinP1I GCGC 3 cut(s) 72, 222, 311
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 2 cut(s) 98, 404
HpaII CCGG 1 cut(s) 161
HphI GGTGA 1 cut(s) 308
Hpy166II GTNNAC 2 cut(s) 299, 682
Hpy188I TCNGA 1 cut(s) 281
Hpy188III TCNNGA 5 cut(s) 38, 95, 210, 227, 401
Hpy8I GTNNAC 2 cut(s) 299, 682
Hpy99I CGWCG 2 cut(s) 143, 146
HpyCH4III ACNGT 3 cut(s) 62, 121, 619
HpyCH4IV ACGT 2 cut(s) 138, 534
HpyCH4V TGCA 3 cut(s) 237, 566, 673
HpyF10VI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 369, 483
HpyF3I CTNAG 2 cut(s) 75, 623
HpySE526I ACGT 2 cut(s) 138, 534
Hsp92II CATG 3 cut(s) 230, 324, 650
HspAI GCGC 3 cut(s) 72, 222, 311
Kzo9I GATC 4 cut(s) 274, 317, 350, 455
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 4 cut(s) 64, 67, 117, 685
LweI GCATC 2 cut(s) 272, 567
MaeII ACGT 2 cut(s) 138, 534
MaeIII GTNAC 1 cut(s) 62
MalI GATC 4 cut(s) 276, 319, 352, 457
MboI GATC 4 cut(s) 274, 317, 350, 455
MboII GAAGA 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 23, 488
MluCI AATT 5 cut(s) 25, 90, 178, 421, 438
MmeI TCCRAC 1 cut(s) 510
MseI TTAA 2 cut(s) 195, 576
MspA1I CMGCKG 2 cut(s) 130, 676
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 473, 714
Mva1269I GAATGC 2 cut(s) 205, 500
MvaI CCWGG 2 cut(s) 473, 714
MwoI GCNNNNNNNGC 5 cut(s) 27, 130, 170, 369, 483
NdeII GATC 4 cut(s) 274, 317, 350, 455
NlaIII CATG 3 cut(s) 230, 324, 650
NlaIV GGNNCC 2 cut(s) 123, 165
NmuCI GTSAC 1 cut(s) 62
PagI TCATGA 1 cut(s) 226
PctI GAATGC 2 cut(s) 205, 500
PfeI GAWTC 2 cut(s) 98, 404
PkrI GCNGC 8 cut(s) 54, 57, 71, 126, 129, 132, 175, 675
Ple19I CGATCG 1 cut(s) 458
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 471, 712
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 2 cut(s) 471, 712
PspN4I GGNNCC 2 cut(s) 123, 165
PspPI GGNCC 1 cut(s) 601
PstI CTGCAG 1 cut(s) 568
PvuI CGATCG 1 cut(s) 458
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 195, 576
SatI GCNGC 8 cut(s) 53, 56, 70, 125, 128, 131, 174, 674
Sau3AI GATC 4 cut(s) 274, 317, 350, 455
Sau96I GGNCC 1 cut(s) 601
ScrFI CCNGG 2 cut(s) 473, 714
SduI GDGCHC 2 cut(s) 23, 488
SfaNI GCATC 2 cut(s) 272, 567
SfcI CTRYAG 2 cut(s) 564, 719
SinI GGWCC 1 cut(s) 601
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 25, 90, 178, 421, 438
SsiI CCGC 8 cut(s) 69, 80, 125, 128, 167, 171, 174, 676
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 471, 712
StyI CCWWGG 4 cut(s) 186, 553, 604, 633
TaaI ACNGT 3 cut(s) 62, 121, 619
TaiI ACGT 2 cut(s) 141, 537
TaqI TCGA 4 cut(s) 96, 292, 454, 659
TasI AATT 5 cut(s) 25, 90, 178, 421, 438
TatI WGTACW 1 cut(s) 619
TauI GCSGC 4 cut(s) 72, 127, 130, 176
TfiI GAWTC 2 cut(s) 98, 404
Tru1I TTAA 2 cut(s) 195, 576
Tru9I TTAA 2 cut(s) 195, 576
TseFI GTSAC 1 cut(s) 62
TseI GCWGC 4 cut(s) 52, 55, 130, 673
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 3 cut(s) 243, 276, 329
TspGWI ACGGA 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.