Rw7G007790

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0004996
Physical Location & Seq
Reverse (-)
7133171 .. 7134856
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007790.1

Sequence Viewer

Length: 735 bp
ATGAGCAATGACACGGAGAGCTCAAATTGCGAGCCTCCTGACCTTGAGAAGCAGCAGCACCGTGACGGCGGCGCTGAGGCGGTCACCTCAATTCCCGATTCTTCTGCCGTTGAGGATACGGTGCCGCCGCTGCTGAACGTCGTCGTTTCCGAACCACCACCGGCCCCGCCCGCCGCCGCCGCCACCGCCAATTCGTCCAAGGATGTTAAGGGACAGCATTCCAGAAACTCCAGCGCTCATGAACAATGCAGGGTGTGTCAGCAGGATAAAGATGAAGCTTTGATTGATCTCGGATGCCATTGTCGAGGTGAACTTGCGAAAGCGCACAGATCATGTATAGACACTTGGTTTCAATCCAAAGGATCAAACAAATGCGAGATTTGCCAAGAGGTAGCTGCGAATGTGTCATCTCCAGAATCCCAGCCAACTACAAATTATTGGGTTTGGAGAATTGAAACAACCTATCGATCGCAAAACCCAGACCAGGGCTGTATGAGCCCACTTTGGGTGGCATTCTCAATCCTCATTGGTGGTCTGCTGTTGGACGTGCTAATATCTGCCACCCTAGGCATCTCTGCAGTTCCCATTAACATTATCATCGGTGTTATTGTGGTCCTTGGGCTTGGAACTGTACTGAGACTTGCCTTGGCGTTTTGCCATGAGTGGGGTTCGAGGAGAGTTGTGCAGCGGGTGGACACACAGAGAGTGCCTCTTGGCTACCATCCTGGTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

244

Amino Acids

26.33

Weight (kDa)

5.33

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 83 - 128 6.4e-12 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AclWI GGATC 1 cut(s) 370
AfaI GTAC 1 cut(s) 633
AfeI AGCGCT 1 cut(s) 235
AfiI CCNNNNNNNGG 4 cut(s) 484, 485, 505, 664
AgsI TTSAA 2 cut(s) 353, 455
AjiI CACGTC 1 cut(s) 547
AjnI CCWGG 2 cut(s) 483, 724
AluBI AGCT 3 cut(s) 21, 278, 395
AluI AGCT 3 cut(s) 21, 278, 395
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 631
AlwI GGATC 1 cut(s) 370
Aor51HI AGCGCT 1 cut(s) 235
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 5 cut(s) 52, 55, 130, 395, 685
AspA2I CCTAGG 1 cut(s) 565
AspLEI GCGC 3 cut(s) 74, 236, 325
AspS9I GGNCC 2 cut(s) 163, 613
AsuHPI GGTGA 2 cut(s) 76, 320
AvaII GGWCC 1 cut(s) 613
AvrII CCTAGG 1 cut(s) 565
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 2 cut(s) 23, 500
Bbv12I GWGCWC 1 cut(s) 23
BbvCI CCTCAGC 1 cut(s) 75
BbvI GCAGC 5 cut(s) 64, 67, 117, 382, 697
BccI CCATC 1 cut(s) 729
BceAI ACGGC 2 cut(s) 82, 92
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 485, 726
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 631
BfaI CTAG 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 576
BfoI RGCGCY 2 cut(s) 75, 237
BfuI GTATCC 1 cut(s) 109
BlnI CCTAGG 1 cut(s) 565
Bme1390I CCNGG 2 cut(s) 485, 726
Bme18I GGWCC 1 cut(s) 613
BmgBI CACGTC 1 cut(s) 547
BmgT120I GGNCC 2 cut(s) 163, 613
BmiI GGNNCC 2 cut(s) 123, 165
BmrFI CCNGG 2 cut(s) 485, 726
BmsI GCATC 2 cut(s) 284, 579
BplI GAGNNNNNCTC 2 cut(s) 694, 726
BpmI CTGGAG 2 cut(s) 214, 396
Bpu10I CCTNAGC 1 cut(s) 75
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 466
BsaJI CCNNGG 5 cut(s) 198, 484, 565, 616, 645
Bsc4I CCNNNNNNNGG 4 cut(s) 484, 485, 505, 664
Bse118I RCCGGY 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 13
BseBI CCWGG 2 cut(s) 485, 726
BseCI ATCGAT 1 cut(s) 466
BseDI CCNNGG 5 cut(s) 198, 484, 565, 616, 645
BseGI GGATG 3 cut(s) 208, 299, 721
BseLI CCNNNNNNNGG 4 cut(s) 484, 485, 505, 664
BseMI GCAATG 1 cut(s) 13
BseMII CTCAG 2 cut(s) 66, 626
BseRI GAGGAG 1 cut(s) 688
BseXI GCAGC 5 cut(s) 64, 67, 117, 382, 697
BseYI CCCAGC 1 cut(s) 420
BsgI GTGCAG 1 cut(s) 704
Bsh1285I CGRYCG 1 cut(s) 470
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 1 cut(s) 121
BshVI ATCGAT 1 cut(s) 466
BsiEI CGRYCG 1 cut(s) 470
BsiHKAI GWGCWC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 161
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 4 cut(s) 484, 485, 505, 664
BsmAI GTCTC 1 cut(s) 631
BsmFI GGGAC 1 cut(s) 225
BsmI GAATGC 2 cut(s) 217, 512
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 23, 500
Bsp143I GATC 4 cut(s) 286, 329, 362, 467
BspANI GGCC 1 cut(s) 164
BspCNI CTCAG 2 cut(s) 67, 627
BspDI ATCGAT 1 cut(s) 466
BspHI TCATGA 1 cut(s) 238
BspLI GGNNCC 2 cut(s) 123, 165
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 1 cut(s) 370
BspT107I GGYRCC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 160
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 5 cut(s) 198, 484, 565, 616, 645
BssMI GATC 4 cut(s) 286, 329, 362, 467
BssT1I CCWWGG 4 cut(s) 198, 565, 616, 645
Bst2UI CCWGG 2 cut(s) 485, 726
Bst4CI ACNGT 3 cut(s) 62, 121, 631
BstC8I GCNNGC 2 cut(s) 32, 171
BstDEI CTNAG 2 cut(s) 75, 635
BstEII GGTNACC 1 cut(s) 82
BstF5I GGATG 3 cut(s) 208, 299, 721
BstH2I RGCGCY 2 cut(s) 75, 237
BstHHI GCGC 3 cut(s) 74, 236, 325
BstKTI GATC 4 cut(s) 289, 332, 365, 470
BstMAI GTCTC 1 cut(s) 631
BstMBI GATC 4 cut(s) 286, 329, 362, 467
BstMCI CGRYCG 1 cut(s) 470
BstMWI GCNNNNNNNGC 7 cut(s) 27, 130, 170, 179, 185, 381, 495
BstNI CCWGG 2 cut(s) 485, 726
BstPI GGTNACC 1 cut(s) 82
BstSCI CCNGG 2 cut(s) 483, 724
BstSFI CTRYAG 1 cut(s) 576
BstV1I GCAGC 5 cut(s) 64, 67, 117, 382, 697
Bsu15I ATCGAT 1 cut(s) 466
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 1 cut(s) 164
BsuTUI ATCGAT 1 cut(s) 466
BtrI CACGTC 1 cut(s) 547
BtsCI GGATG 3 cut(s) 208, 299, 721
Cac8I GCNNGC 2 cut(s) 32, 171
CciI TCATGA 1 cut(s) 238
CfoI GCGC 3 cut(s) 74, 236, 325
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 163, 613
ClaI ATCGAT 1 cut(s) 466
Csp6I GTAC 1 cut(s) 632
CviAII CATG 3 cut(s) 239, 333, 659
CviQI GTAC 1 cut(s) 632
DdeI CTNAG 2 cut(s) 75, 635
DpnI GATC 4 cut(s) 288, 331, 364, 469
DpnII GATC 4 cut(s) 286, 329, 362, 467
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 4 cut(s) 198, 565, 616, 645
Eco24I GRGCYC 2 cut(s) 23, 500
Eco47I GGWCC 1 cut(s) 613
Eco47III AGCGCT 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 21
Eco91I GGTNACC 1 cut(s) 82
EcoICRI GAGCTC 1 cut(s) 21
EcoO65I GGTNACC 1 cut(s) 82
EcoRII CCWGG 2 cut(s) 483, 724
EcoT14I CCWWGG 4 cut(s) 198, 565, 616, 645
EcoT38I GRGCYC 2 cut(s) 23, 500
ErhI CCWWGG 4 cut(s) 198, 565, 616, 645
FaeI CATG 3 cut(s) 242, 336, 662
FaiI YATR 5 cut(s) 240, 334, 338, 494, 660
FaqI GGGAC 1 cut(s) 225
FatI CATG 3 cut(s) 238, 332, 658
FauI CCCGC 3 cut(s) 174, 178, 681
FokI GGATG 3 cut(s) 215, 306, 708
FriOI GRGCYC 2 cut(s) 23, 500
FspBI CTAG 1 cut(s) 566
GlaI GCGC 3 cut(s) 73, 235, 324
GsaI CCCAGC 1 cut(s) 424
GsuI CTGGAG 2 cut(s) 214, 396
HaeII RGCGCY 2 cut(s) 75, 237
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 161
HhaI GCGC 3 cut(s) 74, 236, 325
Hin1II CATG 3 cut(s) 242, 336, 662
Hin6I GCGC 3 cut(s) 72, 234, 323
HinP1I GCGC 3 cut(s) 72, 234, 323
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 2 cut(s) 98, 416
HpaII CCGG 1 cut(s) 161
HphI GGTGA 2 cut(s) 76, 320
Hpy166II GTNNAC 2 cut(s) 311, 694
Hpy188I TCNGA 2 cut(s) 151, 293
Hpy188III TCNNGA 5 cut(s) 38, 95, 222, 239, 413
Hpy8I GTNNAC 2 cut(s) 311, 694
Hpy99I CGWCG 2 cut(s) 143, 146
HpyCH4III ACNGT 3 cut(s) 62, 121, 631
HpyCH4IV ACGT 2 cut(s) 138, 546
HpyCH4V TGCA 3 cut(s) 249, 578, 685
HpyF10VI GCNNNNNNNGC 7 cut(s) 27, 130, 170, 179, 185, 381, 495
HpyF3I CTNAG 2 cut(s) 75, 635
HpySE526I ACGT 2 cut(s) 138, 546
Hsp92II CATG 3 cut(s) 242, 336, 662
HspAI GCGC 3 cut(s) 72, 234, 323
Kzo9I GATC 4 cut(s) 286, 329, 362, 467
Lsp1109I GCAGC 5 cut(s) 64, 67, 117, 382, 697
LweI GCATC 2 cut(s) 284, 579
MaeI CTAG 1 cut(s) 566
MaeII ACGT 2 cut(s) 138, 546
MaeIII GTNAC 2 cut(s) 62, 82
MalI GATC 4 cut(s) 288, 331, 364, 469
MboI GATC 4 cut(s) 286, 329, 362, 467
MboII GAAGA 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 23, 500
MluCI AATT 5 cut(s) 25, 90, 190, 433, 450
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 9 cut(s) 45, 70, 97, 106, 299, 382, 533, 666, 720
MseI TTAA 2 cut(s) 207, 588
MspA1I CMGCKG 2 cut(s) 130, 688
MspI CCGG 1 cut(s) 161
MspR9I CCNGG 2 cut(s) 485, 726
Mva1269I GAATGC 2 cut(s) 217, 512
MvaI CCWGG 2 cut(s) 485, 726
MwoI GCNNNNNNNGC 7 cut(s) 27, 130, 170, 179, 185, 381, 495
NdeII GATC 4 cut(s) 286, 329, 362, 467
NlaIII CATG 3 cut(s) 242, 336, 662
NlaIV GGNNCC 2 cut(s) 123, 165
NmuCI GTSAC 2 cut(s) 62, 82
PagI TCATGA 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PctI GAATGC 2 cut(s) 217, 512
PfeI GAWTC 2 cut(s) 98, 416
Ple19I CGATCG 1 cut(s) 470
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 483, 724
PspEI GGTNACC 1 cut(s) 82
PspFI CCCAGC 1 cut(s) 420
PspGI CCWGG 2 cut(s) 483, 724
PspN4I GGNNCC 2 cut(s) 123, 165
PspPI GGNCC 2 cut(s) 163, 613
PstI CTGCAG 1 cut(s) 580
PvuI CGATCG 1 cut(s) 470
RsaI GTAC 1 cut(s) 633
RsaNI GTAC 1 cut(s) 632
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 207, 588
Sau3AI GATC 4 cut(s) 286, 329, 362, 467
Sau96I GGNCC 2 cut(s) 163, 613
ScrFI CCNGG 2 cut(s) 485, 726
SduI GDGCHC 2 cut(s) 23, 500
SfaNI GCATC 2 cut(s) 284, 579
SfcI CTRYAG 1 cut(s) 576
SinI GGWCC 1 cut(s) 613
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 25, 90, 190, 433, 450
SspMI CTAG 1 cut(s) 566
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 483, 724
StyI CCWWGG 4 cut(s) 198, 565, 616, 645
TaaI ACNGT 3 cut(s) 62, 121, 631
TaiI ACGT 2 cut(s) 141, 549
TaqI TCGA 3 cut(s) 304, 466, 671
TasI AATT 5 cut(s) 25, 90, 190, 433, 450
TatI WGTACW 1 cut(s) 631
TauI GCSGC 6 cut(s) 72, 127, 130, 176, 179, 182
TfiI GAWTC 2 cut(s) 98, 416
Tru1I TTAA 2 cut(s) 207, 588
Tru9I TTAA 2 cut(s) 207, 588
TseFI GTSAC 2 cut(s) 62, 82
TseI GCWGC 5 cut(s) 52, 55, 130, 395, 685
Tsp45I GTSAC 2 cut(s) 62, 82
TspDTI ATGAA 2 cut(s) 255, 288
TspGWI ACGGA 1 cut(s) 29
VpaK11BI GGWCC 1 cut(s) 613
XmaJI CCTAGG 1 cut(s) 565
XspI CTAG 1 cut(s) 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.