MD06G1124900.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0004996
Physical Location & Seq
Reverse (-)
26701497 .. 26703758
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1124900.v1.1.491

Sequence Viewer

Length: 753 bp
ATGGGGGGTGAGAAAGAGAGTGAAAATGGAGAGTTACCTGACCTGGAGAAGCAGCAGCTAGGCGGTCCTGAGACTAGCTCACTTCCCAGTTTGGCAACTGTGGATACGGTGCTGCCGCTACAACAGGTCGTCGTTTCGGGTGGGGCGTCATCTGAAGTTCATGAACCGCCGACAGTCACCGCCACGGTCAAATTTTCAGAGGAGGTTCCTTCTCCCAAAAAGGACCACCTGTCGAGGACTTCGAGCTCTCATGAACAATGCAGAGTGTGTCAGCAGGAGAAAGATGAAGTTTTGATTGATCTCGGATGCCAGTGTCGAGGCGGGCTTGCGAAATCCCACAGATCATGTATAGACACTTGGTTTCGCTCCAGGGGATCGAACAAATGCGAGATATGCCAAGGAGTAGCAGCAAATGTTCCACCTCCAGAGGCTCAGCCAACTGCAAGCTATTGGGTTTGGAGAGTTGGCTCATCCTACCGATCACAAAATCGAGACAGGGGTTGTGTAAGCCCACTTTATGTGGCGTTTTCAATCCTTATAGGTGGTCTACTGTTGGACGTGCTAATTTCTGTCACCCTTGGTGTCTCCGCACTGCCCATCAACATCATTATAGGTATTATCGTGGTTCTTGGACTCGGCACTGCACTGAGACTCGCCCTGGAATTCTTCCACGAGTGGAGTGTGAGGAGAGTCGTGCAGAGAGTCGACGCAAGTCACACGAATGTGCCTCTTGGTTACCATCCTACTTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

251

Amino Acids

27.08

Weight (kDa)

5.98

Isoelectric Point (pI)

45.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 87 - 132 4.2e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 547, 705
AciI CCGC 6 cut(s) 63, 116, 167, 180, 321, 588
AclWI GGATC 1 cut(s) 382
AcsI RAATTY 2 cut(s) 191, 662
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 146
AgsI TTSAA 1 cut(s) 531
AhdI GACNNNNNGTC 1 cut(s) 229
AjiI CACGTC 1 cut(s) 559
AjnI CCWGG 3 cut(s) 42, 368, 657
AleI CACNNNNGTG 1 cut(s) 722
AluBI AGCT 4 cut(s) 58, 78, 246, 447
AluI AGCT 4 cut(s) 58, 78, 246, 447
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 4 cut(s) 65, 486, 589, 643
AlwI GGATC 1 cut(s) 382
ApeKI GCWGC 4 cut(s) 52, 55, 112, 407
ApoI RAATTY 2 cut(s) 191, 662
AspS9I GGNCC 2 cut(s) 65, 223
AsuHPI GGTGA 3 cut(s) 20, 169, 565
AvaII GGWCC 2 cut(s) 65, 223
BanII GRGCYC 1 cut(s) 248
BauI CACGAG 1 cut(s) 671
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 4 cut(s) 64, 67, 99, 419
BccI CCATC 2 cut(s) 605, 747
BciT130I CCWGG 3 cut(s) 44, 370, 659
BciVI GTATCC 1 cut(s) 97
BcoDI GTCTC 4 cut(s) 65, 486, 589, 643
BfaI CTAG 2 cut(s) 59, 75
BfuI GTATCC 1 cut(s) 97
BisI GCNGC 5 cut(s) 53, 56, 113, 116, 408
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 5 cut(s) 54, 57, 114, 117, 409
Bme1390I CCNGG 3 cut(s) 44, 370, 659
Bme18I GGWCC 2 cut(s) 65, 223
BmeRI GACNNNNNGTC 1 cut(s) 229
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 2 cut(s) 65, 223
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 3 cut(s) 44, 370, 659
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 81
BoxI GACNNNNGTC 1 cut(s) 711
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpmI CTGGAG 3 cut(s) 65, 352, 408
Bpu1102I GCTNAGC 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 5 cut(s) 183, 369, 397, 577, 657
Bse1I ACTGG 2 cut(s) 87, 310
BseBI CCWGG 3 cut(s) 44, 370, 659
BseDI CCNNGG 5 cut(s) 183, 369, 397, 577, 657
BseGI GGATG 3 cut(s) 311, 470, 739
BseMII CTCAG 3 cut(s) 60, 446, 638
BseNI ACTGG 2 cut(s) 87, 310
BseRI GAGGAG 2 cut(s) 215, 700
BseXI GCAGC 4 cut(s) 64, 67, 99, 419
BsgI GTGCAG 2 cut(s) 627, 716
BsiHKAI GWGCWC 1 cut(s) 248
BsmAI GTCTC 4 cut(s) 65, 486, 589, 643
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 4 cut(s) 298, 341, 374, 479
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 6 cut(s) 63, 116, 167, 180, 321, 588
BspCNI CTCAG 3 cut(s) 61, 445, 639
BspHI TCATGA 2 cut(s) 160, 250
BspLI GGNNCC 1 cut(s) 207
BspPI GGATC 1 cut(s) 382
BsrI ACTGG 2 cut(s) 87, 310
BssECI CCNNGG 5 cut(s) 183, 369, 397, 577, 657
BssMI GATC 4 cut(s) 298, 341, 374, 479
BssNI GRCGYC 1 cut(s) 146
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 2 cut(s) 397, 577
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 3 cut(s) 44, 370, 659
Bst4CI ACNGT 5 cut(s) 100, 109, 175, 187, 552
BstACI GRCGYC 1 cut(s) 146
BstC8I GCNNGC 3 cut(s) 323, 327, 445
BstDEI CTNAG 3 cut(s) 69, 432, 647
BstDSI CCRYGG 1 cut(s) 183
BstEII GGTNACC 1 cut(s) 734
BstF5I GGATG 3 cut(s) 311, 470, 739
BstKTI GATC 4 cut(s) 301, 344, 377, 482
BstMAI GTCTC 4 cut(s) 65, 486, 589, 643
BstMBI GATC 4 cut(s) 298, 341, 374, 479
BstMWI GCNNNNNNNGC 1 cut(s) 393
BstNI CCWGG 3 cut(s) 44, 370, 659
BstPAI GACNNNNGTC 1 cut(s) 711
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 3 cut(s) 42, 368, 657
BstV1I GCAGC 4 cut(s) 64, 67, 99, 419
BsuI GTATCC 1 cut(s) 97
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 3 cut(s) 311, 470, 739
BtsI GCAGTG 2 cut(s) 590, 639
BtsIMutI CAGTG 4 cut(s) 317, 590, 639, 644
Cac8I GCNNGC 3 cut(s) 323, 327, 445
CciI TCATGA 2 cut(s) 160, 250
Cfr13I GGNCC 2 cut(s) 65, 223
CseI GACGC 2 cut(s) 135, 716
CviAII CATG 3 cut(s) 161, 251, 345
CviJI RGCY 9 cut(s) 58, 78, 246, 325, 431, 436, 447, 468, 510
CviKI_1 RGCY 9 cut(s) 58, 78, 246, 325, 431, 436, 447, 468, 510
DdeI CTNAG 3 cut(s) 69, 432, 647
DpnI GATC 4 cut(s) 300, 343, 376, 481
DpnII GATC 4 cut(s) 298, 341, 374, 479
DriI GACNNNNNGTC 1 cut(s) 229
Eam1105I GACNNNNNGTC 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 2 cut(s) 397, 577
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 2 cut(s) 65, 223
Eco53kI GAGCTC 1 cut(s) 246
Eco57I CTGAAG 1 cut(s) 174
Eco91I GGTNACC 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 246
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 1 cut(s) 662
EcoRII CCWGG 3 cut(s) 42, 368, 657
EcoT14I CCWWGG 2 cut(s) 397, 577
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 2 cut(s) 397, 577
FaeI CATG 3 cut(s) 164, 254, 348
FaiI YATR 8 cut(s) 162, 252, 346, 350, 394, 519, 539, 611
FatI CATG 3 cut(s) 160, 250, 344
FauI CCCGC 1 cut(s) 314
FblI GTMKAC 2 cut(s) 547, 705
Fnu4HI GCNGC 5 cut(s) 53, 56, 113, 116, 408
FokI GGATG 3 cut(s) 318, 457, 726
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 5 cut(s) 53, 56, 113, 116, 408
FspBI CTAG 2 cut(s) 59, 75
GluI GCNGC 5 cut(s) 53, 56, 113, 116, 408
GsuI CTGGAG 3 cut(s) 65, 352, 408
HgaI GACGC 2 cut(s) 135, 716
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 3 cut(s) 164, 254, 348
HincII GTYRAC 1 cut(s) 706
HindII GTYRAC 1 cut(s) 706
HinfI GANTC 4 cut(s) 633, 651, 690, 702
HphI GGTGA 3 cut(s) 20, 169, 565
Hpy166II GTNNAC 2 cut(s) 548, 706
Hpy188I TCNGA 3 cut(s) 154, 199, 305
Hpy188III TCNNGA 5 cut(s) 68, 161, 251, 425, 491
Hpy8I GTNNAC 2 cut(s) 548, 706
Hpy99I CGWCG 2 cut(s) 134, 710
HpyAV CCTTC 1 cut(s) 219
HpyCH4III ACNGT 5 cut(s) 100, 109, 175, 187, 552
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 4 cut(s) 261, 443, 644, 697
HpyF10VI GCNNNNNNNGC 1 cut(s) 393
HpyF3I CTNAG 3 cut(s) 69, 432, 647
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 3 cut(s) 164, 254, 348
Kzo9I GATC 4 cut(s) 298, 341, 374, 479
LmnI GCTCC 1 cut(s) 371
Lsp1109I GCAGC 4 cut(s) 64, 67, 99, 419
LweI GCATC 1 cut(s) 296
MaeI CTAG 2 cut(s) 59, 75
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 5 cut(s) 33, 175, 571, 713, 734
MalI GATC 4 cut(s) 300, 343, 376, 481
MboI GATC 4 cut(s) 298, 341, 374, 479
MboII GAAGA 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 3 cut(s) 191, 564, 662
MlyI GAGTC 4 cut(s) 627, 645, 699, 711
MmeI TCCRAC 1 cut(s) 534
MnlI CCTC 8 cut(s) 193, 196, 228, 311, 421, 432, 678, 738
MslI CAYNNNNRTG 2 cut(s) 720, 722
MspR9I CCNGG 3 cut(s) 44, 370, 659
MvaI CCWGG 3 cut(s) 44, 370, 659
MwoI GCNNNNNNNGC 1 cut(s) 393
NdeII GATC 4 cut(s) 298, 341, 374, 479
NlaIII CATG 3 cut(s) 164, 254, 348
NlaIV GGNNCC 1 cut(s) 207
NmeAIII GCCGAG 1 cut(s) 615
NmuCI GTSAC 3 cut(s) 175, 571, 713
OliI CACNNNNGTG 1 cut(s) 722
PagI TCATGA 2 cut(s) 160, 250
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 239
PkrI GCNGC 5 cut(s) 54, 57, 114, 117, 409
PleI GAGTC 4 cut(s) 627, 645, 698, 710
PpsI GAGTC 4 cut(s) 627, 645, 698, 710
PshAI GACNNNNGTC 1 cut(s) 711
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 3 cut(s) 42, 368, 657
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 3 cut(s) 42, 368, 657
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 2 cut(s) 65, 223
RseI CAYNNNNRTG 2 cut(s) 720, 722
SacI GAGCTC 1 cut(s) 248
SalI GTCGAC 1 cut(s) 704
SatI GCNGC 5 cut(s) 53, 56, 113, 116, 408
Sau3AI GATC 4 cut(s) 298, 341, 374, 479
Sau96I GGNCC 2 cut(s) 65, 223
SchI GAGTC 4 cut(s) 627, 645, 699, 711
ScrFI CCNGG 3 cut(s) 44, 370, 659
SduI GDGCHC 1 cut(s) 248
SfaNI GCATC 1 cut(s) 296
SinI GGWCC 2 cut(s) 65, 223
SmiMI CAYNNNNRTG 2 cut(s) 720, 722
Sse9I AATT 3 cut(s) 191, 564, 662
SsiI CCGC 6 cut(s) 63, 116, 167, 180, 321, 588
SspMI CTAG 2 cut(s) 59, 75
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 3 cut(s) 42, 368, 657
StyI CCWWGG 2 cut(s) 397, 577
TaaI ACNGT 5 cut(s) 100, 109, 175, 187, 552
TaiI ACGT 1 cut(s) 561
TaqI TCGA 6 cut(s) 233, 242, 316, 377, 490, 705
TasI AATT 3 cut(s) 191, 564, 662
TauI GCSGC 1 cut(s) 118
TscAI CASTG 4 cut(s) 317, 597, 646, 651
TseFI GTSAC 3 cut(s) 175, 571, 713
TseI GCWGC 4 cut(s) 52, 55, 112, 407
Tsp45I GTSAC 3 cut(s) 175, 571, 713
TspDTI ATGAA 4 cut(s) 149, 177, 267, 300
TspRI CASTG 4 cut(s) 317, 597, 646, 651
VpaK11BI GGWCC 2 cut(s) 65, 223
XapI RAATTY 2 cut(s) 191, 662
XmiI GTMKAC 2 cut(s) 547, 705
XspI CTAG 2 cut(s) 59, 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.