FvH4_5g10340

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004996
Physical Location & Seq
Forward (+)
5900329 .. 5902527
2199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g10340.t1

Sequence Viewer

Length: 714 bp
ATGAGCAGAAACAACGAGACTTCAAATTCCGACGAGCCTCCTGACCTCGAGAAGCAGCAGCAGCAGCCTGACGGCGCCGTTGAGGCGGTTACCTCAATTCCCGATTCTTCTGCCGCACCGCCGCTGCTGAATGTCGTCGTTTCGCAACCACCGCCTCCCGTCGCCGCCGCCAATCTGTCAAAGGAGGCTAAGGGACAGCACTCGAGAAGCTCCAGTGCTCATGAACAATGCAGAGTGTGTCAGCAGGATAAAGATGAAGCTTTGATTGATCTGGGATGCCATTGTCGAGGTGGACTTGCGAAAGCGCATAGATCATGTATAGACACTTGGTTTCGGAGCAAAGGATCCAACAAATGCGAGATTTGCCAAGGGGTAGCTAGCAATGTGTCGTCTCCAGAATCCCAGCCAACTACAAATTATTGGGTTTGGAGAATTGAAACAACCTATCGATCGCAAAATCAAGACCGGGGCTGTAACCCCCTTTGGGTGGCATTCTCAATCCTCATAGGTGGTCTGATGTTGGATGTGCTAATATCTACCACCCTTGGCATCTCTGCTGTCCCCGTTAACATTATCATTGGTGTTATTGTGGTCCTTGGGCTTGGAACTATACTGAGACTTGCCTTGGCGTTTTGCCATGAATGGAGTACGAGAAGTGTTGTACAGCGAGTGGAAACACAGAGAGTGCCTCTTGGCTACCATCCTGATCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

238

Amino Acids

25.75

Weight (kDa)

6.06

Isoelectric Point (pI)

53.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 77 - 122 1e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 74
AciI CCGC 7 cut(s) 86, 114, 119, 122, 152, 165, 168
AclWI GGATC 2 cut(s) 339, 352
AcsI RAATTY 1 cut(s) 25
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 649, 663
AfiI CCNNNNNNNGG 2 cut(s) 484, 487
AgsI TTSAA 2 cut(s) 24, 437
AluBI AGCT 3 cut(s) 210, 260, 377
AluI AGCT 3 cut(s) 210, 260, 377
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 3 cut(s) 11, 396, 610
AlwI GGATC 2 cut(s) 339, 352
Ama87I CYCGRG 2 cut(s) 47, 202
ApeKI GCWGC 5 cut(s) 55, 58, 61, 64, 124
ApoI RAATTY 1 cut(s) 25
AspLEI GCGC 2 cut(s) 77, 307
AspS9I GGNCC 1 cut(s) 592
AsuC2I CCSGG 1 cut(s) 467
AsuNHI GCTAGC 1 cut(s) 377
AvaI CYCGRG 2 cut(s) 47, 202
AvaII GGWCC 1 cut(s) 592
BamHI GGATCC 1 cut(s) 344
BanI GGYRCC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 5 cut(s) 67, 70, 73, 76, 111
BccI CCATC 1 cut(s) 708
BceAI ACGGC 2 cut(s) 62, 88
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 3 cut(s) 11, 396, 610
BfaI CTAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 78
BglI GCCNNNNNGGC 1 cut(s) 83
BisI GCNGC 9 cut(s) 56, 59, 62, 65, 114, 122, 125, 165, 168
BlsI GCNGC 9 cut(s) 57, 60, 63, 66, 115, 123, 126, 166, 169
Bme1390I CCNGG 1 cut(s) 467
Bme18I GGWCC 1 cut(s) 592
BmeT110I CYCGRG 2 cut(s) 47, 202
BmgT120I GGNCC 1 cut(s) 592
BmiI GGNNCC 2 cut(s) 76, 346
BmrFI CCNGG 1 cut(s) 467
BmsI GCATC 2 cut(s) 266, 558
BmtI GCTAGC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 673, 705
BpmI CTGGAG 2 cut(s) 196, 378
Bpu10I CCTNAGC 1 cut(s) 189
BpuMI CCSGG 1 cut(s) 467
Bsa29I ATCGAT 1 cut(s) 448
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 5 cut(s) 367, 466, 544, 595, 624
Bsc4I CCNNNNNNNGG 2 cut(s) 484, 487
Bse1I ACTGG 1 cut(s) 213
Bse3DI GCAATG 1 cut(s) 388
BseCI ATCGAT 1 cut(s) 448
BseDI CCNNGG 5 cut(s) 367, 466, 544, 595, 624
BseGI GGATG 3 cut(s) 281, 529, 700
BseLI CCNNNNNNNGG 2 cut(s) 484, 487
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 1 cut(s) 605
BseNI ACTGG 1 cut(s) 213
BseXI GCAGC 5 cut(s) 67, 70, 73, 76, 111
BseYI CCCAGC 1 cut(s) 402
Bsh1285I CGRYCG 1 cut(s) 452
BshNI GGYRCC 1 cut(s) 74
BshVI ATCGAT 1 cut(s) 448
BsiEI CGRYCG 1 cut(s) 452
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 2 cut(s) 47, 202
BsiSI CCGG 1 cut(s) 466
BslFI GGGAC 2 cut(s) 207, 545
BslI CCNNNNNNNGG 2 cut(s) 484, 487
BsmAI GTCTC 3 cut(s) 11, 396, 610
BsmBI CGTCTC 1 cut(s) 396
BsmFI GGGAC 2 cut(s) 207, 545
BsmI GAATGC 1 cut(s) 491
BsoBI CYCGRG 2 cut(s) 47, 202
Bsp1286I GDGCHC 1 cut(s) 220
Bsp1407I TGTACA 1 cut(s) 661
Bsp143I GATC 5 cut(s) 268, 311, 344, 449, 706
BspACI CCGC 7 cut(s) 86, 114, 119, 122, 152, 165, 168
BspCNI CTCAG 1 cut(s) 606
BspDI ATCGAT 1 cut(s) 448
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 76, 346
BspOI GCTAGC 1 cut(s) 381
BspPI GGATC 2 cut(s) 339, 352
BspT107I GGYRCC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 388
BsrGI TGTACA 1 cut(s) 661
BsrI ACTGG 1 cut(s) 213
BssECI CCNNGG 5 cut(s) 367, 466, 544, 595, 624
BssMI GATC 5 cut(s) 268, 311, 344, 449, 706
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 4 cut(s) 367, 544, 595, 624
BstACI GRCGYC 1 cut(s) 75
BstAUI TGTACA 1 cut(s) 661
BstC8I GCNNGC 1 cut(s) 379
BstDEI CTNAG 2 cut(s) 189, 614
BstEII GGTNACC 1 cut(s) 88
BstF5I GGATG 3 cut(s) 281, 529, 700
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 2 cut(s) 77, 307
BstKTI GATC 5 cut(s) 271, 314, 347, 452, 709
BstMAI GTCTC 3 cut(s) 11, 396, 610
BstMBI GATC 5 cut(s) 268, 311, 344, 449, 706
BstMCI CGRYCG 1 cut(s) 452
BstMWI GCNNNNNNNGC 5 cut(s) 61, 64, 83, 151, 363
BstPI GGTNACC 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 465
BstV1I GCAGC 5 cut(s) 67, 70, 73, 76, 111
BstX2I RGATCY 1 cut(s) 344
BstYI RGATCY 1 cut(s) 344
Bsu15I ATCGAT 1 cut(s) 448
BsuTUI ATCGAT 1 cut(s) 448
BtsCI GGATG 3 cut(s) 281, 529, 700
BtsIMutI CAGTG 1 cut(s) 220
Cac8I GCNNGC 1 cut(s) 379
CciI TCATGA 1 cut(s) 220
CfoI GCGC 2 cut(s) 77, 307
Cfr13I GGNCC 1 cut(s) 592
ClaI ATCGAT 1 cut(s) 448
Csp6I GTAC 2 cut(s) 648, 662
CviAII CATG 3 cut(s) 221, 315, 638
CviQI GTAC 2 cut(s) 648, 662
DdeI CTNAG 2 cut(s) 189, 614
DinI GGCGCC 1 cut(s) 76
DpnI GATC 5 cut(s) 270, 313, 346, 451, 708
DpnII GATC 5 cut(s) 268, 311, 344, 449, 706
Eco130I CCWWGG 4 cut(s) 367, 544, 595, 624
Eco47I GGWCC 1 cut(s) 592
Eco88I CYCGRG 2 cut(s) 47, 202
Eco91I GGTNACC 1 cut(s) 88
EcoO65I GGTNACC 1 cut(s) 88
EcoT14I CCWWGG 4 cut(s) 367, 544, 595, 624
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 4 cut(s) 367, 544, 595, 624
Esp3I CGTCTC 1 cut(s) 396
FaeI CATG 3 cut(s) 224, 318, 641
FaiI YATR 7 cut(s) 222, 309, 316, 320, 506, 611, 639
FaqI GGGAC 2 cut(s) 207, 545
FatI CATG 3 cut(s) 220, 314, 637
Fnu4HI GCNGC 9 cut(s) 56, 59, 62, 65, 114, 122, 125, 165, 168
FokI GGATG 3 cut(s) 288, 536, 687
Fsp4HI GCNGC 9 cut(s) 56, 59, 62, 65, 114, 122, 125, 165, 168
FspBI CTAG 1 cut(s) 378
GlaI GCGC 2 cut(s) 76, 306
GluI GCNGC 9 cut(s) 56, 59, 62, 65, 114, 122, 125, 165, 168
GsaI CCCAGC 1 cut(s) 406
GsuI CTGGAG 2 cut(s) 196, 378
HaeII RGCGCY 1 cut(s) 78
HapII CCGG 1 cut(s) 466
HhaI GCGC 2 cut(s) 77, 307
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 3 cut(s) 224, 318, 641
Hin6I GCGC 2 cut(s) 75, 305
HinP1I GCGC 2 cut(s) 75, 305
HincII GTYRAC 1 cut(s) 568
HindII GTYRAC 1 cut(s) 568
HindIII AAGCTT 1 cut(s) 258
HinfI GANTC 2 cut(s) 104, 398
HpaI GTTAAC 1 cut(s) 568
HpaII CCGG 1 cut(s) 466
Hpy166II GTNNAC 2 cut(s) 293, 568
Hpy188I TCNGA 3 cut(s) 31, 336, 516
Hpy188III TCNNGA 8 cut(s) 41, 49, 101, 204, 221, 395, 461, 704
Hpy8I GTNNAC 2 cut(s) 293, 568
Hpy99I CGWCG 3 cut(s) 35, 140, 164
HpyCH4V TGCA 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 5 cut(s) 61, 64, 83, 151, 363
HpyF3I CTNAG 2 cut(s) 189, 614
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 3 cut(s) 224, 318, 641
HspAI GCGC 2 cut(s) 75, 305
KasI GGCGCC 1 cut(s) 74
KspAI GTTAAC 1 cut(s) 568
Kzo9I GATC 5 cut(s) 268, 311, 344, 449, 706
LmnI GCTCC 2 cut(s) 215, 336
LpnPI CCDG 8 cut(s) 54, 81, 226, 230, 257, 408, 416, 479
Lsp1109I GCAGC 5 cut(s) 67, 70, 73, 76, 111
LweI GCATC 2 cut(s) 266, 558
MaeI CTAG 1 cut(s) 378
MaeIII GTNAC 2 cut(s) 88, 473
MalI GATC 5 cut(s) 270, 313, 346, 451, 708
MboI GATC 5 cut(s) 268, 311, 344, 449, 706
MboII GAAGA 1 cut(s) 99
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 4 cut(s) 25, 96, 415, 432
Mly113I GGCGCC 1 cut(s) 75
MmeI TCCRAC 3 cut(s) 54, 372, 501
MnlI CCTC 9 cut(s) 48, 56, 76, 103, 165, 178, 281, 512, 699
MseI TTAA 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 124
MspI CCGG 1 cut(s) 466
MspR9I CCNGG 1 cut(s) 467
Mva1269I GAATGC 1 cut(s) 491
MwoI GCNNNNNNNGC 5 cut(s) 61, 64, 83, 151, 363
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 1 cut(s) 467
NdeII GATC 5 cut(s) 268, 311, 344, 449, 706
NheI GCTAGC 1 cut(s) 377
NlaIII CATG 3 cut(s) 224, 318, 641
NlaIV GGNNCC 2 cut(s) 76, 346
PaeR7I CTCGAG 2 cut(s) 47, 202
PagI TCATGA 1 cut(s) 220
PctI GAATGC 1 cut(s) 491
PfeI GAWTC 2 cut(s) 104, 398
PkrI GCNGC 9 cut(s) 57, 60, 63, 66, 115, 123, 126, 166, 169
Ple19I CGATCG 1 cut(s) 452
PluTI GGCGCC 1 cut(s) 78
PspEI GGTNACC 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 402
PspN4I GGNNCC 2 cut(s) 76, 346
PspPI GGNCC 1 cut(s) 592
PsuI RGATCY 1 cut(s) 344
PvuI CGATCG 1 cut(s) 452
RsaI GTAC 2 cut(s) 649, 663
RsaNI GTAC 2 cut(s) 648, 662
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 9 cut(s) 56, 59, 62, 65, 114, 122, 125, 165, 168
Sau3AI GATC 5 cut(s) 268, 311, 344, 449, 706
Sau96I GGNCC 1 cut(s) 592
ScrFI CCNGG 1 cut(s) 467
SduI GDGCHC 1 cut(s) 220
SetI ASST 8 cut(s) 48, 95, 212, 262, 292, 379, 446, 511
SfaNI GCATC 2 cut(s) 266, 558
SfoI GGCGCC 1 cut(s) 76
Sfr274I CTCGAG 2 cut(s) 47, 202
SinI GGWCC 1 cut(s) 592
SlaI CTCGAG 2 cut(s) 47, 202
SmlI CTYRAG 2 cut(s) 47, 202
SmoI CTYRAG 2 cut(s) 47, 202
Sse9I AATT 4 cut(s) 25, 96, 415, 432
SsiI CCGC 7 cut(s) 86, 114, 119, 122, 152, 165, 168
SspDI GGCGCC 1 cut(s) 74
SspMI CTAG 1 cut(s) 378
StyD4I CCNGG 1 cut(s) 465
StyI CCWWGG 4 cut(s) 367, 544, 595, 624
TaqI TCGA 4 cut(s) 48, 203, 286, 448
TasI AATT 4 cut(s) 25, 96, 415, 432
TatI WGTACW 1 cut(s) 661
TauI GCSGC 4 cut(s) 116, 124, 167, 170
TfiI GAWTC 2 cut(s) 104, 398
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 1 cut(s) 220
TseI GCWGC 5 cut(s) 55, 58, 61, 64, 124
TspDTI ATGAA 3 cut(s) 237, 270, 654
TspRI CASTG 1 cut(s) 220
VpaK11BI GGWCC 1 cut(s) 592
XapI RAATTY 1 cut(s) 25
XcmI CCANNNNNNNNNTGG 1 cut(s) 287
XhoI CTCGAG 2 cut(s) 47, 202
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.