pycom14g11700

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004996
Physical Location & Seq
Reverse (-)
14780636 .. 14781206
571 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g11700.1

Sequence Viewer

Length: 345 bp
ATGGGATTGCCATTACCGTGTGTTGAGATAGTAGCAAGCTACTGGGTTTGGAGAGTTGACTCATCCTACCGATCACAAAATCGCGACAGGGGTTGTGTTAGCCCACTTTATGTGGCGTTTTCAATCCTTATAGGTGGTCTGATGTTGGACGTGCTAATGTCCGTCACCCTCGGTGTATCTGCGCTGCCTTCTAACATTATTATAAGTATTATCGTGGTTCTTGGACTCGGAACTGCGCTGAGACTTGCCCTGAAATTCTTACACGAGTGGAGTTTGCGGAGAGCTGTGCAGAGGGTCGACGCAAGTCACACAAATGTGCCTCTTAGCTACCATCCTGCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

115

Amino Acids

12.55

Weight (kDa)

9.23

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 203
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 84
AciI CCGC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 254
AgsI TTSAA 1 cut(s) 123
AjiI CACGTC 1 cut(s) 151
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 3 cut(s) 39, 284, 327
AluI AGCT 3 cut(s) 39, 284, 327
Alw26I GTCTC 1 cut(s) 235
ApeKI GCWGC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 254
AspLEI GCGC 2 cut(s) 184, 238
AsuHPI GGTGA 1 cut(s) 157
BauI CACGAG 1 cut(s) 263
BbvI GCAGC 1 cut(s) 171
BccI CCATC 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 235
BisI GCNGC 1 cut(s) 185
BlsI GCNGC 1 cut(s) 186
BmgBI CACGTC 1 cut(s) 151
BmrI ACTGGG 1 cut(s) 52
BmuI ACTGGG 1 cut(s) 52
BoxI GACNNNNGTC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 47
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 2 cut(s) 62, 331
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 1 cut(s) 47
BseXI GCAGC 1 cut(s) 171
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 235
Bsp143I GATC 1 cut(s) 71
Bsp68I TCGCGA 1 cut(s) 84
BspACI CCGC 1 cut(s) 277
BspCNI CTCAG 1 cut(s) 231
BspFNI CGCG 1 cut(s) 84
BsrI ACTGG 1 cut(s) 47
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 1 cut(s) 71
BssSI CACGAG 1 cut(s) 263
Bst2BI CACGAG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 37
BstDEI CTNAG 2 cut(s) 239, 323
BstF5I GGATG 2 cut(s) 62, 331
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 2 cut(s) 184, 238
BstKTI GATC 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 235
BstMBI GATC 1 cut(s) 71
BstPAI GACNNNNGTC 1 cut(s) 303
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 171
BtrI CACGTC 1 cut(s) 151
BtsCI GGATG 2 cut(s) 62, 331
BtuMI TCGCGA 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 37
CfoI GCGC 2 cut(s) 184, 238
CseI GACGC 1 cut(s) 308
CviJI RGCY 4 cut(s) 39, 102, 284, 327
CviKI_1 RGCY 4 cut(s) 39, 102, 284, 327
DdeI CTNAG 2 cut(s) 239, 323
DpnI GATC 1 cut(s) 73
DpnII GATC 1 cut(s) 71
FaiI YATR 3 cut(s) 111, 131, 203
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 1 cut(s) 185
FokI GGATG 2 cut(s) 49, 318
Fsp4HI GCNGC 1 cut(s) 185
GlaI GCGC 2 cut(s) 183, 237
GluI GCNGC 1 cut(s) 185
HgaI GACGC 1 cut(s) 308
HhaI GCGC 2 cut(s) 184, 238
Hin6I GCGC 2 cut(s) 182, 236
HinP1I GCGC 2 cut(s) 182, 236
HincII GTYRAC 2 cut(s) 58, 298
HindII GTYRAC 2 cut(s) 58, 298
HinfI GANTC 2 cut(s) 59, 225
HphI GGTGA 1 cut(s) 157
Hpy166II GTNNAC 2 cut(s) 58, 298
Hpy188I TCNGA 2 cut(s) 141, 230
Hpy188III TCNNGA 1 cut(s) 83
Hpy8I GTNNAC 2 cut(s) 58, 298
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 1 cut(s) 198
HpyCH4III ACNGT 1 cut(s) 18
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 1 cut(s) 289
HpyF3I CTNAG 2 cut(s) 239, 323
HpySE526I ACGT 1 cut(s) 150
HspAI GCGC 2 cut(s) 182, 236
Kzo9I GATC 1 cut(s) 71
LpnPI CCDG 3 cut(s) 28, 73, 263
Lsp1109I GCAGC 1 cut(s) 171
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 2 cut(s) 163, 305
MalI GATC 1 cut(s) 73
MboI GATC 1 cut(s) 71
MluCI AATT 1 cut(s) 254
MlyI GAGTC 2 cut(s) 53, 219
MmeI TCCRAC 1 cut(s) 126
MnlI CCTC 3 cut(s) 179, 285, 330
MslI CAYNNNNRTG 3 cut(s) 16, 312, 314
MvnI CGCG 1 cut(s) 84
NdeII GATC 1 cut(s) 71
NmuCI GTSAC 2 cut(s) 163, 305
NruI TCGCGA 1 cut(s) 84
OliI CACNNNNGTG 1 cut(s) 314
PkrI GCNGC 1 cut(s) 186
PleI GAGTC 2 cut(s) 53, 219
PpsI GAGTC 2 cut(s) 53, 219
PshAI GACNNNNGTC 1 cut(s) 303
PsiI TTATAA 1 cut(s) 203
RruI TCGCGA 1 cut(s) 84
RseI CAYNNNNRTG 3 cut(s) 16, 312, 314
SalI GTCGAC 1 cut(s) 296
SatI GCNGC 1 cut(s) 185
Sau3AI GATC 1 cut(s) 71
SchI GAGTC 2 cut(s) 53, 219
SetI ASST 5 cut(s) 41, 136, 153, 286, 329
SmiMI CAYNNNNRTG 3 cut(s) 16, 312, 314
Sse9I AATT 1 cut(s) 254
SsiI CCGC 1 cut(s) 277
TaaI ACNGT 1 cut(s) 18
TaiI ACGT 1 cut(s) 153
TaqI TCGA 1 cut(s) 297
TasI AATT 1 cut(s) 254
TseFI GTSAC 2 cut(s) 163, 305
TseI GCWGC 1 cut(s) 184
Tsp45I GTSAC 2 cut(s) 163, 305
TspGWI ACGGA 1 cut(s) 151
XapI RAATTY 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.