AT2G46225

actin polymerization or depolymerization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004948
Physical Location & Seq
Forward (+)
18981911 .. 18984292
2382 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46225.1

Sequence Viewer

Length: 897 bp
ATGGAAACGGAGATATCAGGAATGGATAATCCAGCTATGACCTTGGACGAGGTTTCCATGGAACGCAACAAGAGCTTCGTTAAGGCATTGCAGGAGCTCAAGAACTTGAGGCCTCAACTTTATTCAGCTGCTGATTACTGTGAAAAGTCTTATCTTCATAGCGAGCAAAAGCAAATGGTATTGGACAATTTGAAAGACTACACTGTGAAGGCTCTAGTTAATGCTGTTGATCACCTTGGAACTGTAGCTTCCAAGCTTACTGATCTTTTTGATCACCAAAATTCAGACATATCAACTATGGAAATGAGAGCTTCTTGTGTTAGCCAGCAACTGCTTACATGCCGGACATATATCGACAAAGAAGGTCTTAGACAGCAACAGCTATTAGCAGTTATCCCATTGCATCACAAGCATTACATTCTTCCCAATTCTGTTAACAAGCGGGTACACTTTAGTCCTCTCAGACGAACTGACACAAGACAGAATCATTACCAAGCTATATCCCGTCTTCAACCTTCAGATGCCCCTGCATCGAAATCACTCTCCTGGCATTTAGGCTCAGAGACCAAGTCTACCCTCAAAGGAACATCCACAGTTGCACCAAGCTCCAAAGATTCAAAAGCTTTCTCAAAGACATCTGGAGTGTTCCATCTCTTAGGTGATGATGAAAACATAGCAAACAAGAAGCCTTTGGCTGGTTCTCAGGTCTCAGGTGTTCCTGCTGCATCCACTGCCCATAAGGATTTGGAGGTTCCGAAACTTCTAACTGCACACAGGTCACTTGATAACAACCCGCGACGAGAGATCATTCAAGCTCCTGTCCGGACAAAGAGTGTTCTTTCTGCGTTCTTTGTTAAACAGAAAACTCCAAAGCTCAAAGCTGGCTATGTCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.2

Weight (kDa)

9.4

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 572
AccII CGCG 1 cut(s) 796
AccIII TCCGGA 1 cut(s) 822
AciI CCGC 2 cut(s) 442, 794
AcsI RAATTY 1 cut(s) 280
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 1 cut(s) 695
AgsI TTSAA 4 cut(s) 193, 512, 618, 812
AjnI CCWGG 1 cut(s) 545
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 2 cut(s) 557, 712
AlwNI CAGNNNCTG 2 cut(s) 131, 331
Aor13HI TCCGGA 1 cut(s) 822
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 2 cut(s) 128, 722
ApoI RAATTY 1 cut(s) 280
AsuHPI GGTGA 3 cut(s) 224, 266, 671
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 892
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 115, 709
BccI CCATC 1 cut(s) 657
BciT130I CCWGG 1 cut(s) 547
BclI TGATCA 2 cut(s) 229, 271
BcoDI GTCTC 2 cut(s) 557, 712
BfaI CTAG 1 cut(s) 215
BfmI CTRYAG 1 cut(s) 243
BisI GCNGC 2 cut(s) 129, 723
BlsI GCNGC 2 cut(s) 130, 724
Bme1390I CCNGG 1 cut(s) 547
BmiI GGNNCC 1 cut(s) 753
BmrFI CCNGG 1 cut(s) 547
BmsI GCATC 4 cut(s) 412, 511, 539, 734
BpiI GAAGAC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 660
BpuEI CTTGAG 2 cut(s) 83, 127
BsaI GGTCTC 2 cut(s) 557, 712
BsaJI CCNNGG 3 cut(s) 42, 57, 235
BsaWI WCCGGW 1 cut(s) 822
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse3DI GCAATG 2 cut(s) 86, 398
BseAI TCCGGA 1 cut(s) 822
BseBI CCWGG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 42, 57, 235
BseGI GGATG 2 cut(s) 587, 725
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMI GCAATG 2 cut(s) 86, 398
BseMII CTCAG 4 cut(s) 475, 573, 716, 723
BseXI GCAGC 2 cut(s) 115, 709
BsgI GTGCAG 1 cut(s) 753
Bsh1236I CGCG 1 cut(s) 796
BshFI GGCC 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 99
BsiSI CCGG 2 cut(s) 343, 823
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 2 cut(s) 557, 712
BsnI GGCC 1 cut(s) 112
Bso31I GGTCTC 2 cut(s) 557, 712
Bsp1286I GDGCHC 1 cut(s) 99
Bsp13I TCCGGA 1 cut(s) 822
Bsp143I GATC 4 cut(s) 229, 262, 271, 804
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 2 cut(s) 442, 794
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 4 cut(s) 474, 572, 715, 722
BspEI TCCGGA 1 cut(s) 822
BspFNI CGCG 1 cut(s) 796
BspLI GGNNCC 1 cut(s) 753
BspTNI GGTCTC 2 cut(s) 557, 712
BsrDI GCAATG 2 cut(s) 86, 398
BssECI CCNNGG 3 cut(s) 42, 57, 235
BssMI GATC 4 cut(s) 229, 262, 271, 804
BssSI CACGAG 1 cut(s) 892
BssT1I CCWWGG 3 cut(s) 42, 57, 235
Bst2BI CACGAG 1 cut(s) 892
Bst2UI CCWGG 1 cut(s) 547
Bst4CI ACNGT 4 cut(s) 140, 205, 244, 595
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 164, 326, 883
BstDEI CTNAG 6 cut(s) 368, 461, 559, 655, 702, 709
BstDSI CCRYGG 1 cut(s) 57
BstF5I GGATG 2 cut(s) 587, 725
BstFNI CGCG 1 cut(s) 796
BstKTI GATC 4 cut(s) 232, 265, 274, 807
BstMAI GTCTC 2 cut(s) 557, 712
BstMBI GATC 4 cut(s) 229, 262, 271, 804
BstMWI GCNNNNNNNGC 3 cut(s) 72, 409, 731
BstNI CCWGG 1 cut(s) 547
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 545
BstSFI CTRYAG 1 cut(s) 243
BstUI CGCG 1 cut(s) 796
BstV1I GCAGC 2 cut(s) 115, 709
BstV2I GAAGAC 1 cut(s) 500
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 587, 725
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 2 cut(s) 201, 729
Cac8I GCNNGC 3 cut(s) 164, 326, 883
CaiI CAGNNNCTG 2 cut(s) 131, 331
Csp6I GTAC 1 cut(s) 446
CviAII CATG 2 cut(s) 58, 339
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 6 cut(s) 368, 461, 559, 655, 702, 709
DpnI GATC 4 cut(s) 231, 264, 273, 806
DpnII GATC 4 cut(s) 229, 262, 271, 804
Ecl136II GAGCTC 1 cut(s) 97
Eco130I CCWWGG 3 cut(s) 42, 57, 235
Eco147I AGGCCT 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 2 cut(s) 557, 712
Eco32I GATATC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 97
Eco57I CTGAAG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 97
EcoRII CCWGG 1 cut(s) 545
EcoRV GATATC 1 cut(s) 15
EcoT14I CCWWGG 3 cut(s) 42, 57, 235
EcoT38I GRGCYC 1 cut(s) 99
ErhI CCWWGG 3 cut(s) 42, 57, 235
FaeI CATG 2 cut(s) 61, 342
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 2 cut(s) 57, 338
FauI CCCGC 2 cut(s) 435, 801
FbaI TGATCA 2 cut(s) 229, 271
FblI GTMKAC 1 cut(s) 572
Fnu4HI GCNGC 2 cut(s) 129, 723
FokI GGATG 2 cut(s) 574, 712
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 2 cut(s) 129, 723
FspBI CTAG 1 cut(s) 215
GluI GCNGC 2 cut(s) 129, 723
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 1 cut(s) 112
HapII CCGG 2 cut(s) 343, 823
Hin1II CATG 2 cut(s) 61, 342
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 254, 621
HinfI GANTC 2 cut(s) 484, 614
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 2 cut(s) 343, 823
HphI GGTGA 3 cut(s) 224, 266, 671
Hpy166II GTNNAC 3 cut(s) 436, 448, 573
Hpy188I TCNGA 5 cut(s) 286, 464, 520, 562, 756
Hpy188III TCNNGA 5 cut(s) 18, 100, 639, 823, 894
Hpy8I GTNNAC 3 cut(s) 436, 448, 573
Hpy99I CGWCG 1 cut(s) 801
HpyAV CCTTC 3 cut(s) 202, 356, 525
HpyCH4III ACNGT 4 cut(s) 140, 205, 244, 595
HpyCH4V TGCA 6 cut(s) 91, 403, 530, 599, 725, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 72, 409, 731
HpyF3I CTNAG 6 cut(s) 368, 461, 559, 655, 702, 709
Hsp92II CATG 2 cut(s) 61, 342
Kpn2I TCCGGA 1 cut(s) 822
Ksp22I TGATCA 2 cut(s) 229, 271
KspAI GTTAAC 1 cut(s) 436
Kzo9I GATC 4 cut(s) 229, 262, 271, 804
LmnI GCTCC 3 cut(s) 94, 611, 820
Lsp1109I GCAGC 2 cut(s) 115, 709
LweI GCATC 4 cut(s) 412, 511, 539, 734
MaeI CTAG 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 777
MalI GATC 4 cut(s) 231, 264, 273, 806
MboI GATC 4 cut(s) 229, 262, 271, 804
MboII GAAGA 3 cut(s) 146, 413, 500
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 3 cut(s) 187, 280, 427
MnlI CCTC 6 cut(s) 43, 102, 123, 468, 587, 742
MroI TCCGGA 1 cut(s) 822
MseI TTAA 4 cut(s) 81, 219, 435, 855
MspA1I CMGCKG 1 cut(s) 128
MspI CCGG 2 cut(s) 343, 823
MspR9I CCNGG 1 cut(s) 547
MvaI CCWGG 1 cut(s) 547
MvnI CGCG 1 cut(s) 796
MwoI GCNNNNNNNGC 3 cut(s) 72, 409, 731
NcoI CCATGG 1 cut(s) 57
NdeII GATC 4 cut(s) 229, 262, 271, 804
NlaIII CATG 2 cut(s) 61, 342
NlaIV GGNNCC 1 cut(s) 753
NmuCI GTSAC 1 cut(s) 777
NspI RCATGY 1 cut(s) 342
PceI AGGCCT 1 cut(s) 112
PfeI GAWTC 2 cut(s) 484, 614
PflFI GACNNNGTC 1 cut(s) 568
PkrI GCNGC 2 cut(s) 130, 724
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 545
PspGI CCWGG 1 cut(s) 545
PspN4I GGNNCC 1 cut(s) 753
PstNI CAGNNNCTG 2 cut(s) 131, 331
PsyI GACNNNGTC 1 cut(s) 568
PvuII CAGCTG 1 cut(s) 128
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 4 cut(s) 81, 219, 435, 855
SatI GCNGC 2 cut(s) 129, 723
Sau3AI GATC 4 cut(s) 229, 262, 271, 804
ScrFI CCNGG 1 cut(s) 547
SduI GDGCHC 1 cut(s) 99
SfaNI GCATC 4 cut(s) 412, 511, 539, 734
SfcI CTRYAG 1 cut(s) 243
SmlI CTYRAG 2 cut(s) 98, 106
SmoI CTYRAG 2 cut(s) 98, 106
Sse9I AATT 3 cut(s) 187, 280, 427
SseBI AGGCCT 1 cut(s) 112
SsiI CCGC 2 cut(s) 442, 794
SspMI CTAG 1 cut(s) 215
SstI GAGCTC 1 cut(s) 99
StuI AGGCCT 1 cut(s) 112
StyD4I CCNGG 1 cut(s) 545
StyI CCWWGG 3 cut(s) 42, 57, 235
TaaI ACNGT 4 cut(s) 140, 205, 244, 595
TaqI TCGA 2 cut(s) 354, 533
TasI AATT 3 cut(s) 187, 280, 427
TfiI GAWTC 2 cut(s) 484, 614
Tru1I TTAA 4 cut(s) 81, 219, 435, 855
Tru9I TTAA 4 cut(s) 81, 219, 435, 855
TscAI CASTG 2 cut(s) 208, 736
TseFI GTSAC 1 cut(s) 777
TseI GCWGC 2 cut(s) 128, 722
Tsp45I GTSAC 1 cut(s) 777
TspDTI ATGAA 2 cut(s) 146, 681
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 2 cut(s) 208, 736
Tth111I GACNNNGTC 1 cut(s) 568
XapI RAATTY 1 cut(s) 280
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 1 cut(s) 572
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.