AT2G46810
BHLH Family

basic helix-loop-helix (bHLH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003385
Physical Location & Seq
Forward (+)
19239694 .. 19242772
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46810.3

Sequence Viewer

Length: 1116 bp
ATGTTTGTCCTGAGAGTAAGTAACCAGAGCTTTAAGTTACATCAGCAAGTACAATGTAAAGATGAAATCTTCTGTTTGGACCAAAAAGTGAATGTGAGAAGAAGCCTACAAGTTCAAGAAACTGTAGAGGATCATCAAAGCTTTGCCCTTGAAGAGGAAGAACAACAACTCTCAACTCCGAGCTTGCTGCAAGACACAACAATACCATTTCTACAAATGCTGCAACAAAGTGAAGACCCTTCACCGTTTTTGTCATTCAAAGACCCAAGCTTTCTAGCACTACTATCTCTCCAGACACTTGAAAAGCCTTGGGAACTCGAAAACTACCTCCCACATGAAGTTCCAGAGTTTCATTCACCGATCCATTCTGAAACCAACCACTACTATCATAATCCATCTTTGGAAGGAGTCAATGAAGCCATCTCAAACCAAGAACTTCCATTCAACCCACTAGAGAATGCGCGTTCAAGACGCAAGCGGAAAAACAACAACTTGGCATCATTGATGACAAGAGAAAAGCGAAAGAGAAGAAGAACTAAACCAACAAAGAACATAGAAGAGATAGAGAGTCAAAGAATGACACACATTGCGGTTGAACGAAACCGCAGACGCCAAATGAACGTTCATCTGAACTCACTCCGCTCCATCATTCCATCTTCATACATCCAGAGGGGAGACCAAGCGTCAATAGTAGGAGGAGCAATAGACTTCGTAAAGATCCTAGAGCAACAGTTGCAATCCCTTGAAGCACAAAAGAGAAGTCAACAGAGTGATGATAACAAAGAGCAAATTCCAGAAGATAACAGTCTCAGGAACATTTCGTCGAACAAGTTGCGTGCGAGTAATAAAGAAGAACAAAGTAGCAAACTCAAAATCGAAGCCACAGTGATAGAGAGTCACGTCAACCTAAAAATTCAATGTACGAGGAAACAAGGACAACTTCTCAGATCAATCATATTGCTGGAGAAACTTCGATTCACTGTTCTTCATCTCAACATCACATCTCCGACCAATACATCTGTCTCTTATTCCTTCAACCTCAAGATGGAAGATGAATGTAATTTGGGATCAGCGGATGAGATAACGGCGGCGATTCGTCAGATTTTCGACAGCTGA

Protein Analysis

371

Amino Acids

43.0

Weight (kDa)

6.85

Isoelectric Point (pI)

87.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 179 - 229 2.2e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 642
AccII CGCG 1 cut(s) 463
AciI CCGC 6 cut(s) 478, 590, 604, 640, 1073, 1088
AclI AACGTT 1 cut(s) 621
AclWI GGATC 4 cut(s) 138, 355, 712, 1075
AcsI RAATTY 2 cut(s) 789, 912
AcyI GRCGYC 1 cut(s) 610
AfaI GTAC 2 cut(s) 51, 922
AfiI CCNNNNNNNGG 2 cut(s) 154, 1045
AhdI GACNNNNNGTC 1 cut(s) 682
AjiI CACGTC 1 cut(s) 901
AjuI GAANNNNNNNTTGG 2 cut(s) 606, 638
AluBI AGCT 5 cut(s) 30, 141, 183, 270, 1113
AluI AGCT 5 cut(s) 30, 141, 183, 270, 1113
Alw26I GTCTC 3 cut(s) 669, 812, 1027
AlwI GGATC 4 cut(s) 138, 355, 712, 1075
ApeKI GCWGC 2 cut(s) 187, 220
ApoI RAATTY 2 cut(s) 789, 912
ArsI GACNNNNNNTTYG 2 cut(s) 745, 777
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 79
AsuHPI GGTGA 2 cut(s) 234, 348
AvaII GGWCC 1 cut(s) 79
BbsI GAAGAC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 174, 207
BccI CCATC 5 cut(s) 403, 428, 653, 661, 1039
BceAI ACGGC 1 cut(s) 1101
BcgI CGANNNNNNTGC 4 cut(s) 169, 203, 814, 848
BcoDI GTCTC 3 cut(s) 669, 812, 1027
BfaI CTAG 3 cut(s) 275, 452, 722
BfmI CTRYAG 1 cut(s) 123
BisI GCNGC 3 cut(s) 188, 221, 1089
BlsI GCNGC 3 cut(s) 189, 222, 1090
Bme18I GGWCC 1 cut(s) 79
BmeRI GACNNNNNGTC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 901
BmgT120I GGNCC 1 cut(s) 79
BmsI GCATC 1 cut(s) 506
BpiI GAAGAC 1 cut(s) 240
BpmI CTGGAG 2 cut(s) 275, 983
BpuEI CTTGAG 1 cut(s) 1025
BsaHI GRCGYC 1 cut(s) 610
BsaI GGTCTC 1 cut(s) 669
BsaJI CCNNGG 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 154, 1045
Bse3DI GCAATG 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 2 cut(s) 663, 1081
BseLI CCNNNNNNNGG 2 cut(s) 154, 1045
BseMI GCAATG 1 cut(s) 585
BseMII CTCAG 2 cut(s) 823, 958
BseRI GAGGAG 1 cut(s) 711
BseXI GCAGC 2 cut(s) 174, 207
Bsh1236I CGCG 1 cut(s) 463
BslI CCNNNNNNNGG 2 cut(s) 154, 1045
BsmAI GTCTC 3 cut(s) 669, 812, 1027
BsmI GAATGC 1 cut(s) 463
Bso31I GGTCTC 1 cut(s) 669
Bsp143I GATC 5 cut(s) 130, 360, 717, 947, 1067
BspACI CCGC 6 cut(s) 478, 590, 604, 640, 1073, 1088
BspCNI CTCAG 2 cut(s) 822, 957
BspFNI CGCG 1 cut(s) 463
BspPI GGATC 4 cut(s) 138, 355, 712, 1075
BspTNI GGTCTC 1 cut(s) 669
BsrBI CCGCTC 1 cut(s) 642
BsrDI GCAATG 1 cut(s) 585
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 5 cut(s) 130, 360, 717, 947, 1067
BssNI GRCGYC 1 cut(s) 610
BssT1I CCWWGG 1 cut(s) 308
Bst4CI ACNGT 6 cut(s) 124, 246, 732, 806, 886, 982
Bst6I CTCTTC 2 cut(s) 147, 552
BstACI GRCGYC 1 cut(s) 610
BstAPI GCANNNNNTGC 1 cut(s) 733
BstC8I GCNNGC 3 cut(s) 185, 476, 837
BstDEI CTNAG 3 cut(s) 11, 809, 944
BstENI CCTNNNNNAGG 1 cut(s) 152
BstF5I GGATG 2 cut(s) 663, 1081
BstFNI CGCG 1 cut(s) 463
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 5 cut(s) 133, 363, 720, 950, 1070
BstMAI GTCTC 3 cut(s) 669, 812, 1027
BstMBI GATC 5 cut(s) 130, 360, 717, 947, 1067
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstSFI CTRYAG 1 cut(s) 123
BstUI CGCG 1 cut(s) 463
BstV1I GCAGC 2 cut(s) 174, 207
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 717
BstYI RGATCY 1 cut(s) 717
BtrI CACGTC 1 cut(s) 901
BtsCI GGATG 2 cut(s) 663, 1081
BtsIMutI CAGTG 2 cut(s) 891, 978
Cac8I GCNNGC 3 cut(s) 185, 476, 837
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 79
CseI GACGC 3 cut(s) 480, 618, 672
Csp6I GTAC 2 cut(s) 50, 921
CviAII CATG 1 cut(s) 335
CviJI RGCY 9 cut(s) 30, 105, 141, 183, 270, 307, 419, 881, 1113
CviKI_1 RGCY 9 cut(s) 30, 105, 141, 183, 270, 307, 419, 881, 1113
CviQI GTAC 2 cut(s) 50, 921
DdeI CTNAG 3 cut(s) 11, 809, 944
DpnI GATC 5 cut(s) 132, 362, 719, 949, 1069
DpnII GATC 5 cut(s) 130, 360, 717, 947, 1067
DriI GACNNNNNGTC 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 147, 552
Eam1105I GACNNNNNGTC 1 cut(s) 682
EarI CTCTTC 2 cut(s) 147, 552
Eco130I CCWWGG 1 cut(s) 308
Eco31I GGTCTC 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 79
EcoNI CCTNNNNNAGG 1 cut(s) 152
EcoT14I CCWWGG 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 308
FaeI CATG 1 cut(s) 338
FaiI YATR 5 cut(s) 336, 390, 554, 661, 956
FalI AAGNNNNNCTT 2 cut(s) 924, 956
FatI CATG 1 cut(s) 334
Fnu4HI GCNGC 3 cut(s) 188, 221, 1089
FokI GGATG 2 cut(s) 650, 1088
Fsp4HI GCNGC 3 cut(s) 188, 221, 1089
FspBI CTAG 3 cut(s) 275, 452, 722
GlaI GCGC 1 cut(s) 462
GluI GCNGC 3 cut(s) 188, 221, 1089
GsuI CTGGAG 2 cut(s) 275, 983
HgaI GACGC 3 cut(s) 480, 618, 672
HhaI GCGC 1 cut(s) 463
Hin1I GRCGYC 1 cut(s) 610
Hin1II CATG 1 cut(s) 338
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 2 cut(s) 764, 904
HindII GTYRAC 2 cut(s) 764, 904
HindIII AAGCTT 2 cut(s) 139, 268
HinfI GANTC 5 cut(s) 408, 568, 895, 975, 1093
HphI GGTGA 2 cut(s) 234, 348
Hpy166II GTNNAC 2 cut(s) 764, 904
Hpy188I TCNGA 6 cut(s) 180, 370, 630, 947, 1008, 1101
Hpy188III TCNNGA 9 cut(s) 10, 116, 292, 344, 468, 667, 794, 811, 1042
Hpy8I GTNNAC 2 cut(s) 764, 904
Hpy99I CGWCG 1 cut(s) 826
HpyAV CCTTC 3 cut(s) 249, 398, 1042
HpyCH4III ACNGT 6 cut(s) 124, 246, 732, 806, 886, 982
HpyCH4IV ACGT 2 cut(s) 621, 900
HpyCH4V TGCA 3 cut(s) 190, 223, 736
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpyF3I CTNAG 3 cut(s) 11, 809, 944
HpySE526I ACGT 2 cut(s) 621, 900
Hsp92I GRCGYC 1 cut(s) 610
Hsp92II CATG 1 cut(s) 338
HspAI GCGC 1 cut(s) 461
Kzo9I GATC 5 cut(s) 130, 360, 717, 947, 1067
LmnI GCTCC 2 cut(s) 647, 698
LpnPI CCDG 8 cut(s) 23, 38, 305, 357, 680, 796, 807, 947
Lsp1109I GCAGC 2 cut(s) 174, 207
LweI GCATC 1 cut(s) 506
MaeI CTAG 3 cut(s) 275, 452, 722
MaeII ACGT 2 cut(s) 621, 900
MaeIII GTNAC 3 cut(s) 20, 36, 896
MalI GATC 5 cut(s) 132, 362, 719, 949, 1069
MbiI CCGCTC 1 cut(s) 642
MboI GATC 5 cut(s) 130, 360, 717, 947, 1067
MflI RGATCY 1 cut(s) 717
MluCI AATT 3 cut(s) 789, 912, 1060
MlyI GAGTC 3 cut(s) 417, 577, 904
MmeI TCCRAC 1 cut(s) 1031
MnlI CCTC 7 cut(s) 121, 148, 338, 663, 689, 918, 1049
MseI TTAA 1 cut(s) 33
MspA1I CMGCKG 2 cut(s) 1073, 1113
Mva1269I GAATGC 1 cut(s) 463
MvnI CGCG 1 cut(s) 463
MwoI GCNNNNNNNGC 1 cut(s) 733
NdeII GATC 5 cut(s) 130, 360, 717, 947, 1067
NlaIII CATG 1 cut(s) 338
NmuCI GTSAC 1 cut(s) 896
PctI GAATGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 975, 1093
PkrI GCNGC 3 cut(s) 189, 222, 1090
PleI GAGTC 3 cut(s) 416, 576, 903
PpsI GAGTC 3 cut(s) 416, 576, 903
Psp1406I AACGTT 1 cut(s) 621
PspPI GGNCC 1 cut(s) 79
PsuI RGATCY 1 cut(s) 717
PvuII CAGCTG 1 cut(s) 1113
RsaI GTAC 2 cut(s) 51, 922
RsaNI GTAC 2 cut(s) 50, 921
SaqAI TTAA 1 cut(s) 33
SatI GCNGC 3 cut(s) 188, 221, 1089
Sau3AI GATC 5 cut(s) 130, 360, 717, 947, 1067
Sau96I GGNCC 1 cut(s) 79
SchI GAGTC 3 cut(s) 417, 577, 904
SfaNI GCATC 1 cut(s) 506
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 1040
SmoI CTYRAG 1 cut(s) 1040
Sse9I AATT 3 cut(s) 789, 912, 1060
SsiI CCGC 6 cut(s) 478, 590, 604, 640, 1073, 1088
SspMI CTAG 3 cut(s) 275, 452, 722
StyI CCWWGG 1 cut(s) 308
TaaI ACNGT 6 cut(s) 124, 246, 732, 806, 886, 982
TaiI ACGT 2 cut(s) 624, 903
TaqI TCGA 5 cut(s) 318, 824, 876, 973, 1107
TasI AATT 3 cut(s) 789, 912, 1060
TatI WGTACW 1 cut(s) 49
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 2 cut(s) 975, 1093
Tru1I TTAA 1 cut(s) 33
Tru9I TTAA 1 cut(s) 33
TscAI CASTG 2 cut(s) 891, 985
TseFI GTSAC 1 cut(s) 896
TseI GCWGC 2 cut(s) 187, 220
Tsp45I GTSAC 1 cut(s) 896
TspDTI ATGAA 9 cut(s) 78, 341, 351, 429, 614, 632, 648, 977, 1068
TspRI CASTG 2 cut(s) 891, 985
VpaK11BI GGWCC 1 cut(s) 79
XagI CCTNNNNNAGG 1 cut(s) 152
XapI RAATTY 2 cut(s) 789, 912
XspI CTAG 3 cut(s) 275, 452, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.