Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0003385

Physical Location & Seq

Genomic Coordinates Forward (+)

20236399 .. 20239050

Gene Structure 2652 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1089300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGGAGAGGCTTCTTCAAGGACCCATTAATCCCTGTCAGTTCTTTGGTGAGCATTTGGATTTTCCGTTCGAGCAAAGTTTGAATTTCACCACAGAAACCTTGAGATTTGAGGAGGAAGAAGAATCCGACCACCCCCACCTCTCATTGCCAAGCTTGGATGACAGAATGCCATTTCTCCAGATGCTACAAAGTGTTGATTCCTCCACCACCACCACCACACCTTATTTTCCATTGAAAGAGCCAAGCTTTCAAGCACTTTTGAAACTCCAACACTTCAAAAAACCATGGGAGTTGGATAAAAATTACATGCCTGAAATGGAAACCCCAATTCAGAAAGCTCTGGAGCTTGAGAGCTGTGTGACCCATGCAATGGTTGAGCTGCAACATTCACCTGTTAAATCAGAAGGGAAAGACTCTCATCTCCACCATTCTATTTCTGAGTGCAACCAAGGGGAAGAAAAACCCAGCTCAGTTGCTGGGTCTCCACCACCACCTACCTGGATTCAGGTACAAAATGGTGCTGAGAAAACCCAGCAACCCAAATCTACTCCGGCCACCAGGGAGAGGAGAAAGCGGAAGAGAACAAGGCCAACCAAGAACAAGGAAGAAGTGGAGAGCCAGCGGATGACCCACATTGCGGTGGAGCGCAACCGCCGCCGCCAGATGAATGACCATCTCAATGTCCTCAGGTCCCTCATGCCCACTTCCTACATTCAAAGGGGTGACCAAGCATCAATTGTTGGGGGTGCAATAGATTTTGTGAAGGAATTGGAGCAGCTACTTCATTCCCTTGAAGCCCAAAAGAGAACGAGAAAAGCAGCTGATCAAATCCAAGGGATGAGCAATATTGGTGACAACAACAGCTCTTTCAGCTCCTCATCAGCATCCTCCTCTTCTCCCTGCAGCATGGCAATGCCATCCAATGGAATGTTCATGAGTCTCTCCCAATGCAGAATTGGGTCTCATGATCAAGAAAGCACTACCGCCACCTTCGACGATGAAGGCACTGCTCAGAACAAGTCTGAGGCTGCAGACATAGATGTCACTGTGATCCAATCCCATGTCAACTTAAAGGTGAAGTGCCAAAGGAGAGCTGGACAGCTGATGGATGCCATTGTTGCACTGGAAGATCTTAGGCTAACTGTTTTGCACCTCAACATTACATCTTCACAAGACACTGTTCTTTACTCTTTCAATCTCGAGATAGAAGAAGGTTGTAATTTAGGATCAGCAGATGAAATTGCAAAAGCAGTACATCAAATATTCAGCTTCATCAATGGTAGCAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH

Protein Analysis

430

Amino Acids

48.17

Weight (kDa)

5.62

Isoelectric Point (pI)

63.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	207 - 258	1.3e-12	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	346 - 422	7.3e-09	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0003385)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46810 AT2G46810 AT2G46810 AT2G46810 AT3G61950 AT3G61950 AT3G61950 AT3G61950 AT3G61950 AT3G61950 AT4G01460 AT4G01460
fragaria_vesca	FvH4_7g13190 FvH4_7g13190 FvH4_7g13190 FvH4_7g13190 FvH4_7g13190 FvH4_7g17790 FvH4_7g17790 FvH4_7g17790
malus_domestica	MD01G1089300.v1.1 MD07G1160700.v1.1
prunus_persica	Prupe.2G198800_v2.0.a1 Prupe.2G198800_v2.0.a1
pyrus_communis	pycom01g11350 pycom07g15680
rosa_chinensis	RchiOBHm_Chr1g0361191
rosa_laevigata	RLG00000027740
rosa_multiflora	Rmu_sc0007111.1_g000017
rosa_roxburghii	Rroxscaffold_4G00295280
rosa_rugosa	Rorug01G0287400.1
rosa_samantha	Rh1AG298300 Rh1BG262200 Rh1CG279600 Rh1DG292400
rosa_wichuraiana	Rw1G026370

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1040
AccB7I	CCANNNNNTGG	2 cut(s)	370, 923
AciI	CCGC	7 cut(s)	574, 622, 638, 652, 655, 658, 984
AclWI	GGATC	2 cut(s)	1045, 1234
AcoI	YGGCCR	1 cut(s)	552
AcsI	RAATTY	1 cut(s)	82
AfaI	GTAC	2 cut(s)	510, 1254
AfiI	CCNNNNNNNGG	4 cut(s)	370, 564, 637, 923
AgsI	TTSAA	9 cut(s)	17, 82, 235, 251, 262, 277, 716, 794, 1195
AjnI	CCWGG	2 cut(s)	497, 557
AjuI	GAANNNNNNNTTGG	2 cut(s)	914, 946
AloI	GAACNNNNNNTCC	2 cut(s)	50, 82
Alw26I	GTCTC	3 cut(s)	486, 944, 966
AlwI	GGATC	2 cut(s)	1045, 1234
AlwNI	CAGNNNCTG	1 cut(s)	476
Ama87I	CYCGRG	1 cut(s)	1199
AoxI	GGCC	2 cut(s)	552, 587
ApeKI	GCWGC	5 cut(s)	379, 775, 818, 903, 1028
ApoI	RAATTY	1 cut(s)	82
AseI	ATTAAT	1 cut(s)	27
AspLEI	GCGC	1 cut(s)	648
AspS9I	GGNCC	2 cut(s)	20, 690
AsuHPI	GGTGA	6 cut(s)	59, 79, 381, 734, 863, 1087
AvaI	CYCGRG	1 cut(s)	1199
AvaII	GGWCC	2 cut(s)	20, 690
AxyI	CCTNAGG	1 cut(s)	686
BbvI	GCAGC	5 cut(s)	366, 787, 830, 915, 1015
BccI	CCATC	3 cut(s)	681, 925, 1099
BciT130I	CCWGG	2 cut(s)	499, 559
BclI	TGATCA	2 cut(s)	823, 967
BcoDI	GTCTC	3 cut(s)	486, 944, 966
BfmI	CTRYAG	2 cut(s)	901, 1029
BglII	AGATCT	1 cut(s)	1129
BisI	GCNGC	7 cut(s)	380, 655, 658, 776, 819, 904, 1029
BlsI	GCNGC	7 cut(s)	381, 656, 659, 777, 820, 905, 1030
Bme1390I	CCNGG	2 cut(s)	499, 559
Bme18I	GGWCC	2 cut(s)	20, 690
BmeT110I	CYCGRG	1 cut(s)	1199
BmgT120I	GGNCC	2 cut(s)	20, 690
BmiI	GGNNCC	2 cut(s)	22, 692
BmrFI	CCNGG	2 cut(s)	499, 559
BmsI	GCATC	4 cut(s)	171, 740, 893, 1099
BpmI	CTGGAG	2 cut(s)	161, 362
BpuEI	CTTGAG	2 cut(s)	121, 368
BsaI	GGTCTC	2 cut(s)	486, 966
BsaJI	CCNNGG	4 cut(s)	284, 448, 558, 832
Bsc4I	CCNNNNNNNGG	4 cut(s)	370, 564, 637, 923
Bse1I	ACTGG	1 cut(s)	1128
Bse21I	CCTNAGG	1 cut(s)	686
Bse3DI	GCAATG	4 cut(s)	143, 375, 633, 918
BseBI	CCWGG	2 cut(s)	499, 559
BseDI	CCNNGG	4 cut(s)	284, 448, 558, 832
BseGI	GGATG	6 cut(s)	163, 630, 843, 884, 917, 1114
BseLI	CCNNNNNNNGG	4 cut(s)	370, 564, 637, 923
BseMI	GCAATG	4 cut(s)	143, 375, 633, 918
BseMII	CTCAG	6 cut(s)	429, 483, 513, 700, 1014, 1025
BseNI	ACTGG	1 cut(s)	1128
BseRI	GAGGAG	4 cut(s)	125, 580, 865, 880
BseXI	GCAGC	5 cut(s)	366, 787, 830, 915, 1015
BseYI	CCCAGC	3 cut(s)	464, 476, 531
BshFI	GGCC	2 cut(s)	554, 589
BsiHKCI	CYCGRG	1 cut(s)	1199
BsiSI	CCGG	1 cut(s)	551
BslFI	GGGAC	1 cut(s)	676
BslI	CCNNNNNNNGG	4 cut(s)	370, 564, 637, 923
BsmAI	GTCTC	3 cut(s)	486, 944, 966
BsmFI	GGGAC	1 cut(s)	676
BsmI	GAATGC	1 cut(s)	171
BsnI	GGCC	2 cut(s)	554, 589
Bso31I	GGTCTC	2 cut(s)	486, 966
BsoBI	CYCGRG	1 cut(s)	1199
Bsp143I	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
Bsp19I	CCATGG	1 cut(s)	284
BspACI	CCGC	7 cut(s)	574, 622, 638, 652, 655, 658, 984
BspANI	GGCC	2 cut(s)	554, 589
BspCNI	CTCAG	6 cut(s)	430, 482, 514, 699, 1015, 1024
BspHI	TCATGA	2 cut(s)	933, 964
BspLI	GGNNCC	2 cut(s)	22, 692
BspMAI	CTGCAG	2 cut(s)	905, 1033
BspPI	GGATC	2 cut(s)	1045, 1234
BspTNI	GGTCTC	2 cut(s)	486, 966
BsrDI	GCAATG	4 cut(s)	143, 375, 633, 918
BsrI	ACTGG	1 cut(s)	1128
BssECI	CCNNGG	4 cut(s)	284, 448, 558, 832
BssMI	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
BssT1I	CCWWGG	3 cut(s)	284, 448, 832
Bst2UI	CCWGG	2 cut(s)	499, 559
Bst4CI	ACNGT	3 cut(s)	1048, 1144, 1180
Bst6I	CTCTTC	2 cut(s)	572, 898
BstC8I	GCNNGC	1 cut(s)	620
BstDEI	CTNAG	7 cut(s)	438, 469, 522, 686, 1011, 1023, 1133
BstDSI	CCRYGG	1 cut(s)	284
BstEII	GGTNACC	1 cut(s)	722
BstF5I	GGATG	6 cut(s)	163, 630, 843, 884, 917, 1114
BstHHI	GCGC	1 cut(s)	648
BstKTI	GATC	5 cut(s)	826, 970, 1053, 1132, 1229
BstMAI	GTCTC	3 cut(s)	486, 944, 966
BstMBI	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
BstMWI	GCNNNNNNNGC	3 cut(s)	654, 870, 1118
BstNI	CCWGG	2 cut(s)	499, 559
BstNSI	RCATGY	1 cut(s)	310
BstPI	GGTNACC	1 cut(s)	722
BstSCI	CCNGG	2 cut(s)	497, 557
BstSFI	CTRYAG	2 cut(s)	901, 1029
BstV1I	GCAGC	5 cut(s)	366, 787, 830, 915, 1015
BstX2I	RGATCY	1 cut(s)	1129
BstXI	CCANNNNNNTGG	1 cut(s)	498
BstYI	RGATCY	1 cut(s)	1129
Bsu36I	CCTNAGG	1 cut(s)	686
BsuRI	GGCC	2 cut(s)	554, 589
BtgI	CCRYGG	1 cut(s)	284
BtsCI	GGATG	6 cut(s)	163, 630, 843, 884, 917, 1114
BtsI	GCAGTG	1 cut(s)	1005
BtsIMutI	CAGTG	4 cut(s)	1005, 1044, 1121, 1176
Cac8I	GCNNGC	1 cut(s)	620
CaiI	CAGNNNCTG	1 cut(s)	476
CciI	TCATGA	2 cut(s)	933, 964
CfoI	GCGC	1 cut(s)	648
Cfr13I	GGNCC	2 cut(s)	20, 690
Csp6I	GTAC	2 cut(s)	509, 1253
CviAII	CATG	8 cut(s)	285, 307, 365, 697, 907, 934, 965, 1061
CviQI	GTAC	2 cut(s)	509, 1253
DdeI	CTNAG	7 cut(s)	438, 469, 522, 686, 1011, 1023, 1133
DpnI	GATC	5 cut(s)	825, 969, 1052, 1131, 1228
DpnII	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
DrdI	GACNNNNNNGTC	1 cut(s)	1040
DseDI	GACNNNNNNGTC	1 cut(s)	1040
EaeI	YGGCCR	1 cut(s)	552
Eam1104I	CTCTTC	2 cut(s)	572, 898
EarI	CTCTTC	2 cut(s)	572, 898
Eco130I	CCWWGG	3 cut(s)	284, 448, 832
Eco31I	GGTCTC	2 cut(s)	486, 966
Eco47I	GGWCC	2 cut(s)	20, 690
Eco81I	CCTNAGG	1 cut(s)	686
Eco88I	CYCGRG	1 cut(s)	1199
Eco91I	GGTNACC	1 cut(s)	722
EcoO109I	RGGNCCY	2 cut(s)	20, 690
EcoO65I	GGTNACC	1 cut(s)	722
EcoRII	CCWGG	2 cut(s)	497, 557
EcoT14I	CCWWGG	3 cut(s)	284, 448, 832
ErhI	CCWWGG	3 cut(s)	284, 448, 832
FaeI	CATG	8 cut(s)	288, 310, 368, 700, 910, 937, 968, 1064
FaiI	YATR	9 cut(s)	286, 308, 366, 698, 908, 935, 966, 1037, 1062
FaqI	GGGAC	1 cut(s)	676
FatI	CATG	8 cut(s)	284, 306, 364, 696, 906, 933, 964, 1060
FbaI	TGATCA	2 cut(s)	823, 967
Fnu4HI	GCNGC	7 cut(s)	380, 655, 658, 776, 819, 904, 1029
FokI	GGATG	6 cut(s)	170, 637, 850, 871, 904, 1121
Fsp4HI	GCNGC	7 cut(s)	380, 655, 658, 776, 819, 904, 1029
GlaI	GCGC	1 cut(s)	647
GluI	GCNGC	7 cut(s)	380, 655, 658, 776, 819, 904, 1029
GsaI	CCCAGC	3 cut(s)	468, 480, 535
GsuI	CTGGAG	2 cut(s)	161, 362
HaeIII	GGCC	2 cut(s)	554, 589
HapII	CCGG	1 cut(s)	551
HhaI	GCGC	1 cut(s)	648
Hin1II	CATG	8 cut(s)	288, 310, 368, 700, 910, 937, 968, 1064
Hin6I	GCGC	1 cut(s)	646
HinP1I	GCGC	1 cut(s)	646
HincII	GTYRAC	1 cut(s)	1066
HindII	GTYRAC	1 cut(s)	1066
HindIII	AAGCTT	2 cut(s)	151, 244
HinfI	GANTC	5 cut(s)	122, 197, 413, 502, 937
HpaII	CCGG	1 cut(s)	551
HphI	GGTGA	6 cut(s)	59, 79, 381, 734, 863, 1087
Hpy166II	GTNNAC	1 cut(s)	1066
Hpy188I	TCNGA	6 cut(s)	127, 333, 403, 439, 1014, 1024
Hpy188III	TCNNGA	7 cut(s)	178, 341, 934, 965, 971, 1199, 1201
Hpy8I	GTNNAC	1 cut(s)	1066
Hpy99I	CGWCG	1 cut(s)	998
HpyAV	CCTTC	5 cut(s)	398, 757, 995, 1000, 1205
HpyCH4III	ACNGT	3 cut(s)	1048, 1144, 1180
HpyF10VI	GCNNNNNNNGC	3 cut(s)	654, 870, 1118
HpyF3I	CTNAG	7 cut(s)	438, 469, 522, 686, 1011, 1023, 1133
Hsp92II	CATG	8 cut(s)	288, 310, 368, 700, 910, 937, 968, 1064
HspAI	GCGC	1 cut(s)	646
Ksp22I	TGATCA	2 cut(s)	823, 967
Kzo9I	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
LmnI	GCTCC	4 cut(s)	343, 643, 772, 878
Lsp1109I	GCAGC	5 cut(s)	366, 787, 830, 915, 1015
LweI	GCATC	4 cut(s)	171, 740, 893, 1099
MaeIII	GTNAC	4 cut(s)	358, 722, 851, 1042
MalI	GATC	5 cut(s)	825, 969, 1052, 1131, 1228
MboI	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
MfeI	CAATTG	1 cut(s)	735
MflI	RGATCY	1 cut(s)	1129
MluCI	AATT	8 cut(s)	82, 301, 327, 735, 767, 954, 1219, 1239
MlyI	GAGTC	2 cut(s)	407, 946
MmeI	TCCRAC	3 cut(s)	150, 273, 292
MseI	TTAA	3 cut(s)	27, 396, 1070
MslI	CAYNNNNRTG	3 cut(s)	638, 678, 911
MspA1I	CMGCKG	3 cut(s)	622, 821, 1102
MspI	CCGG	1 cut(s)	551
MspR9I	CCNGG	2 cut(s)	499, 559
MunI	CAATTG	1 cut(s)	735
Mva1269I	GAATGC	1 cut(s)	171
MvaI	CCWGG	2 cut(s)	499, 559
MwoI	GCNNNNNNNGC	3 cut(s)	654, 870, 1118
NcoI	CCATGG	1 cut(s)	284
NdeII	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
NlaIII	CATG	8 cut(s)	288, 310, 368, 700, 910, 937, 968, 1064
NlaIV	GGNNCC	2 cut(s)	22, 692
NmuCI	GTSAC	4 cut(s)	358, 722, 851, 1042
NspI	RCATGY	1 cut(s)	310
PaeR7I	CTCGAG	1 cut(s)	1199
PagI	TCATGA	2 cut(s)	933, 964
PctI	GAATGC	1 cut(s)	171
PfeI	GAWTC	3 cut(s)	122, 197, 502
PflMI	CCANNNNNTGG	2 cut(s)	370, 923
PkrI	GCNGC	7 cut(s)	381, 656, 659, 777, 820, 905, 1030
PleI	GAGTC	2 cut(s)	407, 945
PpsI	GAGTC	2 cut(s)	407, 945
PpuMI	RGGWCCY	2 cut(s)	20, 690
PshBI	ATTAAT	1 cut(s)	27
Psp5II	RGGWCCY	2 cut(s)	20, 690
Psp6I	CCWGG	2 cut(s)	497, 557
PspEI	GGTNACC	1 cut(s)	722
PspFI	CCCAGC	3 cut(s)	464, 476, 531
PspGI	CCWGG	2 cut(s)	497, 557
PspN4I	GGNNCC	2 cut(s)	22, 692
PspPI	GGNCC	2 cut(s)	20, 690
PspPPI	RGGWCCY	2 cut(s)	20, 690
PstI	CTGCAG	2 cut(s)	905, 1033
PstNI	CAGNNNCTG	1 cut(s)	476
PsuI	RGATCY	1 cut(s)	1129
PvuII	CAGCTG	2 cut(s)	821, 1102
RsaI	GTAC	2 cut(s)	510, 1254
RsaNI	GTAC	2 cut(s)	509, 1253
RseI	CAYNNNNRTG	3 cut(s)	638, 678, 911
SaqAI	TTAA	3 cut(s)	27, 396, 1070
SatI	GCNGC	7 cut(s)	380, 655, 658, 776, 819, 904, 1029
Sau3AI	GATC	5 cut(s)	823, 967, 1050, 1129, 1226
Sau96I	GGNCC	2 cut(s)	20, 690
SchI	GAGTC	2 cut(s)	407, 946
ScrFI	CCNGG	2 cut(s)	499, 559
SfaNI	GCATC	4 cut(s)	171, 740, 893, 1099
SfcI	CTRYAG	2 cut(s)	901, 1029
Sfr274I	CTCGAG	1 cut(s)	1199
SinI	GGWCC	2 cut(s)	20, 690
SlaI	CTCGAG	1 cut(s)	1199
SmiMI	CAYNNNNRTG	3 cut(s)	638, 678, 911
SmlI	CTYRAG	3 cut(s)	100, 347, 1199
SmoI	CTYRAG	3 cut(s)	100, 347, 1199
Sse9I	AATT	8 cut(s)	82, 301, 327, 735, 767, 954, 1219, 1239
SsiI	CCGC	7 cut(s)	574, 622, 638, 652, 655, 658, 984
SspI	AATATT	2 cut(s)	847, 1263
StyD4I	CCNGG	2 cut(s)	497, 557
StyI	CCWWGG	3 cut(s)	284, 448, 832
TaaI	ACNGT	3 cut(s)	1048, 1144, 1180
TaqI	TCGA	3 cut(s)	69, 993, 1200
TasI	AATT	8 cut(s)	82, 301, 327, 735, 767, 954, 1219, 1239
TatI	WGTACW	1 cut(s)	1252
TauI	GCSGC	2 cut(s)	657, 660
TfiI	GAWTC	3 cut(s)	122, 197, 502
Tru1I	TTAA	3 cut(s)	27, 396, 1070
Tru9I	TTAA	3 cut(s)	27, 396, 1070
TscAI	CASTG	4 cut(s)	1012, 1051, 1128, 1183
TseFI	GTSAC	4 cut(s)	358, 722, 851, 1042
TseI	GCWGC	5 cut(s)	379, 775, 818, 903, 1028
Tsp45I	GTSAC	4 cut(s)	358, 722, 851, 1042
TspDTI	ATGAA	6 cut(s)	680, 773, 922, 1014, 1251, 1261
TspGWI	ACGGA	1 cut(s)	54
TspRI	CASTG	4 cut(s)	1012, 1051, 1128, 1183
Van91I	CCANNNNNTGG	2 cut(s)	370, 923
VpaK11BI	GGWCC	2 cut(s)	20, 690
VspI	ATTAAT	1 cut(s)	27
XapI	RAATTY	1 cut(s)	82
XceI	RCATGY	1 cut(s)	310
XcmI	CCANNNNNNNNNTGG	4 cut(s)	495, 953, 1091, 1120
XhoI	CTCGAG	1 cut(s)	1199

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.