AT3G03860

5'-adenylylsulfate reductase-like 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008614
Physical Location & Seq
Forward (+)
992074 .. 994697
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G03860.1

Sequence Viewer

Length: 903 bp
ATGGATTCTCGAGTTTCGATTCTCTTCGTCTGTGCAATAGCCGTGTCATGCTTTACGTCTGGGTCTGCTTCATCACCAGTGGACTTTTCCGTTTGCAATTACGAATTCGAGCTGTTTCGATTCGATTTAGAGGCCAAATGTCCTCCTTCTCTGTATCCAACTCCTCCTATTGAGGTAGATGGAGACTCACTAGACAGGCTAATGGCTTCACAACATGGAAATGCTTATATGTCTGTACTCTTTTATGCTTCCTGGTGCCCATTCTCACGTGCTGTGCGCCCTAAGTTTGATATGTTGAGTTCCATGTTTCCTCAAATACAACACCTAGCTGTGGAGCATTCTCAAGCACTGCCATCTGTCTTTTCTAGGTATGGTATCCATAGCTTACCTTCAATCCTGATGGTAAATCAAACTTTAAATGCTCGGTACCATGGTCGAAAGGATCTTATATCCCTGATTGAGTTTTATGAAGAGGCAACAGGCCTCCAACCTGTCCAGTATGTGGCTGAAGGGGAACCAACAGGCTTAAATGCCGGTGATGGTAACTTGATCACATGGTTGCGCAAGGGGACATCTATTAGAGAAATATTCAAACAAGATCCGTTCTTGGTACTATCTCTGCTGTTTATCTGTTTACAAATGGCAATCCTTGTCTTCCCCATAGCTGAATCCCGCATGAGAGCGTTATGGGCTTCTTACGTTGCCAATTTGAACTTGGGAAGATTTGGAGAGATAAGCCAACTGTTCAACCGTGGTATTCACATGGTCGATGTGAGGAGGTTATGGTTGAAACTAAGTCTCGTCAAAACAAGAAACTTCCATGAAAGGGCAAAGAACGCACAAGCTTGGGCTTCATCGCTGGCATCTGTCTCTCTAGGCCAAACTTCATCAGACCAATCCTGA

Protein Analysis

300

Amino Acids

33.65

Weight (kDa)

7.04

Isoelectric Point (pI)

49.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 74 - 155 3e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 563
Acc65I GGTACC 1 cut(s) 426
AccB1I GGYRCC 2 cut(s) 255, 426
AccB7I CCANNNNNTGG 1 cut(s) 502
AciI CCGC 1 cut(s) 673
AclWI GGATC 2 cut(s) 450, 593
AcsI RAATTY 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 528
AcvI CACGTG 1 cut(s) 269
AfaI GTAC 3 cut(s) 237, 428, 612
AfiI CCNNNNNNNGG 3 cut(s) 331, 502, 826
AgsI TTSAA 5 cut(s) 393, 592, 712, 748, 790
AjnI CCWGG 1 cut(s) 251
AluBI AGCT 5 cut(s) 112, 329, 384, 665, 845
AluI AGCT 5 cut(s) 112, 329, 384, 665, 845
Alw26I GTCTC 3 cut(s) 177, 803, 874
AlwI GGATC 2 cut(s) 450, 593
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 3 cut(s) 132, 481, 877
ApoI RAATTY 1 cut(s) 104
Asp718I GGTACC 1 cut(s) 426
AspLEI GCGC 2 cut(s) 279, 564
AsuHPI GGTGA 2 cut(s) 66, 548
AvaI CYCGRG 1 cut(s) 9
BaeGI GKGCMC 1 cut(s) 260
BaeI ACNNNNGTAYC 2 cut(s) 418, 451
BanI GGYRCC 2 cut(s) 255, 426
BbrPI CACGTG 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 646
BccI CCATC 4 cut(s) 173, 361, 394, 533
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 1 cut(s) 253
BciVI GTATCC 2 cut(s) 165, 386
BclI TGATCA 1 cut(s) 549
BcoDI GTCTC 3 cut(s) 177, 803, 874
BfaI CTAG 4 cut(s) 191, 326, 366, 875
BfuI GTATCC 2 cut(s) 165, 386
Bme1390I CCNGG 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 9
BmiI GGNNCC 3 cut(s) 257, 428, 516
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 872
BpiI GAAGAC 1 cut(s) 646
BpuEI CTTGAG 1 cut(s) 327
BsaAI YACGTR 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 430, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 502, 826
Bse118I RCCGGY 1 cut(s) 533
Bse1I ACTGG 2 cut(s) 77, 496
BseBI CCWGG 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 430, 751
BseLI CCNNNNNNNGG 3 cut(s) 331, 502, 826
BseNI ACTGG 2 cut(s) 77, 496
BseRI GAGGAG 2 cut(s) 153, 790
BseSI GKGCMC 1 cut(s) 260
BshFI GGCC 3 cut(s) 134, 483, 879
BshNI GGYRCC 2 cut(s) 255, 426
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 534
BslFI GGGAC 1 cut(s) 583
BslI CCNNNNNNNGG 3 cut(s) 331, 502, 826
BsmAI GTCTC 3 cut(s) 177, 803, 874
BsmFI GGGAC 1 cut(s) 583
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 3 cut(s) 134, 483, 879
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 260
Bsp143I GATC 3 cut(s) 442, 549, 598
Bsp19I CCATGG 1 cut(s) 430
BspACI CCGC 1 cut(s) 673
BspANI GGCC 3 cut(s) 134, 483, 879
BspLI GGNNCC 3 cut(s) 257, 428, 516
BspPI GGATC 2 cut(s) 450, 593
BspT107I GGYRCC 2 cut(s) 255, 426
BsrFI RCCGGY 1 cut(s) 533
BsrI ACTGG 2 cut(s) 77, 496
BssAI RCCGGY 1 cut(s) 533
BssECI CCNNGG 2 cut(s) 430, 751
BssMI GATC 3 cut(s) 442, 549, 598
BssT1I CCWWGG 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 253
Bst4CI ACNGT 2 cut(s) 744, 752
Bst6I CTCTTC 2 cut(s) 29, 465
BstBAI YACGTR 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 861
BstDEI CTNAG 2 cut(s) 282, 794
BstDSI CCRYGG 2 cut(s) 430, 751
BstHHI GCGC 2 cut(s) 279, 564
BstKTI GATC 3 cut(s) 445, 552, 601
BstMAI GTCTC 3 cut(s) 177, 803, 874
BstMBI GATC 3 cut(s) 442, 549, 598
BstMWI GCNNNNNNNGC 2 cut(s) 689, 836
BstNI CCWGG 1 cut(s) 253
BstSCI CCNGG 1 cut(s) 251
BstSLI GKGCMC 1 cut(s) 260
BstV2I GAAGAC 1 cut(s) 646
BstX2I RGATCY 2 cut(s) 442, 598
BstYI RGATCY 2 cut(s) 442, 598
BsuI GTATCC 2 cut(s) 165, 386
BsuRI GGCC 3 cut(s) 134, 483, 879
BtgI CCRYGG 2 cut(s) 430, 751
BtgZI GCGATG 1 cut(s) 840
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 2 cut(s) 84, 347
Cac8I GCNNGC 1 cut(s) 861
CfoI GCGC 2 cut(s) 279, 564
Cfr10I RCCGGY 1 cut(s) 533
Csp6I GTAC 3 cut(s) 236, 427, 611
CviAII CATG 8 cut(s) 48, 215, 304, 431, 555, 676, 763, 821
CviQI GTAC 3 cut(s) 236, 427, 611
DdeI CTNAG 2 cut(s) 282, 794
DpnI GATC 3 cut(s) 444, 551, 600
DpnII GATC 3 cut(s) 442, 549, 598
DraI TTTAAA 1 cut(s) 417
Eam1104I CTCTTC 2 cut(s) 29, 465
EarI CTCTTC 2 cut(s) 29, 465
Eco130I CCWWGG 1 cut(s) 430
Eco147I AGGCCT 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 528
Eco72I CACGTG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 104
EcoRII CCWGG 1 cut(s) 251
EcoT14I CCWWGG 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 430
FaeI CATG 8 cut(s) 51, 218, 307, 434, 558, 679, 766, 824
FaqI GGGAC 1 cut(s) 583
FatI CATG 8 cut(s) 47, 214, 303, 430, 554, 675, 762, 820
FauI CCCGC 1 cut(s) 680
FbaI TGATCA 1 cut(s) 549
FspBI CTAG 4 cut(s) 191, 326, 366, 875
FspI TGCGCA 1 cut(s) 563
GlaI GCGC 2 cut(s) 278, 563
HaeIII GGCC 3 cut(s) 134, 483, 879
HapII CCGG 1 cut(s) 534
HhaI GCGC 2 cut(s) 279, 564
Hin1II CATG 8 cut(s) 51, 218, 307, 434, 558, 679, 766, 824
Hin6I GCGC 2 cut(s) 277, 562
HinP1I GCGC 2 cut(s) 277, 562
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 5 cut(s) 5, 19, 120, 185, 668
HpaII CCGG 1 cut(s) 534
HphI GGTGA 2 cut(s) 66, 548
Hpy166II GTNNAC 2 cut(s) 82, 635
Hpy188I TCNGA 1 cut(s) 892
Hpy188III TCNNGA 3 cut(s) 9, 397, 900
Hpy8I GTNNAC 2 cut(s) 82, 635
HpyAV CCTTC 3 cut(s) 156, 399, 503
HpyCH4III ACNGT 2 cut(s) 744, 752
HpyCH4IV ACGT 3 cut(s) 56, 268, 699
HpyCH4V TGCA 2 cut(s) 35, 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 689, 836
HpyF3I CTNAG 2 cut(s) 282, 794
HpySE526I ACGT 3 cut(s) 56, 268, 699
Hsp92II CATG 8 cut(s) 51, 218, 307, 434, 558, 679, 766, 824
HspAI GCGC 2 cut(s) 277, 562
KpnI GGTACC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 3 cut(s) 442, 549, 598
LmnI GCTCC 1 cut(s) 334
LweI GCATC 1 cut(s) 872
MaeI CTAG 4 cut(s) 191, 326, 366, 875
MaeII ACGT 3 cut(s) 56, 268, 699
MaeIII GTNAC 1 cut(s) 542
MalI GATC 3 cut(s) 444, 551, 600
MboI GATC 3 cut(s) 442, 549, 598
MboII GAAGA 4 cut(s) 16, 482, 646, 732
MflI RGATCY 2 cut(s) 442, 598
MhlI GDGCHC 1 cut(s) 260
MluCI AATT 3 cut(s) 97, 104, 706
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 2 cut(s) 182, 511
MnlI CCTC 9 cut(s) 124, 153, 166, 174, 321, 466, 494, 768, 771
MseI TTAA 2 cut(s) 416, 527
MslI CAYNNNNRTG 1 cut(s) 219
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 253
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 689, 836
NcoI CCATGG 1 cut(s) 430
NdeII GATC 3 cut(s) 442, 549, 598
NlaIII CATG 8 cut(s) 51, 218, 307, 434, 558, 679, 766, 824
NlaIV GGNNCC 3 cut(s) 257, 428, 516
NsbI TGCGCA 1 cut(s) 563
PaeR7I CTCGAG 1 cut(s) 9
PceI AGGCCT 1 cut(s) 483
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 4 cut(s) 5, 19, 120, 668
PflMI CCANNNNNTGG 1 cut(s) 502
PleI GAGTC 1 cut(s) 179
PmaCI CACGTG 1 cut(s) 269
PmlI CACGTG 1 cut(s) 269
PpsI GAGTC 1 cut(s) 179
Ppu21I YACGTR 1 cut(s) 269
Psp6I CCWGG 1 cut(s) 251
PspCI CACGTG 1 cut(s) 269
PspGI CCWGG 1 cut(s) 251
PspN4I GGNNCC 3 cut(s) 257, 428, 516
PsuI RGATCY 2 cut(s) 442, 598
RsaI GTAC 3 cut(s) 237, 428, 612
RsaNI GTAC 3 cut(s) 236, 427, 611
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 2 cut(s) 416, 527
Sau3AI GATC 3 cut(s) 442, 549, 598
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 1 cut(s) 253
SduI GDGCHC 1 cut(s) 260
SfaNI GCATC 1 cut(s) 872
Sfr274I CTCGAG 1 cut(s) 9
SlaI CTCGAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 9, 342
SmoI CTYRAG 2 cut(s) 9, 342
Sse9I AATT 3 cut(s) 97, 104, 706
SseBI AGGCCT 1 cut(s) 483
SsiI CCGC 1 cut(s) 673
SspI AATATT 1 cut(s) 588
SspMI CTAG 4 cut(s) 191, 326, 366, 875
StuI AGGCCT 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 1 cut(s) 430
TaaI ACNGT 2 cut(s) 744, 752
TaiI ACGT 3 cut(s) 59, 271, 702
TaqI TCGA 7 cut(s) 10, 17, 108, 118, 123, 436, 768
TasI AATT 3 cut(s) 97, 104, 706
TatI WGTACW 1 cut(s) 235
TfiI GAWTC 4 cut(s) 5, 19, 120, 668
Tru1I TTAA 2 cut(s) 416, 527
Tru9I TTAA 2 cut(s) 416, 527
TscAI CASTG 2 cut(s) 84, 354
TspDTI ATGAA 5 cut(s) 60, 483, 837, 843, 876
TspGWI ACGGA 2 cut(s) 79, 591
TspRI CASTG 2 cut(s) 84, 354
Van91I CCANNNNNTGG 1 cut(s) 502
XapI RAATTY 1 cut(s) 104
XcmI CCANNNNNNNNNTGG 1 cut(s) 712
XhoI CTCGAG 1 cut(s) 9
XspI CTAG 4 cut(s) 191, 326, 366, 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.