RchiOBHm_Chr6g0254961

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008614
Physical Location & Seq
Reverse (-)
9893757 .. 9896837
3081 bp
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UTR
Exon/CDS
Intron
PRQ22871

Sequence Viewer

Length: 579 bp
ATGGGTTTTAAATCTGCAATCTTCTTGTTGTTGTTATATATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTATCTGCCCTCCCCAGTCCGATTTGTTCCGTATCACTCTTCAATCTCAATGCCCCGCCGCAACCTCCCCCTACCCACCCCTCCAGGTGGATGGGAACTTCATTGACAGAGCGTTAGCATCAACACGGAACACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGAACATTTAGCAGTTGAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTTTGGTCCAAAAGATCTCCTGTCCCTCGTCCAATTTTACCAGAAAACCACAGTCAATTATTGCAGTGCTAGTTATCACTCTTGGCGTTACAAAGCGAACTCACAACTGGTGAGGATAATATGGACAACAAGCTTGATTCTCTGTTTGTACCTAGAATAG

Protein Analysis

192

Amino Acids

21.88

Weight (kDa)

9.14

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 75 - 153 1.7e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 246
AciI CCGC 2 cut(s) 135, 138
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 569
AfiI CCNNNNNNNGG 2 cut(s) 166, 307
AgsI TTSAA 2 cut(s) 122, 281
AjnI CCWGG 2 cut(s) 162, 242
AluBI AGCT 3 cut(s) 291, 375, 552
AluI AGCT 3 cut(s) 291, 375, 552
Alw21I GWGCWC 1 cut(s) 231
AspS9I GGNCC 2 cut(s) 412, 425
AsuHPI GGTGA 1 cut(s) 541
AvaII GGWCC 2 cut(s) 412, 425
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 164, 244
BciVI GTATCC 1 cut(s) 279
BfaI CTAG 3 cut(s) 372, 489, 572
BfuI GTATCC 1 cut(s) 279
BglII AGATCT 1 cut(s) 433
BisI GCNGC 1 cut(s) 138
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 2 cut(s) 164, 244
Bme18I GGWCC 2 cut(s) 412, 425
BmgT120I GGNCC 2 cut(s) 412, 425
BmiI GGNNCC 2 cut(s) 248, 413
BmrFI CCNGG 2 cut(s) 164, 244
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 288, 340
BsaHI GRCGYC 1 cut(s) 404
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 307
Bse1I ACTGG 3 cut(s) 94, 217, 531
BseBI CCWGG 2 cut(s) 164, 244
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 166, 307
BseNI ACTGG 3 cut(s) 94, 217, 531
BseSI GKGCMC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 231
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 166, 307
BsmFI GGGAC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 231, 251
Bsp143I GATC 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 2 cut(s) 135, 138
BspLI GGNNCC 2 cut(s) 248, 413
BspT107I GGYRCC 1 cut(s) 246
BsrI ACTGG 3 cut(s) 94, 217, 531
BssMI GATC 1 cut(s) 433
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 164, 244
Bst4CI ACNGT 1 cut(s) 472
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 404
BstDEI CTNAG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 1 cut(s) 436
BstMBI GATC 1 cut(s) 433
BstNI CCWGG 2 cut(s) 164, 244
BstSCI CCNGG 2 cut(s) 162, 242
BstSLI GKGCMC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 175
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 2 cut(s) 210, 490
Cfr13I GGNCC 2 cut(s) 412, 425
Csp6I GTAC 1 cut(s) 568
CviAII CATG 1 cut(s) 295
CviJI RGCY 4 cut(s) 291, 338, 375, 552
CviKI_1 RGCY 4 cut(s) 291, 338, 375, 552
CviQI GTAC 1 cut(s) 568
DdeI CTNAG 1 cut(s) 287
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 412, 425
Eco57I CTGAAG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 162, 242
FaeI CATG 1 cut(s) 298
FaiI YATR 6 cut(s) 37, 39, 219, 296, 363, 541
FaqI GGGAC 1 cut(s) 427
FatI CATG 1 cut(s) 294
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 3 cut(s) 372, 489, 572
GluI GCNGC 1 cut(s) 138
GsuI CTGGAG 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 2 cut(s) 366, 556
HphI GGTGA 1 cut(s) 541
Hpy188I TCNGA 3 cut(s) 100, 402, 416
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 349, 390
HpyCH4III ACNGT 1 cut(s) 472
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 17, 483
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 1 cut(s) 298
Kzo9I GATC 1 cut(s) 433
LpnPI CCDG 9 cut(s) 107, 149, 176, 198, 229, 256, 452, 473, 512
LweI GCATC 1 cut(s) 206
MaeI CTAG 3 cut(s) 372, 489, 572
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 506
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 4 cut(s) 13, 66, 110, 324
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 231, 251
MluCI AATT 4 cut(s) 59, 263, 452, 475
MnlI CCTC 6 cut(s) 99, 154, 170, 312, 455, 525
MseI TTAA 2 cut(s) 9, 389
MspR9I CCNGG 2 cut(s) 164, 244
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 2 cut(s) 164, 244
NdeII GATC 1 cut(s) 433
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 2 cut(s) 248, 413
PfeI GAWTC 2 cut(s) 366, 556
PkrI GCNGC 1 cut(s) 139
Psp6I CCWGG 2 cut(s) 162, 242
PspGI CCWGG 2 cut(s) 162, 242
PspN4I GGNNCC 2 cut(s) 248, 413
PspPI GGNCC 2 cut(s) 412, 425
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 569
RsaNI GTAC 1 cut(s) 568
SaqAI TTAA 2 cut(s) 9, 389
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 2 cut(s) 412, 425
ScrFI CCNGG 2 cut(s) 164, 244
SduI GDGCHC 2 cut(s) 231, 251
SetI ASST 7 cut(s) 146, 168, 293, 377, 407, 554, 573
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 2 cut(s) 412, 425
SmlI CTYRAG 2 cut(s) 303, 355
SmoI CTYRAG 2 cut(s) 303, 355
Sse9I AATT 4 cut(s) 59, 263, 452, 475
SsiI CCGC 2 cut(s) 135, 138
SspMI CTAG 3 cut(s) 372, 489, 572
StyD4I CCNGG 2 cut(s) 162, 242
TaaI ACNGT 1 cut(s) 472
TaiI ACGT 1 cut(s) 407
TasI AATT 4 cut(s) 59, 263, 452, 475
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 2 cut(s) 366, 556
Tru1I TTAA 2 cut(s) 9, 389
Tru9I TTAA 2 cut(s) 9, 389
TscAI CASTG 2 cut(s) 217, 490
TspDTI ATGAA 2 cut(s) 169, 283
TspGWI ACGGA 2 cut(s) 98, 220
TspRI CASTG 2 cut(s) 217, 490
VpaK11BI GGWCC 2 cut(s) 412, 425
XspI CTAG 3 cut(s) 372, 489, 572
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.