MD14G1003500.v1.1

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008614
Physical Location & Seq
Forward (+)
419965 .. 423987
4023 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1003500.v1.1.491

Sequence Viewer

Length: 843 bp
ATGGATAAATCTGTAATCTTTTTGTATATCGCTGCCTTGACAACTTCTCTAGGATTAGTTTCTTCTTCTTCTGTTCTATGCCCTCACGACTCCGATTTCTTCCGATACGGCCTTCAATCTCAATGCCCTATCTCGATCTCCCCCCACCCACCCCTCAAGGTGGATGGAAACTTCCTAGACAGAGCTTTGGCATCGCAACAGAGGACTGATTATGCAGCAGTGCTCTTTTATGCTTCCTGGTGCCCATTTTCACGCATAATGTATCCTACATTTGAAAAGCTCAGCTTCATGTTCCCTCAAGTACAACATTTGGCCATTGAGCAATCTTCAGCCCTGCCAAGTGTGTTCTCAAGATATGGAATACATAGCTTCCCTTCGATTTTAATAGTCAATCAGACATCAAGGGTGCGTTATCATGGTCCAAAAAGTCTCTCCTTGCTTGCACAGTTTTACCAGAAAACCACAGGACTTAAACCAGTTCAATATTTTGATGGCGATCAAATAGTCAGCTTAAACATTCGTGAAAAATCTTTAATCCAGTCAATGAGTAACATGTCACTCAGGGAGATATCAAGGAGGGAACCCTACTTAGCTTTTGCCATATTGTTTCTCTGTTTGAGGGTGATTCTGTATACATTCCCAAAGGTATTGACTCGCCTCCAAGCTTTCTGGGTCCTATACGTTCCCCATTTTAACTTGGGAATATTTGGGGAGACGAGTCAATTCCACGAGGGAGCAAAGAACGCCAGGGTTTGGGCTTCTTCCCTAACTTCTGTCTCCTTGGGCGAATCTTCATCTGCTCGATCATCGTCATTGTCATGTCATCTTAAATGCAGTACTTAA

Protein Analysis

281

Amino Acids

31.54

Weight (kDa)

9.33

Isoelectric Point (pI)

55.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 69 - 151 8.5e-08 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 240
AccB7I CCANNNNNTGG 1 cut(s) 753
AccI GTMKAC 1 cut(s) 632
AcoI YGGCCR 1 cut(s) 312
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 2 cut(s) 303, 838
AfiI CCNNNNNNNGG 2 cut(s) 160, 753
AflIII ACRYGT 1 cut(s) 552
AgsI TTSAA 3 cut(s) 116, 275, 482
AjnI CCWGG 2 cut(s) 236, 746
AluBI AGCT 7 cut(s) 185, 280, 285, 369, 510, 593, 665
AluI AGCT 7 cut(s) 185, 280, 285, 369, 510, 593, 665
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 434, 707, 781
AoxI GGCC 2 cut(s) 109, 312
ApeKI GCWGC 2 cut(s) 32, 215
AspS9I GGNCC 2 cut(s) 419, 673
AsuHPI GGTGA 1 cut(s) 634
AvaII GGWCC 2 cut(s) 419, 673
BaeGI GKGCMC 1 cut(s) 245
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 240
BauI CACGAG 1 cut(s) 728
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 19, 227
BccI CCATC 2 cut(s) 158, 485
BceAI ACGGC 1 cut(s) 124
BciT130I CCWGG 2 cut(s) 238, 748
BciVI GTATCC 1 cut(s) 273
BcoDI GTCTC 3 cut(s) 434, 707, 781
BfaI CTAG 2 cut(s) 50, 176
BfuI GTATCC 1 cut(s) 273
BisI GCNGC 2 cut(s) 33, 216
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 2 cut(s) 34, 217
BmcAI AGTACT 1 cut(s) 838
Bme1390I CCNGG 2 cut(s) 238, 748
Bme18I GGWCC 2 cut(s) 419, 673
BmgT120I GGNCC 2 cut(s) 419, 673
BmiI GGNNCC 3 cut(s) 242, 582, 674
BmrFI CCNGG 2 cut(s) 238, 748
BmsI GCATC 1 cut(s) 200
Bpu1102I GCTNAGC 1 cut(s) 281
BpuEI CTTGAG 3 cut(s) 140, 282, 334
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 747, 780
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 753
Bse1I ACTGG 2 cut(s) 476, 538
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 2 cut(s) 238, 748
BseDI CCNNGG 2 cut(s) 747, 780
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 2 cut(s) 160, 753
BseMII CTCAG 2 cut(s) 295, 574
BseNI ACTGG 2 cut(s) 476, 538
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 2 cut(s) 19, 227
BshFI GGCC 2 cut(s) 111, 314
BshNI GGYRCC 1 cut(s) 240
BsiHKAI GWGCWC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 160, 753
BsmAI GTCTC 3 cut(s) 434, 707, 781
BsmBI CGTCTC 1 cut(s) 707
BsnI GGCC 2 cut(s) 111, 314
Bsp1286I GDGCHC 2 cut(s) 225, 245
Bsp143I GATC 3 cut(s) 135, 496, 803
Bsp1720I GCTNAGC 1 cut(s) 281
BspANI GGCC 2 cut(s) 111, 314
BspCNI CTCAG 2 cut(s) 294, 573
BspLI GGNNCC 3 cut(s) 242, 582, 674
BspT107I GGYRCC 1 cut(s) 240
BsrI ACTGG 2 cut(s) 476, 538
BssECI CCNNGG 2 cut(s) 747, 780
BssMI GATC 3 cut(s) 135, 496, 803
BssNAI GTATAC 1 cut(s) 633
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 1 cut(s) 780
Bst1107I GTATAC 1 cut(s) 633
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 2 cut(s) 238, 748
Bst4CI ACNGT 1 cut(s) 447
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 281, 560, 589
BstF5I GGATG 1 cut(s) 169
BstKTI GATC 3 cut(s) 138, 499, 806
BstMAI GTCTC 3 cut(s) 434, 707, 781
BstMBI GATC 3 cut(s) 135, 496, 803
BstMWI GCNNNNNNNGC 1 cut(s) 743
BstNI CCWGG 2 cut(s) 238, 748
BstNSI RCATGY 1 cut(s) 556
BstSCI CCNGG 2 cut(s) 236, 746
BstSLI GKGCMC 1 cut(s) 245
BstV1I GCAGC 2 cut(s) 19, 227
BstZ17I GTATAC 1 cut(s) 633
BsuI GTATCC 1 cut(s) 273
BsuRI GGCC 2 cut(s) 111, 314
BtgZI GCGATG 1 cut(s) 177
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 1 cut(s) 441
Cfr13I GGNCC 2 cut(s) 419, 673
Csp6I GTAC 2 cut(s) 302, 837
CviAII CATG 4 cut(s) 289, 416, 553, 819
CviQI GTAC 2 cut(s) 302, 837
DdeI CTNAG 3 cut(s) 281, 560, 589
DpnI GATC 3 cut(s) 137, 498, 805
DpnII GATC 3 cut(s) 135, 496, 803
EaeI YGGCCR 1 cut(s) 312
Eco130I CCWWGG 1 cut(s) 780
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 419, 673
Eco57I CTGAAG 1 cut(s) 312
EcoO109I RGGNCCY 1 cut(s) 673
EcoRII CCWGG 2 cut(s) 236, 746
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 780
ErhI CCWWGG 1 cut(s) 780
Esp3I CGTCTC 1 cut(s) 707
FaeI CATG 4 cut(s) 292, 419, 556, 822
FalI AAGNNNNNCTT 2 cut(s) 269, 301
FatI CATG 4 cut(s) 288, 415, 552, 818
FblI GTMKAC 1 cut(s) 632
Fnu4HI GCNGC 2 cut(s) 33, 216
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 33, 216
FspBI CTAG 2 cut(s) 50, 176
GluI GCNGC 2 cut(s) 33, 216
HaeIII GGCC 2 cut(s) 111, 314
Hin1II CATG 4 cut(s) 292, 419, 556, 822
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 5 cut(s) 89, 625, 652, 718, 788
HphI GGTGA 1 cut(s) 634
Hpy166II GTNNAC 1 cut(s) 633
Hpy188I TCNGA 3 cut(s) 94, 104, 396
Hpy188III TCNNGA 4 cut(s) 86, 133, 351, 521
Hpy8I GTNNAC 1 cut(s) 633
HpyAV CCTTC 2 cut(s) 122, 384
HpyCH4III ACNGT 1 cut(s) 447
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 3 cut(s) 215, 443, 834
HpyF10VI GCNNNNNNNGC 1 cut(s) 743
HpyF3I CTNAG 3 cut(s) 281, 560, 589
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 4 cut(s) 292, 419, 556, 822
Kzo9I GATC 3 cut(s) 135, 496, 803
LmnI GCTCC 1 cut(s) 734
Lsp1109I GCAGC 2 cut(s) 19, 227
LweI GCATC 1 cut(s) 200
MaeI CTAG 2 cut(s) 50, 176
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 2 cut(s) 548, 555
MalI GATC 3 cut(s) 137, 498, 805
MboI GATC 3 cut(s) 135, 496, 803
MboII GAAGA 7 cut(s) 54, 57, 60, 91, 318, 753, 783
MhlI GDGCHC 2 cut(s) 225, 245
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 1 cut(s) 722
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 3 cut(s) 83, 646, 727
MnlI CCTC 8 cut(s) 93, 164, 195, 306, 570, 612, 668, 724
Mox20I TGGCCA 1 cut(s) 314
MscI TGGCCA 1 cut(s) 314
MseI TTAA 7 cut(s) 383, 471, 512, 533, 693, 828, 841
MslI CAYNNNNRTG 1 cut(s) 817
Msp20I TGGCCA 1 cut(s) 314
MspR9I CCNGG 2 cut(s) 238, 748
MvaI CCWGG 2 cut(s) 238, 748
MwoI GCNNNNNNNGC 1 cut(s) 743
NdeII GATC 3 cut(s) 135, 496, 803
NlaIII CATG 4 cut(s) 292, 419, 556, 822
NlaIV GGNNCC 3 cut(s) 242, 582, 674
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 1 cut(s) 556
PciI ACATGT 1 cut(s) 552
PfeI GAWTC 2 cut(s) 625, 788
PflMI CCANNNNNTGG 1 cut(s) 753
PkrI GCNGC 2 cut(s) 34, 217
PleI GAGTC 3 cut(s) 83, 646, 726
PpsI GAGTC 3 cut(s) 83, 646, 726
PpuMI RGGWCCY 1 cut(s) 673
PscI ACATGT 1 cut(s) 552
Psp5II RGGWCCY 1 cut(s) 673
Psp6I CCWGG 2 cut(s) 236, 746
PspGI CCWGG 2 cut(s) 236, 746
PspN4I GGNNCC 3 cut(s) 242, 582, 674
PspPI GGNCC 2 cut(s) 419, 673
PspPPI RGGWCCY 1 cut(s) 673
RsaI GTAC 2 cut(s) 303, 838
RsaNI GTAC 2 cut(s) 302, 837
RseI CAYNNNNRTG 1 cut(s) 817
SaqAI TTAA 7 cut(s) 383, 471, 512, 533, 693, 828, 841
SatI GCNGC 2 cut(s) 33, 216
Sau3AI GATC 3 cut(s) 135, 496, 803
Sau96I GGNCC 2 cut(s) 419, 673
ScaI AGTACT 1 cut(s) 838
SchI GAGTC 3 cut(s) 83, 646, 727
ScrFI CCNGG 2 cut(s) 238, 748
SduI GDGCHC 2 cut(s) 225, 245
SfaNI GCATC 1 cut(s) 200
SinI GGWCC 2 cut(s) 419, 673
SmiMI CAYNNNNRTG 1 cut(s) 817
SmlI CTYRAG 3 cut(s) 155, 297, 349
SmoI CTYRAG 3 cut(s) 155, 297, 349
Sse9I AATT 1 cut(s) 722
SspI AATATT 2 cut(s) 485, 705
SspMI CTAG 2 cut(s) 50, 176
StyD4I CCNGG 2 cut(s) 236, 746
StyI CCWWGG 1 cut(s) 780
TaaI ACNGT 1 cut(s) 447
TaiI ACGT 1 cut(s) 684
TaqI TCGA 3 cut(s) 134, 377, 802
TasI AATT 1 cut(s) 722
TatI WGTACW 2 cut(s) 301, 836
TfiI GAWTC 2 cut(s) 625, 788
Tru1I TTAA 7 cut(s) 383, 471, 512, 533, 693, 828, 841
Tru9I TTAA 7 cut(s) 383, 471, 512, 533, 693, 828, 841
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 2 cut(s) 32, 215
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 2 cut(s) 277, 783
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 753
VpaK11BI GGWCC 2 cut(s) 419, 673
XceI RCATGY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 632
XspI CTAG 2 cut(s) 50, 176
ZrmI AGTACT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.