Rh3BG369100

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0008614
Physical Location & Seq
Forward (+)
46214390 .. 46219572
5183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG369100.1

Sequence Viewer

Length: 903 bp
ATGGGTTTTAAATCTGCAATCTTGTTGTTGTTGTTATATATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTGTCTGCCCTCCCCAGTCTGATTTGTTCCGTATCACTCTTCAATCTCAATGCCCCGTCGCAGCCTCCCCCTACCCACCCCTCCAGGTGGATGGGAACTTCATTGACAGAGCGTTTGCATCAGCACGGAAGACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGAGCATTTAGCAGTTCAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTTTGGTCCAAAAGATCTCCTGTCCCTTGTCCAATTTTACCAGAAAACCACGGGACTTGAGCCGGTTCAGTATTACAATCATGATCATTCCGTCGGATGTGAATCTGGTGAAAAATCCATAATCAAATCAATGAGTAACCTGCTGTCACTAAGAGAGATATCAAGGAGGGAACCCTATTTAGTTTTCGCTACATTGTTTCTCTGTCTTAGGGTACTTATACATATATTGCCTAAGGTATTGACTCACCTGAAAGCTTTCTGGGTTCTATACGTTCCCCATTTCAACGTGGGAATATTTGGGGAGACGAGTCAAATTATGGGGCGTATCCTTCACATGGTTGATGTTAGGAGGATTTGGAACAAGCTGAGATTATGCAAGACCAGGAATTTCCACGAAGGTGCAAAGAATGCCAGGGTTTGGGCTTCTTCCCTGACTTCAGTCTCTTTGGGTGAATCTTCATCGGCTAGATCATTGTCATCTTAA

Protein Analysis

300

Amino Acids

33.95

Weight (kDa)

9.69

Isoelectric Point (pI)

53.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 76 - 152 6.7e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 246
AccB7I CCANNNNNTGG 1 cut(s) 837
AcsI RAATTY 1 cut(s) 805
AcuI CTGAAG 2 cut(s) 318, 840
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 633
AfiI CCNNNNNNNGG 4 cut(s) 166, 307, 754, 837
AgsI TTSAA 3 cut(s) 122, 281, 703
AjnI CCWGG 4 cut(s) 162, 242, 800, 830
AleI CACNNNNGTG 1 cut(s) 816
AluBI AGCT 4 cut(s) 291, 375, 674, 784
AluI AGCT 4 cut(s) 291, 375, 674, 784
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 716, 865
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 1 cut(s) 805
ArsI GACNNNNNNTTYG 2 cut(s) 176, 208
Asp700I GAANNNNTTC 1 cut(s) 674
AspS9I GGNCC 2 cut(s) 412, 425
AsuHPI GGTGA 3 cut(s) 539, 656, 881
AvaII GGWCC 2 cut(s) 412, 425
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 246
BbsI GAAGAC 2 cut(s) 66, 215
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 152
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 4 cut(s) 164, 244, 802, 832
BciVI GTATCC 2 cut(s) 279, 755
BclI TGATCA 1 cut(s) 502
BcoDI GTCTC 2 cut(s) 716, 865
BfaI CTAG 2 cut(s) 372, 885
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 2 cut(s) 279, 755
BglII AGATCT 1 cut(s) 433
BisI GCNGC 1 cut(s) 141
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 4 cut(s) 164, 244, 802, 832
Bme18I GGWCC 2 cut(s) 412, 425
BmgT120I GGNCC 2 cut(s) 412, 425
BmiI GGNNCC 3 cut(s) 248, 413, 591
BmrFI CCNGG 4 cut(s) 164, 244, 802, 832
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 88
BoxI GACNNNNGTC 1 cut(s) 857
BpiI GAAGAC 2 cut(s) 66, 215
BpmI CTGGAG 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 3 cut(s) 288, 340, 497
BsaBI GATNNNNATC 1 cut(s) 520
BsaHI GRCGYC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 468, 831
Bsc4I CCNNNNNNNGG 4 cut(s) 166, 307, 754, 837
Bse118I RCCGGY 1 cut(s) 481
Bse1I ACTGG 2 cut(s) 94, 217
Bse21I CCTNAGG 1 cut(s) 651
Bse8I GATNNNNATC 1 cut(s) 520
BseBI CCWGG 4 cut(s) 164, 244, 802, 832
BseDI CCNNGG 2 cut(s) 468, 831
BseGI GGATG 2 cut(s) 175, 521
BseJI GATNNNNATC 1 cut(s) 520
BseLI CCNNNNNNNGG 4 cut(s) 166, 307, 754, 837
BseMII CTCAG 1 cut(s) 776
BseNI ACTGG 2 cut(s) 94, 217
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 152
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 482
BslFI GGGAC 2 cut(s) 427, 486
BslI CCNNNNNNNGG 4 cut(s) 166, 307, 754, 837
BsmAI GTCTC 2 cut(s) 716, 865
BsmBI CGTCTC 1 cut(s) 716
BsmFI GGGAC 2 cut(s) 427, 486
BsmI GAATGC 1 cut(s) 832
Bsp1286I GDGCHC 2 cut(s) 231, 251
Bsp143I GATC 3 cut(s) 433, 502, 887
Bsp1720I GCTNAGC 1 cut(s) 287
BspCNI CTCAG 1 cut(s) 777
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 3 cut(s) 248, 413, 591
BspMI ACCTGC 1 cut(s) 567
BspT107I GGYRCC 1 cut(s) 246
BsrFI RCCGGY 1 cut(s) 481
BsrI ACTGG 2 cut(s) 94, 217
BssAI RCCGGY 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 468, 831
BssMI GATC 3 cut(s) 433, 502, 887
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 4 cut(s) 164, 244, 802, 832
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 404
BstAPI GCANNNNNTGC 1 cut(s) 827
BstDEI CTNAG 5 cut(s) 287, 569, 626, 651, 785
BstDSI CCRYGG 1 cut(s) 468
BstF5I GGATG 2 cut(s) 175, 521
BstKTI GATC 3 cut(s) 436, 505, 890
BstMAI GTCTC 2 cut(s) 716, 865
BstMBI GATC 3 cut(s) 433, 502, 887
BstMWI GCNNNNNNNGC 1 cut(s) 827
BstNI CCWGG 4 cut(s) 164, 244, 802, 832
BstPAI GACNNNNGTC 1 cut(s) 857
BstSCI CCNGG 4 cut(s) 162, 242, 800, 830
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 152
BstV2I GAAGAC 2 cut(s) 66, 215
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 433
Bsu36I CCTNAGG 1 cut(s) 651
BsuI GTATCC 2 cut(s) 279, 755
BtgI CCRYGG 1 cut(s) 468
BtsCI GGATG 2 cut(s) 175, 521
BveI ACCTGC 1 cut(s) 567
CciI TCATGA 1 cut(s) 499
Cfr10I RCCGGY 1 cut(s) 481
Cfr13I GGNCC 2 cut(s) 412, 425
Csp6I GTAC 1 cut(s) 632
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 3 cut(s) 295, 500, 754
CviJI RGCY 9 cut(s) 143, 291, 338, 375, 481, 674, 784, 842, 884
CviKI_1 RGCY 9 cut(s) 143, 291, 338, 375, 481, 674, 784, 842, 884
CviQI GTAC 1 cut(s) 632
DdeI CTNAG 5 cut(s) 287, 569, 626, 651, 785
DpnI GATC 3 cut(s) 435, 504, 889
DpnII GATC 3 cut(s) 433, 502, 887
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco32I GATATC 1 cut(s) 579
Eco47I GGWCC 2 cut(s) 412, 425
Eco57I CTGAAG 2 cut(s) 318, 840
Eco81I CCTNAGG 1 cut(s) 651
EcoRII CCWGG 4 cut(s) 162, 242, 800, 830
EcoRV GATATC 1 cut(s) 579
Esp3I CGTCTC 1 cut(s) 716
FaeI CATG 3 cut(s) 298, 503, 757
FaqI GGGAC 2 cut(s) 427, 486
FatI CATG 3 cut(s) 294, 499, 753
FbaI TGATCA 1 cut(s) 502
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 2 cut(s) 182, 528
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 2 cut(s) 372, 885
GluI GCNGC 1 cut(s) 141
GsuI CTGGAG 1 cut(s) 146
HapII CCGG 1 cut(s) 482
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 3 cut(s) 298, 503, 757
HindIII AAGCTT 1 cut(s) 672
HinfI GANTC 5 cut(s) 366, 521, 661, 727, 872
HpaII CCGG 1 cut(s) 482
HphI GGTGA 3 cut(s) 539, 656, 881
Hpy188I TCNGA 4 cut(s) 100, 402, 416, 515
Hpy188III TCNNGA 2 cut(s) 357, 500
Hpy99I CGWCG 3 cut(s) 73, 140, 515
HpyAV CCTTC 4 cut(s) 349, 390, 758, 809
HpyCH4IV ACGT 3 cut(s) 404, 690, 705
HpyCH4V TGCA 4 cut(s) 17, 197, 795, 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 827
HpyF3I CTNAG 5 cut(s) 287, 569, 626, 651, 785
HpySE526I ACGT 3 cut(s) 404, 690, 705
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 3 cut(s) 298, 503, 757
Ksp22I TGATCA 1 cut(s) 502
Kzo9I GATC 3 cut(s) 433, 502, 887
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 152
LweI GCATC 1 cut(s) 206
MaeI CTAG 2 cut(s) 372, 885
MaeII ACGT 3 cut(s) 404, 690, 705
MaeIII GTNAC 2 cut(s) 554, 564
MalI GATC 3 cut(s) 435, 504, 889
MboI GATC 3 cut(s) 433, 502, 887
MboII GAAGA 6 cut(s) 66, 110, 220, 324, 837, 867
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 231, 251
MluCI AATT 5 cut(s) 59, 263, 452, 732, 805
MlyI GAGTC 2 cut(s) 655, 736
MmeI TCCRAC 1 cut(s) 493
MnlI CCTC 6 cut(s) 99, 154, 170, 312, 579, 762
MroXI GAANNNNTTC 1 cut(s) 674
MseI TTAA 3 cut(s) 9, 389, 901
MslI CAYNNNNRTG 1 cut(s) 816
MspI CCGG 1 cut(s) 482
MspR9I CCNGG 4 cut(s) 164, 244, 802, 832
MunI CAATTG 1 cut(s) 59
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 4 cut(s) 164, 244, 802, 832
MwoI GCNNNNNNNGC 1 cut(s) 827
NdeII GATC 3 cut(s) 433, 502, 887
NlaIII CATG 3 cut(s) 298, 503, 757
NlaIV GGNNCC 3 cut(s) 248, 413, 591
NmuCI GTSAC 1 cut(s) 564
OliI CACNNNNGTG 1 cut(s) 816
PagI TCATGA 1 cut(s) 499
PctI GAATGC 1 cut(s) 832
PdmI GAANNNNTTC 1 cut(s) 674
PfeI GAWTC 3 cut(s) 366, 521, 872
PflMI CCANNNNNTGG 1 cut(s) 837
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 2 cut(s) 655, 735
PpsI GAGTC 2 cut(s) 655, 735
PshAI GACNNNNGTC 1 cut(s) 857
Psp6I CCWGG 4 cut(s) 162, 242, 800, 830
PspGI CCWGG 4 cut(s) 162, 242, 800, 830
PspN4I GGNNCC 3 cut(s) 248, 413, 591
PspPI GGNCC 2 cut(s) 412, 425
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 633
RsaNI GTAC 1 cut(s) 632
RseI CAYNNNNRTG 1 cut(s) 816
SaqAI TTAA 3 cut(s) 9, 389, 901
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 3 cut(s) 433, 502, 887
Sau96I GGNCC 2 cut(s) 412, 425
SchI GAGTC 2 cut(s) 655, 736
ScrFI CCNGG 4 cut(s) 164, 244, 802, 832
SduI GDGCHC 2 cut(s) 231, 251
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 2 cut(s) 412, 425
SmiMI CAYNNNNRTG 1 cut(s) 816
SmlI CTYRAG 3 cut(s) 303, 355, 476
SmoI CTYRAG 3 cut(s) 303, 355, 476
Sse9I AATT 5 cut(s) 59, 263, 452, 732, 805
SspI AATATT 1 cut(s) 714
SspMI CTAG 2 cut(s) 372, 885
StyD4I CCNGG 4 cut(s) 162, 242, 800, 830
TaiI ACGT 3 cut(s) 407, 693, 708
TasI AATT 5 cut(s) 59, 263, 452, 732, 805
TfiI GAWTC 3 cut(s) 366, 521, 872
Tru1I TTAA 3 cut(s) 9, 389, 901
Tru9I TTAA 3 cut(s) 9, 389, 901
TseFI GTSAC 1 cut(s) 564
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 3 cut(s) 169, 283, 867
TspGWI ACGGA 3 cut(s) 98, 220, 499
Van91I CCANNNNNTGG 1 cut(s) 837
VpaK11BI GGWCC 2 cut(s) 412, 425
XapI RAATTY 1 cut(s) 805
XmnI GAANNNNTTC 1 cut(s) 674
XspI CTAG 2 cut(s) 372, 885
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.