Rw5G011110

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008614
Physical Location & Seq
Reverse (-)
12456930 .. 12459648
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G011110.1

Sequence Viewer

Length: 636 bp
ATGGGTTTTAAATCTGCAATCTTCTTGTTGTTGTTATATATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTGTCTGCCCTCCCCAGTCCGATTTGTTCCGTATCACTCTTCAATCTCAATGCCCCGCCGCAACCTCCCCCTACCCACCCCTCCAGGTGGATGGGAACTTCATTGACAGAGCGTTAGCATCAACACGGAACACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCACGTTCCCTCAAGTGGAGCATTTAGCAGTTGAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTTTGGTCCAAAAGATCTCCTGTCCCTCGTCCAATTTTACCAGAAAACCACAGCCTTGTTAGTCGTAAGGGTAATTCTAGGTGCATGTCTGAGGTTTCCTGTTCTCCTCATGTCTGTTTGTGAAAGTAGAAAATGCGGTTGGAGGCATGCAGCCCGTTTTTCTTTAGCTTATCCTGTATTCATCCCTTATCCAGTACTTAAATGTTTTCGTTTGTGA

Protein Analysis

211

Amino Acids

23.82

Weight (kDa)

9.58

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 75 - 153 7.5e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
AccB1I GGYRCC 1 cut(s) 246
AciI CCGC 3 cut(s) 135, 138, 555
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 615
AfiI CCNNNNNNNGG 2 cut(s) 166, 307
AgsI TTSAA 2 cut(s) 122, 281
AjnI CCWGG 2 cut(s) 162, 242
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
AluBI AGCT 3 cut(s) 291, 375, 587
AluI AGCT 3 cut(s) 291, 375, 587
Alw21I GWGCWC 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 569
AspS9I GGNCC 2 cut(s) 412, 425
AvaII GGWCC 2 cut(s) 412, 425
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 581
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 164, 244
BciVI GTATCC 1 cut(s) 279
BfaI CTAG 2 cut(s) 372, 497
BfuI GTATCC 1 cut(s) 279
BglII AGATCT 1 cut(s) 433
BisI GCNGC 2 cut(s) 138, 570
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 2 cut(s) 139, 571
BmcAI AGTACT 1 cut(s) 615
Bme1390I CCNGG 2 cut(s) 164, 244
Bme18I GGWCC 2 cut(s) 412, 425
BmgT120I GGNCC 2 cut(s) 412, 425
BmiI GGNNCC 2 cut(s) 248, 413
BmrFI CCNGG 2 cut(s) 164, 244
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 1 cut(s) 206
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 288, 340
BsaHI GRCGYC 1 cut(s) 404
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 307
Bse1I ACTGG 3 cut(s) 94, 217, 611
BseBI CCWGG 2 cut(s) 164, 244
BseGI GGATG 2 cut(s) 175, 600
BseLI CCNNNNNNNGG 2 cut(s) 166, 307
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 3 cut(s) 94, 217, 611
BseRI GAGGAG 1 cut(s) 515
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 581
BshNI GGYRCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 231
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 166, 307
BsmFI GGGAC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 231, 251
Bsp143I GATC 1 cut(s) 433
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 3 cut(s) 135, 138, 555
BspCNI CTCAG 1 cut(s) 501
BspLI GGNNCC 2 cut(s) 248, 413
BspT107I GGYRCC 1 cut(s) 246
BsrI ACTGG 3 cut(s) 94, 217, 611
BssMI GATC 1 cut(s) 433
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 164, 244
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 2 cut(s) 287, 509
BstF5I GGATG 2 cut(s) 175, 600
BstKTI GATC 1 cut(s) 436
BstMBI GATC 1 cut(s) 433
BstNI CCWGG 2 cut(s) 164, 244
BstNSI RCATGY 2 cut(s) 507, 569
BstSCI CCNGG 2 cut(s) 162, 242
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 581
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 1 cut(s) 279
BtsCI GGATG 2 cut(s) 175, 600
BtsIMutI CAGTG 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 567
Cfr13I GGNCC 2 cut(s) 412, 425
Csp6I GTAC 1 cut(s) 614
CviAII CATG 3 cut(s) 504, 529, 566
CviJI RGCY 6 cut(s) 291, 338, 375, 473, 572, 587
CviKI_1 RGCY 6 cut(s) 291, 338, 375, 473, 572, 587
CviQI GTAC 1 cut(s) 614
DdeI CTNAG 2 cut(s) 287, 509
DpnI GATC 1 cut(s) 435
DpnII GATC 1 cut(s) 433
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 412, 425
Eco57I CTGAAG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 162, 242
FaeI CATG 3 cut(s) 507, 532, 569
FaiI YATR 7 cut(s) 37, 39, 219, 363, 505, 530, 567
FaqI GGGAC 1 cut(s) 427
FatI CATG 3 cut(s) 503, 528, 565
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 138, 570
FokI GGATG 2 cut(s) 182, 587
Fsp4HI GCNGC 2 cut(s) 138, 570
FspBI CTAG 2 cut(s) 372, 497
GluI GCNGC 2 cut(s) 138, 570
GsuI CTGGAG 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 3 cut(s) 507, 532, 569
HinfI GANTC 1 cut(s) 366
Hpy188I TCNGA 4 cut(s) 100, 402, 416, 510
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 2 cut(s) 349, 390
HpyCH4IV ACGT 2 cut(s) 296, 404
HpyCH4V TGCA 3 cut(s) 17, 503, 569
HpyF3I CTNAG 2 cut(s) 287, 509
HpySE526I ACGT 2 cut(s) 296, 404
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 3 cut(s) 507, 532, 569
Kzo9I GATC 1 cut(s) 433
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 581
LweI GCATC 1 cut(s) 206
MaeI CTAG 2 cut(s) 372, 497
MaeII ACGT 2 cut(s) 296, 404
MalI GATC 1 cut(s) 435
MboI GATC 1 cut(s) 433
MboII GAAGA 4 cut(s) 13, 66, 110, 324
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 231, 251
MluCI AATT 4 cut(s) 59, 263, 452, 492
MmeI TCCRAC 1 cut(s) 539
MnlI CCTC 8 cut(s) 99, 154, 170, 312, 455, 504, 536, 555
MseI TTAA 3 cut(s) 9, 389, 618
MspR9I CCNGG 2 cut(s) 164, 244
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 2 cut(s) 164, 244
NdeII GATC 1 cut(s) 433
NlaIII CATG 3 cut(s) 507, 532, 569
NlaIV GGNNCC 2 cut(s) 248, 413
NspI RCATGY 2 cut(s) 507, 569
PaeI GCATGC 1 cut(s) 569
PfeI GAWTC 1 cut(s) 366
PkrI GCNGC 2 cut(s) 139, 571
Psp6I CCWGG 2 cut(s) 162, 242
PspGI CCWGG 2 cut(s) 162, 242
PspN4I GGNNCC 2 cut(s) 248, 413
PspPI GGNCC 2 cut(s) 412, 425
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 1 cut(s) 615
RsaNI GTAC 1 cut(s) 614
SaqAI TTAA 3 cut(s) 9, 389, 618
SatI GCNGC 2 cut(s) 138, 570
Sau3AI GATC 1 cut(s) 433
Sau96I GGNCC 2 cut(s) 412, 425
ScaI AGTACT 1 cut(s) 615
ScrFI CCNGG 2 cut(s) 164, 244
SduI GDGCHC 2 cut(s) 231, 251
SetI ASST 9 cut(s) 146, 168, 293, 299, 377, 407, 502, 515, 589
SfaNI GCATC 1 cut(s) 206
SinI GGWCC 2 cut(s) 412, 425
SmlI CTYRAG 2 cut(s) 303, 355
SmoI CTYRAG 2 cut(s) 303, 355
SphI GCATGC 1 cut(s) 569
Sse9I AATT 4 cut(s) 59, 263, 452, 492
SsiI CCGC 3 cut(s) 135, 138, 555
SspMI CTAG 2 cut(s) 372, 497
StyD4I CCNGG 2 cut(s) 162, 242
TaiI ACGT 2 cut(s) 299, 407
TasI AATT 4 cut(s) 59, 263, 452, 492
TatI WGTACW 1 cut(s) 613
TauI GCSGC 1 cut(s) 140
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 3 cut(s) 9, 389, 618
Tru9I TTAA 3 cut(s) 9, 389, 618
TscAI CASTG 1 cut(s) 217
TseI GCWGC 1 cut(s) 569
TspDTI ATGAA 2 cut(s) 169, 589
TspGWI ACGGA 2 cut(s) 98, 220
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 2 cut(s) 412, 425
XceI RCATGY 2 cut(s) 507, 569
XspI CTAG 2 cut(s) 372, 497
ZraI GACGTC 1 cut(s) 405
ZrmI AGTACT 1 cut(s) 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.