FvH4_1g28240

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008614
Physical Location & Seq
Reverse (-)
20069625 .. 20077317
7693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g28240.t1

Sequence Viewer

Length: 909 bp
ATGGGTTGTTGTTCTAAATCTGTACTCTTGTTCCTTTTGTTGTTGTTATGTATCATCACAACCCTGCAATTGGTATCGTCGTCGGATGTCTGCCCTTCCCAATCCCATTTGTTCCGTCTTACTCTTCAATCTCAATGCCCCATCCCTGTCTCCCCCTCCACTCCCCTCCAGGTGGATGGGGACTTCATTGATAGAGTGTTGGCATCAAAACAGAATTCTGGTTTTACTTCTGTGCTCTTTTATGCTTCCTGGTGCCCGTTTTCACGCAGGTTGAATCCTACTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGATCATTTGGCAGTAGAGCAATCTTCAGCCTTCCCAAGTGTGTTCTCAAGATATGGTATCCCTAGCTTCCCTTCTATTTTAGTAGTCAATCATACATCAAGGGTGCGATATCATGGCCCAAAAGATCTCCTGTCCCTTGTACAGTTTTACCAGAAAACCACAGGACTTGAGCCAGTTCAACATTACCATCATGATCAATCCGTCAGTTACGAACGCGCTGAAAAATCCATAATCCGATCAATGAATAATATGCCGTCATTAAAAGAGGTTTCAAGGAGAGAACCCTATTTAGTTTTCGCTACATTGTTTCTCTGTCTTAGGTTACTTCTACATATACTGCCTAAGGTATTGACTCACCTGAAAGCCTTTTGGGTTCTATACATTCCCCATTTCAACATGGGAATATTTGGGGAAACGAGTCAAATCATGGGGCGTATCCTCCACATGGTTGATGTTAGGAGGATTTGGAACAAGCTGAGATTATGCAAGACCAGGAATTTCCATGAAGGTGCAAAGAATGCCAGGGTTTGGGCTTCTTCCCTGACTTCAGTCTCGTTGGGTGAATCCTCATCGGCTAGATCATTGTCATCTTAA

Protein Analysis

303

Amino Acids

34.32

Weight (kDa)

9.53

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 73 - 155 7.1e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 258
AccB1I GGYRCC 1 cut(s) 252
AccB7I CCANNNNNTGG 1 cut(s) 843
AccII CGCG 1 cut(s) 531
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 2 cut(s) 214, 811
AcuI CTGAAG 2 cut(s) 324, 846
AfaI GTAC 2 cut(s) 24, 456
AfiI CCNNNNNNNGG 5 cut(s) 70, 172, 313, 760, 843
AgsI TTSAA 6 cut(s) 128, 274, 287, 494, 588, 709
AjnI CCWGG 4 cut(s) 168, 248, 806, 836
AluBI AGCT 3 cut(s) 297, 381, 790
AluI AGCT 3 cut(s) 297, 381, 790
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 2 cut(s) 154, 871
AlwI GGATC 1 cut(s) 324
AoxI GGCC 1 cut(s) 430
ApoI RAATTY 2 cut(s) 214, 811
AspLEI GCGC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 662, 887
AxyI CCTNAGG 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 257
BanI GGYRCC 1 cut(s) 252
Bbv12I GWGCWC 1 cut(s) 237
BccI CCATC 3 cut(s) 149, 170, 510
BceAI ACGGC 1 cut(s) 553
BciT130I CCWGG 4 cut(s) 170, 250, 808, 838
BciVI GTATCC 2 cut(s) 383, 761
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 2 cut(s) 154, 871
BfaI CTAG 2 cut(s) 378, 891
BfuAI ACCTGC 1 cut(s) 258
BfuI GTATCC 2 cut(s) 383, 761
BglII AGATCT 1 cut(s) 439
BlpI GCTNAGC 1 cut(s) 293
Bme1390I CCNGG 4 cut(s) 170, 250, 808, 838
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 4 cut(s) 170, 250, 808, 838
BmsI GCATC 1 cut(s) 212
BoxI GACNNNNGTC 1 cut(s) 863
BpmI CTGGAG 1 cut(s) 152
Bpu1102I GCTNAGC 1 cut(s) 293
BpuEI CTTGAG 3 cut(s) 294, 346, 503
BsaJI CCNNGG 1 cut(s) 837
Bsc4I CCNNNNNNNGG 5 cut(s) 70, 172, 313, 760, 843
Bse1I ACTGG 1 cut(s) 488
Bse21I CCTNAGG 1 cut(s) 657
BseBI CCWGG 4 cut(s) 170, 250, 808, 838
BseDI CCNNGG 1 cut(s) 837
BseGI GGATG 3 cut(s) 91, 141, 181
BseLI CCNNNNNNNGG 5 cut(s) 70, 172, 313, 760, 843
BseMII CTCAG 1 cut(s) 782
BseNI ACTGG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 257
Bsh1236I CGCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 432
BshNI GGYRCC 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 237
BslFI GGGAC 2 cut(s) 194, 433
BslI CCNNNNNNNGG 5 cut(s) 70, 172, 313, 760, 843
BsmAI GTCTC 2 cut(s) 154, 871
BsmFI GGGAC 2 cut(s) 194, 433
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 1 cut(s) 432
Bsp1286I GDGCHC 2 cut(s) 237, 257
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 5 cut(s) 316, 439, 508, 551, 893
Bsp1720I GCTNAGC 1 cut(s) 293
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 1 cut(s) 783
BspFNI CGCG 1 cut(s) 531
BspHI TCATGA 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 254
BspMI ACCTGC 1 cut(s) 258
BspPI GGATC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 252
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 488
BssECI CCNNGG 1 cut(s) 837
BssMI GATC 5 cut(s) 316, 439, 508, 551, 893
Bst2UI CCWGG 4 cut(s) 170, 250, 808, 838
Bst4CI ACNGT 1 cut(s) 459
Bst6I CTCTTC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 833
BstAUI TGTACA 1 cut(s) 454
BstDEI CTNAG 4 cut(s) 293, 632, 657, 791
BstF5I GGATG 3 cut(s) 91, 141, 181
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 533
BstKTI GATC 5 cut(s) 319, 442, 511, 554, 896
BstMAI GTCTC 2 cut(s) 154, 871
BstMBI GATC 5 cut(s) 316, 439, 508, 551, 893
BstMWI GCNNNNNNNGC 1 cut(s) 833
BstNI CCWGG 4 cut(s) 170, 250, 808, 838
BstPAI GACNNNNGTC 1 cut(s) 863
BstSCI CCNGG 4 cut(s) 168, 248, 806, 836
BstSLI GKGCMC 1 cut(s) 257
BstUI CGCG 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 439
BstXI CCANNNNNNTGG 1 cut(s) 176
BstYI RGATCY 1 cut(s) 439
Bsu36I CCTNAGG 1 cut(s) 657
BsuI GTATCC 2 cut(s) 383, 761
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 3 cut(s) 91, 141, 181
BveI ACCTGC 1 cut(s) 258
CciI TCATGA 1 cut(s) 505
CfoI GCGC 1 cut(s) 533
Cfr13I GGNCC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 23, 455
CspCI CAANNNNNGTGG 2 cut(s) 463, 498
CviAII CATG 7 cut(s) 301, 428, 506, 712, 742, 760, 818
CviJI RGCY 9 cut(s) 297, 344, 381, 432, 487, 680, 790, 848, 890
CviKI_1 RGCY 9 cut(s) 297, 344, 381, 432, 487, 680, 790, 848, 890
CviQI GTAC 2 cut(s) 23, 455
DdeI CTNAG 4 cut(s) 293, 632, 657, 791
DpnI GATC 5 cut(s) 318, 441, 510, 553, 895
DpnII GATC 5 cut(s) 316, 439, 508, 551, 893
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 425
Eco57I CTGAAG 2 cut(s) 324, 846
Eco81I CCTNAGG 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 214
EcoRII CCWGG 4 cut(s) 168, 248, 806, 836
EcoRV GATATC 1 cut(s) 425
FaeI CATG 7 cut(s) 304, 431, 509, 715, 745, 763, 821
FaqI GGGAC 2 cut(s) 194, 433
FatI CATG 7 cut(s) 300, 427, 505, 711, 741, 759, 817
FbaI TGATCA 1 cut(s) 508
FokI GGATG 3 cut(s) 98, 128, 188
FspBI CTAG 2 cut(s) 378, 891
GlaI GCGC 1 cut(s) 532
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 1 cut(s) 432
HhaI GCGC 1 cut(s) 533
Hin1II CATG 7 cut(s) 304, 431, 509, 715, 745, 763, 821
Hin6I GCGC 1 cut(s) 531
HinP1I GCGC 1 cut(s) 531
HinfI GANTC 4 cut(s) 274, 667, 733, 878
HphI GGTGA 2 cut(s) 662, 887
Hpy188I TCNGA 2 cut(s) 85, 551
Hpy188III TCNNGA 2 cut(s) 363, 506
Hpy99I CGWCG 2 cut(s) 82, 85
HpyAV CCTTC 4 cut(s) 105, 355, 396, 815
HpyCH4III ACNGT 1 cut(s) 459
HpyCH4V TGCA 3 cut(s) 67, 801, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 833
HpyF3I CTNAG 4 cut(s) 293, 632, 657, 791
Hsp92II CATG 7 cut(s) 304, 431, 509, 715, 745, 763, 821
HspAI GCGC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 5 cut(s) 316, 439, 508, 551, 893
LweI GCATC 1 cut(s) 212
MaeI CTAG 2 cut(s) 378, 891
MaeIII GTNAC 2 cut(s) 521, 636
MalI GATC 5 cut(s) 318, 441, 510, 553, 895
MboI GATC 5 cut(s) 316, 439, 508, 551, 893
MboII GAAGA 3 cut(s) 116, 330, 843
MfeI CAATTG 1 cut(s) 68
MflI RGATCY 1 cut(s) 439
MhlI GDGCHC 2 cut(s) 237, 257
MluCI AATT 3 cut(s) 68, 214, 811
MlyI GAGTC 2 cut(s) 661, 742
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 7 cut(s) 166, 176, 318, 574, 764, 768, 892
MseI TTAA 2 cut(s) 575, 907
MslI CAYNNNNRTG 1 cut(s) 822
MspR9I CCNGG 4 cut(s) 170, 250, 808, 838
MunI CAATTG 1 cut(s) 68
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 4 cut(s) 170, 250, 808, 838
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 833
NdeII GATC 5 cut(s) 316, 439, 508, 551, 893
NlaIII CATG 7 cut(s) 304, 431, 509, 715, 745, 763, 821
NlaIV GGNNCC 1 cut(s) 254
PagI TCATGA 1 cut(s) 505
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 2 cut(s) 274, 878
PflMI CCANNNNNTGG 1 cut(s) 843
PleI GAGTC 2 cut(s) 661, 741
PpsI GAGTC 2 cut(s) 661, 741
PshAI GACNNNNGTC 1 cut(s) 863
Psp6I CCWGG 4 cut(s) 168, 248, 806, 836
PspGI CCWGG 4 cut(s) 168, 248, 806, 836
PspN4I GGNNCC 1 cut(s) 254
PspPI GGNCC 1 cut(s) 431
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 2 cut(s) 24, 456
RsaNI GTAC 2 cut(s) 23, 455
RseI CAYNNNNRTG 1 cut(s) 822
SaqAI TTAA 2 cut(s) 575, 907
Sau3AI GATC 5 cut(s) 316, 439, 508, 551, 893
Sau96I GGNCC 1 cut(s) 431
SchI GAGTC 2 cut(s) 661, 742
ScrFI CCNGG 4 cut(s) 170, 250, 808, 838
SduI GDGCHC 2 cut(s) 237, 257
SfaNI GCATC 1 cut(s) 212
SmiMI CAYNNNNRTG 1 cut(s) 822
SmlI CTYRAG 3 cut(s) 309, 361, 482
SmoI CTYRAG 3 cut(s) 309, 361, 482
Sse9I AATT 3 cut(s) 68, 214, 811
SspI AATATT 1 cut(s) 720
SspMI CTAG 2 cut(s) 378, 891
StyD4I CCNGG 4 cut(s) 168, 248, 806, 836
TaaI ACNGT 1 cut(s) 459
TasI AATT 3 cut(s) 68, 214, 811
TatI WGTACW 2 cut(s) 22, 454
TfiI GAWTC 2 cut(s) 274, 878
Tru1I TTAA 2 cut(s) 575, 907
Tru9I TTAA 2 cut(s) 575, 907
TspDTI ATGAA 4 cut(s) 175, 289, 572, 834
TspGWI ACGGA 2 cut(s) 104, 505
Van91I CCANNNNNTGG 1 cut(s) 843
XapI RAATTY 2 cut(s) 214, 811
XspI CTAG 2 cut(s) 378, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.