Rw6G014640

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008614
Physical Location & Seq
Forward (+)
27173998 .. 27178560
4563 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014640.1

Sequence Viewer

Length: 669 bp
ATGGGTTTTAAATCTGCAATCTTCTTGTTGTTGTTATATATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTGTCTGCCCTCCCCAGTCCGATTTGTTCCGTATCACTCTTCAATCTCAATGCCCCGCCGCAACCTCCCCCTACCCACCCCTCCAGGTGGATGGGAACTTCATTGACAGAGCGTTAGCATCAACACGGAACACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGAGCATTTAGCAGTTGAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTTTGGTCCAAAAGATCTCCTGTCCCTCGTCCAATTTTACCAGAAAACCACAGTTCTTAATCACGTAGCATCTTTGGCTGCCACAAGGCAACACCTTCCGGGTACCAAGTGCCTTCCAAAAATTTCCCAAACTTACCCAAAAGTTTCTTTGGTAAAAATTAGGTTTTGGACTAACAATCCGCTCGGTGGATCGTTGTGCGGCGAAAAGTGGAACTATTTTGACACAGTCACAAACCCGAAGCAGTTGTAA

Protein Analysis

222

Amino Acids

24.95

Weight (kDa)

9.53

Isoelectric Point (pI)

46.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 75 - 153 2.5e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 407
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 2 cut(s) 246, 521
AccBSI CCGCTC 1 cut(s) 601
AciI CCGC 4 cut(s) 135, 138, 599, 618
AclWI GGATC 1 cut(s) 616
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 1 cut(s) 318
AcyI GRCGYC 1 cut(s) 404
AfaI GTAC 1 cut(s) 523
AfiI CCNNNNNNNGG 3 cut(s) 166, 307, 605
AgsI TTSAA 2 cut(s) 122, 281
AjnI CCWGG 2 cut(s) 162, 242
AluBI AGCT 2 cut(s) 291, 375
AluI AGCT 2 cut(s) 291, 375
Alw21I GWGCWC 1 cut(s) 231
AlwI GGATC 1 cut(s) 616
ApeKI GCWGC 1 cut(s) 497
ApoI RAATTY 1 cut(s) 540
Asp718I GGTACC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 412, 425
AsuC2I CCSGG 1 cut(s) 519
AvaII GGWCC 2 cut(s) 412, 425
BaeGI GKGCMC 1 cut(s) 251
BanI GGYRCC 2 cut(s) 246, 521
BbsI GAAGAC 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 1 cut(s) 484
BccI CCATC 1 cut(s) 164
BciT130I CCWGG 2 cut(s) 164, 244
BciVI GTATCC 1 cut(s) 279
BcnI CCSGG 1 cut(s) 519
BfaI CTAG 1 cut(s) 372
BfuI GTATCC 1 cut(s) 279
BglII AGATCT 1 cut(s) 433
BisI GCNGC 3 cut(s) 138, 498, 619
BlpI GCTNAGC 1 cut(s) 287
BlsI GCNGC 3 cut(s) 139, 499, 620
Bme1390I CCNGG 3 cut(s) 164, 244, 519
Bme18I GGWCC 2 cut(s) 412, 425
BmgT120I GGNCC 2 cut(s) 412, 425
BmiI GGNNCC 3 cut(s) 248, 413, 523
BmrFI CCNGG 3 cut(s) 164, 244, 519
BmrI ACTGGG 1 cut(s) 88
BmsI GCATC 2 cut(s) 206, 497
BmuI ACTGGG 1 cut(s) 88
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 1 cut(s) 146
Bpu1102I GCTNAGC 1 cut(s) 287
BpuEI CTTGAG 2 cut(s) 288, 340
BpuMI CCSGG 1 cut(s) 519
BsaAI YACGTR 1 cut(s) 484
BsaHI GRCGYC 1 cut(s) 404
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 307, 605
Bse1I ACTGG 2 cut(s) 94, 217
BseBI CCWGG 2 cut(s) 164, 244
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 3 cut(s) 166, 307, 605
BseNI ACTGG 2 cut(s) 94, 217
BseSI GKGCMC 1 cut(s) 251
BseXI GCAGC 1 cut(s) 484
BshNI GGYRCC 2 cut(s) 246, 521
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 1 cut(s) 518
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 3 cut(s) 166, 307, 605
BsmFI GGGAC 1 cut(s) 427
Bsp1286I GDGCHC 2 cut(s) 231, 251
Bsp143I GATC 2 cut(s) 433, 608
Bsp1720I GCTNAGC 1 cut(s) 287
BspACI CCGC 4 cut(s) 135, 138, 599, 618
BspLI GGNNCC 3 cut(s) 248, 413, 523
BspPI GGATC 1 cut(s) 616
BspT107I GGYRCC 2 cut(s) 246, 521
BsrBI CCGCTC 1 cut(s) 601
BsrI ACTGG 2 cut(s) 94, 217
BssMI GATC 2 cut(s) 433, 608
BssNI GRCGYC 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 164, 244
Bst4CI ACNGT 2 cut(s) 472, 646
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 1 cut(s) 404
BstBAI YACGTR 1 cut(s) 484
BstDEI CTNAG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 2 cut(s) 436, 611
BstMBI GATC 2 cut(s) 433, 608
BstMWI GCNNNNNNNGC 1 cut(s) 494
BstNI CCWGG 2 cut(s) 164, 244
BstSCI CCNGG 3 cut(s) 162, 242, 517
BstSLI GKGCMC 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 484
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 170
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 1 cut(s) 279
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 210
Cfr13I GGNCC 2 cut(s) 412, 425
Csp6I GTAC 1 cut(s) 522
CviAII CATG 1 cut(s) 295
CviJI RGCY 4 cut(s) 291, 338, 375, 497
CviKI_1 RGCY 4 cut(s) 291, 338, 375, 497
CviQI GTAC 1 cut(s) 522
DdeI CTNAG 1 cut(s) 287
DpnI GATC 2 cut(s) 435, 610
DpnII GATC 2 cut(s) 433, 608
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
Eco47I GGWCC 2 cut(s) 412, 425
Eco57I CTGAAG 1 cut(s) 318
EcoRII CCWGG 2 cut(s) 162, 242
FaeI CATG 1 cut(s) 298
FaiI YATR 5 cut(s) 37, 39, 219, 296, 363
FaqI GGGAC 1 cut(s) 427
FatI CATG 1 cut(s) 294
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 3 cut(s) 138, 498, 619
FokI GGATG 1 cut(s) 182
Fsp4HI GCNGC 3 cut(s) 138, 498, 619
FspBI CTAG 1 cut(s) 372
GluI GCNGC 3 cut(s) 138, 498, 619
GsuI CTGGAG 1 cut(s) 146
HapII CCGG 1 cut(s) 518
Hin1I GRCGYC 1 cut(s) 404
Hin1II CATG 1 cut(s) 298
HinfI GANTC 1 cut(s) 366
HpaII CCGG 1 cut(s) 518
Hpy188I TCNGA 3 cut(s) 100, 402, 416
Hpy188III TCNNGA 1 cut(s) 357
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 349, 390, 524, 542
HpyCH4III ACNGT 2 cut(s) 472, 646
HpyCH4IV ACGT 2 cut(s) 404, 483
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 1 cut(s) 494
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 2 cut(s) 404, 483
Hsp92I GRCGYC 1 cut(s) 404
Hsp92II CATG 1 cut(s) 298
KpnI GGTACC 1 cut(s) 525
Kzo9I GATC 2 cut(s) 433, 608
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 484
LweI GCATC 2 cut(s) 206, 497
MaeI CTAG 1 cut(s) 372
MaeII ACGT 2 cut(s) 404, 483
MaeIII GTNAC 1 cut(s) 646
MalI GATC 2 cut(s) 435, 610
MbiI CCGCTC 1 cut(s) 601
MboI GATC 2 cut(s) 433, 608
MboII GAAGA 4 cut(s) 13, 66, 110, 324
MfeI CAATTG 1 cut(s) 59
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 2 cut(s) 231, 251
MluCI AATT 5 cut(s) 59, 263, 452, 540, 576
MnlI CCTC 5 cut(s) 99, 154, 170, 312, 455
MseI TTAA 3 cut(s) 9, 389, 477
MspI CCGG 1 cut(s) 518
MspR9I CCNGG 3 cut(s) 164, 244, 519
MunI CAATTG 1 cut(s) 59
MvaI CCWGG 2 cut(s) 164, 244
MwoI GCNNNNNNNGC 1 cut(s) 494
NciI CCSGG 1 cut(s) 519
NdeII GATC 2 cut(s) 433, 608
NlaIII CATG 1 cut(s) 298
NlaIV GGNNCC 3 cut(s) 248, 413, 523
NmuCI GTSAC 1 cut(s) 646
PfeI GAWTC 1 cut(s) 366
PflFI GACNNNGTC 1 cut(s) 644
PkrI GCNGC 3 cut(s) 139, 499, 620
Ppu21I YACGTR 1 cut(s) 484
Psp6I CCWGG 2 cut(s) 162, 242
PspGI CCWGG 2 cut(s) 162, 242
PspN4I GGNNCC 3 cut(s) 248, 413, 523
PspPI GGNCC 2 cut(s) 412, 425
PsuI RGATCY 1 cut(s) 433
PsyI GACNNNGTC 1 cut(s) 644
RsaI GTAC 1 cut(s) 523
RsaNI GTAC 1 cut(s) 522
SaqAI TTAA 3 cut(s) 9, 389, 477
SatI GCNGC 3 cut(s) 138, 498, 619
Sau3AI GATC 2 cut(s) 433, 608
Sau96I GGNCC 2 cut(s) 412, 425
ScrFI CCNGG 3 cut(s) 164, 244, 519
SduI GDGCHC 2 cut(s) 231, 251
SetI ASST 8 cut(s) 146, 168, 293, 377, 407, 486, 516, 584
SfaNI GCATC 2 cut(s) 206, 497
SinI GGWCC 2 cut(s) 412, 425
SmlI CTYRAG 2 cut(s) 303, 355
SmoI CTYRAG 2 cut(s) 303, 355
Sse9I AATT 5 cut(s) 59, 263, 452, 540, 576
SsiI CCGC 4 cut(s) 135, 138, 599, 618
SspMI CTAG 1 cut(s) 372
StyD4I CCNGG 3 cut(s) 162, 242, 517
TaaI ACNGT 2 cut(s) 472, 646
TaiI ACGT 2 cut(s) 407, 486
TasI AATT 5 cut(s) 59, 263, 452, 540, 576
TauI GCSGC 2 cut(s) 140, 621
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 3 cut(s) 9, 389, 477
Tru9I TTAA 3 cut(s) 9, 389, 477
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 1 cut(s) 497
Tsp45I GTSAC 1 cut(s) 646
TspDTI ATGAA 2 cut(s) 169, 283
TspGWI ACGGA 2 cut(s) 98, 220
TspRI CASTG 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 2 cut(s) 412, 425
XapI RAATTY 1 cut(s) 540
XspI CTAG 1 cut(s) 372
ZraI GACGTC 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.