MD12G1005000.v1.1

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0008614
Physical Location & Seq
Forward (+)
634369 .. 639729
5361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1005000.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGATAAATCTGTAATATTGTTGTATATCGCTGCCTTGGCAGCTTCGTTTCGATTAGCTTCTTCTTCTGCTCTCTGCCCTCACGAGTCCGATTTCTTCCGTTACAGCCTTCAATCTCAATGCCCCATCTCGATCTCCCCCCACCCACCCCTCAAGGTGGATGGAAACTTCCTAGACAGAGCTTTGGCATCACAACAGAGAACTGATTATTCTGCAGTGCTATTTTATGCTTCCTGGTGCCCACTTTCACGCACATTGTATCCCACATTTGAAAAGCTCAGCTTCATGTTCCCTCAAGTAGAGCATTTGGCCATTGAGGAATCTTCAGCCCTGCCGAGTGTGTTCTCAAGATATGGAATACATAGCTTCCCTTCGATTTTACTAGTCAATCAGACATCAATGGTGCGTTATCATGGTCCAAAAAATCTCTCCTTGCTTGCACAGTTTTACCAGAAAACCACAGGACTTGAACCAGTTCAATATTTTGATGGTGATCATACTGTTAGCTTAAATATACGTGAAAAATCTATAATCCAGTCAATGAGTGACATGTCACTAAGGGAGATATCAAGGAGGGAACCCTTTTTAGCTTTTGCTACATTGTTTCTCTGTTTGAGGGTGCTTCTGTATATGTTCCCGAAGGTATTGACTCGCCTCCAAGCTTTCTGGGTCCTATACGTTCCTCATTTTAACTTGGGAATATTTGGGGAGACGAGTCAAATTATGGGGCGTATCCTCCACATGGTTGATGTTAGGAGGATTTGGACCAAGCTGAGATTATGCAAGACGAGGAACTTCCACAAGGGAGCAAAGAACGCCAGGGTTTGGGCTTCTTCCCTAACTTCTGTCTCCTTGGGCAAATCTTCATCTGCTCGATCATCGACAAACTGA

Protein Analysis

297

Amino Acids

33.68

Weight (kDa)

9.7

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 69 - 150 1.1e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 825
AcoI YGGCCR 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 309
AfiI CCNNNNNNNGG 3 cut(s) 157, 742, 825
AflIII ACRYGT 1 cut(s) 549
AgsI TTSAA 4 cut(s) 113, 272, 470, 479
AhlI ACTAGT 1 cut(s) 382
AjnI CCWGG 2 cut(s) 233, 818
Alw26I GTCTC 2 cut(s) 704, 853
AoxI GGCC 1 cut(s) 309
ApeKI GCWGC 2 cut(s) 32, 41
Asp700I GAANNNNTTC 1 cut(s) 474
AspS9I GGNCC 3 cut(s) 416, 670, 765
AsuHPI GGTGA 1 cut(s) 503
AvaII GGWCC 3 cut(s) 416, 670, 765
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 1 cut(s) 311
BanI GGYRCC 1 cut(s) 237
BauI CACGAG 1 cut(s) 83
BbvI GCAGC 2 cut(s) 19, 53
BccI CCATC 3 cut(s) 134, 155, 482
BciT130I CCWGG 2 cut(s) 235, 820
BciVI GTATCC 2 cut(s) 270, 743
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 704, 853
BcuI ACTAGT 1 cut(s) 382
BfaI CTAG 2 cut(s) 173, 383
BfmI CTRYAG 1 cut(s) 213
BfuI GTATCC 2 cut(s) 270, 743
BisI GCNGC 2 cut(s) 33, 42
BlpI GCTNAGC 1 cut(s) 278
BlsI GCNGC 2 cut(s) 34, 43
Bme1390I CCNGG 2 cut(s) 235, 820
Bme18I GGWCC 3 cut(s) 416, 670, 765
BmgT120I GGNCC 3 cut(s) 416, 670, 765
BmiI GGNNCC 3 cut(s) 239, 579, 671
BmrFI CCNGG 2 cut(s) 235, 820
BmsI GCATC 1 cut(s) 197
Bpu1102I GCTNAGC 1 cut(s) 278
BpuEI CTTGAG 3 cut(s) 137, 279, 331
BsaAI YACGTR 1 cut(s) 518
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 36, 819, 852
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 742, 825
Bse1I ACTGG 2 cut(s) 473, 535
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 2 cut(s) 235, 820
BseDI CCNNGG 3 cut(s) 36, 819, 852
BseGI GGATG 1 cut(s) 166
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 3 cut(s) 157, 742, 825
BseMII CTCAG 2 cut(s) 292, 764
BseNI ACTGG 2 cut(s) 473, 535
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 2 cut(s) 19, 53
BshFI GGCC 1 cut(s) 311
BshNI GGYRCC 1 cut(s) 237
BslI CCNNNNNNNGG 3 cut(s) 157, 742, 825
BsmAI GTCTC 2 cut(s) 704, 853
BsmBI CGTCTC 1 cut(s) 704
BsnI GGCC 1 cut(s) 311
Bsp1286I GDGCHC 1 cut(s) 242
Bsp143I GATC 3 cut(s) 132, 493, 875
Bsp1720I GCTNAGC 1 cut(s) 278
BspANI GGCC 1 cut(s) 311
BspCNI CTCAG 2 cut(s) 291, 765
BspLI GGNNCC 3 cut(s) 239, 579, 671
BspMAI CTGCAG 1 cut(s) 217
BspT107I GGYRCC 1 cut(s) 237
BsrI ACTGG 2 cut(s) 473, 535
BssECI CCNNGG 3 cut(s) 36, 819, 852
BssMI GATC 3 cut(s) 132, 493, 875
BssSI CACGAG 1 cut(s) 83
BssT1I CCWWGG 2 cut(s) 36, 852
Bst2BI CACGAG 1 cut(s) 83
Bst2UI CCWGG 2 cut(s) 235, 820
Bst4CI ACNGT 2 cut(s) 444, 502
BstBAI YACGTR 1 cut(s) 518
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 3 cut(s) 278, 557, 773
BstF5I GGATG 1 cut(s) 166
BstKTI GATC 3 cut(s) 135, 496, 878
BstMAI GTCTC 2 cut(s) 704, 853
BstMBI GATC 3 cut(s) 132, 493, 875
BstMWI GCNNNNNNNGC 3 cut(s) 38, 41, 815
BstNI CCWGG 2 cut(s) 235, 820
BstNSI RCATGY 1 cut(s) 553
BstSCI CCNGG 2 cut(s) 233, 818
BstSFI CTRYAG 1 cut(s) 213
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 2 cut(s) 19, 53
BsuI GTATCC 2 cut(s) 270, 743
BsuRI GGCC 1 cut(s) 311
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 1 cut(s) 222
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 438
Cfr13I GGNCC 3 cut(s) 416, 670, 765
CspCI CAANNNNNGTGG 2 cut(s) 448, 483
CviAII CATG 4 cut(s) 286, 413, 550, 742
DdeI CTNAG 3 cut(s) 278, 557, 773
DpnI GATC 3 cut(s) 134, 495, 877
DpnII GATC 3 cut(s) 132, 493, 875
EaeI YGGCCR 1 cut(s) 309
Eco130I CCWWGG 2 cut(s) 36, 852
Eco32I GATATC 1 cut(s) 567
Eco47I GGWCC 3 cut(s) 416, 670, 765
Eco57I CTGAAG 1 cut(s) 309
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 233, 818
EcoRV GATATC 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 36, 852
ErhI CCWWGG 2 cut(s) 36, 852
Esp3I CGTCTC 1 cut(s) 704
FaeI CATG 4 cut(s) 289, 416, 553, 745
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FatI CATG 4 cut(s) 285, 412, 549, 741
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 2 cut(s) 33, 42
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 2 cut(s) 33, 42
FspBI CTAG 2 cut(s) 173, 383
GluI GCNGC 2 cut(s) 33, 42
HaeIII GGCC 1 cut(s) 311
Hin1II CATG 4 cut(s) 289, 416, 553, 745
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 4 cut(s) 86, 320, 649, 715
HphI GGTGA 1 cut(s) 503
Hpy188I TCNGA 2 cut(s) 91, 393
Hpy188III TCNNGA 4 cut(s) 83, 130, 348, 637
HpyAV CCTTC 3 cut(s) 119, 381, 634
HpyCH4III ACNGT 2 cut(s) 444, 502
HpyCH4IV ACGT 2 cut(s) 517, 678
HpyCH4V TGCA 3 cut(s) 215, 440, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 41, 815
HpyF3I CTNAG 3 cut(s) 278, 557, 773
HpySE526I ACGT 2 cut(s) 517, 678
Hsp92II CATG 4 cut(s) 289, 416, 553, 745
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 3 cut(s) 132, 493, 875
LmnI GCTCC 1 cut(s) 806
Lsp1109I GCAGC 2 cut(s) 19, 53
LweI GCATC 1 cut(s) 197
MaeI CTAG 2 cut(s) 173, 383
MaeII ACGT 2 cut(s) 517, 678
MaeIII GTNAC 3 cut(s) 101, 545, 552
MalI GATC 3 cut(s) 134, 495, 877
MboI GATC 3 cut(s) 132, 493, 875
MboII GAAGA 6 cut(s) 54, 57, 88, 315, 825, 855
MhlI GDGCHC 1 cut(s) 242
MlsI TGGCCA 1 cut(s) 311
MluCI AATT 1 cut(s) 720
MluNI TGGCCA 1 cut(s) 311
MlyI GAGTC 3 cut(s) 95, 643, 724
Mox20I TGGCCA 1 cut(s) 311
MroXI GAANNNNTTC 1 cut(s) 474
MscI TGGCCA 1 cut(s) 311
MseI TTAA 2 cut(s) 509, 690
Msp20I TGGCCA 1 cut(s) 311
MspR9I CCNGG 2 cut(s) 235, 820
MvaI CCWGG 2 cut(s) 235, 820
MwoI GCNNNNNNNGC 3 cut(s) 38, 41, 815
NdeII GATC 3 cut(s) 132, 493, 875
NlaIII CATG 4 cut(s) 289, 416, 553, 745
NlaIV GGNNCC 3 cut(s) 239, 579, 671
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 2 cut(s) 545, 552
NspI RCATGY 1 cut(s) 553
PciI ACATGT 1 cut(s) 549
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 320
PflMI CCANNNNNTGG 1 cut(s) 825
PkrI GCNGC 2 cut(s) 34, 43
PleI GAGTC 3 cut(s) 94, 643, 723
PpsI GAGTC 3 cut(s) 94, 643, 723
Ppu21I YACGTR 1 cut(s) 518
PpuMI RGGWCCY 1 cut(s) 670
PscI ACATGT 1 cut(s) 549
Psp5II RGGWCCY 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 233, 818
PspGI CCWGG 2 cut(s) 233, 818
PspN4I GGNNCC 3 cut(s) 239, 579, 671
PspPI GGNCC 3 cut(s) 416, 670, 765
PspPPI RGGWCCY 1 cut(s) 670
PstI CTGCAG 1 cut(s) 217
SaqAI TTAA 2 cut(s) 509, 690
SatI GCNGC 2 cut(s) 33, 42
Sau3AI GATC 3 cut(s) 132, 493, 875
Sau96I GGNCC 3 cut(s) 416, 670, 765
SchI GAGTC 3 cut(s) 95, 643, 724
ScrFI CCNGG 2 cut(s) 235, 820
SduI GDGCHC 1 cut(s) 242
SfaNI GCATC 1 cut(s) 197
SfcI CTRYAG 1 cut(s) 213
SinI GGWCC 3 cut(s) 416, 670, 765
SmlI CTYRAG 3 cut(s) 152, 294, 346
SmoI CTYRAG 3 cut(s) 152, 294, 346
SpeI ACTAGT 1 cut(s) 382
Sse9I AATT 1 cut(s) 720
SspI AATATT 3 cut(s) 18, 482, 702
SspMI CTAG 2 cut(s) 173, 383
StyD4I CCNGG 2 cut(s) 233, 818
StyI CCWWGG 2 cut(s) 36, 852
TaaI ACNGT 2 cut(s) 444, 502
TaiI ACGT 2 cut(s) 520, 681
TaqI TCGA 5 cut(s) 52, 131, 374, 874, 881
TasI AATT 1 cut(s) 720
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 2 cut(s) 509, 690
Tru9I TTAA 2 cut(s) 509, 690
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 2 cut(s) 545, 552
TseI GCWGC 2 cut(s) 32, 41
Tsp45I GTSAC 2 cut(s) 545, 552
TspDTI ATGAA 2 cut(s) 274, 855
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 222
Van91I CCANNNNNTGG 1 cut(s) 825
VpaK11BI GGWCC 3 cut(s) 416, 670, 765
XceI RCATGY 1 cut(s) 553
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 2 cut(s) 173, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.