Rroxscaffold_6G00388580

5-adenylylsulfate reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0008614
Physical Location & Seq
Reverse (-)
1591152 .. 1596989
5838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00388580.1

Sequence Viewer

Length: 900 bp
ATGGGTTTTAAATCTGCAATCTTGTTGTTGTTATACATCACAATCACAACGCTACAATTGGTATCGTCGTCTTCGCCTGTCTGCCCTCCCCAGTCCGATTTGTTCCGTATCACTCTTCAATCTCAATGCCCCGTCGCAACCTCCCCCTACCCACCCCTCCAGGTGGATGGGAACTTCATTGACAGAGCATTGGCATCAACACGGAACACTGGTTATACTTCTGTGCTCTTTTACGCTTCCTGGTGCCCATTTTCACGCAAATTGTATCCTATTTTTGAAATGCTTAGCTTCATGTTCCCTCAAGTGGAGCATTTAGCAGTTGAGCAATCTTCAGCCTTCCCAAGTGTATTCTCAAGATATGGAATCCCTAGCTTCCCTTCTATTTTAATAGTCAATCAGACGTCAAGGGTCCGATATTTTGGTCCAAAAGATCTCCTGTCCCTTGTCCAATTTTACCAGAAAACCACAGGACTTGAGCCGGTTCAGTATTACAATCATGATCAATCCATCGGTTGTGAATCTGGTGAAAAATCCATAATCAAATCAATGAGTAACCTGCTGTCACTAAGAGAGATATCAAGGAAGGAACCCTATCTAGTTTTCGCTATATTGTTTCTCTGTCTTAGGGCACTTATACATATATTGCCCAAGGTATTGACTCACCTGAAAGCTTTCTGGGTTCTATACGTTCCCCATTTCAACGTGGGAATATTTGGGGAGACGAGTCAAATTATGGGGCGTATCCTTCACATGGTTGATGTTAGGAGGATTTGGAACAAGCTGAGATTATGCAAGACCAGGAATTTCCATGAAGGTGCAAAGAATGCCAGGGTTTGGGCTTCTTCCCTGACTTCAGTCTCTTTGGGTGAATCTTCATCGGCTAGATCATGGTCATCTTAA

Protein Analysis

299

Amino Acids

33.88

Weight (kDa)

9.55

Isoelectric Point (pI)

54.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 75 - 151 4.6e-06 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 564
AccB1I GGYRCC 1 cut(s) 243
AccB7I CCANNNNNTGG 1 cut(s) 834
AcsI RAATTY 1 cut(s) 802
AcuI CTGAAG 2 cut(s) 315, 837
AcyI GRCGYC 1 cut(s) 401
AfiI CCNNNNNNNGG 4 cut(s) 163, 304, 751, 834
AgsI TTSAA 3 cut(s) 119, 278, 700
AjnI CCWGG 4 cut(s) 159, 239, 797, 827
AluBI AGCT 4 cut(s) 288, 372, 671, 781
AluI AGCT 4 cut(s) 288, 372, 671, 781
Alw21I GWGCWC 1 cut(s) 228
Alw26I GTCTC 2 cut(s) 713, 862
ApoI RAATTY 1 cut(s) 802
Asp700I GAANNNNTTC 1 cut(s) 671
AspS9I GGNCC 2 cut(s) 409, 422
AsuHPI GGTGA 3 cut(s) 536, 653, 878
AvaII GGWCC 2 cut(s) 409, 422
BaeGI GKGCMC 2 cut(s) 248, 631
BanI GGYRCC 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 228
BccI CCATC 2 cut(s) 161, 515
BciT130I CCWGG 4 cut(s) 161, 241, 799, 829
BciVI GTATCC 2 cut(s) 276, 752
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 713, 862
BfaI CTAG 3 cut(s) 369, 596, 882
BfuAI ACCTGC 1 cut(s) 564
BfuI GTATCC 2 cut(s) 276, 752
BglII AGATCT 1 cut(s) 430
BlpI GCTNAGC 1 cut(s) 284
Bme1390I CCNGG 4 cut(s) 161, 241, 799, 829
Bme18I GGWCC 2 cut(s) 409, 422
BmgT120I GGNCC 2 cut(s) 409, 422
BmiI GGNNCC 3 cut(s) 245, 410, 588
BmrFI CCNGG 4 cut(s) 161, 241, 799, 829
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 1 cut(s) 203
BmuI ACTGGG 1 cut(s) 85
BoxI GACNNNNGTC 1 cut(s) 854
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 284
BpuEI CTTGAG 3 cut(s) 285, 337, 494
BsaHI GRCGYC 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 648, 828
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 304, 751, 834
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 2 cut(s) 91, 214
BseBI CCWGG 4 cut(s) 161, 241, 799, 829
BseDI CCNNGG 2 cut(s) 648, 828
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 4 cut(s) 163, 304, 751, 834
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 2 cut(s) 91, 214
BseSI GKGCMC 2 cut(s) 248, 631
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 228
BsiSI CCGG 1 cut(s) 479
BslFI GGGAC 1 cut(s) 424
BslI CCNNNNNNNGG 4 cut(s) 163, 304, 751, 834
BsmAI GTCTC 2 cut(s) 713, 862
BsmBI CGTCTC 1 cut(s) 713
BsmFI GGGAC 1 cut(s) 424
BsmI GAATGC 1 cut(s) 829
Bsp1286I GDGCHC 3 cut(s) 228, 248, 631
Bsp143I GATC 3 cut(s) 430, 499, 884
Bsp1720I GCTNAGC 1 cut(s) 284
BspCNI CTCAG 1 cut(s) 774
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 3 cut(s) 245, 410, 588
BspMI ACCTGC 1 cut(s) 564
BspT107I GGYRCC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 2 cut(s) 91, 214
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 2 cut(s) 648, 828
BssMI GATC 3 cut(s) 430, 499, 884
BssNI GRCGYC 1 cut(s) 401
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 4 cut(s) 161, 241, 799, 829
Bst6I CTCTTC 1 cut(s) 120
BstACI GRCGYC 1 cut(s) 401
BstAPI GCANNNNNTGC 1 cut(s) 824
BstDEI CTNAG 4 cut(s) 284, 566, 623, 782
BstF5I GGATG 1 cut(s) 172
BstKTI GATC 3 cut(s) 433, 502, 887
BstMAI GTCTC 2 cut(s) 713, 862
BstMBI GATC 3 cut(s) 430, 499, 884
BstMWI GCNNNNNNNGC 1 cut(s) 824
BstNI CCWGG 4 cut(s) 161, 241, 799, 829
BstPAI GACNNNNGTC 1 cut(s) 854
BstSCI CCNGG 4 cut(s) 159, 239, 797, 827
BstSLI GKGCMC 2 cut(s) 248, 631
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 430
BstXI CCANNNNNNTGG 1 cut(s) 167
BstYI RGATCY 1 cut(s) 430
BsuI GTATCC 2 cut(s) 276, 752
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 1 cut(s) 207
BveI ACCTGC 1 cut(s) 564
CciI TCATGA 1 cut(s) 496
Cfr10I RCCGGY 1 cut(s) 478
Cfr13I GGNCC 2 cut(s) 409, 422
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 5 cut(s) 292, 497, 751, 809, 888
CviJI RGCY 8 cut(s) 288, 335, 372, 478, 671, 781, 839, 881
CviKI_1 RGCY 8 cut(s) 288, 335, 372, 478, 671, 781, 839, 881
DdeI CTNAG 4 cut(s) 284, 566, 623, 782
DpnI GATC 3 cut(s) 432, 501, 886
DpnII GATC 3 cut(s) 430, 499, 884
DraI TTTAAA 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 120
EarI CTCTTC 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 648
Eco32I GATATC 1 cut(s) 576
Eco47I GGWCC 2 cut(s) 409, 422
Eco57I CTGAAG 2 cut(s) 315, 837
EcoRII CCWGG 4 cut(s) 159, 239, 797, 827
EcoRV GATATC 1 cut(s) 576
EcoT14I CCWWGG 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 648
Esp3I CGTCTC 1 cut(s) 713
FaeI CATG 5 cut(s) 295, 500, 754, 812, 891
FaqI GGGAC 1 cut(s) 424
FatI CATG 5 cut(s) 291, 496, 750, 808, 887
FbaI TGATCA 1 cut(s) 499
FokI GGATG 1 cut(s) 179
FspBI CTAG 3 cut(s) 369, 596, 882
GsuI CTGGAG 1 cut(s) 143
HapII CCGG 1 cut(s) 479
Hin1I GRCGYC 1 cut(s) 401
Hin1II CATG 5 cut(s) 295, 500, 754, 812, 891
HindIII AAGCTT 1 cut(s) 669
HinfI GANTC 5 cut(s) 363, 518, 658, 724, 869
HpaII CCGG 1 cut(s) 479
HphI GGTGA 3 cut(s) 536, 653, 878
Hpy188I TCNGA 3 cut(s) 97, 399, 413
Hpy188III TCNNGA 2 cut(s) 354, 497
Hpy99I CGWCG 2 cut(s) 70, 137
HpyAV CCTTC 5 cut(s) 346, 387, 577, 755, 806
HpyCH4IV ACGT 3 cut(s) 401, 687, 702
HpyCH4V TGCA 3 cut(s) 17, 792, 818
HpyF10VI GCNNNNNNNGC 1 cut(s) 824
HpyF3I CTNAG 4 cut(s) 284, 566, 623, 782
HpySE526I ACGT 3 cut(s) 401, 687, 702
Hsp92I GRCGYC 1 cut(s) 401
Hsp92II CATG 5 cut(s) 295, 500, 754, 812, 891
Ksp22I TGATCA 1 cut(s) 499
Kzo9I GATC 3 cut(s) 430, 499, 884
LmnI GCTCC 1 cut(s) 307
LweI GCATC 1 cut(s) 203
MaeI CTAG 3 cut(s) 369, 596, 882
MaeII ACGT 3 cut(s) 401, 687, 702
MaeIII GTNAC 2 cut(s) 551, 561
MalI GATC 3 cut(s) 432, 501, 886
MboI GATC 3 cut(s) 430, 499, 884
MboII GAAGA 5 cut(s) 63, 107, 321, 834, 864
MfeI CAATTG 1 cut(s) 56
MflI RGATCY 1 cut(s) 430
MhlI GDGCHC 3 cut(s) 228, 248, 631
MluCI AATT 5 cut(s) 56, 260, 449, 729, 802
MlyI GAGTC 2 cut(s) 652, 733
MnlI CCTC 5 cut(s) 96, 151, 167, 309, 759
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 3 cut(s) 9, 386, 898
MslI CAYNNNNRTG 1 cut(s) 813
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 4 cut(s) 161, 241, 799, 829
MunI CAATTG 1 cut(s) 56
Mva1269I GAATGC 1 cut(s) 829
MvaI CCWGG 4 cut(s) 161, 241, 799, 829
MwoI GCNNNNNNNGC 1 cut(s) 824
NdeII GATC 3 cut(s) 430, 499, 884
NlaIII CATG 5 cut(s) 295, 500, 754, 812, 891
NlaIV GGNNCC 3 cut(s) 245, 410, 588
NmuCI GTSAC 1 cut(s) 561
PagI TCATGA 1 cut(s) 496
PctI GAATGC 1 cut(s) 829
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 3 cut(s) 363, 518, 869
PflMI CCANNNNNTGG 1 cut(s) 834
PleI GAGTC 2 cut(s) 652, 732
PpsI GAGTC 2 cut(s) 652, 732
PshAI GACNNNNGTC 1 cut(s) 854
Psp6I CCWGG 4 cut(s) 159, 239, 797, 827
PspGI CCWGG 4 cut(s) 159, 239, 797, 827
PspN4I GGNNCC 3 cut(s) 245, 410, 588
PspPI GGNCC 2 cut(s) 409, 422
PsuI RGATCY 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 813
SaqAI TTAA 3 cut(s) 9, 386, 898
Sau3AI GATC 3 cut(s) 430, 499, 884
Sau96I GGNCC 2 cut(s) 409, 422
SchI GAGTC 2 cut(s) 652, 733
ScrFI CCNGG 4 cut(s) 161, 241, 799, 829
SduI GDGCHC 3 cut(s) 228, 248, 631
SfaNI GCATC 1 cut(s) 203
SinI GGWCC 2 cut(s) 409, 422
SmiMI CAYNNNNRTG 1 cut(s) 813
SmlI CTYRAG 3 cut(s) 300, 352, 473
SmoI CTYRAG 3 cut(s) 300, 352, 473
Sse9I AATT 5 cut(s) 56, 260, 449, 729, 802
SspI AATATT 1 cut(s) 711
SspMI CTAG 3 cut(s) 369, 596, 882
StyD4I CCNGG 4 cut(s) 159, 239, 797, 827
StyI CCWWGG 1 cut(s) 648
TaiI ACGT 3 cut(s) 404, 690, 705
TasI AATT 5 cut(s) 56, 260, 449, 729, 802
TfiI GAWTC 3 cut(s) 363, 518, 869
Tru1I TTAA 3 cut(s) 9, 386, 898
Tru9I TTAA 3 cut(s) 9, 386, 898
TscAI CASTG 1 cut(s) 214
TseFI GTSAC 1 cut(s) 561
Tsp45I GTSAC 1 cut(s) 561
TspDTI ATGAA 4 cut(s) 166, 280, 825, 864
TspGWI ACGGA 2 cut(s) 95, 217
TspRI CASTG 1 cut(s) 214
Van91I CCANNNNNTGG 1 cut(s) 834
VpaK11BI GGWCC 2 cut(s) 409, 422
XapI RAATTY 1 cut(s) 802
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 3 cut(s) 369, 596, 882
ZraI GACGTC 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.