AT3G06140

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0013528
Physical Location & Seq
Reverse (-)
1856682 .. 1858946
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06140.1

Sequence Viewer

Length: 1080 bp
ATGGGAATCTCCTTTAGCAACAACAACAGAAGACGAGACAACAATAATCGTCGCCACCTCCACCACTATCCTCCGCCGCCGCCTTACTATTACCTCGACCCTCCTCCTCCTCCTCCGCCATTTCCACCGCATTATGACTATAACTACTCCAATTACCATCTCTCTCCGCCTCTACCGCCACAGCCACAGATCAACTCTTGTTCTTACGGCCATTACCATTATCACCCTCAGCCTCCTCAGTATTTCACCACCGCTCAGCCCAATTGGTGGGGCCCAATGATGAGACCGGCGTACTACTGTCCGCCTCAGCCGCAAACGCAACCGCCTAAGCCATACCTGGAGCAACAGAACGCGAAGAAGGTGAGGAATGATGTCAATGTGCATAGAGATACAGTGAGGCTTGAAGTGGACGATCTCGTTCCTGGTCACCATCTAGTTTCCTTCGTCTTCGATGCTCTTTTCGACGGCAGTTTCACTATCACCTTCTTTGCAAAGGAGGAACCAAATTGCACCATTATCCCACAGTTTCCAGAGGTTTATTCACCCACAAGATTCCATTTTCAGAAAGGTCCTGGACAGAAGTTTCTGCAGCCTTCAGGGACAGGAACTGACCTGAGCTTCTTTGTTCTTGATGATCTGTCTAAACCCTTAGAAGAAGATGTGTATCCGCTTGTAATATCAGCTGAGACGATAATCTCTCCAAATTCAATCTCAGAGCAATCATCAGTTCATAAGCAAGTCACACAAGCGGTTCTCGAGAAAGATAATGATGGATCTTTCAAAGTGAAAGTTGTGAAGCAGATTCTTTGGATTGAAGGAGTTCGATATGAACTCCGTGAGTTGTATGGCTCGACCACTCAAGGTGCAGCCTCGGGCTTAGATGAGAGTGGTTCGGGTACAGAATGTGTTATCTGCATGACTGAGGCTAAGGACACGGCCGTGCTGCCTTGCAGACATTTGTGCATGTGTAGCGACTGTGCTAAAGAATTGAGGCTTCAGTCCAACAAATGTCCGATTTGTCGACAACCCATCGAAGAGCTCTTAGAGATCAAAATGAACAGTAGTGATGAACAACACTAG

Protein Analysis

359

Amino Acids

41.0

Weight (kDa)

5.91

Isoelectric Point (pI)

69.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 125 - 278 7.7e-67 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 300 - 346 1.8e-17 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 301 - 341 7.8e-08 Ring finger domain
Prok-RING_4 PF14447 302 - 345 1e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06140
fragaria_vesca FvH4_4g14510
malus_domestica MD13G1183800.v1.1 MD16G1184200.v1.1
prunus_persica Prupe.1G151600_v2.0.a1 Prupe.1G151600_v2.0.a1
pyrus_communis pycom13g15860 pycom16g15470
rosa_chinensis RchiOBHm_Chr4g0418321
rosa_laevigata RLG00000007877
rosa_multiflora Rmu_ssc0000153.1_g000004
rosa_roxburghii Rroxscaffold_5G00361510
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213700 Rh4BG216700 Rh4CG225300 Rh4DG212200
rosa_wichuraiana Rw4G018150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 267
AccBSI CCGCTC 1 cut(s) 254
AccI GTMKAC 1 cut(s) 1021
AccII CGCG 1 cut(s) 353
AclWI GGATC 1 cut(s) 781
AcoI YGGCCR 2 cut(s) 208, 936
AcsI RAATTY 1 cut(s) 703
AcuI CTGAAG 2 cut(s) 579, 980
AfaI GTAC 2 cut(s) 293, 898
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 4 cut(s) 404, 708, 781, 815
AjnI CCWGG 3 cut(s) 336, 421, 571
AleI CACNNNNGTG 1 cut(s) 938
AluBI AGCT 3 cut(s) 618, 683, 1039
AluI AGCT 3 cut(s) 618, 683, 1039
Alw21I GWGCWC 1 cut(s) 1041
Alw26I GTCTC 3 cut(s) 30, 277, 680
AlwI GGATC 1 cut(s) 781
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 2 cut(s) 755, 871
AoxI GGCC 3 cut(s) 208, 271, 936
ApaI GGGCCC 1 cut(s) 275
ApeKI GCWGC 3 cut(s) 589, 866, 943
ApoI RAATTY 1 cut(s) 703
Asp700I GAANNNNTTC 1 cut(s) 819
AspS9I GGNCC 3 cut(s) 271, 272, 569
AsuHPI GGTGA 6 cut(s) 215, 238, 373, 419, 472, 534
AvaI CYCGRG 2 cut(s) 755, 871
AvaII GGWCC 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 275
BanII GRGCYC 2 cut(s) 275, 1041
BbsI GAAGAC 2 cut(s) 37, 439
Bbv12I GWGCWC 1 cut(s) 1041
BbvCI CCTCAGC 2 cut(s) 228, 306
BbvI GCAGC 3 cut(s) 601, 878, 930
BccI CCATC 4 cut(s) 165, 438, 764, 1037
BceAI ACGGC 4 cut(s) 223, 481, 923, 951
BciT130I CCWGG 3 cut(s) 338, 423, 573
BciVI GTATCC 1 cut(s) 675
BcoDI GTCTC 3 cut(s) 30, 277, 680
BfaI CTAG 2 cut(s) 434, 1078
BfmI CTRYAG 1 cut(s) 587
BfuI GTATCC 1 cut(s) 675
BisI GCNGC 6 cut(s) 77, 80, 311, 590, 867, 944
BlpI GCTNAGC 1 cut(s) 255
BlsI GCNGC 6 cut(s) 78, 81, 312, 591, 868, 945
Bme1390I CCNGG 3 cut(s) 338, 423, 573
Bme18I GGWCC 1 cut(s) 569
BmeT110I CYCGRG 2 cut(s) 755, 871
BmgT120I GGNCC 3 cut(s) 271, 272, 569
BmiI GGNNCC 3 cut(s) 272, 273, 501
BmrFI CCNGG 3 cut(s) 338, 423, 573
BmsI GCATC 1 cut(s) 442
BpiI GAAGAC 2 cut(s) 37, 439
BpmI CTGGAG 1 cut(s) 359
Bpu10I CCTNAGC 5 cut(s) 228, 306, 327, 614, 927
Bpu1102I GCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 843
BsaBI GATNNNNATC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 277
BsaJI CCNNGG 1 cut(s) 870
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 286
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 3 cut(s) 338, 423, 573
BseDI CCNNGG 1 cut(s) 870
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMII CTCAG 8 cut(s) 242, 251, 269, 320, 605, 675, 726, 912
BseRI GAGGAG 5 cut(s) 93, 96, 99, 102, 225
BseSI GKGCMC 1 cut(s) 275
BseX3I CGGCCG 1 cut(s) 936
BseXI GCAGC 3 cut(s) 601, 878, 930
BsgI GTGCAG 1 cut(s) 885
Bsh1236I CGCG 1 cut(s) 353
Bsh1285I CGRYCG 1 cut(s) 939
BshFI GGCC 3 cut(s) 210, 273, 938
BsiEI CGRYCG 1 cut(s) 939
BsiHKAI GWGCWC 1 cut(s) 1041
BsiHKCI CYCGRG 2 cut(s) 755, 871
BsiSI CCGG 1 cut(s) 287
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 3 cut(s) 30, 277, 680
BsmBI CGTCTC 1 cut(s) 680
BsmFI GGGAC 1 cut(s) 613
BsnI GGCC 3 cut(s) 210, 273, 938
Bso31I GGTCTC 1 cut(s) 277
BsoBI CYCGRG 2 cut(s) 755, 871
Bsp120I GGGCCC 1 cut(s) 271
Bsp1286I GDGCHC 2 cut(s) 275, 1041
Bsp143I GATC 5 cut(s) 189, 412, 634, 773, 1047
Bsp1720I GCTNAGC 1 cut(s) 255
BspANI GGCC 3 cut(s) 210, 273, 938
BspCNI CTCAG 8 cut(s) 241, 250, 268, 319, 606, 676, 725, 913
BspFNI CGCG 1 cut(s) 353
BspLI GGNNCC 3 cut(s) 272, 273, 501
BspMAI CTGCAG 1 cut(s) 591
BspPI GGATC 1 cut(s) 781
BspQI GCTCTTC 1 cut(s) 1029
BspTNI GGTCTC 1 cut(s) 277
BsrBI CCGCTC 1 cut(s) 254
BsrFI RCCGGY 1 cut(s) 286
BssAI RCCGGY 1 cut(s) 286
BssECI CCNNGG 1 cut(s) 870
BssMI GATC 5 cut(s) 189, 412, 634, 773, 1047
Bst2UI CCWGG 3 cut(s) 338, 423, 573
Bst4CI ACNGT 5 cut(s) 299, 394, 525, 977, 1061
Bst6I CTCTTC 1 cut(s) 1029
BstEII GGTNACC 1 cut(s) 425
BstFNI CGCG 1 cut(s) 353
BstKTI GATC 5 cut(s) 192, 415, 637, 776, 1050
BstMAI GTCTC 3 cut(s) 30, 277, 680
BstMBI GATC 5 cut(s) 189, 412, 634, 773, 1047
BstMCI CGRYCG 1 cut(s) 939
BstMWI GCNNNNNNNGC 4 cut(s) 175, 310, 316, 969
BstNI CCWGG 3 cut(s) 338, 423, 573
BstNSI RCATGY 1 cut(s) 967
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 3 cut(s) 336, 421, 571
BstSFI CTRYAG 1 cut(s) 587
BstSLI GKGCMC 1 cut(s) 275
BstUI CGCG 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 601, 878, 930
BstV2I GAAGAC 2 cut(s) 37, 439
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BstZI CGGCCG 1 cut(s) 936
BsuI GTATCC 1 cut(s) 675
BsuRI GGCC 3 cut(s) 210, 273, 938
BtsIMutI CAGTG 1 cut(s) 399
CaiI CAGNNNCTG 1 cut(s) 608
Cfr10I RCCGGY 1 cut(s) 286
Cfr13I GGNCC 3 cut(s) 271, 272, 569
Csp6I GTAC 2 cut(s) 292, 897
CviAII CATG 2 cut(s) 916, 964
CviQI GTAC 2 cut(s) 292, 897
DpnI GATC 5 cut(s) 191, 414, 636, 775, 1049
DpnII GATC 5 cut(s) 189, 412, 634, 773, 1047
EaeI YGGCCR 2 cut(s) 208, 936
EagI CGGCCG 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 1029
EarI CTCTTC 1 cut(s) 1029
EciI GGCGGA 4 cut(s) 63, 105, 156, 291
Ecl136II GAGCTC 1 cut(s) 1039
EclXI CGGCCG 1 cut(s) 936
Eco24I GRGCYC 2 cut(s) 275, 1041
Eco31I GGTCTC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 569
Eco52I CGGCCG 1 cut(s) 936
Eco53kI GAGCTC 1 cut(s) 1039
Eco57I CTGAAG 2 cut(s) 579, 980
Eco88I CYCGRG 2 cut(s) 755, 871
Eco91I GGTNACC 1 cut(s) 425
EcoICRI GAGCTC 1 cut(s) 1039
EcoO109I RGGNCCY 2 cut(s) 271, 569
EcoO65I GGTNACC 1 cut(s) 425
EcoRII CCWGG 3 cut(s) 336, 421, 571
EcoT38I GRGCYC 2 cut(s) 275, 1041
Esp3I CGTCTC 1 cut(s) 680
FaeI CATG 2 cut(s) 919, 967
FaiI YATR 9 cut(s) 135, 141, 334, 384, 732, 828, 846, 917, 965
FaqI GGGAC 1 cut(s) 613
FatI CATG 2 cut(s) 915, 963
FblI GTMKAC 1 cut(s) 1021
Fnu4HI GCNGC 6 cut(s) 77, 80, 311, 590, 867, 944
FriOI GRGCYC 2 cut(s) 275, 1041
Fsp4HI GCNGC 6 cut(s) 77, 80, 311, 590, 867, 944
FspBI CTAG 2 cut(s) 434, 1078
GluI GCNGC 6 cut(s) 77, 80, 311, 590, 867, 944
GsuI CTGGAG 1 cut(s) 359
HaeIII GGCC 3 cut(s) 210, 273, 938
HapII CCGG 1 cut(s) 287
Hin1II CATG 2 cut(s) 919, 967
HincII GTYRAC 1 cut(s) 1022
HindII GTYRAC 1 cut(s) 1022
HinfI GANTC 3 cut(s) 6, 552, 802
HpaII CCGG 1 cut(s) 287
HphI GGTGA 6 cut(s) 215, 238, 373, 419, 472, 534
Hpy166II GTNNAC 2 cut(s) 409, 1022
Hpy188I TCNGA 3 cut(s) 564, 715, 1014
Hpy188III TCNNGA 4 cut(s) 530, 629, 755, 757
Hpy8I GTNNAC 2 cut(s) 409, 1022
Hpy99I CGWCG 2 cut(s) 54, 467
HpyAV CCTTC 5 cut(s) 352, 451, 493, 603, 809
HpyCH4III ACNGT 5 cut(s) 299, 394, 525, 977, 1061
HpyCH4V TGCA 8 cut(s) 382, 491, 510, 589, 866, 915, 951, 963
HpyF10VI GCNNNNNNNGC 4 cut(s) 175, 310, 316, 969
Hsp92II CATG 2 cut(s) 919, 967
Kzo9I GATC 5 cut(s) 189, 412, 634, 773, 1047
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 3 cut(s) 601, 878, 930
LweI GCATC 1 cut(s) 442
MaeI CTAG 2 cut(s) 434, 1078
MaeIII GTNAC 2 cut(s) 425, 739
MalI GATC 5 cut(s) 191, 414, 636, 775, 1049
MbiI CCGCTC 1 cut(s) 254
MboI GATC 5 cut(s) 189, 412, 634, 773, 1047
MboII GAAGA 6 cut(s) 42, 367, 439, 665, 668, 1046
MfeI CAATTG 1 cut(s) 262
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 2 cut(s) 275, 1041
MluCI AATT 5 cut(s) 151, 262, 505, 703, 986
MmeI TCCRAC 1 cut(s) 1026
MroXI GAANNNNTTC 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 938
MspA1I CMGCKG 1 cut(s) 683
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 3 cut(s) 338, 423, 573
MunI CAATTG 1 cut(s) 262
MvaI CCWGG 3 cut(s) 338, 423, 573
MvnI CGCG 1 cut(s) 353
MwoI GCNNNNNNNGC 4 cut(s) 175, 310, 316, 969
NdeII GATC 5 cut(s) 189, 412, 634, 773, 1047
NlaIII CATG 2 cut(s) 919, 967
NlaIV GGNNCC 3 cut(s) 272, 273, 501
NmuCI GTSAC 2 cut(s) 425, 739
NspI RCATGY 1 cut(s) 967
OliI CACNNNNGTG 1 cut(s) 938
PaeR7I CTCGAG 1 cut(s) 755
PciSI GCTCTTC 1 cut(s) 1029
PdmI GAANNNNTTC 1 cut(s) 819
PfeI GAWTC 3 cut(s) 6, 552, 802
PflMI CCANNNNNTGG 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 6 cut(s) 78, 81, 312, 591, 868, 945
PpuMI RGGWCCY 1 cut(s) 569
Psp124BI GAGCTC 1 cut(s) 1041
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 3 cut(s) 336, 421, 571
PspEI GGTNACC 1 cut(s) 425
PspGI CCWGG 3 cut(s) 336, 421, 571
PspN4I GGNNCC 3 cut(s) 272, 273, 501
PspOMI GGGCCC 1 cut(s) 271
PspPI GGNCC 3 cut(s) 271, 272, 569
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 1 cut(s) 591
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 773
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 2 cut(s) 293, 898
RsaNI GTAC 2 cut(s) 292, 897
RseI CAYNNNNRTG 1 cut(s) 938
SacI GAGCTC 1 cut(s) 1041
SalI GTCGAC 1 cut(s) 1020
SapI GCTCTTC 1 cut(s) 1029
SatI GCNGC 6 cut(s) 77, 80, 311, 590, 867, 944
Sau3AI GATC 5 cut(s) 189, 412, 634, 773, 1047
Sau96I GGNCC 3 cut(s) 271, 272, 569
ScrFI CCNGG 3 cut(s) 338, 423, 573
SduI GDGCHC 2 cut(s) 275, 1041
SfaNI GCATC 1 cut(s) 442
SfcI CTRYAG 1 cut(s) 587
Sfr274I CTCGAG 1 cut(s) 755
SinI GGWCC 1 cut(s) 569
SlaI CTCGAG 1 cut(s) 755
SmiMI CAYNNNNRTG 1 cut(s) 938
SmlI CTYRAG 2 cut(s) 755, 858
SmoI CTYRAG 2 cut(s) 755, 858
Sse9I AATT 5 cut(s) 151, 262, 505, 703, 986
SspMI CTAG 2 cut(s) 434, 1078
SstI GAGCTC 1 cut(s) 1041
StyD4I CCNGG 3 cut(s) 336, 421, 571
TaaI ACNGT 5 cut(s) 299, 394, 525, 977, 1061
TaqI TCGA 8 cut(s) 96, 450, 462, 756, 823, 851, 1021, 1032
TasI AATT 5 cut(s) 151, 262, 505, 703, 986
TauI GCSGC 3 cut(s) 79, 82, 313
TfiI GAWTC 3 cut(s) 6, 552, 802
TscAI CASTG 1 cut(s) 399
TseFI GTSAC 2 cut(s) 425, 739
TseI GCWGC 3 cut(s) 589, 866, 943
Tsp45I GTSAC 2 cut(s) 425, 739
TspDTI ATGAA 3 cut(s) 719, 843, 1070
TspGWI ACGGA 1 cut(s) 824
TspRI CASTG 1 cut(s) 399
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 1 cut(s) 703
XceI RCATGY 1 cut(s) 967
XhoI CTCGAG 1 cut(s) 755
XmiI GTMKAC 1 cut(s) 1021
XmnI GAANNNNTTC 1 cut(s) 819
XspI CTAG 2 cut(s) 434, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.