Rw4G018150

E3 ubiquitin-protein ligase LUL4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013528
Physical Location & Seq
Reverse (-)
40376577 .. 40380836
4260 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018150.1

Sequence Viewer

Length: 1209 bp
ATGGGGATTTCTTTCAGCAGCAACCGAAGACGTAACAACTACTACCAACACCCGCCGCCGCCTCAGCCCCTCTACCTCTCCAATCCTCACTACTACCCCTCCGACCCACCCTCTTTCTCTCCGCCTCCGCCGCCCCCGCCACCTCCTCCGCCGCCGGTGACTTCTTTTTCTTCCTCCTCCTCCTCCTCTTCTTCTTCCGCCGCCTACCCAGAACCCAATTCCGCCACCCCTTACTCCGCGCCGCCGCCCAGGGCTCCATACGCCTACCCTCTTCCGCCGCCGCCGCAGCAGTCGCATTACTGCTACGGTCAGTACAATTCTTGCAACCACGCGAATCCGATGATGGGTCGGTTCAGTTACAACCACAATTACCAGCCTTACTATGGTAATCAGGCGAGTGGGTGGCCGGCGATGCGGCCTCCGGTGGCCCCCCTTTATCCTCCGCCGCCGTACGTGGAGCACCAGAGTGCCAAGAAGGTGAAGAATGACGTCAACGTGCGTAAGGACACTTTGAGGCTGGAGATTGACGAGCAGAACCCTGATCAGCATTTGGTCTCGTTCGTTTTCGATGCCTTGTATGATGGGAGCATTACTATTCTCTACTTTGCCAAGGAAGAGGCAGATTGTACATTTGTTCCACTATTTCCTGAAGCATTTACACCGGTGCAAGTCCCTTTTCAGAAAGGAGTAGGCCAGAAATTTTGTCAGCCCTCAGGAACAGGCATCGATTTAGGCTTCTTCGAGTTGGATGATCTGTCAAAACCCTCACCTGGAGAAGATGTATTCCCACTTGTAATATCTGCGGAAACACGTACGCCGCCTGATTCAATCGACCATATTGGTGAGCCTGTCCAAGATGCATCTCCTCGTATGCAGATCACTCAAGCTGTGCTCGAAAAAAATAATGGTGACCCTTTCCAAGTGAAAGTAATTAAGCAGATACTGTGGATTGATGAAGTTCGCTATGAACTACGTGAGATATATGGAATTGCAACCTCAGCAGTGGAAGGCTTTGATGACAATGACCCTGGGAAGGAGTGTGTAATATGCATGACCGAACCGAAGGACACTGCTGTATTACCTTGTCGACATATGTGTATGTGCAGTGACTGTGCAAAAGAATTGAGGATTCAATCCAATAAATGCCCCATTTGCCGGCAACCCATTGAAGAACTTATAGAGATAAAGATGAATAAAAGCCAGCAATGA

Protein Analysis

402

Amino Acids

44.94

Weight (kDa)

5.24

Isoelectric Point (pI)

71.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 164 - 324 1.6e-70 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 345 - 391 8.6e-18 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 346 - 386 1.6e-07 Ring finger domain
Prok-RING_4 PF14447 347 - 390 2.3e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06140
fragaria_vesca FvH4_4g14510
malus_domestica MD13G1183800.v1.1 MD16G1184200.v1.1
prunus_persica Prupe.1G151600_v2.0.a1 Prupe.1G151600_v2.0.a1
pyrus_communis pycom13g15860 pycom16g15470
rosa_chinensis RchiOBHm_Chr4g0418321
rosa_laevigata RLG00000007877
rosa_multiflora Rmu_ssc0000153.1_g000004
rosa_roxburghii Rroxscaffold_5G00361510
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213700 Rh4BG216700 Rh4CG225300 Rh4DG212200
rosa_wichuraiana Rw4G018150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 492
AccI GTMKAC 1 cut(s) 1087
AccII CGCG 2 cut(s) 239, 332
AcoI YGGCCR 1 cut(s) 404
AcsI RAATTY 1 cut(s) 698
AcuI CTGAAG 1 cut(s) 669
AcyI GRCGYC 1 cut(s) 489
AfaI GTAC 4 cut(s) 314, 452, 628, 814
AfiI CCNNNNNNNGG 5 cut(s) 344, 383, 770, 1033, 1155
AflIII ACRYGT 1 cut(s) 809
AgeI ACCGGT 1 cut(s) 661
AgsI TTSAA 3 cut(s) 828, 1133, 1169
AjnI CCWGG 3 cut(s) 248, 769, 1027
AleI CACNNNNGTG 1 cut(s) 465
AluBI AGCT 1 cut(s) 887
AluI AGCT 1 cut(s) 887
Alw21I GWGCWC 2 cut(s) 462, 894
Alw26I GTCTC 1 cut(s) 559
AlwNI CAGNNNCTG 2 cut(s) 943, 1110
AoxI GGCC 4 cut(s) 404, 416, 426, 691
ApeKI GCWGC 2 cut(s) 18, 286
ApoI RAATTY 1 cut(s) 698
AsiGI ACCGGT 1 cut(s) 661
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 427
AsuHPI GGTGA 5 cut(s) 169, 490, 759, 854, 920
AxyI CCTNAGG 1 cut(s) 712
BanII GRGCYC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 462, 894
BbvCI CCTCAGC 2 cut(s) 63, 997
BbvI GCAGC 2 cut(s) 30, 298
BccI CCATC 2 cut(s) 337, 575
BceAI ACGGC 1 cut(s) 433
BciT130I CCWGG 3 cut(s) 250, 771, 1029
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 559
Bme1390I CCNGG 3 cut(s) 250, 771, 1029
BmgT120I GGNCC 1 cut(s) 427
BmiI GGNNCC 2 cut(s) 255, 429
BmrFI CCNGG 3 cut(s) 250, 771, 1029
BmsI GCATC 5 cut(s) 402, 559, 732, 847, 869
BpiI GAAGAC 1 cut(s) 34
BpmI CTGGAG 2 cut(s) 539, 792
Bpu10I CCTNAGC 2 cut(s) 63, 997
BpuEI CTTGAG 1 cut(s) 867
Bsa29I ATCGAT 1 cut(s) 726
BsaAI YACGTR 3 cut(s) 454, 812, 974
BsaHI GRCGYC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 5 cut(s) 248, 249, 609, 1027, 1028
BsaWI WCCGGW 2 cut(s) 421, 661
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 765, 795
Bsc4I CCNNNNNNNGG 5 cut(s) 344, 383, 770, 1033, 1155
Bse118I RCCGGY 4 cut(s) 154, 406, 661, 1155
Bse21I CCTNAGG 1 cut(s) 712
BseBI CCWGG 3 cut(s) 250, 771, 1029
BseCI ATCGAT 1 cut(s) 726
BseDI CCNNGG 5 cut(s) 248, 249, 609, 1027, 1028
BseGI GGATG 1 cut(s) 754
BseLI CCNNNNNNNGG 5 cut(s) 344, 383, 770, 1033, 1155
BseMII CTCAG 3 cut(s) 77, 726, 1011
BseRI GAGGAG 6 cut(s) 135, 166, 169, 172, 175, 855
BseXI GCAGC 2 cut(s) 30, 298
BsgI GTGCAG 1 cut(s) 1123
Bsh1236I CGCG 2 cut(s) 239, 332
BshFI GGCC 4 cut(s) 406, 418, 428, 693
BshTI ACCGGT 1 cut(s) 661
BshVI ATCGAT 1 cut(s) 726
BsiHKAI GWGCWC 2 cut(s) 462, 894
BsiSI CCGG 5 cut(s) 155, 407, 422, 662, 1156
BsiWI CGTACG 2 cut(s) 450, 812
BslFI GGGAC 1 cut(s) 656
BslI CCNNNNNNNGG 5 cut(s) 344, 383, 770, 1033, 1155
BsmAI GTCTC 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 656
BsnI GGCC 4 cut(s) 406, 418, 428, 693
Bso31I GGTCTC 1 cut(s) 559
Bsp1286I GDGCHC 3 cut(s) 256, 462, 894
Bsp1407I TGTACA 1 cut(s) 626
Bsp143I GATC 3 cut(s) 541, 751, 876
BspANI GGCC 4 cut(s) 406, 418, 428, 693
BspCNI CTCAG 3 cut(s) 76, 725, 1010
BspDI ATCGAT 1 cut(s) 726
BspFNI CGCG 2 cut(s) 239, 332
BspLI GGNNCC 2 cut(s) 255, 429
BspTNI GGTCTC 1 cut(s) 559
BsrFI RCCGGY 4 cut(s) 154, 406, 661, 1155
BsrGI TGTACA 1 cut(s) 626
BssAI RCCGGY 4 cut(s) 154, 406, 661, 1155
BssECI CCNNGG 5 cut(s) 248, 249, 609, 1027, 1028
BssMI GATC 3 cut(s) 541, 751, 876
BssNI GRCGYC 1 cut(s) 489
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 3 cut(s) 250, 771, 1029
Bst4CI ACNGT 3 cut(s) 308, 945, 1112
Bst6I CTCTTC 3 cut(s) 193, 276, 609
BstACI GRCGYC 1 cut(s) 489
BstAUI TGTACA 1 cut(s) 626
BstBAI YACGTR 3 cut(s) 454, 812, 974
BstC8I GCNNGC 3 cut(s) 408, 1157, 1202
BstDEI CTNAG 3 cut(s) 63, 712, 997
BstEII GGTNACC 1 cut(s) 908
BstF5I GGATG 1 cut(s) 754
BstFNI CGCG 2 cut(s) 239, 332
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 3 cut(s) 544, 754, 879
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 3 cut(s) 541, 751, 876
BstNI CCWGG 3 cut(s) 250, 771, 1029
BstPI GGTNACC 1 cut(s) 908
BstSCI CCNGG 3 cut(s) 248, 769, 1027
BstUI CGCG 2 cut(s) 239, 332
BstV1I GCAGC 2 cut(s) 30, 298
BstV2I GAAGAC 1 cut(s) 34
Bsu15I ATCGAT 1 cut(s) 726
Bsu36I CCTNAGG 1 cut(s) 712
BsuRI GGCC 4 cut(s) 406, 418, 428, 693
BsuTUI ATCGAT 1 cut(s) 726
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 1 cut(s) 754
BtsI GCAGTG 3 cut(s) 1008, 1068, 1111
BtsIMutI CAGTG 3 cut(s) 1008, 1068, 1111
Cac8I GCNNGC 3 cut(s) 408, 1157, 1202
CaiI CAGNNNCTG 2 cut(s) 943, 1110
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 4 cut(s) 154, 406, 661, 1155
Cfr13I GGNCC 1 cut(s) 427
ClaI ATCGAT 1 cut(s) 726
Csp6I GTAC 4 cut(s) 313, 451, 627, 813
CspAI ACCGGT 1 cut(s) 661
CviAII CATG 1 cut(s) 1051
CviQI GTAC 4 cut(s) 313, 451, 627, 813
DdeI CTNAG 3 cut(s) 63, 712, 997
DpnI GATC 3 cut(s) 543, 753, 878
DpnII GATC 3 cut(s) 541, 751, 876
EaeI YGGCCR 1 cut(s) 404
Eam1104I CTCTTC 3 cut(s) 193, 276, 609
EarI CTCTTC 3 cut(s) 193, 276, 609
EciI GGCGGA 7 cut(s) 111, 117, 138, 187, 211, 264, 432
Eco130I CCWWGG 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 256
Eco31I GGTCTC 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 669
Eco81I CCTNAGG 1 cut(s) 712
Eco91I GGTNACC 1 cut(s) 908
EcoO65I GGTNACC 1 cut(s) 908
EcoRII CCWGG 3 cut(s) 248, 769, 1027
EcoT14I CCWWGG 1 cut(s) 609
EcoT22I ATGCAT 2 cut(s) 862, 1052
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 1 cut(s) 1054
FaqI GGGAC 1 cut(s) 656
FatI CATG 1 cut(s) 1050
FauI CCCGC 2 cut(s) 60, 144
FauNDI CATATG 1 cut(s) 1092
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 1087
FokI GGATG 1 cut(s) 761
FriOI GRGCYC 1 cut(s) 256
GlaI GCGC 1 cut(s) 240
GsuI CTGGAG 2 cut(s) 539, 792
HaeIII GGCC 4 cut(s) 406, 418, 428, 693
HapII CCGG 5 cut(s) 155, 407, 422, 662, 1156
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 489
Hin1II CATG 1 cut(s) 1054
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 2 cut(s) 493, 1088
HindII GTYRAC 2 cut(s) 493, 1088
HinfI GANTC 3 cut(s) 334, 824, 1129
HpaII CCGG 5 cut(s) 155, 407, 422, 662, 1156
HphI GGTGA 5 cut(s) 169, 490, 759, 854, 920
Hpy166II GTNNAC 2 cut(s) 493, 1088
Hpy188I TCNGA 3 cut(s) 103, 339, 681
Hpy188III TCNNGA 2 cut(s) 647, 714
Hpy8I GTNNAC 2 cut(s) 493, 1088
HpyAV CCTTC 4 cut(s) 469, 1001, 1027, 1057
HpyCH4III ACNGT 3 cut(s) 308, 945, 1112
HpyCH4IV ACGT 6 cut(s) 31, 453, 489, 495, 811, 973
HpyCH4V TGCA 8 cut(s) 324, 667, 860, 874, 992, 1050, 1104, 1115
HpyF3I CTNAG 3 cut(s) 63, 712, 997
HpySE526I ACGT 6 cut(s) 31, 453, 489, 495, 811, 973
Hsp92I GRCGYC 1 cut(s) 489
Hsp92II CATG 1 cut(s) 1054
HspAI GCGC 1 cut(s) 239
KroI GCCGGC 2 cut(s) 406, 1155
KroNI GCCGGC 2 cut(s) 408, 1157
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 3 cut(s) 541, 751, 876
LmnI GCTCC 3 cut(s) 259, 457, 585
Lsp1109I GCAGC 2 cut(s) 30, 298
LweI GCATC 5 cut(s) 402, 559, 732, 847, 869
MaeII ACGT 6 cut(s) 31, 453, 489, 495, 811, 973
MaeIII GTNAC 5 cut(s) 32, 157, 356, 908, 1106
MalI GATC 3 cut(s) 543, 753, 878
MboI GATC 3 cut(s) 541, 751, 876
MhlI GDGCHC 3 cut(s) 256, 462, 894
MluCI AATT 7 cut(s) 217, 316, 367, 698, 930, 987, 1121
MmeI TCCRAC 2 cut(s) 126, 726
Mph1103I ATGCAT 2 cut(s) 862, 1052
MroNI GCCGGC 2 cut(s) 406, 1155
MseI TTAA 1 cut(s) 933
MslI CAYNNNNRTG 2 cut(s) 465, 840
MspI CCGG 5 cut(s) 155, 407, 422, 662, 1156
MspR9I CCNGG 3 cut(s) 250, 771, 1029
MvaI CCWGG 3 cut(s) 250, 771, 1029
MvnI CGCG 2 cut(s) 239, 332
NaeI GCCGGC 2 cut(s) 408, 1157
NdeI CATATG 1 cut(s) 1092
NdeII GATC 3 cut(s) 541, 751, 876
NgoMIV GCCGGC 2 cut(s) 406, 1155
NlaIII CATG 1 cut(s) 1054
NlaIV GGNNCC 2 cut(s) 255, 429
NmuCI GTSAC 3 cut(s) 157, 908, 1106
NsiI ATGCAT 2 cut(s) 862, 1052
OliI CACNNNNGTG 1 cut(s) 465
PasI CCCWGGG 2 cut(s) 249, 1028
PdiI GCCGGC 2 cut(s) 408, 1157
PfeI GAWTC 3 cut(s) 334, 824, 1129
Pfl23II CGTACG 2 cut(s) 450, 812
PinAI ACCGGT 1 cut(s) 661
Ppu21I YACGTR 3 cut(s) 454, 812, 974
Psp6I CCWGG 3 cut(s) 248, 769, 1027
PspEI GGTNACC 1 cut(s) 908
PspGI CCWGG 3 cut(s) 248, 769, 1027
PspLI CGTACG 2 cut(s) 450, 812
PspN4I GGNNCC 2 cut(s) 255, 429
PspPI GGNCC 1 cut(s) 427
PstNI CAGNNNCTG 2 cut(s) 943, 1110
RsaI GTAC 4 cut(s) 314, 452, 628, 814
RsaNI GTAC 4 cut(s) 313, 451, 627, 813
RseI CAYNNNNRTG 2 cut(s) 465, 840
SalI GTCGAC 1 cut(s) 1086
SaqAI TTAA 1 cut(s) 933
Sau3AI GATC 3 cut(s) 541, 751, 876
Sau96I GGNCC 1 cut(s) 427
ScrFI CCNGG 3 cut(s) 250, 771, 1029
SduI GDGCHC 3 cut(s) 256, 462, 894
SfaNI GCATC 5 cut(s) 402, 559, 732, 847, 869
SgrAI CRCCGGYG 2 cut(s) 154, 661
SmiMI CAYNNNNRTG 2 cut(s) 465, 840
SmlI CTYRAG 1 cut(s) 882
SmoI CTYRAG 1 cut(s) 882
Sse9I AATT 7 cut(s) 217, 316, 367, 698, 930, 987, 1121
StyD4I CCNGG 3 cut(s) 248, 769, 1027
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 3 cut(s) 308, 945, 1112
TaiI ACGT 6 cut(s) 34, 456, 492, 498, 814, 976
TaqI TCGA 6 cut(s) 567, 726, 741, 831, 894, 1087
TaqII GACCGA 1 cut(s) 1070
TasI AATT 7 cut(s) 217, 316, 367, 698, 930, 987, 1121
TatI WGTACW 2 cut(s) 312, 626
TfiI GAWTC 3 cut(s) 334, 824, 1129
Tru1I TTAA 1 cut(s) 933
Tru9I TTAA 1 cut(s) 933
TscAI CASTG 3 cut(s) 1008, 1075, 1111
TseFI GTSAC 3 cut(s) 157, 908, 1106
TseI GCWGC 2 cut(s) 18, 286
Tsp45I GTSAC 3 cut(s) 157, 908, 1106
TspDTI ATGAA 3 cut(s) 969, 981, 1205
TspRI CASTG 3 cut(s) 1008, 1075, 1111
XapI RAATTY 1 cut(s) 698
XcmI CCANNNNNNNNNTGG 1 cut(s) 380
XmiI GTMKAC 1 cut(s) 1087
ZraI GACGTC 1 cut(s) 490
Zsp2I ATGCAT 2 cut(s) 862, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.