MD16G1184200.v1.1

E3 ubiquitin-protein ligase LUL4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013528
Physical Location & Seq
Forward (+)
15819745 .. 15823979
4235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1184200.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGGGGATTTCATGGAGCACCAACAGAAGAAGGAACAACTATTACCAAAACCCACCACCGCCTCCGCCGCCACCGCCGCCACGTCAACCCCATTACATCTCAAGTTCTCATCCTTATTACTCATCGGACCATGCCTCTCTTTCTCCACCTCCACCGCCACCTCCTCCTCCTCCTCCACCGCCACCTGGCCCTTTGGTTTCAGCTTCTTCTTCCTCCTCTTTTTCATTTTCTTCTAATAACAACTACCCATATGCCAATTCCGCCACACCTTACGCCCCACAAGGACCGCAACCCAGAGCTCCGTACCCCACACACCCTCTCCCTCTCCTGCCTCCTCCTCCTTCTCCGCCGCAAACCCATTACTGCTACGGCCACTACAACTCCTGCAACCATGGCAATTCCATGATGGGGCGCTTCAGTTACAATTACAATTACAATTACCAGCAGCCTTATAATTATGGTGCTACTCAGAGTAATGGGTGGCCCGCAATTCGGCCTCCTCCGCCGCCTCCGCCACCTTACGTTGAGCATCAGAACGCCAAGAAGGTTAAGAACGATGTCAACGTGCATAAGGACACGTTGAGGCTTGAGATTGATGACCTGAACCCTGATCAGCATTTGGTGTCTTTCGTTTTCGATGCCATGTATGATGGGAGCATTACGGTCTTCTACTTTGCTAAGGAAGAGCCAGACTGTAGGTTTGTTCCACTATTTCCTGAAGCATTTATGCCAGTGAGAGTCCCCTTTCAGAAGGGTCTTGGCCAAAAATTTTGTCAGCCCTCAGGAACAGGAATTGATTTAGGCTTCTTTGAGTTGGATGATCTTTCAAAACCATCACCAGGAGAAGATGTATTTCCGCTTGTAATATCTGCTGAAACATGTCTGCCACCTGATTCAACAGATGAGCATATTGTTGGGCCTACCCCATATACATCCCCTCATATGCAGATTACTCAAGCTGTGCTGGAAAAGAACAATGGTGACCATTTCCAAGTGAAAGTAATTAGGCAGATACTGTGGATTGATGAAGTTCGGTATGAGCTACGTGAGATATATGGAATAGGAAGCTCGACAGTGGAAAGCTTTGATGACACTGAACCGGGGAAGGAATGCGTGATATGCATGACTGAACCTAAGGACACTGCTGTGTTGCCTTGTCGACATATGTGTATGTGCAGTGACTGTGCAAAAGAATTGCGGGTTCAATCCAATAAATGCCCCATCTGCCGTCAACCCATTGAGGAACTTATAGAGATCAAGATAAATAAAAGTGAGCAATGA

Protein Analysis

427

Amino Acids

47.78

Weight (kDa)

5.68

Isoelectric Point (pI)

70.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNF157-like_N PF26192 187 - 348 1.6e-70 RNF157-like N-terminal domain
zf-C3HC4_3 PF13920 369 - 415 9.3e-18 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 370 - 410 9.1e-08 Ring finger domain
Prok-RING_4 PF14447 371 - 414 2.5e-06 Prokaryotic RING finger family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013528)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G06140
fragaria_vesca FvH4_4g14510
malus_domestica MD13G1183800.v1.1 MD16G1184200.v1.1
prunus_persica Prupe.1G151600_v2.0.a1 Prupe.1G151600_v2.0.a1
pyrus_communis pycom13g15860 pycom16g15470
rosa_chinensis RchiOBHm_Chr4g0418321
rosa_laevigata RLG00000007877
rosa_multiflora Rmu_ssc0000153.1_g000004
rosa_roxburghii Rroxscaffold_5G00361510
rosa_rugosa Rorug04G0152200
rosa_samantha Rh4AG213700 Rh4BG216700 Rh4CG225300 Rh4DG212200
rosa_wichuraiana Rw4G018150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 453
AccI GTMKAC 1 cut(s) 1159
AcoI YGGCCR 2 cut(s) 370, 760
AcsI RAATTY 1 cut(s) 767
AcuI CTGAAG 2 cut(s) 400, 738
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 4 cut(s) 185, 408, 492, 839
AflIII ACRYGT 2 cut(s) 576, 878
AgsI TTSAA 3 cut(s) 828, 897, 1205
AjiI CACGTC 1 cut(s) 83
AjnI CCWGG 2 cut(s) 184, 838
AleI CACNNNNGTG 1 cut(s) 1145
AluBI AGCT 6 cut(s) 203, 299, 959, 1042, 1068, 1083
AluI AGCT 6 cut(s) 203, 299, 959, 1042, 1068, 1083
Alw21I GWGCWC 2 cut(s) 20, 301
AlwNI CAGNNNCTG 2 cut(s) 1015, 1182
AoxI GGCC 6 cut(s) 187, 370, 482, 494, 760, 917
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 1 cut(s) 767
ArsI GACNNNNNNTTYG 4 cut(s) 683, 715, 757, 789
AspLEI GCGC 1 cut(s) 414
AspS9I GGNCC 5 cut(s) 127, 188, 284, 483, 917
AsuC2I CCSGG 1 cut(s) 1101
AsuHPI GGTGA 2 cut(s) 828, 992
AvaII GGWCC 2 cut(s) 127, 284
AxyI CCTNAGG 2 cut(s) 781, 1134
BalI TGGCCA 1 cut(s) 762
BanII GRGCYC 1 cut(s) 301
BbsI GAAGAC 1 cut(s) 658
Bbv12I GWGCWC 2 cut(s) 20, 301
BbvI GCAGC 1 cut(s) 457
BccI CCATC 4 cut(s) 400, 644, 841, 1229
BceAI ACGGC 2 cut(s) 385, 1212
BciT130I CCWGG 2 cut(s) 186, 840
BclI TGATCA 1 cut(s) 610
BcnI CCSGG 1 cut(s) 1101
BfmI CTRYAG 1 cut(s) 694
BfoI RGCGCY 1 cut(s) 415
BisI GCNGC 5 cut(s) 68, 77, 350, 446, 506
BlsI GCNGC 5 cut(s) 69, 78, 351, 447, 507
Bme1390I CCNGG 3 cut(s) 186, 840, 1101
Bme18I GGWCC 2 cut(s) 127, 284
BmgBI CACGTC 1 cut(s) 83
BmgT120I GGNCC 5 cut(s) 127, 188, 284, 483, 917
BmrFI CCNGG 3 cut(s) 186, 840, 1101
BmsI GCATC 2 cut(s) 538, 628
BpiI GAAGAC 1 cut(s) 658
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 3 cut(s) 85, 608, 939
BpuMI CCSGG 1 cut(s) 1101
BsaAI YACGTR 1 cut(s) 1046
BsaJI CCNNGG 2 cut(s) 391, 1100
BsaXI ACNNNNNCTCC 6 cut(s) 303, 333, 365, 395, 834, 864
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 408, 492, 839
Bse1I ACTGG 1 cut(s) 731
Bse21I CCTNAGG 2 cut(s) 781, 1134
BseBI CCWGG 2 cut(s) 186, 840
BseDI CCNNGG 2 cut(s) 391, 1100
BseGI GGATG 3 cut(s) 109, 823, 932
BseLI CCNNNNNNNGG 4 cut(s) 185, 408, 492, 839
BseMII CTCAG 2 cut(s) 482, 795
BseNI ACTGG 1 cut(s) 731
BseRI GAGGAG 8 cut(s) 153, 156, 159, 162, 205, 324, 327, 489
BseXI GCAGC 1 cut(s) 457
BsgI GTGCAG 1 cut(s) 1195
BshFI GGCC 6 cut(s) 189, 372, 484, 496, 762, 919
BsiHKAI GWGCWC 2 cut(s) 20, 301
BsiSI CCGG 1 cut(s) 1100
BslFI GGGAC 1 cut(s) 725
BslI CCNNNNNNNGG 4 cut(s) 185, 408, 492, 839
BsmFI GGGAC 1 cut(s) 725
BsmI GAATGC 1 cut(s) 1115
BsnI GGCC 6 cut(s) 189, 372, 484, 496, 762, 919
Bsp1286I GDGCHC 2 cut(s) 20, 301
Bsp143I GATC 3 cut(s) 610, 820, 1254
Bsp19I CCATGG 1 cut(s) 391
BspANI GGCC 6 cut(s) 189, 372, 484, 496, 762, 919
BspCNI CTCAG 2 cut(s) 481, 794
BspQI GCTCTTC 1 cut(s) 678
BsrI ACTGG 1 cut(s) 731
BssECI CCNNGG 2 cut(s) 391, 1100
BssMI GATC 3 cut(s) 610, 820, 1254
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 2 cut(s) 186, 840
Bst4CI ACNGT 5 cut(s) 664, 695, 1017, 1075, 1184
Bst6I CTCTTC 1 cut(s) 678
BstBAI YACGTR 1 cut(s) 1046
BstC8I GCNNGC 1 cut(s) 486
BstDEI CTNAG 4 cut(s) 468, 678, 781, 1134
BstDSI CCRYGG 1 cut(s) 391
BstEII GGTNACC 1 cut(s) 980
BstF5I GGATG 3 cut(s) 109, 823, 932
BstH2I RGCGCY 1 cut(s) 415
BstHHI GCGC 1 cut(s) 414
BstKTI GATC 3 cut(s) 613, 823, 1257
BstMBI GATC 3 cut(s) 610, 820, 1254
BstMWI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 260, 393, 502, 511, 1119, 1224
BstNI CCWGG 2 cut(s) 186, 840
BstNSI RCATGY 1 cut(s) 882
BstPI GGTNACC 1 cut(s) 980
BstSCI CCNGG 3 cut(s) 184, 838, 1099
BstSFI CTRYAG 1 cut(s) 694
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 1 cut(s) 658
Bsu36I CCTNAGG 2 cut(s) 781, 1134
BsuRI GGCC 6 cut(s) 189, 372, 484, 496, 762, 919
BtgI CCRYGG 1 cut(s) 391
BtrI CACGTC 1 cut(s) 83
BtsCI GGATG 3 cut(s) 109, 823, 932
BtsI GCAGTG 2 cut(s) 1140, 1183
BtsIMutI CAGTG 5 cut(s) 738, 1080, 1092, 1140, 1183
Cac8I GCNNGC 1 cut(s) 486
CaiI CAGNNNCTG 2 cut(s) 1015, 1182
CfoI GCGC 1 cut(s) 414
Cfr13I GGNCC 5 cut(s) 127, 188, 284, 483, 917
Csp6I GTAC 1 cut(s) 304
CviAII CATG 7 cut(s) 12, 131, 392, 403, 643, 879, 1123
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 4 cut(s) 468, 678, 781, 1134
DpnI GATC 3 cut(s) 612, 822, 1256
DpnII GATC 3 cut(s) 610, 820, 1254
EaeI YGGCCR 2 cut(s) 370, 760
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
EciI GGCGGA 5 cut(s) 54, 250, 336, 492, 501
Ecl136II GAGCTC 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 301
Eco47I GGWCC 2 cut(s) 127, 284
Eco53kI GAGCTC 1 cut(s) 299
Eco57I CTGAAG 2 cut(s) 400, 738
Eco81I CCTNAGG 2 cut(s) 781, 1134
Eco91I GGTNACC 1 cut(s) 980
EcoICRI GAGCTC 1 cut(s) 299
EcoO65I GGTNACC 1 cut(s) 980
EcoRII CCWGG 2 cut(s) 184, 838
EcoT14I CCWWGG 1 cut(s) 391
EcoT22I ATGCAT 1 cut(s) 1124
EcoT38I GRGCYC 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 7 cut(s) 15, 134, 395, 406, 646, 882, 1126
FaqI GGGAC 1 cut(s) 725
FatI CATG 7 cut(s) 11, 130, 391, 402, 642, 878, 1122
FauI CCCGC 2 cut(s) 493, 1191
FauNDI CATATG 3 cut(s) 250, 942, 1164
FbaI TGATCA 1 cut(s) 610
FblI GTMKAC 1 cut(s) 1159
Fnu4HI GCNGC 5 cut(s) 68, 77, 350, 446, 506
FokI GGATG 3 cut(s) 96, 830, 919
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 5 cut(s) 68, 77, 350, 446, 506
GlaI GCGC 1 cut(s) 413
GluI GCNGC 5 cut(s) 68, 77, 350, 446, 506
HaeII RGCGCY 1 cut(s) 415
HaeIII GGCC 6 cut(s) 189, 372, 484, 496, 762, 919
HapII CCGG 1 cut(s) 1100
HhaI GCGC 1 cut(s) 414
Hin1II CATG 7 cut(s) 15, 134, 395, 406, 646, 882, 1126
Hin6I GCGC 1 cut(s) 412
HinP1I GCGC 1 cut(s) 412
HincII GTYRAC 4 cut(s) 86, 562, 1160, 1232
HindII GTYRAC 4 cut(s) 86, 562, 1160, 1232
HindIII AAGCTT 1 cut(s) 1081
HinfI GANTC 2 cut(s) 738, 893
HpaII CCGG 1 cut(s) 1100
HphI GGTGA 2 cut(s) 828, 992
Hpy166II GTNNAC 4 cut(s) 86, 562, 1160, 1232
Hpy188I TCNGA 4 cut(s) 127, 471, 534, 750
Hpy188III TCNNGA 3 cut(s) 716, 783, 1258
Hpy8I GTNNAC 4 cut(s) 86, 562, 1160, 1232
HpyAV CCTTC 5 cut(s) 24, 351, 538, 745, 1099
HpyCH4III ACNGT 5 cut(s) 664, 695, 1017, 1075, 1184
HpyCH4IV ACGT 5 cut(s) 82, 522, 564, 578, 1045
HpyCH4V TGCA 6 cut(s) 387, 568, 946, 1122, 1176, 1187
HpyF10VI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 260, 393, 502, 511, 1119, 1224
HpyF3I CTNAG 4 cut(s) 468, 678, 781, 1134
HpySE526I ACGT 5 cut(s) 82, 522, 564, 578, 1045
Hsp92II CATG 7 cut(s) 15, 134, 395, 406, 646, 882, 1126
HspAI GCGC 1 cut(s) 412
Ksp22I TGATCA 1 cut(s) 610
Kzo9I GATC 3 cut(s) 610, 820, 1254
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 3 cut(s) 15, 304, 654
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 2 cut(s) 538, 628
MaeII ACGT 5 cut(s) 82, 522, 564, 578, 1045
MaeIII GTNAC 3 cut(s) 419, 980, 1178
MalI GATC 3 cut(s) 612, 822, 1256
MboI GATC 3 cut(s) 610, 820, 1254
MboII GAAGA 7 cut(s) 39, 198, 201, 222, 658, 695, 857
MhlI GDGCHC 2 cut(s) 20, 301
MlsI TGGCCA 1 cut(s) 762
MluNI TGGCCA 1 cut(s) 762
MlyI GAGTC 1 cut(s) 747
MmeI TCCRAC 1 cut(s) 795
Mox20I TGGCCA 1 cut(s) 762
Mph1103I ATGCAT 1 cut(s) 1124
MscI TGGCCA 1 cut(s) 762
MseI TTAA 1 cut(s) 549
MslI CAYNNNNRTG 1 cut(s) 1145
Msp20I TGGCCA 1 cut(s) 762
MspI CCGG 1 cut(s) 1100
MspR9I CCNGG 3 cut(s) 186, 840, 1101
Mva1269I GAATGC 1 cut(s) 1115
MvaI CCWGG 2 cut(s) 186, 840
MwoI GCNNNNNNNGC 9 cut(s) 67, 73, 76, 260, 393, 502, 511, 1119, 1224
NciI CCSGG 1 cut(s) 1101
NcoI CCATGG 1 cut(s) 391
NdeI CATATG 3 cut(s) 250, 942, 1164
NdeII GATC 3 cut(s) 610, 820, 1254
NlaIII CATG 7 cut(s) 15, 134, 395, 406, 646, 882, 1126
NmuCI GTSAC 2 cut(s) 980, 1178
NsiI ATGCAT 1 cut(s) 1124
NspI RCATGY 1 cut(s) 882
OliI CACNNNNGTG 1 cut(s) 1145
PciI ACATGT 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 678
PcsI WCGNNNNNNNCGW 1 cut(s) 561
PctI GAATGC 1 cut(s) 1115
PfeI GAWTC 1 cut(s) 893
PkrI GCNGC 5 cut(s) 69, 78, 351, 447, 507
PleI GAGTC 1 cut(s) 746
PpsI GAGTC 1 cut(s) 746
Ppu21I YACGTR 1 cut(s) 1046
PscI ACATGT 1 cut(s) 878
PsiI TTATAA 1 cut(s) 453
Psp124BI GAGCTC 1 cut(s) 301
Psp6I CCWGG 2 cut(s) 184, 838
PspEI GGTNACC 1 cut(s) 980
PspGI CCWGG 2 cut(s) 184, 838
PspPI GGNCC 5 cut(s) 127, 188, 284, 483, 917
PsrI GAACNNNNNNTAC 2 cut(s) 26, 58
PstNI CAGNNNCTG 2 cut(s) 1015, 1182
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 1145
SacI GAGCTC 1 cut(s) 301
SalI GTCGAC 1 cut(s) 1158
SapI GCTCTTC 1 cut(s) 678
SaqAI TTAA 1 cut(s) 549
SatI GCNGC 5 cut(s) 68, 77, 350, 446, 506
Sau3AI GATC 3 cut(s) 610, 820, 1254
Sau96I GGNCC 5 cut(s) 127, 188, 284, 483, 917
SchI GAGTC 1 cut(s) 747
ScrFI CCNGG 3 cut(s) 186, 840, 1101
SduI GDGCHC 2 cut(s) 20, 301
SfaNI GCATC 2 cut(s) 538, 628
SfcI CTRYAG 1 cut(s) 694
SinI GGWCC 2 cut(s) 127, 284
SmiMI CAYNNNNRTG 1 cut(s) 1145
SmlI CTYRAG 3 cut(s) 100, 587, 954
SmoI CTYRAG 3 cut(s) 100, 587, 954
SstI GAGCTC 1 cut(s) 301
StyD4I CCNGG 3 cut(s) 184, 838, 1099
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 5 cut(s) 664, 695, 1017, 1075, 1184
TaiI ACGT 5 cut(s) 85, 525, 567, 581, 1048
TaqI TCGA 3 cut(s) 636, 1070, 1159
TauI GCSGC 4 cut(s) 70, 79, 352, 508
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 1 cut(s) 549
Tru9I TTAA 1 cut(s) 549
TscAI CASTG 5 cut(s) 738, 1080, 1099, 1147, 1183
TseFI GTSAC 2 cut(s) 980, 1178
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 2 cut(s) 980, 1178
TspDTI ATGAA 2 cut(s) 213, 1041
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 5 cut(s) 738, 1080, 1099, 1147, 1183
VpaK11BI GGWCC 2 cut(s) 127, 284
XapI RAATTY 1 cut(s) 767
XceI RCATGY 1 cut(s) 882
XmiI GTMKAC 1 cut(s) 1159
Zsp2I ATGCAT 1 cut(s) 1124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.