AT3G08900

Reversibly glycosylated polypeptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008993
Physical Location & Seq
Reverse (-)
2708086 .. 2709949
1864 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G08900.1

Sequence Viewer

Length: 1089 bp
ATGGCGCAATTGTATTCTTCCGTGAAACCCACGCCAATGTTGAAAGATGAGCTAGACATTGTGATACCTACGATTCGAAACCTCGACTTCCTTGAGATGTGGCGACCTTTCTTTGAGCAGTATCATTTGATCATTGTTCAAGATGGTGATCCTTCGAAAGTCATCAACATTCCAGTAGGGTTTGACTACGAGCTCTACAACAGGAACGACATCAATCGAATCTTGGGTCCCAAGGCCTCTTGCATTTCCTTCAAGGACTCTGCTTGTCGTTGCTTCGGTTACATGGTCTCCAAAAAGAAGTACATCTACACCATTGATGATGATTGCTTTGTTGCAAAGGATCCAACAGGGAAAGAGATCAATGCGCTTGAGCAGCATATCAAGAACCTCTTATCTCCATCAACTCCGCATTTCTTTAACACACTTTATGATCCATACAGAGATGGGGCAGACTTTGTTCGTGGATACCCTTTCAGTATGCGCGAAGGTGCCATAACCGCGGTCTCTCATGGCCTCTGGCTCAACATTCCTGACTATGATGCTCCCACTCAGCTTGTGAAACCTCTTGAGAAAAATTCCAGGTATGTTGATGCAGTCATGACCATTCCCAAAGGAACTCTTTTTCCTATGTGTGGTATGAACCTTGCTTTTGACCGTGAGCTGATTGGTCCGGCTATGTACTTTGGGCTTATGGGTGATGGACAGCCAATTGGACGCTATGACGACATGTGGGCTGGTTGGTGTGTCAAGGTGATATGTGACCACATGGGATGGGGAGTGAAGACGGGTTTGCCTTACATATGGCACAGCAAAGCCAGCAACCCATTCGTGAACCTGAAAAAGGAATACAACGGAATATTTTGGCAGGAAGAGGCGATTCCGTTCTTTCAATCCGTGACTCTTCCTAAAGAGTGCACTTCGGTGCAGCAATGCTACCTCGAGCTGGCCAAGCTCGTGAGAGAGAAACTTGGGAAGGTTGATCCTTACTTCATCACACTTGCAACTGGAATGGTCACCTGGATCGAAGCTTGGGAGGAGCTTAACTCCGCGGAGGGAACTGAGGCTGAGGCACCAAAAGGCAAAAATTAG

Protein Analysis

362

Amino Acids

41.28

Weight (kDa)

5.39

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 14 - 348 6.6e-177 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 488, 1069
AccII CGCG 3 cut(s) 483, 500, 1049
AciI CCGC 5 cut(s) 407, 498, 500, 1047, 1049
AclWI GGATC 6 cut(s) 143, 335, 348, 425, 974, 1028
AcoI YGGCCR 1 cut(s) 945
AcsI RAATTY 1 cut(s) 574
AfaI GTAC 2 cut(s) 302, 680
AfiI CCNNNNNNNGG 3 cut(s) 632, 841, 943
AflIII ACRYGT 1 cut(s) 726
AgsI TTSAA 4 cut(s) 43, 140, 253, 890
AjnI CCWGG 2 cut(s) 578, 1016
AleI CACNNNNGTG 1 cut(s) 920
AloI GAACNNNNNNTCC 2 cut(s) 607, 639
AluBI AGCT 8 cut(s) 52, 193, 553, 661, 943, 952, 1028, 1039
AluI AGCT 8 cut(s) 52, 193, 553, 661, 943, 952, 1028, 1039
Alw21I GWGCWC 2 cut(s) 195, 917
Alw26I GTCTC 2 cut(s) 292, 508
Alw44I GTGCAC 1 cut(s) 913
AlwI GGATC 6 cut(s) 143, 335, 348, 425, 974, 1028
Ama87I CYCGRG 1 cut(s) 938
AoxI GGCC 3 cut(s) 234, 511, 945
ApaLI GTGCAC 1 cut(s) 913
ApeKI GCWGC 2 cut(s) 373, 925
ApoI RAATTY 1 cut(s) 574
ArsI GACNNNNNNTTYG 2 cut(s) 211, 243
AspLEI GCGC 3 cut(s) 7, 367, 483
AspS9I GGNCC 2 cut(s) 227, 668
AsuHPI GGTGA 4 cut(s) 158, 707, 763, 1006
AsuII TTCGAA 2 cut(s) 76, 155
AvaI CYCGRG 1 cut(s) 938
AvaII GGWCC 2 cut(s) 227, 668
BaeGI GKGCMC 1 cut(s) 917
BalI TGGCCA 1 cut(s) 947
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 2 cut(s) 488, 1069
BanII GRGCYC 1 cut(s) 195
BarI GAAGNNNNNNTAC 2 cut(s) 290, 322
BauI CACGAG 1 cut(s) 953
BbsI GAAGAC 1 cut(s) 788
Bbv12I GWGCWC 2 cut(s) 195, 917
BbvCI CCTCAGC 1 cut(s) 1065
BbvI GCAGC 2 cut(s) 385, 937
BccI CCATC 5 cut(s) 137, 406, 437, 692, 765
BcgI CGANNNNNNTGC 2 cut(s) 808, 842
BciT130I CCWGG 2 cut(s) 580, 1018
BciVI GTATCC 1 cut(s) 458
BclI TGATCA 1 cut(s) 129
BcoDI GTCTC 2 cut(s) 292, 508
BfaI CTAG 1 cut(s) 53
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 2 cut(s) 374, 926
BlsI GCNGC 2 cut(s) 375, 927
Bme1390I CCNGG 2 cut(s) 580, 1018
Bme18I GGWCC 2 cut(s) 227, 668
BmeT110I CYCGRG 1 cut(s) 938
BmgT120I GGNCC 2 cut(s) 227, 668
BmiI GGNNCC 5 cut(s) 228, 229, 342, 490, 1071
BmrFI CCNGG 2 cut(s) 580, 1018
BmsI GCATC 2 cut(s) 529, 580
BpiI GAAGAC 1 cut(s) 788
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
Bpu10I CCTNAGC 1 cut(s) 1065
Bpu14I TTCGAA 2 cut(s) 76, 155
BpuEI CTTGAG 3 cut(s) 113, 389, 587
BsaBI GATNNNNATC 1 cut(s) 147
BsaI GGTCTC 2 cut(s) 292, 508
BsaJI CCNNGG 3 cut(s) 231, 498, 1047
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 632, 841, 943
Bse1I ACTGG 2 cut(s) 173, 1009
Bse3DI GCAATG 1 cut(s) 935
Bse8I GATNNNNATC 1 cut(s) 147
BseBI CCWGG 2 cut(s) 580, 1018
BseDI CCNNGG 3 cut(s) 231, 498, 1047
BseGI GGATG 1 cut(s) 776
BseJI GATNNNNATC 1 cut(s) 147
BseLI CCNNNNNNNGG 3 cut(s) 632, 841, 943
BseMI GCAATG 1 cut(s) 935
BseMII CTCAG 3 cut(s) 563, 1050, 1056
BseNI ACTGG 2 cut(s) 173, 1009
BseRI GAGGAG 1 cut(s) 1049
BseSI GKGCMC 1 cut(s) 917
BseXI GCAGC 2 cut(s) 385, 937
BsgI GTGCAG 1 cut(s) 944
Bsh1236I CGCG 3 cut(s) 483, 500, 1049
BshFI GGCC 3 cut(s) 236, 513, 947
BshNI GGYRCC 2 cut(s) 488, 1069
BsiHKAI GWGCWC 2 cut(s) 195, 917
BsiHKCI CYCGRG 1 cut(s) 938
BsiSI CCGG 1 cut(s) 671
BslFI GGGAC 1 cut(s) 213
BslI CCNNNNNNNGG 3 cut(s) 632, 841, 943
BsmAI GTCTC 2 cut(s) 292, 508
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 3 cut(s) 236, 513, 947
Bso31I GGTCTC 2 cut(s) 292, 508
BsoBI CYCGRG 1 cut(s) 938
Bsp119I TTCGAA 2 cut(s) 76, 155
Bsp1286I GDGCHC 2 cut(s) 195, 917
Bsp143I GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
BspACI CCGC 5 cut(s) 407, 498, 500, 1047, 1049
BspANI GGCC 3 cut(s) 236, 513, 947
BspCNI CTCAG 3 cut(s) 562, 1051, 1057
BspFNI CGCG 3 cut(s) 483, 500, 1049
BspHI TCATGA 1 cut(s) 597
BspLI GGNNCC 5 cut(s) 228, 229, 342, 490, 1071
BspPI GGATC 6 cut(s) 143, 335, 348, 425, 974, 1028
BspT104I TTCGAA 2 cut(s) 76, 155
BspT107I GGYRCC 2 cut(s) 488, 1069
BspTNI GGTCTC 2 cut(s) 292, 508
BsrDI GCAATG 1 cut(s) 935
BsrI ACTGG 2 cut(s) 173, 1009
BssECI CCNNGG 3 cut(s) 231, 498, 1047
BssMI GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
BssSI CACGAG 1 cut(s) 953
BssT1I CCWWGG 1 cut(s) 231
Bst2BI CACGAG 1 cut(s) 953
Bst2UI CCWGG 2 cut(s) 580, 1018
Bst4CI ACNGT 1 cut(s) 656
Bst6I CTCTTC 2 cut(s) 864, 906
BstBI TTCGAA 2 cut(s) 76, 155
BstC8I GCNNGC 2 cut(s) 817, 945
BstDEI CTNAG 3 cut(s) 549, 1059, 1065
BstDSI CCRYGG 2 cut(s) 498, 1047
BstEII GGTNACC 1 cut(s) 1012
BstENI CCTNNNNNAGG 1 cut(s) 839
BstF5I GGATG 1 cut(s) 776
BstFNI CGCG 3 cut(s) 483, 500, 1049
BstHHI GCGC 3 cut(s) 7, 367, 483
BstKTI GATC 7 cut(s) 132, 151, 343, 360, 433, 982, 1023
BstMAI GTCTC 2 cut(s) 292, 508
BstMBI GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
BstMWI GCNNNNNNNGC 4 cut(s) 373, 497, 816, 949
BstNI CCWGG 2 cut(s) 580, 1018
BstNSI RCATGY 1 cut(s) 730
BstPI GGTNACC 1 cut(s) 1012
BstSCI CCNGG 2 cut(s) 578, 1016
BstSLI GKGCMC 1 cut(s) 917
BstUI CGCG 3 cut(s) 483, 500, 1049
BstV1I GCAGC 2 cut(s) 385, 937
BstV2I GAAGAC 1 cut(s) 788
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 3 cut(s) 236, 513, 947
BtgI CCRYGG 2 cut(s) 498, 1047
BtsCI GGATG 1 cut(s) 776
Cac8I GCNNGC 2 cut(s) 817, 945
CciI TCATGA 1 cut(s) 597
CfoI GCGC 3 cut(s) 7, 367, 483
Cfr13I GGNCC 2 cut(s) 227, 668
Cfr42I CCGCGG 2 cut(s) 501, 1050
CseI GACGC 1 cut(s) 723
Csp6I GTAC 2 cut(s) 301, 679
CspCI CAANNNNNGTGG 2 cut(s) 535, 570
CviAII CATG 5 cut(s) 283, 509, 598, 727, 766
CviQI GTAC 2 cut(s) 301, 679
DdeI CTNAG 3 cut(s) 549, 1059, 1065
DpnI GATC 7 cut(s) 131, 150, 342, 359, 432, 981, 1022
DpnII GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
EaeI YGGCCR 1 cut(s) 945
Eam1104I CTCTTC 2 cut(s) 864, 906
EarI CTCTTC 2 cut(s) 864, 906
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 231
Eco147I AGGCCT 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 195
Eco31I GGTCTC 2 cut(s) 292, 508
Eco47I GGWCC 2 cut(s) 227, 668
Eco53kI GAGCTC 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 938
Eco91I GGTNACC 1 cut(s) 1012
EcoICRI GAGCTC 1 cut(s) 193
EcoNI CCTNNNNNAGG 1 cut(s) 839
EcoO109I RGGNCCY 1 cut(s) 227
EcoO65I GGTNACC 1 cut(s) 1012
EcoRII CCWGG 2 cut(s) 578, 1016
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 5 cut(s) 286, 512, 601, 730, 769
FalI AAGNNNNNCTT 4 cut(s) 374, 406, 603, 635
FaqI GGGAC 1 cut(s) 213
FatI CATG 5 cut(s) 282, 508, 597, 726, 765
FauNDI CATATG 1 cut(s) 800
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 2 cut(s) 374, 926
FokI GGATG 1 cut(s) 783
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 2 cut(s) 374, 926
FspBI CTAG 1 cut(s) 53
GlaI GCGC 3 cut(s) 6, 366, 482
GluI GCNGC 2 cut(s) 374, 926
HaeIII GGCC 3 cut(s) 236, 513, 947
HapII CCGG 1 cut(s) 671
HgaI GACGC 1 cut(s) 723
HhaI GCGC 3 cut(s) 7, 367, 483
Hin1II CATG 5 cut(s) 286, 512, 601, 730, 769
Hin6I GCGC 3 cut(s) 5, 365, 481
HinP1I GCGC 3 cut(s) 5, 365, 481
HindIII AAGCTT 1 cut(s) 1026
HinfI GANTC 5 cut(s) 73, 219, 257, 877, 898
HpaII CCGG 1 cut(s) 671
HphI GGTGA 4 cut(s) 158, 707, 763, 1006
Hpy166II GTNNAC 2 cut(s) 832, 915
Hpy188III TCNNGA 7 cut(s) 140, 382, 530, 566, 598, 829, 955
Hpy8I GTNNAC 2 cut(s) 832, 915
HpyAV CCTTC 4 cut(s) 162, 259, 479, 967
HpyCH4III ACNGT 1 cut(s) 656
HpyCH4V TGCA 6 cut(s) 243, 335, 593, 915, 925, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 373, 497, 816, 949
HpyF3I CTNAG 3 cut(s) 549, 1059, 1065
Hsp92II CATG 5 cut(s) 286, 512, 601, 730, 769
HspAI GCGC 3 cut(s) 5, 365, 481
KflI GGGWCCC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 129
KspI CCGCGG 2 cut(s) 501, 1050
Kzo9I GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
LmnI GCTCC 2 cut(s) 547, 1036
Lsp1109I GCAGC 2 cut(s) 385, 937
LweI GCATC 2 cut(s) 529, 580
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 4 cut(s) 278, 758, 895, 1012
MalI GATC 7 cut(s) 131, 150, 342, 359, 432, 981, 1022
MboI GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
MboII GAAGA 4 cut(s) 9, 793, 881, 893
MfeI CAATTG 2 cut(s) 8, 708
MflI RGATCY 1 cut(s) 340
MhlI GDGCHC 2 cut(s) 195, 917
MlsI TGGCCA 1 cut(s) 947
MluCI AATT 4 cut(s) 8, 574, 708, 1084
MluNI TGGCCA 1 cut(s) 947
MlyI GAGTC 2 cut(s) 251, 892
MmeI TCCRAC 1 cut(s) 368
Mox20I TGGCCA 1 cut(s) 947
MscI TGGCCA 1 cut(s) 947
MseI TTAA 2 cut(s) 417, 1041
MslI CAYNNNNRTG 2 cut(s) 35, 920
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 2 cut(s) 500, 1049
MspI CCGG 1 cut(s) 671
MspR9I CCNGG 2 cut(s) 580, 1018
MunI CAATTG 2 cut(s) 8, 708
MvaI CCWGG 2 cut(s) 580, 1018
MvnI CGCG 3 cut(s) 483, 500, 1049
MwoI GCNNNNNNNGC 4 cut(s) 373, 497, 816, 949
NdeI CATATG 1 cut(s) 800
NdeII GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
NlaIII CATG 5 cut(s) 286, 512, 601, 730, 769
NlaIV GGNNCC 5 cut(s) 228, 229, 342, 490, 1071
NmuCI GTSAC 3 cut(s) 758, 895, 1012
NspI RCATGY 1 cut(s) 730
NspV TTCGAA 2 cut(s) 76, 155
OliI CACNNNNGTG 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 938
PagI TCATGA 1 cut(s) 597
PceI AGGCCT 1 cut(s) 236
PciI ACATGT 1 cut(s) 726
PfeI GAWTC 3 cut(s) 73, 219, 877
PkrI GCNGC 2 cut(s) 375, 927
PleI GAGTC 2 cut(s) 251, 892
PpsI GAGTC 2 cut(s) 251, 892
PpuMI RGGWCCY 1 cut(s) 227
PscI ACATGT 1 cut(s) 726
Psp124BI GAGCTC 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 227
Psp6I CCWGG 2 cut(s) 578, 1016
PspEI GGTNACC 1 cut(s) 1012
PspGI CCWGG 2 cut(s) 578, 1016
PspN4I GGNNCC 5 cut(s) 228, 229, 342, 490, 1071
PspPI GGNCC 2 cut(s) 227, 668
PspPPI RGGWCCY 1 cut(s) 227
PspXI VCTCGAGB 1 cut(s) 938
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 2 cut(s) 302, 680
RsaNI GTAC 2 cut(s) 301, 679
RseI CAYNNNNRTG 2 cut(s) 35, 920
SacI GAGCTC 1 cut(s) 195
SacII CCGCGG 2 cut(s) 501, 1050
SaqAI TTAA 2 cut(s) 417, 1041
SatI GCNGC 2 cut(s) 374, 926
Sau3AI GATC 7 cut(s) 129, 148, 340, 357, 430, 979, 1020
Sau96I GGNCC 2 cut(s) 227, 668
SchI GAGTC 2 cut(s) 251, 892
ScrFI CCNGG 2 cut(s) 580, 1018
SduI GDGCHC 2 cut(s) 195, 917
SfaNI GCATC 2 cut(s) 529, 580
Sfr274I CTCGAG 1 cut(s) 938
Sfr303I CCGCGG 2 cut(s) 501, 1050
SfuI TTCGAA 2 cut(s) 76, 155
SgrBI CCGCGG 2 cut(s) 501, 1050
SinI GGWCC 2 cut(s) 227, 668
SlaI CTCGAG 1 cut(s) 938
SmiMI CAYNNNNRTG 2 cut(s) 35, 920
SmlI CTYRAG 4 cut(s) 92, 368, 566, 938
SmoI CTYRAG 4 cut(s) 92, 368, 566, 938
Sse9I AATT 4 cut(s) 8, 574, 708, 1084
SseBI AGGCCT 1 cut(s) 236
SsiI CCGC 5 cut(s) 407, 498, 500, 1047, 1049
SspI AATATT 1 cut(s) 858
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 236
StyD4I CCNGG 2 cut(s) 578, 1016
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 1 cut(s) 656
TaqI TCGA 6 cut(s) 76, 84, 155, 217, 939, 1023
TasI AATT 4 cut(s) 8, 574, 708, 1084
TatI WGTACW 2 cut(s) 300, 678
TfiI GAWTC 3 cut(s) 73, 219, 877
Tru1I TTAA 2 cut(s) 417, 1041
Tru9I TTAA 2 cut(s) 417, 1041
TseFI GTSAC 3 cut(s) 758, 895, 1012
TseI GCWGC 2 cut(s) 373, 925
Tsp45I GTSAC 3 cut(s) 758, 895, 1012
TspDTI ATGAA 2 cut(s) 653, 979
TspGWI ACGGA 4 cut(s) 10, 867, 870, 883
VneI GTGCAC 1 cut(s) 913
VpaK11BI GGWCC 2 cut(s) 227, 668
XagI CCTNNNNNAGG 1 cut(s) 839
XapI RAATTY 1 cut(s) 574
XceI RCATGY 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 938
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.