MD14G1215200.v1.1

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0008993
Physical Location & Seq
Forward (+)
29875720 .. 29878184
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1215200.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCGGAGTACAGCAAGGTCGCCCCGACCCCTCTGCTGAAAGACGAGCTCGACATCGTCATCCCCACCATCCGAAACCTTGATTTCCTCGAGATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGTGACCCGTCGAAGGTGATCAAGGTCCCGGAAGGCTTCGATTACGAGCTTTACAACCGCAACGACATCAACCGGATTCTGGGTCCCAAGGCGTCCTGCATCTCCTTCAAGGACTCTGCTTGCAGATGCTTCGGGTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTTGTTGCTAAGGATCCTTCTGGCAAAGATATCAATGCCCTTGAGCAGCATATCAAGAACCTTCTGTCTCCATCAACTCCATTTTTCTTCAACACCCTGTACGACCCATACCGTGAAGGGGCAGATTTCGTTCGTGGATACCCCTTCAGTCTGCGTGAAGGTATCCCAACTGCTGTTTCTCACGGCCTCTGGCTCAACATTCCGGATTATGATGCTCCCACCCAGCTTGTTAAGCCTCTAGAGAGAAACACCAGGTTTGTGGATGCAGTCCTGACCATTCCCAAGGGAACTCTCTTTCCCATGTGCGGCATGAATCTGGCATTCAACCGTGAGTTGATTGGACCTGCTATGTATTTCGGACTCATGGGTGATGGTCAGCCAATTGGGCGCTACGACGACATGTGGGCTGGCTGGTGCATGAAGGTTATCTGTGACCACTTGGGGTTGGGAGTGAAGACAGGATTGCCCTACATCTGGCATAGCAAGGCAAGCAACCCATTTGTGAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAAGAGGAGTTGATCCCCTTTTTCCAGTCCGCCACCCTCCCAAAGGATTGCACCACCGTCCAGGGCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCCAAACTTGGCAAGGTCGATGACTACTTCATCAAGCTTGCAGATGCCATGGTCACATGGGTCGAAGCATGGGATGAGTTGAACCCAGTCGAAGGAAAGGCTGTCGAGGCCAACGGAGTTGTTGCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

363

Amino Acids

41.14

Weight (kDa)

5.66

Isoelectric Point (pI)

28.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 347 3e-179 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 4 cut(s) 5, 199, 630, 891
AclWI GGATC 3 cut(s) 332, 345, 868
AcuI CTGAAG 1 cut(s) 454
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 5 cut(s) 11, 119, 278, 299, 425
AfiI CCNNNNNNNGG 8 cut(s) 154, 220, 442, 629, 798, 838, 904, 1049
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 5 cut(s) 250, 415, 649, 938, 1039
AjnI CCWGG 3 cut(s) 135, 575, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 4 cut(s) 49, 190, 550, 994
AluI AGCT 4 cut(s) 49, 190, 550, 994
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 289, 396
AlwI GGATC 3 cut(s) 332, 345, 868
Ama87I CYCGRG 1 cut(s) 89
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 4 cut(s) 101, 508, 957, 1065
ApeKI GCWGC 2 cut(s) 370, 927
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 4 cut(s) 102, 166, 224, 665
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 4 cut(s) 155, 169, 298, 704
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 3 cut(s) 166, 224, 665
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 51
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 382, 914
BccI CCATC 4 cut(s) 77, 317, 403, 689
BceAI ACGGC 1 cut(s) 523
BcgI CGANNNNNNTGC 4 cut(s) 16, 50, 253, 287
BciT130I CCWGG 3 cut(s) 137, 577, 923
BciVI GTATCC 2 cut(s) 455, 497
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 289, 396
BfaI CTAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 715
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 2 cut(s) 455, 497
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 3 cut(s) 371, 631, 928
BlsI GCNGC 3 cut(s) 372, 632, 929
Bme1390I CCNGG 4 cut(s) 137, 170, 577, 923
Bme18I GGWCC 3 cut(s) 166, 224, 665
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 4 cut(s) 102, 166, 224, 665
BmiI GGNNCC 4 cut(s) 168, 225, 226, 339
BmrFI CCNGG 4 cut(s) 137, 170, 577, 923
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 5 cut(s) 249, 257, 526, 577, 991
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 785
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 170
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 4 cut(s) 228, 606, 922, 1005
BsaWI WCCGGW 2 cut(s) 213, 526
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 8 cut(s) 154, 220, 442, 629, 798, 838, 904, 1049
Bse1I ACTGG 3 cut(s) 863, 886, 1043
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 3 cut(s) 137, 577, 923
BseDI CCNNGG 4 cut(s) 228, 606, 922, 1005
BseGI GGATG 4 cut(s) 60, 69, 592, 1036
BseLI CCNNNNNNNGG 8 cut(s) 154, 220, 442, 629, 798, 838, 904, 1049
BseNI ACTGG 3 cut(s) 863, 886, 1043
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 2 cut(s) 382, 914
BseYI CCCAGC 1 cut(s) 546
BshFI GGCC 4 cut(s) 103, 510, 959, 1067
BsiHKAI GWGCWC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 3 cut(s) 170, 214, 527
BslFI GGGAC 2 cut(s) 152, 210
BslI CCNNNNNNNGG 8 cut(s) 154, 220, 442, 629, 798, 838, 904, 1049
BsmAI GTCTC 2 cut(s) 289, 396
BsmFI GGGAC 2 cut(s) 152, 210
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 103, 510, 959, 1067
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 51
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 3 cut(s) 159, 337, 873
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 4 cut(s) 5, 199, 630, 891
BspANI GGCC 4 cut(s) 103, 510, 959, 1067
BspEI TCCGGA 1 cut(s) 526
BspLI GGNNCC 4 cut(s) 168, 225, 226, 339
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 332, 345, 868
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 3 cut(s) 863, 886, 1043
BssECI CCNNGG 4 cut(s) 228, 606, 922, 1005
BssMI GATC 3 cut(s) 159, 337, 873
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 3 cut(s) 228, 606, 1005
Bst2UI CCWGG 3 cut(s) 137, 577, 923
Bst4CI ACNGT 4 cut(s) 143, 437, 653, 919
BstACI GRCGYC 1 cut(s) 233
BstAFI CTTAAG 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 262, 733, 814, 996
BstDEI CTNAG 1 cut(s) 333
BstDSI CCRYGG 1 cut(s) 1005
BstEII GGTNACC 1 cut(s) 143
BstENI CCTNNNNNAGG 2 cut(s) 836, 902
BstF5I GGATG 4 cut(s) 60, 69, 592, 1036
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 3 cut(s) 162, 340, 876
BstMAI GTCTC 2 cut(s) 289, 396
BstMBI GATC 3 cut(s) 159, 337, 873
BstMWI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
BstNI CCWGG 3 cut(s) 137, 577, 923
BstNSI RCATGY 1 cut(s) 727
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 4 cut(s) 135, 168, 575, 921
BstV1I GCAGC 2 cut(s) 382, 914
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 337
BsuI GTATCC 2 cut(s) 455, 497
BsuRI GGCC 4 cut(s) 103, 510, 959, 1067
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 4 cut(s) 60, 69, 592, 1036
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 4 cut(s) 262, 733, 814, 996
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 4 cut(s) 102, 166, 224, 665
CseI GACGC 1 cut(s) 222
CsiI ACCWGGT 1 cut(s) 575
Csp6I GTAC 5 cut(s) 10, 118, 277, 298, 424
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 9 cut(s) 280, 625, 634, 688, 724, 742, 1006, 1014, 1026
CviQI GTAC 5 cut(s) 10, 118, 277, 298, 424
DdeI CTNAG 1 cut(s) 333
DpnI GATC 3 cut(s) 161, 339, 875
DpnII GATC 3 cut(s) 159, 337, 873
EciI GGCGGA 2 cut(s) 20, 880
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 228, 606, 1005
Eco24I GRGCYC 1 cut(s) 51
Eco31I GGTCTC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 166, 224, 665
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 454
Eco88I CYCGRG 1 cut(s) 89
Eco91I GGTNACC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 49
EcoNI CCTNNNNNAGG 2 cut(s) 836, 902
EcoO109I RGGNCCY 3 cut(s) 102, 166, 224
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 3 cut(s) 135, 575, 921
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 228, 606, 1005
EcoT38I GRGCYC 1 cut(s) 51
ErhI CCWWGG 3 cut(s) 228, 606, 1005
FaeI CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 2 cut(s) 152, 210
FatI CATG 9 cut(s) 279, 624, 633, 687, 723, 741, 1005, 1013, 1025
FbaI TGATCA 1 cut(s) 159
Fnu4HI GCNGC 3 cut(s) 371, 631, 928
FokI GGATG 4 cut(s) 47, 56, 599, 1043
FriOI GRGCYC 1 cut(s) 51
Fsp4HI GCNGC 3 cut(s) 371, 631, 928
FspBI CTAG 1 cut(s) 563
GlaI GCGC 1 cut(s) 713
GluI GCNGC 3 cut(s) 371, 631, 928
GsaI CCCAGC 1 cut(s) 550
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 4 cut(s) 103, 510, 959, 1067
HapII CCGG 3 cut(s) 170, 214, 527
HgaI GACGC 1 cut(s) 222
HhaI GCGC 1 cut(s) 714
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 5 cut(s) 217, 254, 637, 684, 852
HpaII CCGG 3 cut(s) 170, 214, 527
HphI GGTGA 4 cut(s) 155, 169, 298, 704
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 2 cut(s) 74, 683
Hpy188III TCNNGA 5 cut(s) 91, 379, 527, 563, 595
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 4 cut(s) 154, 317, 320, 722
HpyCH4III ACNGT 4 cut(s) 143, 437, 653, 919
HpyCH4V TGCA 6 cut(s) 240, 264, 590, 741, 912, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
HpyF3I CTNAG 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
HspAI GCGC 1 cut(s) 712
KflI GGGWCCC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 526
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 337, 873
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 2 cut(s) 382, 914
LweI GCATC 5 cut(s) 249, 257, 526, 577, 991
MabI ACCWGGT 1 cut(s) 575
MaeI CTAG 1 cut(s) 563
MaeIII GTNAC 3 cut(s) 143, 755, 1009
MalI GATC 3 cut(s) 161, 339, 875
MboI GATC 3 cut(s) 159, 337, 873
MboII GAAGA 5 cut(s) 100, 295, 304, 403, 790
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 1 cut(s) 705
MlyI GAGTC 2 cut(s) 248, 678
MnlI CCTC 9 cut(s) 42, 93, 98, 134, 521, 570, 859, 908, 1057
MroI TCCGGA 1 cut(s) 526
MseI TTAA 2 cut(s) 555, 834
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 3 cut(s) 170, 214, 527
MspR9I CCNGG 4 cut(s) 137, 170, 577, 923
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 3 cut(s) 137, 577, 923
MwoI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 3 cut(s) 159, 337, 873
NlaIII CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
NlaIV GGNNCC 4 cut(s) 168, 225, 226, 339
NmuCI GTSAC 3 cut(s) 143, 755, 1009
NspI RCATGY 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 89
PciI ACATGT 1 cut(s) 723
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 217, 637, 852
PflFI GACNNNGTC 1 cut(s) 56
PfoI TCCNGGA 2 cut(s) 135, 168
PkrI GCNGC 3 cut(s) 372, 632, 929
PleI GAGTC 2 cut(s) 248, 678
PpsI GAGTC 2 cut(s) 248, 678
PpuMI RGGWCCY 2 cut(s) 166, 224
PscI ACATGT 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 51
Psp5II RGGWCCY 2 cut(s) 166, 224
Psp6I CCWGG 3 cut(s) 135, 575, 921
PspEI GGTNACC 1 cut(s) 143
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 3 cut(s) 135, 575, 921
PspN4I GGNNCC 4 cut(s) 168, 225, 226, 339
PspPI GGNCC 4 cut(s) 102, 166, 224, 665
PspPPI RGGWCCY 2 cut(s) 166, 224
PsuI RGATCY 1 cut(s) 337
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 5 cut(s) 11, 119, 278, 299, 425
RsaNI GTAC 5 cut(s) 10, 118, 277, 298, 424
SacI GAGCTC 1 cut(s) 51
SaqAI TTAA 2 cut(s) 555, 834
SatI GCNGC 3 cut(s) 371, 631, 928
Sau3AI GATC 3 cut(s) 159, 337, 873
Sau96I GGNCC 4 cut(s) 102, 166, 224, 665
SchI GAGTC 2 cut(s) 248, 678
ScrFI CCNGG 4 cut(s) 137, 170, 577, 923
SduI GDGCHC 1 cut(s) 51
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 5 cut(s) 249, 257, 526, 577, 991
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 3 cut(s) 166, 224, 665
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 89, 365, 833
SmoI CTYRAG 3 cut(s) 89, 365, 833
Sse9I AATT 1 cut(s) 705
SsiI CCGC 4 cut(s) 5, 199, 630, 891
SspMI CTAG 1 cut(s) 563
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 4 cut(s) 135, 168, 575, 921
StyI CCWWGG 3 cut(s) 228, 606, 1005
TaaI ACNGT 4 cut(s) 143, 437, 653, 919
TaqI TCGA 9 cut(s) 51, 90, 152, 180, 312, 975, 1020, 1047, 1062
TasI AATT 1 cut(s) 705
TatI WGTACW 2 cut(s) 9, 297
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 3 cut(s) 217, 637, 852
Tru1I TTAA 2 cut(s) 555, 834
Tru9I TTAA 2 cut(s) 555, 834
TseFI GTSAC 3 cut(s) 143, 755, 1009
TseI GCWGC 2 cut(s) 370, 927
Tsp45I GTSAC 3 cut(s) 143, 755, 1009
TspDTI ATGAA 3 cut(s) 650, 758, 976
TspGWI ACGGA 1 cut(s) 1086
Tth111I GACNNNGTC 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 3 cut(s) 166, 224, 665
XagI CCTNNNNNAGG 2 cut(s) 836, 902
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.