RchiOBHm_Chr7g0183461

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008993
Physical Location & Seq
Forward (+)
4323095 .. 4325796
2702 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16368

Sequence Viewer

Length: 1095 bp
ATGGCGGAGTACAGCAAGGTCGCGCCGACGCCGCTGCTGAAGGATGAGCTGGACATAGTGATCCCGACGATCCGAAACCTGGACTTCTTGGAAATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCCAGCAAGGTCATCAAGGTCCCCGAGGGCTTCGACTACGAGCTTTACAACCGCAACGACATTAACCGGATTCTCGGCCCCAAAGCCGCCTGTATCTCCTTCAAGGACTCCGCCTGCCGCTGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTCGTTGCTAAAGATCCCACTGGAAAAGAGATCAATGCACTAGAGCAGCACATCAAGAACCTTCTGTGCCCGTCGACTCCATTTTTCTTTAACACCCTGTACGACCCATACCGTGAAGGTGCAGATTTTGTCCGCGGTTACCCTTTCAGTCTGCGTGAAGGTGTGCCAACCGCTGTTTCTCACGGCCTCTGGCTCAACATTCCTGATTATGATGCACCTACCCAGCTTGTTAAGCCTCTTGAGAGGAACACCAGGTTTGTGGATGCAGTCTTGACCATCCCCAAGGGAACTCTCTTTCCCATGTGTGGGATGAATCTGGCATTCAACCGTGAGTTGATCGGCCCTGCCATGTACTTTGGACTCATGGGCGATGGGCAGCCAATTGGGCGCTACGACGATATGTGGGCTGGATGGTGCATGAAGGTTATTTGTGACCACTTGGGGCTAGGAGTGAAGACGGGATTGCCCTACATCTGGCATAGCAAAGCCAGCAACCCATTTGTTAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAGGAAGAGTTGATCCCATTTTTCCAATCTGCTACGCTTCCCAAGGATTGCACCACTGTTCAGGCCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCGAAACTTGGTAAGGTTGATGACTACTTCAACAAGCTTGCAGATGCCATGGTTACATGGATCGAAGCATGGGATGAGTTGAACCCGGCTGGGGGGAAAGCTGTTGAGCTCAACGGACCTGCAAAATCTTCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

364

Amino Acids

41.33

Weight (kDa)

6.03

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 347 1e-179 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1084
AccI GTMKAC 1 cut(s) 398
AccII CGCG 2 cut(s) 23, 459
AciI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
AclWI GGATC 5 cut(s) 55, 64, 332, 868, 1025
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 5 cut(s) 11, 119, 299, 425, 677
AfiI CCNNNNNNNGG 8 cut(s) 79, 154, 629, 630, 798, 838, 1048, 1049
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 5 cut(s) 250, 649, 938, 988, 1039
AjnI CCWGG 3 cut(s) 78, 135, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 6 cut(s) 49, 190, 550, 994, 1058, 1066
AluI AGCT 6 cut(s) 49, 190, 550, 994, 1058, 1066
Alw21I GWGCWC 1 cut(s) 1068
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 5 cut(s) 55, 64, 332, 868, 1025
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 5 cut(s) 101, 223, 508, 664, 924
ApeKI GCWGC 4 cut(s) 34, 267, 370, 700
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 2 cut(s) 25, 714
AspS9I GGNCC 5 cut(s) 102, 166, 224, 665, 1073
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 1 cut(s) 298
AsuII TTCGAA 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 2 cut(s) 166, 1073
BaeGI GKGCMC 1 cut(s) 395
BanII GRGCYC 1 cut(s) 1068
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 1068
BbvI GCAGC 4 cut(s) 21, 254, 382, 712
BccI CCATC 4 cut(s) 317, 608, 689, 729
BceAI ACGGC 2 cut(s) 157, 523
BcgI CGANNNNNNTGC 3 cut(s) 16, 50, 1068
BciT130I CCWGG 3 cut(s) 80, 137, 577
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 2 cut(s) 365, 770
BfoI RGCGCY 1 cut(s) 715
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 1084
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 7 cut(s) 32, 35, 234, 265, 268, 371, 701
BlsI GCNGC 7 cut(s) 33, 36, 235, 266, 269, 372, 702
Bme1390I CCNGG 4 cut(s) 80, 137, 577, 1043
Bme18I GGWCC 2 cut(s) 166, 1073
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 5 cut(s) 102, 166, 224, 665, 1073
BmiI GGNNCC 2 cut(s) 168, 226
BmrFI CCNGG 4 cut(s) 80, 137, 577, 1043
BmsI GCATC 3 cut(s) 526, 577, 991
BpiI GAAGAC 1 cut(s) 785
Bpu14I TTCGAA 1 cut(s) 1088
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 1043
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 5 cut(s) 171, 457, 606, 903, 1005
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 8 cut(s) 79, 154, 629, 630, 798, 838, 1048, 1049
Bse1I ACTGG 2 cut(s) 349, 863
BseBI CCWGG 3 cut(s) 80, 137, 577
BseDI CCNNGG 5 cut(s) 171, 457, 606, 903, 1005
BseGI GGATG 6 cut(s) 49, 592, 600, 639, 740, 1036
BseLI CCNNNNNNNGG 8 cut(s) 79, 154, 629, 630, 798, 838, 1048, 1049
BseNI ACTGG 2 cut(s) 349, 863
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 4 cut(s) 21, 254, 382, 712
BseYI CCCAGC 3 cut(s) 149, 546, 1046
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 2 cut(s) 23, 459
BshFI GGCC 5 cut(s) 103, 225, 510, 666, 926
BsiHKAI GWGCWC 1 cut(s) 1068
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 214, 1043
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 8 cut(s) 79, 154, 629, 630, 798, 838, 1048, 1049
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 152
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 5 cut(s) 103, 225, 510, 666, 926
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 1088
Bsp1286I GDGCHC 2 cut(s) 395, 1068
Bsp143I GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
BspANI GGCC 5 cut(s) 103, 225, 510, 666, 926
BspFNI CGCG 2 cut(s) 23, 459
BspLI GGNNCC 2 cut(s) 168, 226
BspMI ACCTGC 1 cut(s) 1084
BspPI GGATC 5 cut(s) 55, 64, 332, 868, 1025
BspT104I TTCGAA 1 cut(s) 1088
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 2 cut(s) 349, 863
BssECI CCNNGG 5 cut(s) 171, 457, 606, 903, 1005
BssMI GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 606, 903, 1005
Bst2UI CCWGG 3 cut(s) 80, 137, 577
Bst4CI ACNGT 3 cut(s) 437, 653, 919
Bst6I CTCTTC 1 cut(s) 861
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 833
BstBI TTCGAA 1 cut(s) 1088
BstC8I GCNNGC 4 cut(s) 262, 814, 928, 996
BstDSI CCRYGG 2 cut(s) 457, 1005
BstEII GGTNACC 1 cut(s) 461
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 6 cut(s) 49, 592, 600, 639, 740, 1036
BstFNI CGCG 2 cut(s) 23, 459
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 2 cut(s) 25, 714
BstKTI GATC 7 cut(s) 63, 72, 340, 357, 663, 876, 1020
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
BstMWI GCNNNNNNNGC 6 cut(s) 31, 150, 273, 556, 709, 813
BstNI CCWGG 3 cut(s) 80, 137, 577
BstPI GGTNACC 1 cut(s) 461
BstSCI CCNGG 4 cut(s) 78, 135, 575, 1041
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 2 cut(s) 23, 459
BstV1I GCAGC 4 cut(s) 21, 254, 382, 712
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 5 cut(s) 103, 225, 510, 666, 926
BtgI CCRYGG 2 cut(s) 457, 1005
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 6 cut(s) 49, 592, 600, 639, 740, 1036
BtsIMutI CAGTG 2 cut(s) 342, 915
BveI ACCTGC 1 cut(s) 1084
Cac8I GCNNGC 4 cut(s) 262, 814, 928, 996
CfoI GCGC 2 cut(s) 25, 714
Cfr13I GGNCC 5 cut(s) 102, 166, 224, 665, 1073
Cfr42I CCGCGG 1 cut(s) 460
CseI GACGC 1 cut(s) 37
CsiI ACCWGGT 1 cut(s) 575
Csp6I GTAC 5 cut(s) 10, 118, 298, 424, 676
CviAII CATG 8 cut(s) 280, 625, 673, 688, 742, 1006, 1014, 1026
CviQI GTAC 5 cut(s) 10, 118, 298, 424, 676
DpnI GATC 7 cut(s) 62, 71, 339, 356, 662, 875, 1019
DpnII GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
EciI GGCGGA 2 cut(s) 20, 247
Ecl136II GAGCTC 1 cut(s) 1066
Eco130I CCWWGG 3 cut(s) 606, 903, 1005
Eco147I AGGCCT 1 cut(s) 926
Eco24I GRGCYC 1 cut(s) 1068
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 2 cut(s) 166, 1073
Eco53kI GAGCTC 1 cut(s) 1066
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 461
EcoICRI GAGCTC 1 cut(s) 1066
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoO109I RGGNCCY 2 cut(s) 102, 166
EcoO65I GGTNACC 1 cut(s) 461
EcoRII CCWGG 3 cut(s) 78, 135, 575
EcoT14I CCWWGG 3 cut(s) 606, 903, 1005
EcoT38I GRGCYC 1 cut(s) 1068
ErhI CCWWGG 3 cut(s) 606, 903, 1005
FaeI CATG 8 cut(s) 283, 628, 676, 691, 745, 1009, 1017, 1029
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 152
FatI CATG 8 cut(s) 279, 624, 672, 687, 741, 1005, 1013, 1025
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 7 cut(s) 32, 35, 234, 265, 268, 371, 701
FokI GGATG 6 cut(s) 56, 587, 599, 646, 747, 1043
FriOI GRGCYC 1 cut(s) 1068
Fsp4HI GCNGC 7 cut(s) 32, 35, 234, 265, 268, 371, 701
FspBI CTAG 2 cut(s) 365, 770
GlaI GCGC 2 cut(s) 24, 713
GluI GCNGC 7 cut(s) 32, 35, 234, 265, 268, 371, 701
GsaI CCCAGC 3 cut(s) 153, 550, 1050
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 5 cut(s) 103, 225, 510, 666, 926
HapII CCGG 2 cut(s) 214, 1043
HgaI GACGC 1 cut(s) 37
HhaI GCGC 2 cut(s) 25, 714
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 8 cut(s) 283, 628, 676, 691, 745, 1009, 1017, 1029
Hin6I GCGC 2 cut(s) 23, 712
HinP1I GCGC 2 cut(s) 23, 712
HincII GTYRAC 2 cut(s) 399, 829
HindII GTYRAC 2 cut(s) 399, 829
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 6 cut(s) 217, 254, 400, 637, 684, 852
HpaI GTTAAC 1 cut(s) 829
HpaII CCGG 2 cut(s) 214, 1043
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 2 cut(s) 399, 829
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 5 cut(s) 64, 379, 527, 563, 595
Hpy8I GTNNAC 2 cut(s) 399, 829
Hpy99I CGWCG 6 cut(s) 31, 70, 317, 320, 400, 722
HpyAV CCTTC 8 cut(s) 34, 115, 256, 395, 434, 476, 739, 832
HpyCH4III ACNGT 3 cut(s) 437, 653, 919
HpyCH4V TGCA 8 cut(s) 362, 446, 539, 590, 741, 912, 998, 1079
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 150, 273, 556, 709, 813
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 8 cut(s) 283, 628, 676, 691, 745, 1009, 1017, 1029
HspAI GCGC 2 cut(s) 23, 712
KspAI GTTAAC 1 cut(s) 829
KspI CCGCGG 1 cut(s) 460
Kzo9I GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
Lsp1109I GCAGC 4 cut(s) 21, 254, 382, 712
LweI GCATC 3 cut(s) 526, 577, 991
MabI ACCWGGT 1 cut(s) 575
MaeI CTAG 2 cut(s) 365, 770
MaeIII GTNAC 3 cut(s) 461, 755, 1009
MalI GATC 7 cut(s) 62, 71, 339, 356, 662, 875, 1019
MboI GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
MboII GAAGA 6 cut(s) 100, 295, 304, 790, 878, 1077
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 395, 1068
MluCI AATT 1 cut(s) 705
MlyI GAGTC 3 cut(s) 248, 394, 678
MnlI CCTC 6 cut(s) 93, 134, 166, 521, 561, 570
MseI TTAA 5 cut(s) 210, 414, 555, 828, 834
MspA1I CMGCKG 4 cut(s) 34, 267, 459, 497
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 2 cut(s) 214, 1043
MspR9I CCNGG 4 cut(s) 80, 137, 577, 1043
MunI CAATTG 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 3 cut(s) 80, 137, 577
MvnI CGCG 2 cut(s) 23, 459
MwoI GCNNNNNNNGC 6 cut(s) 31, 150, 273, 556, 709, 813
NciI CCSGG 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
NlaIII CATG 8 cut(s) 283, 628, 676, 691, 745, 1009, 1017, 1029
NlaIV GGNNCC 2 cut(s) 168, 226
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 755
NspV TTCGAA 1 cut(s) 1088
PceI AGGCCT 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 217, 637, 852
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 7 cut(s) 33, 36, 235, 266, 269, 372, 702
PleI GAGTC 3 cut(s) 248, 394, 678
PpsI GAGTC 3 cut(s) 248, 394, 678
PpuMI RGGWCCY 1 cut(s) 166
Psp124BI GAGCTC 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 78, 135, 575
PspEI GGTNACC 1 cut(s) 461
PspFI CCCAGC 3 cut(s) 149, 546, 1046
PspGI CCWGG 3 cut(s) 78, 135, 575
PspN4I GGNNCC 2 cut(s) 168, 226
PspPI GGNCC 5 cut(s) 102, 166, 224, 665, 1073
PspPPI RGGWCCY 1 cut(s) 166
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 5 cut(s) 11, 119, 299, 425, 677
RsaNI GTAC 5 cut(s) 10, 118, 298, 424, 676
SacI GAGCTC 1 cut(s) 1068
SacII CCGCGG 1 cut(s) 460
SalI GTCGAC 1 cut(s) 397
SaqAI TTAA 5 cut(s) 210, 414, 555, 828, 834
SatI GCNGC 7 cut(s) 32, 35, 234, 265, 268, 371, 701
Sau3AI GATC 7 cut(s) 60, 69, 337, 354, 660, 873, 1017
Sau96I GGNCC 5 cut(s) 102, 166, 224, 665, 1073
SchI GAGTC 3 cut(s) 248, 394, 678
ScrFI CCNGG 4 cut(s) 80, 137, 577, 1043
SduI GDGCHC 2 cut(s) 395, 1068
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 3 cut(s) 526, 577, 991
Sfr303I CCGCGG 1 cut(s) 460
SfuI TTCGAA 1 cut(s) 1088
SgrBI CCGCGG 1 cut(s) 460
SinI GGWCC 2 cut(s) 166, 1073
SmlI CTYRAG 2 cut(s) 563, 833
SmoI CTYRAG 2 cut(s) 563, 833
Sse9I AATT 1 cut(s) 705
SseBI AGGCCT 1 cut(s) 926
SsiI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
SspMI CTAG 2 cut(s) 365, 770
SstI GAGCTC 1 cut(s) 1068
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 4 cut(s) 78, 135, 575, 1041
StyI CCWWGG 3 cut(s) 606, 903, 1005
TaaI ACNGT 3 cut(s) 437, 653, 919
TaqI TCGA 5 cut(s) 180, 312, 398, 1020, 1088
TasI AATT 1 cut(s) 705
TatI WGTACW 3 cut(s) 9, 297, 675
TauI GCSGC 3 cut(s) 34, 236, 267
TfiI GAWTC 3 cut(s) 217, 637, 852
Tru1I TTAA 5 cut(s) 210, 414, 555, 828, 834
Tru9I TTAA 5 cut(s) 210, 414, 555, 828, 834
TscAI CASTG 2 cut(s) 349, 922
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 4 cut(s) 34, 267, 370, 700
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 2 cut(s) 650, 758
TspGWI ACGGA 1 cut(s) 1086
TspRI CASTG 2 cut(s) 349, 922
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 2 cut(s) 166, 1073
XagI CCTNNNNNAGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 2 cut(s) 365, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.