Rh7DG059300

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008993
Physical Location & Seq
Forward (+)
4517917 .. 4518512
596 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG059300.1

Sequence Viewer

Length: 471 bp
ATGTGTGGGATGAATCTGGCATTCAACCGTGAGTTGATCGGCCCTGCCATGTACTTTGGACTCATGGGCGATGGGCAGCCAATTGGGCGCTACGACGATATGTGGGCTGGATGGTGCATGAAGGTTATTTGTGACCACTTGGGGCTAGGAGTGAAGACGGGATTGCCCTACATCTGGCATAGCAAAGCCAGCAACCCATTTGTTAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAGGAAGAGTTGATCCCATTTTTCCAATCTGCTACGCTTCCCAAGGATTGCACCACTGTTCAGGCCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCGAAACTTGGTAAGGTTGATGACTACTTCAACAAGCTTGCAGATGCCATGGTTACATGGATCGAAGCATGGGATGAGTTGAACCCGGCTGGGGGGAAAGCTGTTGAGCTCAACGGACCTGCAAAATCTTCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

156

Amino Acids

17.48

Weight (kDa)

6.71

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 1 - 139 7.2e-65 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 460
AclWI GGATC 2 cut(s) 244, 401
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 4 cut(s) 174, 214, 424, 425
AflII CTTAAG 1 cut(s) 209
AgsI TTSAA 4 cut(s) 25, 314, 364, 415
AluBI AGCT 3 cut(s) 370, 434, 442
AluI AGCT 3 cut(s) 370, 434, 442
Alw21I GWGCWC 1 cut(s) 444
AlwI GGATC 2 cut(s) 244, 401
AoxI GGCC 2 cut(s) 40, 300
ApeKI GCWGC 1 cut(s) 76
AspLEI GCGC 1 cut(s) 90
AspS9I GGNCC 2 cut(s) 41, 449
AsuC2I CCSGG 1 cut(s) 419
AsuII TTCGAA 1 cut(s) 464
AvaII GGWCC 1 cut(s) 449
BanII GRGCYC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 161
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 1 cut(s) 88
BccI CCATC 2 cut(s) 65, 105
BcgI CGANNNNNNTGC 1 cut(s) 444
BcnI CCSGG 1 cut(s) 419
BfaI CTAG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 91
BfrI CTTAAG 1 cut(s) 209
BfuAI ACCTGC 1 cut(s) 460
BglI GCCNNNNNGGC 1 cut(s) 85
BisI GCNGC 1 cut(s) 77
BlsI GCNGC 1 cut(s) 78
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 2 cut(s) 41, 449
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 161
Bpu14I TTCGAA 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 419
BsaJI CCNNGG 2 cut(s) 279, 381
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 214, 424, 425
Bse1I ACTGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 279, 381
BseGI GGATG 3 cut(s) 15, 116, 412
BseLI CCNNNNNNNGG 4 cut(s) 174, 214, 424, 425
BseNI ACTGG 1 cut(s) 239
BseXI GCAGC 1 cut(s) 88
BseYI CCCAGC 1 cut(s) 422
BshFI GGCC 2 cut(s) 42, 302
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 1 cut(s) 419
BslI CCNNNNNNNGG 4 cut(s) 174, 214, 424, 425
BsmI GAATGC 1 cut(s) 20
BsnI GGCC 2 cut(s) 42, 302
Bsp119I TTCGAA 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 444
Bsp143I GATC 3 cut(s) 36, 249, 393
Bsp19I CCATGG 1 cut(s) 381
BspANI GGCC 2 cut(s) 42, 302
BspMI ACCTGC 1 cut(s) 460
BspPI GGATC 2 cut(s) 244, 401
BspT104I TTCGAA 1 cut(s) 464
BspTI CTTAAG 1 cut(s) 209
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 279, 381
BssMI GATC 3 cut(s) 36, 249, 393
BssT1I CCWWGG 2 cut(s) 279, 381
Bst4CI ACNGT 2 cut(s) 29, 295
Bst6I CTCTTC 1 cut(s) 237
BstAFI CTTAAG 1 cut(s) 209
BstBI TTCGAA 1 cut(s) 464
BstC8I GCNNGC 3 cut(s) 190, 304, 372
BstDSI CCRYGG 1 cut(s) 381
BstENI CCTNNNNNAGG 1 cut(s) 212
BstF5I GGATG 3 cut(s) 15, 116, 412
BstH2I RGCGCY 1 cut(s) 91
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 3 cut(s) 39, 252, 396
BstMBI GATC 3 cut(s) 36, 249, 393
BstMWI GCNNNNNNNGC 2 cut(s) 85, 189
BstSCI CCNGG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 42, 302
BtgI CCRYGG 1 cut(s) 381
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 3 cut(s) 15, 116, 412
BtsIMutI CAGTG 1 cut(s) 291
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 3 cut(s) 190, 304, 372
CfoI GCGC 1 cut(s) 90
Cfr13I GGNCC 2 cut(s) 41, 449
Csp6I GTAC 1 cut(s) 52
CviAII CATG 6 cut(s) 49, 64, 118, 382, 390, 402
CviQI GTAC 1 cut(s) 52
DpnI GATC 3 cut(s) 38, 251, 395
DpnII GATC 3 cut(s) 36, 249, 393
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 279, 381
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 444
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 442
EcoNI CCTNNNNNAGG 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 279, 381
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 2 cut(s) 279, 381
FaeI CATG 6 cut(s) 52, 67, 121, 385, 393, 405
FaiI YATR 8 cut(s) 50, 65, 101, 119, 180, 383, 391, 403
FatI CATG 6 cut(s) 48, 63, 117, 381, 389, 401
Fnu4HI GCNGC 1 cut(s) 77
FokI GGATG 3 cut(s) 22, 123, 419
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 1 cut(s) 77
FspBI CTAG 1 cut(s) 146
GlaI GCGC 1 cut(s) 89
GluI GCNGC 1 cut(s) 77
GsaI CCCAGC 1 cut(s) 426
HaeII RGCGCY 1 cut(s) 91
HaeIII GGCC 2 cut(s) 42, 302
HapII CCGG 1 cut(s) 419
HhaI GCGC 1 cut(s) 90
Hin1II CATG 6 cut(s) 52, 67, 121, 385, 393, 405
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 3 cut(s) 13, 60, 228
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 419
Hpy166II GTNNAC 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 98
HpyAV CCTTC 2 cut(s) 115, 208
HpyCH4III ACNGT 2 cut(s) 29, 295
HpyCH4V TGCA 4 cut(s) 117, 288, 374, 455
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 189
Hsp92II CATG 6 cut(s) 52, 67, 121, 385, 393, 405
HspAI GCGC 1 cut(s) 88
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 3 cut(s) 36, 249, 393
Lsp1109I GCAGC 1 cut(s) 88
LweI GCATC 1 cut(s) 367
MaeI CTAG 1 cut(s) 146
MaeIII GTNAC 2 cut(s) 131, 385
MalI GATC 3 cut(s) 38, 251, 395
MboI GATC 3 cut(s) 36, 249, 393
MboII GAAGA 3 cut(s) 166, 254, 453
MfeI CAATTG 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 1 cut(s) 81
MlyI GAGTC 1 cut(s) 54
MseI TTAA 2 cut(s) 204, 210
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 85, 189
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 1 cut(s) 381
NdeII GATC 3 cut(s) 36, 249, 393
NlaIII CATG 6 cut(s) 52, 67, 121, 385, 393, 405
NmuCI GTSAC 1 cut(s) 131
NspV TTCGAA 1 cut(s) 464
PceI AGGCCT 1 cut(s) 302
PctI GAATGC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 13, 228
PkrI GCNGC 1 cut(s) 78
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 422
PspPI GGNCC 2 cut(s) 41, 449
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SacI GAGCTC 1 cut(s) 444
SaqAI TTAA 2 cut(s) 204, 210
SatI GCNGC 1 cut(s) 77
Sau3AI GATC 3 cut(s) 36, 249, 393
Sau96I GGNCC 2 cut(s) 41, 449
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 1 cut(s) 419
SduI GDGCHC 1 cut(s) 444
SetI ASST 8 cut(s) 126, 210, 312, 351, 372, 436, 444, 454
SfaNI GCATC 1 cut(s) 367
SfuI TTCGAA 1 cut(s) 464
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 1 cut(s) 81
SseBI AGGCCT 1 cut(s) 302
SspMI CTAG 1 cut(s) 146
SstI GAGCTC 1 cut(s) 444
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 2 cut(s) 279, 381
TaaI ACNGT 2 cut(s) 29, 295
TaqI TCGA 2 cut(s) 396, 464
TasI AATT 1 cut(s) 81
TatI WGTACW 1 cut(s) 51
TfiI GAWTC 2 cut(s) 13, 228
Tru1I TTAA 2 cut(s) 204, 210
Tru9I TTAA 2 cut(s) 204, 210
TscAI CASTG 1 cut(s) 298
TseFI GTSAC 1 cut(s) 131
TseI GCWGC 1 cut(s) 76
Tsp45I GTSAC 1 cut(s) 131
TspDTI ATGAA 2 cut(s) 26, 134
TspGWI ACGGA 1 cut(s) 462
TspRI CASTG 1 cut(s) 298
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 212
XspI CTAG 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.