FvH4_5g13530

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0008993
Physical Location & Seq
Reverse (-)
7648384 .. 7651057
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13530.t1

Sequence Viewer

Length: 1095 bp
ATGGCCGAGTACAGCAAGGTCGCCCCGACGCCGCTGCTGAAGGATGAGCTGGACATCGTGATCCCCACCATCCGAAACCTGGACTTCTTGGAAATGTGGAGGCCCTTCTTCCAGCAGTACCATCTCATCATCGTCCAGGACGGCGATCCCACCAAGATCATCAAGGTCCCCGAGGGCTTCGATTACGAGCTCTACAACCGTAACGACATCAACCGTATTCTCGGCCCCAAAGCCTCCTGCATCTCCTTCAAGGACTCCGCCTGCCGCTGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTCGTCGCTAAAGATCCTACTGGAAAAGAGATCAATGCACTAGAGCAGCACATCAAGAACCTTCTGTGCCCATCGACTCCGTTTTTCTTTAACACCTTGTACGACCCGTACCGTGAAGGTACAGATTTCGTTCGTGGTTACCCTTTTAGTCTTCGTGAAGGTGTGCCGACCGCTGTTTCTCACGGCCTCTGGCTCAATATTCCGGATTATGATGCACCCACCCAGCTTGTCAAGCCTCTTGAGAGGAACACAAGGTTTGTGGATGCAGTCTTGACTATCCCCAAGGGAACTCTCTTTCCCATGTGTGGGATGAATTTGGCTTTCAACCGTGAGTTGATCGGCCCTGCTATGTACTTTGGACTCATGGGCGATGGTCAGCCAATTGGTCGCTACGACGATATGTGGGCTGGATGGTGCATGAAGGTCATTTGTGACCACTTGGGCCTAGGAGTGAAGACGGGACTGCCCTACATCTGGCATAGCAAAGCCAGCAACCCCTTTGTTAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAGGAAGAGTTGATCCCATTTTTCCAATCTGCTACACTCCCCAAGGATTGCACCACTGTTCAGGCCTGCTACCTTGAACTCTCCAAGCAGGTGAAGGCGAAACTCGGTAAGGTTGATGACTACTTCATCAAGCTTGCAGATGCCATGGTCACGTGGATCGAAGCATGGGATGAGTTGAACCCGGCTGGAGGGAAAGCTGTTGAGCTCAATGGGCCTTCAAAATCTACAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

365

Amino Acids

41.43

Weight (kDa)

6.03

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 347 2e-179 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 940
Acc36I ACCTGC 1 cut(s) 940
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 4 cut(s) 32, 258, 265, 495
AclWI GGATC 5 cut(s) 55, 140, 332, 868, 1025
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 1 cut(s) 59
AcvI CACGTG 1 cut(s) 1014
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 7 cut(s) 11, 119, 299, 425, 434, 445, 677
AfiI CCNNNNNNNGG 6 cut(s) 79, 629, 630, 798, 838, 1049
AflII CTTAAG 1 cut(s) 833
AgsI TTSAA 5 cut(s) 250, 649, 938, 1039, 1080
AjnI CCWGG 2 cut(s) 78, 135
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 6 cut(s) 49, 190, 550, 994, 1058, 1066
AluI AGCT 6 cut(s) 49, 190, 550, 994, 1058, 1066
Alw21I GWGCWC 2 cut(s) 192, 1068
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 5 cut(s) 55, 140, 332, 868, 1025
Ama87I CYCGRG 1 cut(s) 170
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 8 cut(s) 3, 101, 223, 508, 664, 766, 924, 1073
ApeKI GCWGC 3 cut(s) 34, 267, 370
ApoI RAATTY 1 cut(s) 637
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspA2I CCTAGG 1 cut(s) 769
AspS9I GGNCC 6 cut(s) 102, 166, 224, 665, 766, 1073
AsuC2I CCSGG 1 cut(s) 1043
AsuHPI GGTGA 2 cut(s) 298, 964
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 166
AvrII CCTAGG 1 cut(s) 769
BaeGI GKGCMC 1 cut(s) 395
BanII GRGCYC 2 cut(s) 192, 1068
BbrPI CACGTG 1 cut(s) 1014
BbsI GAAGAC 2 cut(s) 467, 785
Bbv12I GWGCWC 2 cut(s) 192, 1068
BbvI GCAGC 3 cut(s) 21, 254, 382
BccI CCATC 6 cut(s) 77, 129, 317, 403, 689, 729
BceAI ACGGC 2 cut(s) 157, 523
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 2 cut(s) 80, 137
BcnI CCSGG 1 cut(s) 1043
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 2 cut(s) 365, 770
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 940
BisI GCNGC 5 cut(s) 32, 35, 265, 268, 371
BlnI CCTAGG 1 cut(s) 769
BlsI GCNGC 5 cut(s) 33, 36, 266, 269, 372
Bme1390I CCNGG 3 cut(s) 80, 137, 1043
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 6 cut(s) 102, 166, 224, 665, 766, 1073
BmiI GGNNCC 2 cut(s) 168, 226
BmrFI CCNGG 3 cut(s) 80, 137, 1043
BmsI GCATC 4 cut(s) 249, 526, 577, 991
BpiI GAAGAC 2 cut(s) 467, 785
BpmI CTGGAG 1 cut(s) 1068
BpuEI CTTGAG 1 cut(s) 584
BpuMI CCSGG 1 cut(s) 1043
BsaAI YACGTR 1 cut(s) 1014
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 5 cut(s) 171, 606, 769, 903, 1005
BsaWI WCCGGW 1 cut(s) 526
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 6 cut(s) 79, 629, 630, 798, 838, 1049
Bse1I ACTGG 2 cut(s) 349, 863
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 2 cut(s) 80, 137
BseDI CCNNGG 5 cut(s) 171, 606, 769, 903, 1005
BseGI GGATG 6 cut(s) 49, 69, 592, 639, 740, 1036
BseLI CCNNNNNNNGG 6 cut(s) 79, 629, 630, 798, 838, 1049
BseNI ACTGG 2 cut(s) 349, 863
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 21, 254, 382
BseYI CCCAGC 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 495
BshFI GGCC 8 cut(s) 5, 103, 225, 510, 666, 768, 926, 1075
BsiEI CGRYCG 1 cut(s) 495
BsiHKAI GWGCWC 2 cut(s) 192, 1068
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 2 cut(s) 527, 1043
BslFI GGGAC 2 cut(s) 152, 798
BslI CCNNNNNNNGG 6 cut(s) 79, 629, 630, 798, 838, 1049
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 2 cut(s) 152, 798
BsnI GGCC 8 cut(s) 5, 103, 225, 510, 666, 768, 926, 1075
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 3 cut(s) 192, 395, 1068
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 4 cut(s) 32, 258, 265, 495
BspANI GGCC 8 cut(s) 5, 103, 225, 510, 666, 768, 926, 1075
BspEI TCCGGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 168, 226
BspMI ACCTGC 1 cut(s) 940
BspPI GGATC 5 cut(s) 55, 140, 332, 868, 1025
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 2 cut(s) 349, 863
BssECI CCNNGG 5 cut(s) 171, 606, 769, 903, 1005
BssMI GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 4 cut(s) 606, 769, 903, 1005
Bst2UI CCWGG 2 cut(s) 80, 137
Bst4CI ACNGT 5 cut(s) 200, 215, 437, 653, 919
Bst6I CTCTTC 1 cut(s) 861
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 1014
BstC8I GCNNGC 4 cut(s) 262, 814, 928, 996
BstDSI CCRYGG 1 cut(s) 1005
BstEII GGTNACC 1 cut(s) 461
BstENI CCTNNNNNAGG 1 cut(s) 836
BstF5I GGATG 6 cut(s) 49, 69, 592, 639, 740, 1036
BstKTI GATC 8 cut(s) 63, 148, 159, 340, 357, 663, 876, 1020
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
BstMCI CGRYCG 1 cut(s) 495
BstMWI GCNNNNNNNGC 4 cut(s) 273, 556, 813, 1072
BstNI CCWGG 2 cut(s) 80, 137
BstPI GGTNACC 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 78, 135, 1041
BstSLI GKGCMC 1 cut(s) 395
BstV1I GCAGC 3 cut(s) 21, 254, 382
BstV2I GAAGAC 2 cut(s) 467, 785
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 8 cut(s) 5, 103, 225, 510, 666, 768, 926, 1075
BtgI CCRYGG 1 cut(s) 1005
BtgZI GCGATG 1 cut(s) 708
BtsCI GGATG 6 cut(s) 49, 69, 592, 639, 740, 1036
BtsIMutI CAGTG 1 cut(s) 915
BveI ACCTGC 1 cut(s) 940
Cac8I GCNNGC 4 cut(s) 262, 814, 928, 996
Cfr13I GGNCC 6 cut(s) 102, 166, 224, 665, 766, 1073
CseI GACGC 1 cut(s) 37
Csp6I GTAC 7 cut(s) 10, 118, 298, 424, 433, 444, 676
CspCI CAANNNNNGTGG 4 cut(s) 532, 564, 567, 599
CviAII CATG 6 cut(s) 280, 625, 688, 742, 1006, 1026
CviQI GTAC 7 cut(s) 10, 118, 298, 424, 433, 444, 676
DpnI GATC 8 cut(s) 62, 147, 158, 339, 356, 662, 875, 1019
DpnII GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
EciI GGCGGA 1 cut(s) 247
Ecl136II GAGCTC 2 cut(s) 190, 1066
Eco130I CCWWGG 4 cut(s) 606, 769, 903, 1005
Eco147I AGGCCT 1 cut(s) 926
Eco24I GRGCYC 2 cut(s) 192, 1068
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 166
Eco53kI GAGCTC 2 cut(s) 190, 1066
Eco57I CTGAAG 1 cut(s) 59
Eco72I CACGTG 1 cut(s) 1014
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 461
EcoICRI GAGCTC 2 cut(s) 190, 1066
EcoNI CCTNNNNNAGG 1 cut(s) 836
EcoO109I RGGNCCY 2 cut(s) 102, 166
EcoO65I GGTNACC 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 78, 135
EcoT14I CCWWGG 4 cut(s) 606, 769, 903, 1005
EcoT38I GRGCYC 2 cut(s) 192, 1068
ErhI CCWWGG 4 cut(s) 606, 769, 903, 1005
FaeI CATG 6 cut(s) 283, 628, 691, 745, 1009, 1029
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 2 cut(s) 152, 798
FatI CATG 6 cut(s) 279, 624, 687, 741, 1005, 1025
Fnu4HI GCNGC 5 cut(s) 32, 35, 265, 268, 371
FokI GGATG 6 cut(s) 56, 56, 599, 646, 747, 1043
FriOI GRGCYC 2 cut(s) 192, 1068
Fsp4HI GCNGC 5 cut(s) 32, 35, 265, 268, 371
FspBI CTAG 2 cut(s) 365, 770
GluI GCNGC 5 cut(s) 32, 35, 265, 268, 371
GsaI CCCAGC 1 cut(s) 550
GsuI CTGGAG 1 cut(s) 1068
HaeIII GGCC 8 cut(s) 5, 103, 225, 510, 666, 768, 926, 1075
HapII CCGG 2 cut(s) 527, 1043
HgaI GACGC 1 cut(s) 37
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 6 cut(s) 283, 628, 691, 745, 1009, 1029
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 4 cut(s) 254, 400, 684, 852
HpaI GTTAAC 1 cut(s) 829
HpaII CCGG 2 cut(s) 527, 1043
HphI GGTGA 2 cut(s) 298, 964
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 6 cut(s) 58, 379, 479, 527, 563, 595
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 5 cut(s) 31, 317, 320, 332, 722
HpyCH4III ACNGT 5 cut(s) 200, 215, 437, 653, 919
HpyCH4IV ACGT 1 cut(s) 1013
HpyCH4V TGCA 7 cut(s) 240, 362, 539, 590, 741, 912, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 273, 556, 813, 1072
HpySE526I ACGT 1 cut(s) 1013
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 6 cut(s) 283, 628, 691, 745, 1009, 1029
Kpn2I TCCGGA 1 cut(s) 526
KspAI GTTAAC 1 cut(s) 829
Kzo9I GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
Lsp1109I GCAGC 3 cut(s) 21, 254, 382
LweI GCATC 4 cut(s) 249, 526, 577, 991
MaeI CTAG 2 cut(s) 365, 770
MaeII ACGT 1 cut(s) 1013
MaeIII GTNAC 4 cut(s) 200, 461, 755, 1009
MalI GATC 8 cut(s) 62, 147, 158, 339, 356, 662, 875, 1019
MboI GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
MboII GAAGA 6 cut(s) 100, 295, 304, 467, 790, 878
MfeI CAATTG 1 cut(s) 705
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 3 cut(s) 192, 395, 1068
MluCI AATT 2 cut(s) 637, 705
MlyI GAGTC 3 cut(s) 248, 394, 678
MnlI CCTC 7 cut(s) 93, 166, 244, 521, 561, 570, 1043
MroI TCCGGA 1 cut(s) 526
MseI TTAA 3 cut(s) 414, 828, 834
MspA1I CMGCKG 3 cut(s) 34, 267, 497
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 2 cut(s) 527, 1043
MspR9I CCNGG 3 cut(s) 80, 137, 1043
MunI CAATTG 1 cut(s) 705
MvaI CCWGG 2 cut(s) 80, 137
MwoI GCNNNNNNNGC 4 cut(s) 273, 556, 813, 1072
NciI CCSGG 1 cut(s) 1043
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
NlaIII CATG 6 cut(s) 283, 628, 691, 745, 1009, 1029
NlaIV GGNNCC 2 cut(s) 168, 226
NmeAIII GCCGAG 2 cut(s) 31, 201
NmuCI GTSAC 2 cut(s) 755, 1009
PaqCI CACCTGC 1 cut(s) 940
PceI AGGCCT 1 cut(s) 926
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 1 cut(s) 852
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 5 cut(s) 33, 36, 266, 269, 372
PleI GAGTC 3 cut(s) 248, 394, 678
PmaCI CACGTG 1 cut(s) 1014
PmlI CACGTG 1 cut(s) 1014
PpsI GAGTC 3 cut(s) 248, 394, 678
Ppu21I YACGTR 1 cut(s) 1014
PpuMI RGGWCCY 1 cut(s) 166
Psp124BI GAGCTC 2 cut(s) 192, 1068
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 2 cut(s) 78, 135
PspCI CACGTG 1 cut(s) 1014
PspEI GGTNACC 1 cut(s) 461
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 2 cut(s) 78, 135
PspN4I GGNNCC 2 cut(s) 168, 226
PspPI GGNCC 6 cut(s) 102, 166, 224, 665, 766, 1073
PspPPI RGGWCCY 1 cut(s) 166
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 7 cut(s) 11, 119, 299, 425, 434, 445, 677
RsaNI GTAC 7 cut(s) 10, 118, 298, 424, 433, 444, 676
SacI GAGCTC 2 cut(s) 192, 1068
SaqAI TTAA 3 cut(s) 414, 828, 834
SatI GCNGC 5 cut(s) 32, 35, 265, 268, 371
Sau3AI GATC 8 cut(s) 60, 145, 156, 337, 354, 660, 873, 1017
Sau96I GGNCC 6 cut(s) 102, 166, 224, 665, 766, 1073
SchI GAGTC 3 cut(s) 248, 394, 678
ScrFI CCNGG 3 cut(s) 80, 137, 1043
SduI GDGCHC 3 cut(s) 192, 395, 1068
SfaNI GCATC 4 cut(s) 249, 526, 577, 991
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 2 cut(s) 563, 833
SmoI CTYRAG 2 cut(s) 563, 833
Sse9I AATT 2 cut(s) 637, 705
SseBI AGGCCT 1 cut(s) 926
SsiI CCGC 4 cut(s) 32, 258, 265, 495
SspI AATATT 1 cut(s) 523
SspMI CTAG 2 cut(s) 365, 770
SstI GAGCTC 2 cut(s) 192, 1068
StuI AGGCCT 1 cut(s) 926
StyD4I CCNGG 3 cut(s) 78, 135, 1041
StyI CCWWGG 4 cut(s) 606, 769, 903, 1005
TaaI ACNGT 5 cut(s) 200, 215, 437, 653, 919
TaiI ACGT 1 cut(s) 1016
TaqI TCGA 4 cut(s) 180, 312, 398, 1020
TasI AATT 2 cut(s) 637, 705
TatI WGTACW 3 cut(s) 9, 297, 675
TauI GCSGC 2 cut(s) 34, 267
TfiI GAWTC 1 cut(s) 852
Tru1I TTAA 3 cut(s) 414, 828, 834
Tru9I TTAA 3 cut(s) 414, 828, 834
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 2 cut(s) 755, 1009
TseI GCWGC 3 cut(s) 34, 267, 370
Tsp45I GTSAC 2 cut(s) 755, 1009
TspDTI ATGAA 3 cut(s) 650, 758, 976
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 922
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 1 cut(s) 166
XagI CCTNNNNNAGG 1 cut(s) 836
XapI RAATTY 1 cut(s) 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmaJI CCTAGG 1 cut(s) 769
XspI CTAG 2 cut(s) 365, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.