pycom14g17810

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008993
Physical Location & Seq
Forward (+)
19623546 .. 19625683
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17810.1

Sequence Viewer

Length: 1089 bp
ATGGCGCAGTACAGCAAGGTCGCCCCGACCCCTCTGCTGAAAGACGAGCTTGATATCGTCATCCCCACCATCCGAAACCTCGATTTCCTCGAGATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCGTCGAAGGTGATCAAGGTCCCGGAAGGCTTCGACTACGAGCTCTACAACCGCAACGACATCAACCGGATTCTGGGTCCCAAGGCGTCCTGCATCTCCTTCAAGGACTCTGCTTGCAGATGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTTGTTGCTAAGGATCCTTCTGGCAAAGATATCAATGCTCTTGAGCAGCATATCAAGAACCTTCTGTCTCCATCAACTCCATTTTTCTTCAACACCCTGTACGACCCATACCGTGAAGGTGCAGATTTCGTTCGTGGATACCCCTTCAGTCTGCGTGAAGGTATCCCAACTGCTGTTTCTCACGGCCTCTGGCTCAACATCCCGGATTATGATGCTCCCACCCAGCTTGTTAAGCCTCTAGAGAGAAATACCAGGTTTGTGGATGCAGTCCTGACCATTCCCAAGGGAACTCTCTTTCCCATGTGCGGCATGAATCTGGCATTCAACCGTGAGTTGATTGGACCTGCTATGTACTTCGGACTCATGGGTGATGGTCAGCCAATCGGGCGCTACGACGACATGTGGGCTGGCTGGTGCATGAAGGTTATCTGTGACCACTTGGGGTTGGGAGTTAAGACAGGATTGCCCTACATCTGGCATAGCAAGGCAAGCAACCCATTTGTGAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAAGAGGAGTTGATCCCCTTTTTCCAGTCCGCCACCCTCCCAAAGGATTGCACCACCGTCCAGGGCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCCAAACTCGGCAAGGTCGATGACTACTTCATCAAGCTTGCAGATGCCATGATCACATGGGTCGAAGCATGGGACGAGTTGAACCCAGTCGAAGGAAAGGCTGTTGAGGCCAACGGAGTTGTTGCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

363

Amino Acids

41.16

Weight (kDa)

5.83

Isoelectric Point (pI)

28.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 347 4.6e-179 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AciI CCGC 3 cut(s) 199, 630, 891
AclWI GGATC 3 cut(s) 332, 345, 868
AcuI CTGAAG 1 cut(s) 454
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 5 cut(s) 11, 119, 299, 425, 677
AfiI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 5 cut(s) 250, 415, 649, 938, 1039
AjnI CCWGG 3 cut(s) 135, 575, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AloI GAACNNNNNNTCC 2 cut(s) 604, 636
AluBI AGCT 4 cut(s) 49, 190, 550, 994
AluI AGCT 4 cut(s) 49, 190, 550, 994
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 2 cut(s) 289, 396
AlwI GGATC 3 cut(s) 332, 345, 868
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 4 cut(s) 101, 508, 957, 1065
ApeKI GCWGC 2 cut(s) 370, 927
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 2 cut(s) 7, 714
AspS9I GGNCC 4 cut(s) 102, 166, 224, 665
AsuC2I CCSGG 2 cut(s) 170, 527
AsuHPI GGTGA 3 cut(s) 169, 298, 704
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 3 cut(s) 166, 224, 665
BamHI GGATCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 382, 914
BccI CCATC 4 cut(s) 77, 317, 403, 689
BceAI ACGGC 2 cut(s) 157, 523
BcgI CGANNNNNNTGC 4 cut(s) 16, 50, 253, 287
BciT130I CCWGG 3 cut(s) 137, 577, 923
BciVI GTATCC 2 cut(s) 455, 497
BclI TGATCA 2 cut(s) 159, 1008
BcnI CCSGG 2 cut(s) 170, 527
BcoDI GTCTC 2 cut(s) 289, 396
BfaI CTAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 715
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 2 cut(s) 455, 497
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 3 cut(s) 371, 631, 928
BlsI GCNGC 3 cut(s) 372, 632, 929
Bme1390I CCNGG 5 cut(s) 137, 170, 527, 577, 923
Bme18I GGWCC 3 cut(s) 166, 224, 665
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 4 cut(s) 102, 166, 224, 665
BmiI GGNNCC 4 cut(s) 168, 225, 226, 339
BmrFI CCNGG 5 cut(s) 137, 170, 527, 577, 923
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 5 cut(s) 249, 257, 526, 577, 991
BmuI ACTGGG 1 cut(s) 1037
Bpu10I CCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 2 cut(s) 170, 527
BsaHI GRCGYC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 228, 606, 922
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
Bse1I ACTGG 3 cut(s) 863, 886, 1043
BseBI CCWGG 3 cut(s) 137, 577, 923
BseDI CCNNGG 3 cut(s) 228, 606, 922
BseGI GGATG 4 cut(s) 60, 69, 522, 592
BseLI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
BseNI ACTGG 3 cut(s) 863, 886, 1043
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 2 cut(s) 382, 914
BseYI CCCAGC 1 cut(s) 546
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 4 cut(s) 103, 510, 959, 1067
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 3 cut(s) 170, 214, 527
BslFI GGGAC 3 cut(s) 152, 210, 1043
BslI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
BsmAI GTCTC 2 cut(s) 289, 396
BsmFI GGGAC 3 cut(s) 152, 210, 1043
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 4 cut(s) 103, 510, 959, 1067
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 4 cut(s) 159, 337, 873, 1008
BspACI CCGC 3 cut(s) 199, 630, 891
BspANI GGCC 4 cut(s) 103, 510, 959, 1067
BspLI GGNNCC 4 cut(s) 168, 225, 226, 339
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 3 cut(s) 332, 345, 868
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 3 cut(s) 863, 886, 1043
BssECI CCNNGG 3 cut(s) 228, 606, 922
BssMI GATC 4 cut(s) 159, 337, 873, 1008
BssNI GRCGYC 1 cut(s) 233
BssT1I CCWWGG 2 cut(s) 228, 606
Bst2UI CCWGG 3 cut(s) 137, 577, 923
Bst4CI ACNGT 3 cut(s) 437, 653, 919
BstACI GRCGYC 1 cut(s) 233
BstAFI CTTAAG 1 cut(s) 833
BstC8I GCNNGC 4 cut(s) 262, 733, 814, 996
BstDEI CTNAG 1 cut(s) 333
BstENI CCTNNNNNAGG 2 cut(s) 836, 902
BstF5I GGATG 4 cut(s) 60, 69, 522, 592
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 2 cut(s) 7, 714
BstKTI GATC 4 cut(s) 162, 340, 876, 1011
BstMAI GTCTC 2 cut(s) 289, 396
BstMBI GATC 4 cut(s) 159, 337, 873, 1008
BstMWI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
BstNI CCWGG 3 cut(s) 137, 577, 923
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 5 cut(s) 135, 168, 525, 575, 921
BstV1I GCAGC 2 cut(s) 382, 914
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 337
BsuI GTATCC 2 cut(s) 455, 497
BsuRI GGCC 4 cut(s) 103, 510, 959, 1067
BtsCI GGATG 4 cut(s) 60, 69, 522, 592
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 4 cut(s) 262, 733, 814, 996
CfoI GCGC 2 cut(s) 7, 714
Cfr13I GGNCC 4 cut(s) 102, 166, 224, 665
CseI GACGC 1 cut(s) 222
CsiI ACCWGGT 1 cut(s) 575
Csp6I GTAC 5 cut(s) 10, 118, 298, 424, 676
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 9 cut(s) 280, 625, 634, 688, 724, 742, 1006, 1014, 1026
CviQI GTAC 5 cut(s) 10, 118, 298, 424, 676
DdeI CTNAG 1 cut(s) 333
DpnI GATC 4 cut(s) 161, 339, 875, 1010
DpnII GATC 4 cut(s) 159, 337, 873, 1008
EciI GGCGGA 1 cut(s) 880
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 2 cut(s) 228, 606
Eco24I GRGCYC 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 289
Eco32I GATATC 2 cut(s) 55, 355
Eco47I GGWCC 3 cut(s) 166, 224, 665
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 454
Eco88I CYCGRG 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 190
EcoNI CCTNNNNNAGG 2 cut(s) 836, 902
EcoO109I RGGNCCY 3 cut(s) 102, 166, 224
EcoRII CCWGG 3 cut(s) 135, 575, 921
EcoRV GATATC 2 cut(s) 55, 355
EcoT14I CCWWGG 2 cut(s) 228, 606
EcoT38I GRGCYC 1 cut(s) 192
ErhI CCWWGG 2 cut(s) 228, 606
FaeI CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
FalI AAGNNNNNCTT 4 cut(s) 33, 65, 287, 319
FaqI GGGAC 3 cut(s) 152, 210, 1043
FatI CATG 9 cut(s) 279, 624, 633, 687, 723, 741, 1005, 1013, 1025
FbaI TGATCA 2 cut(s) 159, 1008
Fnu4HI GCNGC 3 cut(s) 371, 631, 928
FokI GGATG 4 cut(s) 47, 56, 509, 599
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 3 cut(s) 371, 631, 928
FspBI CTAG 1 cut(s) 563
GlaI GCGC 2 cut(s) 6, 713
GluI GCNGC 3 cut(s) 371, 631, 928
GsaI CCCAGC 1 cut(s) 550
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 4 cut(s) 103, 510, 959, 1067
HapII CCGG 3 cut(s) 170, 214, 527
HgaI GACGC 1 cut(s) 222
HhaI GCGC 2 cut(s) 7, 714
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
Hin6I GCGC 2 cut(s) 5, 712
HinP1I GCGC 2 cut(s) 5, 712
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 5 cut(s) 217, 254, 637, 684, 852
HpaII CCGG 3 cut(s) 170, 214, 527
HphI GGTGA 3 cut(s) 169, 298, 704
Hpy166II GTNNAC 1 cut(s) 829
Hpy188I TCNGA 2 cut(s) 74, 683
Hpy188III TCNNGA 5 cut(s) 91, 365, 379, 563, 595
Hpy8I GTNNAC 1 cut(s) 829
Hpy99I CGWCG 4 cut(s) 154, 317, 320, 722
HpyCH4III ACNGT 3 cut(s) 437, 653, 919
HpyCH4V TGCA 7 cut(s) 240, 264, 446, 590, 741, 912, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
HpyF3I CTNAG 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
HspAI GCGC 2 cut(s) 5, 712
KflI GGGWCCC 1 cut(s) 224
Ksp22I TGATCA 2 cut(s) 159, 1008
Kzo9I GATC 4 cut(s) 159, 337, 873, 1008
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 2 cut(s) 382, 914
LweI GCATC 5 cut(s) 249, 257, 526, 577, 991
MabI ACCWGGT 1 cut(s) 575
MaeI CTAG 1 cut(s) 563
MaeIII GTNAC 1 cut(s) 755
MalI GATC 4 cut(s) 161, 339, 875, 1010
MboI GATC 4 cut(s) 159, 337, 873, 1008
MboII GAAGA 4 cut(s) 100, 295, 304, 403
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 192
MlyI GAGTC 2 cut(s) 248, 678
MseI TTAA 3 cut(s) 555, 777, 834
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 3 cut(s) 170, 214, 527
MspR9I CCNGG 5 cut(s) 137, 170, 527, 577, 923
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 3 cut(s) 137, 577, 923
MwoI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
NciI CCSGG 2 cut(s) 170, 527
NdeII GATC 4 cut(s) 159, 337, 873, 1008
NlaIII CATG 9 cut(s) 283, 628, 637, 691, 727, 745, 1009, 1017, 1029
NlaIV GGNNCC 4 cut(s) 168, 225, 226, 339
NmeAIII GCCGAG 1 cut(s) 945
NmuCI GTSAC 1 cut(s) 755
NspI RCATGY 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 89
PciI ACATGT 1 cut(s) 723
PcsI WCGNNNNNNNCGW 3 cut(s) 87, 714, 972
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 217, 637, 852
PfoI TCCNGGA 3 cut(s) 135, 168, 525
PkrI GCNGC 3 cut(s) 372, 632, 929
PleI GAGTC 2 cut(s) 248, 678
PpsI GAGTC 2 cut(s) 248, 678
PpuMI RGGWCCY 2 cut(s) 166, 224
PscI ACATGT 1 cut(s) 723
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 2 cut(s) 166, 224
Psp6I CCWGG 3 cut(s) 135, 575, 921
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 3 cut(s) 135, 575, 921
PspN4I GGNNCC 4 cut(s) 168, 225, 226, 339
PspPI GGNCC 4 cut(s) 102, 166, 224, 665
PspPPI RGGWCCY 2 cut(s) 166, 224
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 5 cut(s) 11, 119, 299, 425, 677
RsaNI GTAC 5 cut(s) 10, 118, 298, 424, 676
SacI GAGCTC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 555, 777, 834
SatI GCNGC 3 cut(s) 371, 631, 928
Sau3AI GATC 4 cut(s) 159, 337, 873, 1008
Sau96I GGNCC 4 cut(s) 102, 166, 224, 665
SchI GAGTC 2 cut(s) 248, 678
ScrFI CCNGG 5 cut(s) 137, 170, 527, 577, 923
SduI GDGCHC 1 cut(s) 192
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 5 cut(s) 249, 257, 526, 577, 991
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 3 cut(s) 166, 224, 665
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 89, 365, 833
SmoI CTYRAG 3 cut(s) 89, 365, 833
SsiI CCGC 3 cut(s) 199, 630, 891
SspMI CTAG 1 cut(s) 563
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 5 cut(s) 135, 168, 525, 575, 921
StyI CCWWGG 2 cut(s) 228, 606
TaaI ACNGT 3 cut(s) 437, 653, 919
TaqI TCGA 8 cut(s) 81, 90, 152, 180, 312, 975, 1020, 1047
TatI WGTACW 3 cut(s) 9, 297, 675
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 3 cut(s) 217, 637, 852
Tru1I TTAA 3 cut(s) 555, 777, 834
Tru9I TTAA 3 cut(s) 555, 777, 834
TseFI GTSAC 1 cut(s) 755
TseI GCWGC 2 cut(s) 370, 927
Tsp45I GTSAC 1 cut(s) 755
TspDTI ATGAA 3 cut(s) 650, 758, 976
TspGWI ACGGA 1 cut(s) 1086
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 3 cut(s) 166, 224, 665
XagI CCTNNNNNAGG 2 cut(s) 836, 902
XbaI TCTAGA 1 cut(s) 562
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.