Rh7DG059200

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0008993
Physical Location & Seq
Forward (+)
4516770 .. 4517890
1121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG059200.1

Sequence Viewer

Length: 687 bp
ATGGCGGAGTACAGCAAGGTCGCGCCGACGCCGCTGCTGAAGGATGAGCTGGACATAGTGATCCCGACGATCCGAAACCTGGACTTCTTGGAAATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCCAGCAAGGTCATCAAGGTCCCCGAGGGCTTCGACTACGAGCTTTACAACCGCAACGACATTAACCGGATTCTCGGCCCCAAAGCCGCCTGTATCTCCTTCAAGGACTCCGCCTGCCGCTGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTCGTTGCGAAAGATCCCACTGGAAAGGAGATCAATGCGCTAGAGCAGCACATCAAGAACCTTCTGTGCCCGTCGACTCCATTTTTCTTTAACACCCTGTACGACCCATACCGTGAAGGTGCAGATTTTGTCCGCGGTTACCCTTTCAGTCTGCGTGAAGGTGTGCCAACCGCTGTTTCTCACGGCCTCTGGCTCAACATTCCTGATTATGATGCACCTACCCAGCTTGTTAAGCCTCTTGAGAGGAACACCAGGTACATTGTTCTGCCACGGCAATATTTTTTACAAATTGTATATTCGAGTGTAATAGACTTGCTGATTGTGTATGCCTGTGGTTTTTTAGGTTTGTGGATGCAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

228

Amino Acids

26.36

Weight (kDa)

5.65

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 195 9.6e-100 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 398
AccII CGCG 2 cut(s) 23, 459
AciI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
AclWI GGATC 3 cut(s) 55, 64, 332
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 5 cut(s) 11, 119, 299, 425, 581
AfiI CCNNNNNNNGG 2 cut(s) 79, 154
AgsI TTSAA 1 cut(s) 250
AjnI CCWGG 3 cut(s) 78, 135, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 3 cut(s) 49, 190, 550
AluI AGCT 3 cut(s) 49, 190, 550
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 3 cut(s) 55, 64, 332
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 3 cut(s) 101, 223, 508
ApeKI GCWGC 3 cut(s) 34, 267, 370
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 2 cut(s) 25, 364
AspS9I GGNCC 3 cut(s) 102, 166, 224
AsuHPI GGTGA 1 cut(s) 298
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 166
BaeGI GKGCMC 1 cut(s) 395
BbvI GCAGC 3 cut(s) 21, 254, 382
BccI CCATC 1 cut(s) 317
BceAI ACGGC 3 cut(s) 157, 523, 611
BcgI CGANNNNNNTGC 2 cut(s) 16, 50
BciT130I CCWGG 3 cut(s) 80, 137, 577
BcoDI GTCTC 1 cut(s) 289
BfaI CTAG 1 cut(s) 365
BisI GCNGC 6 cut(s) 32, 35, 234, 265, 268, 371
BlsI GCNGC 6 cut(s) 33, 36, 235, 266, 269, 372
Bme1390I CCNGG 3 cut(s) 80, 137, 577
Bme18I GGWCC 1 cut(s) 166
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 3 cut(s) 102, 166, 224
BmiI GGNNCC 2 cut(s) 168, 226
BmrFI CCNGG 3 cut(s) 80, 137, 577
BmsI GCATC 2 cut(s) 526, 666
BpuEI CTTGAG 1 cut(s) 584
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 171, 457, 593
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 154
Bse1I ACTGG 1 cut(s) 349
BseBI CCWGG 3 cut(s) 80, 137, 577
BseDI CCNNGG 3 cut(s) 171, 457, 593
BseGI GGATG 2 cut(s) 49, 681
BseLI CCNNNNNNNGG 2 cut(s) 79, 154
BseNI ACTGG 1 cut(s) 349
BseSI GKGCMC 1 cut(s) 395
BseXI GCAGC 3 cut(s) 21, 254, 382
BseYI CCCAGC 2 cut(s) 149, 546
BsgI GTGCAG 1 cut(s) 465
Bsh1236I CGCG 2 cut(s) 23, 459
BshFI GGCC 3 cut(s) 103, 225, 510
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 214
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 2 cut(s) 79, 154
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 3 cut(s) 103, 225, 510
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 1 cut(s) 395
Bsp143I GATC 4 cut(s) 60, 69, 337, 354
BspACI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
BspANI GGCC 3 cut(s) 103, 225, 510
BspFNI CGCG 2 cut(s) 23, 459
BspLI GGNNCC 2 cut(s) 168, 226
BspPI GGATC 3 cut(s) 55, 64, 332
BspTNI GGTCTC 1 cut(s) 289
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 3 cut(s) 171, 457, 593
BssMI GATC 4 cut(s) 60, 69, 337, 354
BssNI GRCGYC 1 cut(s) 29
Bst2UI CCWGG 3 cut(s) 80, 137, 577
Bst4CI ACNGT 1 cut(s) 437
BstACI GRCGYC 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 262
BstDSI CCRYGG 2 cut(s) 457, 593
BstEII GGTNACC 1 cut(s) 461
BstF5I GGATG 2 cut(s) 49, 681
BstFNI CGCG 2 cut(s) 23, 459
BstHHI GCGC 2 cut(s) 25, 364
BstKTI GATC 4 cut(s) 63, 72, 340, 357
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 4 cut(s) 60, 69, 337, 354
BstMWI GCNNNNNNNGC 5 cut(s) 31, 150, 273, 370, 556
BstNI CCWGG 3 cut(s) 80, 137, 577
BstPI GGTNACC 1 cut(s) 461
BstSCI CCNGG 3 cut(s) 78, 135, 575
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 2 cut(s) 23, 459
BstV1I GCAGC 3 cut(s) 21, 254, 382
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 3 cut(s) 103, 225, 510
BtgI CCRYGG 2 cut(s) 457, 593
BtsCI GGATG 2 cut(s) 49, 681
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 262
CfoI GCGC 2 cut(s) 25, 364
Cfr13I GGNCC 3 cut(s) 102, 166, 224
Cfr42I CCGCGG 1 cut(s) 460
CseI GACGC 1 cut(s) 37
CsiI ACCWGGT 1 cut(s) 575
Csp6I GTAC 5 cut(s) 10, 118, 298, 424, 580
CviAII CATG 1 cut(s) 280
CviQI GTAC 5 cut(s) 10, 118, 298, 424, 580
DpnI GATC 4 cut(s) 62, 71, 339, 356
DpnII GATC 4 cut(s) 60, 69, 337, 354
EciI GGCGGA 2 cut(s) 20, 247
Eco31I GGTCTC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 166
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 170
Eco91I GGTNACC 1 cut(s) 461
EcoO109I RGGNCCY 2 cut(s) 102, 166
EcoO65I GGTNACC 1 cut(s) 461
EcoRII CCWGG 3 cut(s) 78, 135, 575
FaeI CATG 1 cut(s) 283
FaiI YATR 6 cut(s) 56, 281, 433, 534, 619, 651
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 152
FatI CATG 1 cut(s) 279
FblI GTMKAC 1 cut(s) 398
Fnu4HI GCNGC 6 cut(s) 32, 35, 234, 265, 268, 371
FokI GGATG 1 cut(s) 56
Fsp4HI GCNGC 6 cut(s) 32, 35, 234, 265, 268, 371
FspBI CTAG 1 cut(s) 365
GlaI GCGC 2 cut(s) 24, 363
GluI GCNGC 6 cut(s) 32, 35, 234, 265, 268, 371
GsaI CCCAGC 2 cut(s) 153, 550
HaeIII GGCC 3 cut(s) 103, 225, 510
HapII CCGG 1 cut(s) 214
HgaI GACGC 1 cut(s) 37
HhaI GCGC 2 cut(s) 25, 364
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 1 cut(s) 283
Hin6I GCGC 2 cut(s) 23, 362
HinP1I GCGC 2 cut(s) 23, 362
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HinfI GANTC 3 cut(s) 217, 254, 400
HpaII CCGG 1 cut(s) 214
HphI GGTGA 1 cut(s) 298
Hpy166II GTNNAC 1 cut(s) 399
Hpy188I TCNGA 1 cut(s) 74
Hpy188III TCNNGA 5 cut(s) 64, 379, 527, 563, 684
Hpy8I GTNNAC 1 cut(s) 399
Hpy99I CGWCG 5 cut(s) 31, 70, 317, 320, 400
HpyAV CCTTC 6 cut(s) 34, 115, 256, 395, 434, 476
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4V TGCA 3 cut(s) 446, 539, 679
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 150, 273, 370, 556
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 1 cut(s) 283
HspAI GCGC 2 cut(s) 23, 362
KspI CCGCGG 1 cut(s) 460
Kzo9I GATC 4 cut(s) 60, 69, 337, 354
Lsp1109I GCAGC 3 cut(s) 21, 254, 382
LweI GCATC 2 cut(s) 526, 666
MabI ACCWGGT 1 cut(s) 575
MaeI CTAG 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 461
MalI GATC 4 cut(s) 62, 71, 339, 356
MboI GATC 4 cut(s) 60, 69, 337, 354
MboII GAAGA 3 cut(s) 100, 295, 304
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 1 cut(s) 395
MluCI AATT 1 cut(s) 612
MlyI GAGTC 2 cut(s) 248, 394
MnlI CCTC 6 cut(s) 93, 134, 166, 521, 561, 570
MseI TTAA 3 cut(s) 210, 414, 555
MspA1I CMGCKG 4 cut(s) 34, 267, 459, 497
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 3 cut(s) 80, 137, 577
MvaI CCWGG 3 cut(s) 80, 137, 577
MvnI CGCG 2 cut(s) 23, 459
MwoI GCNNNNNNNGC 5 cut(s) 31, 150, 273, 370, 556
NdeII GATC 4 cut(s) 60, 69, 337, 354
NlaIII CATG 1 cut(s) 283
NlaIV GGNNCC 2 cut(s) 168, 226
NmeAIII GCCGAG 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PfeI GAWTC 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 6 cut(s) 33, 36, 235, 266, 269, 372
PleI GAGTC 2 cut(s) 248, 394
PpsI GAGTC 2 cut(s) 248, 394
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 3 cut(s) 78, 135, 575
PspEI GGTNACC 1 cut(s) 461
PspFI CCCAGC 2 cut(s) 149, 546
PspGI CCWGG 3 cut(s) 78, 135, 575
PspN4I GGNNCC 2 cut(s) 168, 226
PspPI GGNCC 3 cut(s) 102, 166, 224
PspPPI RGGWCCY 1 cut(s) 166
PsrI GAACNNNNNNTAC 2 cut(s) 563, 595
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 5 cut(s) 11, 119, 299, 425, 581
RsaNI GTAC 5 cut(s) 10, 118, 298, 424, 580
SacII CCGCGG 1 cut(s) 460
SalI GTCGAC 1 cut(s) 397
SaqAI TTAA 3 cut(s) 210, 414, 555
SatI GCNGC 6 cut(s) 32, 35, 234, 265, 268, 371
Sau3AI GATC 4 cut(s) 60, 69, 337, 354
Sau96I GGNCC 3 cut(s) 102, 166, 224
SchI GAGTC 2 cut(s) 248, 394
ScrFI CCNGG 3 cut(s) 80, 137, 577
SduI GDGCHC 1 cut(s) 395
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 2 cut(s) 526, 666
Sfr303I CCGCGG 1 cut(s) 460
SgrBI CCGCGG 1 cut(s) 460
SinI GGWCC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 1 cut(s) 612
SsiI CCGC 9 cut(s) 5, 32, 199, 234, 258, 265, 457, 459, 495
SspI AATATT 1 cut(s) 602
SspMI CTAG 1 cut(s) 365
StyD4I CCNGG 3 cut(s) 78, 135, 575
TaaI ACNGT 1 cut(s) 437
TaqI TCGA 4 cut(s) 180, 312, 398, 623
TasI AATT 1 cut(s) 612
TatI WGTACW 2 cut(s) 9, 297
TauI GCSGC 3 cut(s) 34, 236, 267
TfiI GAWTC 1 cut(s) 217
Tru1I TTAA 3 cut(s) 210, 414, 555
Tru9I TTAA 3 cut(s) 210, 414, 555
TscAI CASTG 1 cut(s) 349
TseI GCWGC 3 cut(s) 34, 267, 370
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 398
XspI CTAG 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.