MD06G1203900.v1.1

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0008993
Physical Location & Seq
Forward (+)
33853359 .. 33855820
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1203900.v1.1.491

Sequence Viewer

Length: 1089 bp
ATGGCGCAGTACAGCAAGGTCGCCCCGACCCCTCTTCTGAAAGACGAGCTCGACATCGTCATCCCCACCATCCGAAACCTCGATTTCCTCGAGATGTGGAGGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCGTCGAAGGTGATCAAGGTCCCGGAGGGGTTCGACTACGAGCTCTACAACCGCAATGACATCAACCGGATTCTGGGTCCCAAGGCGTCCTGCATCTCCTTCAAGGACTCTGCTTGCAGATGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTTGTTGCCAAAGATCCTTCTGGCAAAGATATCAATGCACTTGAGCAGCATATCAAGAACCTTCTGTCTCCGTCAACTCCATTTTTCTTCAACACCCTGTACGACCCATACCGTGAAGGTGCAGATTTCGTTCGTGGATACCCCTTCAGTCTACGTGAAGGCGTCCCAACCGCTGTTTCTCATGGCCTTTGGCTCAACATTCCGGACTATGATGCTCCCACCCAGCTTGTTAAGCCCCGAGAGAGAAACACCAGGTTTGTGGATGCAGTCCTGACCATTCCCAAGGGAACTCTTTTCCCCATGTGCGGCATGAATCTGGCATTCGACCGTGAGTTGATTGGACCTGCTATGTACTTTGGACTCATGGGTGATGGTCAGCCAATCGGGCGCTACGACGACATGTGGGCTGGCTGGTGCATGAAGGTTATTTGTGACCACTTGGGGTTGGGAGTGAAGACAGGATTGCCCTACATCTGGCATAGCAAGGCAAGCAACCCATTTGTGAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAAGAGGAGTTGATCCCGTTTTTCCAGTCCGCCACCCTCCCAAAGGATTGCACCACCGTTCAGGGCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCCAAACTTGGCAAGGTCGATGAGTACTTCGTCAAGCTTGCAGATGCCATGGTCACATGGGTCGAAGCATGGGATGAGTTGAACCCAGTCGAAGGAAAGGCTGTTGAGGCTAACAAAGTTGTTGCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

363

Amino Acids

41.24

Weight (kDa)

6.03

Isoelectric Point (pI)

30.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 13 - 347 3.5e-180 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 676
AccI GTMKAC 1 cut(s) 475
AccIII TCCGGA 1 cut(s) 526
AciI CCGC 4 cut(s) 199, 495, 630, 891
AclWI GGATC 2 cut(s) 332, 868
AcuI CTGAAG 1 cut(s) 454
AcyI GRCGYC 2 cut(s) 233, 486
AfaI GTAC 6 cut(s) 11, 119, 299, 425, 677, 983
AfiI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
AflII CTTAAG 1 cut(s) 833
AflIII ACRYGT 1 cut(s) 723
AgsI TTSAA 4 cut(s) 250, 415, 938, 1039
AjnI CCWGG 2 cut(s) 135, 575
AjuI GAANNNNNNNTTGG 2 cut(s) 484, 516
AluBI AGCT 4 cut(s) 49, 190, 550, 994
AluI AGCT 4 cut(s) 49, 190, 550, 994
Alw21I GWGCWC 2 cut(s) 51, 192
Alw26I GTCTC 2 cut(s) 289, 396
AlwI GGATC 2 cut(s) 332, 868
Ama87I CYCGRG 2 cut(s) 89, 561
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 3 cut(s) 101, 508, 957
ApeKI GCWGC 2 cut(s) 370, 927
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
AspLEI GCGC 2 cut(s) 7, 714
AspS9I GGNCC 4 cut(s) 102, 166, 224, 665
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 3 cut(s) 169, 298, 704
AvaI CYCGRG 2 cut(s) 89, 561
AvaII GGWCC 3 cut(s) 166, 224, 665
BanII GRGCYC 2 cut(s) 51, 192
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 2 cut(s) 51, 192
BbvI GCAGC 2 cut(s) 382, 914
BccI CCATC 3 cut(s) 77, 317, 689
BceAI ACGGC 1 cut(s) 157
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 2 cut(s) 137, 577
BciVI GTATCC 1 cut(s) 455
BclI TGATCA 1 cut(s) 159
BcnI CCSGG 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 289, 396
BfoI RGCGCY 1 cut(s) 715
BfrI CTTAAG 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 676
BfuI GTATCC 1 cut(s) 455
BglI GCCNNNNNGGC 1 cut(s) 709
BisI GCNGC 3 cut(s) 371, 631, 928
BlsI GCNGC 3 cut(s) 372, 632, 929
BmcAI AGTACT 1 cut(s) 983
Bme1390I CCNGG 3 cut(s) 137, 170, 577
Bme18I GGWCC 3 cut(s) 166, 224, 665
BmeT110I CYCGRG 2 cut(s) 89, 561
BmgT120I GGNCC 4 cut(s) 102, 166, 224, 665
BmiI GGNNCC 3 cut(s) 168, 225, 226
BmrFI CCNGG 3 cut(s) 137, 170, 577
BmrI ACTGGG 1 cut(s) 1037
BmsI GCATC 5 cut(s) 249, 257, 526, 577, 991
BmuI ACTGGG 1 cut(s) 1037
BpiI GAAGAC 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 170
BsaAI YACGTR 1 cut(s) 479
BsaHI GRCGYC 2 cut(s) 233, 486
BsaI GGTCTC 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 228, 606, 1005
BsaWI WCCGGW 2 cut(s) 213, 526
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
Bse1I ACTGG 3 cut(s) 863, 886, 1043
Bse3DI GCAATG 1 cut(s) 208
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 2 cut(s) 137, 577
BseDI CCNNGG 3 cut(s) 228, 606, 1005
BseGI GGATG 4 cut(s) 60, 69, 592, 1036
BseLI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
BseMI GCAATG 1 cut(s) 208
BseNI ACTGG 3 cut(s) 863, 886, 1043
BseRI GAGGAG 1 cut(s) 881
BseXI GCAGC 2 cut(s) 382, 914
BseYI CCCAGC 1 cut(s) 546
BsgI GTGCAG 1 cut(s) 465
Bsh1285I CGRYCG 1 cut(s) 652
BshFI GGCC 3 cut(s) 103, 510, 959
BsiEI CGRYCG 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 51, 192
BsiHKCI CYCGRG 2 cut(s) 89, 561
BsiSI CCGG 3 cut(s) 170, 214, 527
BslFI GGGAC 3 cut(s) 152, 210, 473
BslI CCNNNNNNNGG 7 cut(s) 154, 220, 629, 798, 838, 904, 1049
BsmAI GTCTC 2 cut(s) 289, 396
BsmFI GGGAC 3 cut(s) 152, 210, 473
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 3 cut(s) 103, 510, 959
Bso31I GGTCTC 1 cut(s) 289
BsoBI CYCGRG 2 cut(s) 89, 561
Bsp1286I GDGCHC 2 cut(s) 51, 192
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 3 cut(s) 159, 337, 873
Bsp19I CCATGG 1 cut(s) 1005
BspACI CCGC 4 cut(s) 199, 495, 630, 891
BspANI GGCC 3 cut(s) 103, 510, 959
BspEI TCCGGA 1 cut(s) 526
BspLI GGNNCC 3 cut(s) 168, 225, 226
BspMI ACCTGC 1 cut(s) 676
BspPI GGATC 2 cut(s) 332, 868
BspTI CTTAAG 1 cut(s) 833
BspTNI GGTCTC 1 cut(s) 289
BsrDI GCAATG 1 cut(s) 208
BsrI ACTGG 3 cut(s) 863, 886, 1043
BssECI CCNNGG 3 cut(s) 228, 606, 1005
BssMI GATC 3 cut(s) 159, 337, 873
BssNI GRCGYC 2 cut(s) 233, 486
BssT1I CCWWGG 3 cut(s) 228, 606, 1005
Bst2UI CCWGG 2 cut(s) 137, 577
Bst4CI ACNGT 3 cut(s) 437, 653, 919
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 2 cut(s) 233, 486
BstAFI CTTAAG 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 4 cut(s) 262, 733, 814, 996
BstDSI CCRYGG 1 cut(s) 1005
BstENI CCTNNNNNAGG 2 cut(s) 836, 902
BstF5I GGATG 4 cut(s) 60, 69, 592, 1036
BstH2I RGCGCY 1 cut(s) 715
BstHHI GCGC 2 cut(s) 7, 714
BstKTI GATC 3 cut(s) 162, 340, 876
BstMAI GTCTC 2 cut(s) 289, 396
BstMBI GATC 3 cut(s) 159, 337, 873
BstMCI CGRYCG 1 cut(s) 652
BstMWI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
BstNI CCWGG 2 cut(s) 137, 577
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 3 cut(s) 135, 168, 575
BstV1I GCAGC 2 cut(s) 382, 914
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 337
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 337
BsuI GTATCC 1 cut(s) 455
BsuRI GGCC 3 cut(s) 103, 510, 959
BtgI CCRYGG 1 cut(s) 1005
BtsCI GGATG 4 cut(s) 60, 69, 592, 1036
BveI ACCTGC 1 cut(s) 676
Cac8I GCNNGC 4 cut(s) 262, 733, 814, 996
CfoI GCGC 2 cut(s) 7, 714
Cfr13I GGNCC 4 cut(s) 102, 166, 224, 665
CseI GACGC 2 cut(s) 222, 475
CsiI ACCWGGT 1 cut(s) 575
Csp6I GTAC 6 cut(s) 10, 118, 298, 424, 676, 982
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviQI GTAC 6 cut(s) 10, 118, 298, 424, 676, 982
DpnI GATC 3 cut(s) 161, 339, 875
DpnII GATC 3 cut(s) 159, 337, 873
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 880
Ecl136II GAGCTC 2 cut(s) 49, 190
Eco130I CCWWGG 3 cut(s) 228, 606, 1005
Eco24I GRGCYC 2 cut(s) 51, 192
Eco31I GGTCTC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 166, 224, 665
Eco53kI GAGCTC 2 cut(s) 49, 190
Eco57I CTGAAG 1 cut(s) 454
Eco88I CYCGRG 2 cut(s) 89, 561
EcoICRI GAGCTC 2 cut(s) 49, 190
EcoNI CCTNNNNNAGG 2 cut(s) 836, 902
EcoO109I RGGNCCY 3 cut(s) 102, 166, 224
EcoRII CCWGG 2 cut(s) 135, 575
EcoRV GATATC 1 cut(s) 355
EcoT14I CCWWGG 3 cut(s) 228, 606, 1005
EcoT38I GRGCYC 2 cut(s) 51, 192
ErhI CCWWGG 3 cut(s) 228, 606, 1005
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 3 cut(s) 152, 210, 473
FbaI TGATCA 1 cut(s) 159
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 3 cut(s) 371, 631, 928
FokI GGATG 4 cut(s) 47, 56, 599, 1043
FriOI GRGCYC 2 cut(s) 51, 192
Fsp4HI GCNGC 3 cut(s) 371, 631, 928
GlaI GCGC 2 cut(s) 6, 713
GluI GCNGC 3 cut(s) 371, 631, 928
GsaI CCCAGC 1 cut(s) 550
HaeII RGCGCY 1 cut(s) 715
HaeIII GGCC 3 cut(s) 103, 510, 959
HapII CCGG 3 cut(s) 170, 214, 527
HgaI GACGC 2 cut(s) 222, 475
HhaI GCGC 2 cut(s) 7, 714
Hin1I GRCGYC 2 cut(s) 233, 486
Hin6I GCGC 2 cut(s) 5, 712
HinP1I GCGC 2 cut(s) 5, 712
HincII GTYRAC 1 cut(s) 399
HindII GTYRAC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 5 cut(s) 217, 254, 637, 684, 852
HpaII CCGG 3 cut(s) 170, 214, 527
HphI GGTGA 3 cut(s) 169, 298, 704
Hpy166II GTNNAC 3 cut(s) 399, 476, 829
Hpy188I TCNGA 2 cut(s) 39, 74
Hpy188III TCNNGA 4 cut(s) 91, 379, 527, 595
Hpy8I GTNNAC 3 cut(s) 399, 476, 829
Hpy99I CGWCG 4 cut(s) 154, 317, 320, 722
HpyCH4III ACNGT 3 cut(s) 437, 653, 919
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 8 cut(s) 240, 264, 362, 446, 590, 741, 912, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
HpySE526I ACGT 1 cut(s) 478
Hsp92I GRCGYC 2 cut(s) 233, 486
HspAI GCGC 2 cut(s) 5, 712
KflI GGGWCCC 1 cut(s) 224
Kpn2I TCCGGA 1 cut(s) 526
Ksp22I TGATCA 1 cut(s) 159
Kzo9I GATC 3 cut(s) 159, 337, 873
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 2 cut(s) 382, 914
LweI GCATC 5 cut(s) 249, 257, 526, 577, 991
MabI ACCWGGT 1 cut(s) 575
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 755, 1009
MalI GATC 3 cut(s) 161, 339, 875
MboI GATC 3 cut(s) 159, 337, 873
MboII GAAGA 6 cut(s) 26, 100, 295, 304, 403, 790
MflI RGATCY 1 cut(s) 337
MhlI GDGCHC 2 cut(s) 51, 192
MlyI GAGTC 2 cut(s) 248, 678
MnlI CCTC 9 cut(s) 42, 89, 93, 98, 134, 166, 859, 908, 1057
MroI TCCGGA 1 cut(s) 526
MseI TTAA 2 cut(s) 555, 834
MspA1I CMGCKG 1 cut(s) 497
MspCI CTTAAG 1 cut(s) 833
MspI CCGG 3 cut(s) 170, 214, 527
MspR9I CCNGG 3 cut(s) 137, 170, 577
Mva1269I GAATGC 1 cut(s) 644
MvaI CCWGG 2 cut(s) 137, 577
MwoI GCNNNNNNNGC 4 cut(s) 556, 709, 813, 1064
NciI CCSGG 1 cut(s) 170
NcoI CCATGG 1 cut(s) 1005
NdeII GATC 3 cut(s) 159, 337, 873
NlaIV GGNNCC 3 cut(s) 168, 225, 226
NmuCI GTSAC 2 cut(s) 755, 1009
NspI RCATGY 1 cut(s) 727
PaeR7I CTCGAG 1 cut(s) 89
PciI ACATGT 1 cut(s) 723
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 714
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 3 cut(s) 217, 637, 852
PflFI GACNNNGTC 1 cut(s) 56
PfoI TCCNGGA 2 cut(s) 135, 168
PkrI GCNGC 3 cut(s) 372, 632, 929
PleI GAGTC 2 cut(s) 248, 678
PpsI GAGTC 2 cut(s) 248, 678
Ppu21I YACGTR 1 cut(s) 479
PpuMI RGGWCCY 2 cut(s) 166, 224
PscI ACATGT 1 cut(s) 723
Psp124BI GAGCTC 2 cut(s) 51, 192
Psp5II RGGWCCY 2 cut(s) 166, 224
Psp6I CCWGG 2 cut(s) 135, 575
PspFI CCCAGC 1 cut(s) 546
PspGI CCWGG 2 cut(s) 135, 575
PspN4I GGNNCC 3 cut(s) 168, 225, 226
PspPI GGNCC 4 cut(s) 102, 166, 224, 665
PspPPI RGGWCCY 2 cut(s) 166, 224
PsuI RGATCY 1 cut(s) 337
PsyI GACNNNGTC 1 cut(s) 56
RsaI GTAC 6 cut(s) 11, 119, 299, 425, 677, 983
RsaNI GTAC 6 cut(s) 10, 118, 298, 424, 676, 982
SacI GAGCTC 2 cut(s) 51, 192
SaqAI TTAA 2 cut(s) 555, 834
SatI GCNGC 3 cut(s) 371, 631, 928
Sau3AI GATC 3 cut(s) 159, 337, 873
Sau96I GGNCC 4 cut(s) 102, 166, 224, 665
ScaI AGTACT 1 cut(s) 983
SchI GAGTC 2 cut(s) 248, 678
ScrFI CCNGG 3 cut(s) 137, 170, 577
SduI GDGCHC 2 cut(s) 51, 192
SexAI ACCWGGT 1 cut(s) 575
SfaNI GCATC 5 cut(s) 249, 257, 526, 577, 991
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 3 cut(s) 166, 224, 665
SlaI CTCGAG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 89, 365, 833
SmoI CTYRAG 3 cut(s) 89, 365, 833
SsiI CCGC 4 cut(s) 199, 495, 630, 891
SstI GAGCTC 2 cut(s) 51, 192
StyD4I CCNGG 3 cut(s) 135, 168, 575
StyI CCWWGG 3 cut(s) 228, 606, 1005
TaaI ACNGT 3 cut(s) 437, 653, 919
TaiI ACGT 1 cut(s) 481
TatI WGTACW 4 cut(s) 9, 297, 675, 981
TauI GCSGC 1 cut(s) 633
TfiI GAWTC 3 cut(s) 217, 637, 852
Tru1I TTAA 2 cut(s) 555, 834
Tru9I TTAA 2 cut(s) 555, 834
TseFI GTSAC 2 cut(s) 755, 1009
TseI GCWGC 2 cut(s) 370, 927
Tsp45I GTSAC 2 cut(s) 755, 1009
TspDTI ATGAA 2 cut(s) 650, 758
TspGWI ACGGA 1 cut(s) 384
Tth111I GACNNNGTC 1 cut(s) 56
Vha464I CTTAAG 1 cut(s) 833
VpaK11BI GGWCC 3 cut(s) 166, 224, 665
XagI CCTNNNNNAGG 2 cut(s) 836, 902
XceI RCATGY 1 cut(s) 727
XhoI CTCGAG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 475
ZrmI AGTACT 1 cut(s) 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.