RchiOBHm_Chr7g0183481

Alpha-1,4-glucan-protein synthase UDP-forming

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008993
Physical Location & Seq
Forward (+)
4341866 .. 4343972
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16370

Sequence Viewer

Length: 1050 bp
ATGGCGGAGTACAGCAAGGTCGCGCCGACGCCGCTGCTGAAGGATGAGCTGGACATAGCCCTTCTTCCAGCAGTACCACCTCATCATCGTCCAGGACGGCGACCCCAGCAAGGTCATCAAGGTCCCGAGGGCTTCGACTACGAGCTTTACAACCGCAACGACATTAACCGGATTCTCGGCCCCAAAGCCGCCTGTATCTCCTTCAAGGACTCCGCCTGCCGCTGCTTCGGCTACATGGTCTCCAAGAAGAAGTACATCTTCACCATCGACGACGATTGCTTCGTTGCGAAAGATCCCACTGGAAAGGAGATCAATGCGCTAGAGCAGCACATCAAGAACCTTCTGTGCCCGTCGACTCCATTTTTCTTTAACACCCTGTACGACCCATACCGTGAAGGTGCAGATTTTGTCCGCGGTTACCCTTTCAGTCTGCGTGAAGGTGTGCCAACCGCTGTTTCTCACGGCCTCTGGCTCAACATTCCTGATTATGATGCACCTACCCAGCTTGTTAAGCCTCTTGAGAGGAACACCAGGTTTGTGGATGCAGTCTTGACCATCCCCAAGGGAACTCTCTTTCCCATGTGTGGGATGAATCTGGCATTCAACCGTGAGTTGATCGGCCCTGCCATGTACTTTGGACTCATGGGCGATGGGCAGCCAATTGGGCGCTACGACGATATGTGGGCTGGATGGTGCATGAAGGTTATTTGTGACCACTTGGGGCTAGGAGTGAAGACGGGATTGCCCTACATCTGGCATAGCAAAGCCAGCAACCCATTTGTTAACCTTAAGAAGGAATACAAAGGAATCTACTGGCAGGAAGAGTTGATCCCATTTTTCCAATCTGCTACGCTTCCCAAGGATTGCACCACTGTTCAGGCCTGCTACCTTGAACTCTCCAAGCAAGTCAAGGCGAAACTTGGTAAGGTTGATGACTACTTCAACAAGCTTGCAGATGCCATGGTTACATGGATCGAAGCATGGGATGAGTTGAACCCGGCTGGGGGGAAAGCTGTTGAGCTCAACGGACCTGCAAAATCTTCGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

349

Amino Acids

39.28

Weight (kDa)

7.02

Isoelectric Point (pI)

38.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 40 - 332 2e-158 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1039
AccI GTMKAC 1 cut(s) 353
AccII CGCG 2 cut(s) 23, 414
AciI CCGC 9 cut(s) 5, 32, 154, 189, 213, 220, 412, 414, 450
AclWI GGATC 3 cut(s) 287, 823, 980
AcuI CTGAAG 1 cut(s) 59
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 5 cut(s) 11, 75, 254, 380, 632
AfiI CCNNNNNNNGG 7 cut(s) 110, 584, 585, 753, 793, 1003, 1004
AflII CTTAAG 1 cut(s) 788
AgsI TTSAA 5 cut(s) 205, 604, 893, 943, 994
AjnI CCWGG 2 cut(s) 91, 530
AjuI GAANNNNNNNTTGG 2 cut(s) 439, 471
AloI GAACNNNNNNTCC 2 cut(s) 559, 591
AluBI AGCT 6 cut(s) 49, 145, 505, 949, 1013, 1021
AluI AGCT 6 cut(s) 49, 145, 505, 949, 1013, 1021
Alw21I GWGCWC 1 cut(s) 1023
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 3 cut(s) 287, 823, 980
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 4 cut(s) 178, 463, 619, 879
ApeKI GCWGC 4 cut(s) 34, 222, 325, 655
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspLEI GCGC 3 cut(s) 25, 319, 669
AspS9I GGNCC 4 cut(s) 122, 179, 620, 1028
AsuC2I CCSGG 1 cut(s) 998
AsuHPI GGTGA 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 1043
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 2 cut(s) 122, 1028
BaeGI GKGCMC 1 cut(s) 350
BanII GRGCYC 1 cut(s) 1023
BbsI GAAGAC 1 cut(s) 740
Bbv12I GWGCWC 1 cut(s) 1023
BbvI GCAGC 4 cut(s) 21, 209, 337, 667
BccI CCATC 4 cut(s) 272, 563, 644, 684
BceAI ACGGC 2 cut(s) 113, 478
BcgI CGANNNNNNTGC 3 cut(s) 16, 50, 1023
BciT130I CCWGG 2 cut(s) 93, 532
BcnI CCSGG 1 cut(s) 998
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 2 cut(s) 320, 725
BfoI RGCGCY 1 cut(s) 670
BfrI CTTAAG 1 cut(s) 788
BfuAI ACCTGC 1 cut(s) 1039
BglI GCCNNNNNGGC 1 cut(s) 664
BisI GCNGC 7 cut(s) 32, 35, 189, 220, 223, 326, 656
BlsI GCNGC 7 cut(s) 33, 36, 190, 221, 224, 327, 657
Bme1390I CCNGG 3 cut(s) 93, 532, 998
Bme18I GGWCC 2 cut(s) 122, 1028
BmeT110I CYCGRG 1 cut(s) 125
BmgT120I GGNCC 4 cut(s) 122, 179, 620, 1028
BmiI GGNNCC 2 cut(s) 124, 181
BmrFI CCNGG 3 cut(s) 93, 532, 998
BmsI GCATC 3 cut(s) 481, 532, 946
BpiI GAAGAC 1 cut(s) 740
Bpu14I TTCGAA 1 cut(s) 1043
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 998
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 5 cut(s) 126, 412, 561, 858, 960
BsaWI WCCGGW 1 cut(s) 168
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 7 cut(s) 110, 584, 585, 753, 793, 1003, 1004
Bse1I ACTGG 2 cut(s) 304, 818
BseBI CCWGG 2 cut(s) 93, 532
BseDI CCNNGG 5 cut(s) 126, 412, 561, 858, 960
BseGI GGATG 6 cut(s) 49, 547, 555, 594, 695, 991
BseLI CCNNNNNNNGG 7 cut(s) 110, 584, 585, 753, 793, 1003, 1004
BseNI ACTGG 2 cut(s) 304, 818
BseSI GKGCMC 1 cut(s) 350
BseXI GCAGC 4 cut(s) 21, 209, 337, 667
BseYI CCCAGC 3 cut(s) 105, 501, 1001
BsgI GTGCAG 1 cut(s) 420
Bsh1236I CGCG 2 cut(s) 23, 414
BshFI GGCC 4 cut(s) 180, 465, 621, 881
BsiHKAI GWGCWC 1 cut(s) 1023
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 2 cut(s) 169, 998
BslFI GGGAC 1 cut(s) 108
BslI CCNNNNNNNGG 7 cut(s) 110, 584, 585, 753, 793, 1003, 1004
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 108
BsmI GAATGC 1 cut(s) 599
BsnI GGCC 4 cut(s) 180, 465, 621, 881
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 125
Bsp119I TTCGAA 1 cut(s) 1043
Bsp1286I GDGCHC 2 cut(s) 350, 1023
Bsp143I GATC 5 cut(s) 292, 309, 615, 828, 972
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 9 cut(s) 5, 32, 154, 189, 213, 220, 412, 414, 450
BspANI GGCC 4 cut(s) 180, 465, 621, 881
BspFNI CGCG 2 cut(s) 23, 414
BspLI GGNNCC 2 cut(s) 124, 181
BspMI ACCTGC 1 cut(s) 1039
BspPI GGATC 3 cut(s) 287, 823, 980
BspT104I TTCGAA 1 cut(s) 1043
BspTI CTTAAG 1 cut(s) 788
BspTNI GGTCTC 1 cut(s) 244
BsrI ACTGG 2 cut(s) 304, 818
BssECI CCNNGG 5 cut(s) 126, 412, 561, 858, 960
BssMI GATC 5 cut(s) 292, 309, 615, 828, 972
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 3 cut(s) 561, 858, 960
Bst2UI CCWGG 2 cut(s) 93, 532
Bst4CI ACNGT 3 cut(s) 392, 608, 874
Bst6I CTCTTC 1 cut(s) 816
BstACI GRCGYC 1 cut(s) 29
BstAFI CTTAAG 1 cut(s) 788
BstBI TTCGAA 1 cut(s) 1043
BstC8I GCNNGC 4 cut(s) 217, 769, 883, 951
BstDSI CCRYGG 2 cut(s) 412, 960
BstEII GGTNACC 1 cut(s) 416
BstENI CCTNNNNNAGG 1 cut(s) 791
BstF5I GGATG 6 cut(s) 49, 547, 555, 594, 695, 991
BstFNI CGCG 2 cut(s) 23, 414
BstH2I RGCGCY 1 cut(s) 670
BstHHI GCGC 3 cut(s) 25, 319, 669
BstKTI GATC 5 cut(s) 295, 312, 618, 831, 975
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 5 cut(s) 292, 309, 615, 828, 972
BstMWI GCNNNNNNNGC 7 cut(s) 31, 106, 228, 325, 511, 664, 768
BstNI CCWGG 2 cut(s) 93, 532
BstPI GGTNACC 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 91, 530, 996
BstSLI GKGCMC 1 cut(s) 350
BstUI CGCG 2 cut(s) 23, 414
BstV1I GCAGC 4 cut(s) 21, 209, 337, 667
BstV2I GAAGAC 1 cut(s) 740
BstX2I RGATCY 1 cut(s) 292
BstXI CCANNNNNNTGG 1 cut(s) 538
BstYI RGATCY 1 cut(s) 292
BsuRI GGCC 4 cut(s) 180, 465, 621, 881
BtgI CCRYGG 2 cut(s) 412, 960
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 6 cut(s) 49, 547, 555, 594, 695, 991
BtsIMutI CAGTG 2 cut(s) 297, 870
BveI ACCTGC 1 cut(s) 1039
Cac8I GCNNGC 4 cut(s) 217, 769, 883, 951
CfoI GCGC 3 cut(s) 25, 319, 669
Cfr13I GGNCC 4 cut(s) 122, 179, 620, 1028
Cfr42I CCGCGG 1 cut(s) 415
CseI GACGC 1 cut(s) 37
CsiI ACCWGGT 1 cut(s) 530
Csp6I GTAC 5 cut(s) 10, 74, 253, 379, 631
CviAII CATG 8 cut(s) 235, 580, 628, 643, 697, 961, 969, 981
CviQI GTAC 5 cut(s) 10, 74, 253, 379, 631
DpnI GATC 5 cut(s) 294, 311, 617, 830, 974
DpnII GATC 5 cut(s) 292, 309, 615, 828, 972
Eam1104I CTCTTC 1 cut(s) 816
EarI CTCTTC 1 cut(s) 816
EciI GGCGGA 2 cut(s) 20, 202
Ecl136II GAGCTC 1 cut(s) 1021
Eco130I CCWWGG 3 cut(s) 561, 858, 960
Eco147I AGGCCT 1 cut(s) 881
Eco24I GRGCYC 1 cut(s) 1023
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 122, 1028
Eco53kI GAGCTC 1 cut(s) 1021
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 125
Eco91I GGTNACC 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 1021
EcoNI CCTNNNNNAGG 1 cut(s) 791
EcoO109I RGGNCCY 1 cut(s) 122
EcoO65I GGTNACC 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 91, 530
EcoT14I CCWWGG 3 cut(s) 561, 858, 960
EcoT38I GRGCYC 1 cut(s) 1023
ErhI CCWWGG 3 cut(s) 561, 858, 960
FaeI CATG 8 cut(s) 238, 583, 631, 646, 700, 964, 972, 984
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FaqI GGGAC 1 cut(s) 108
FatI CATG 8 cut(s) 234, 579, 627, 642, 696, 960, 968, 980
FblI GTMKAC 1 cut(s) 353
Fnu4HI GCNGC 7 cut(s) 32, 35, 189, 220, 223, 326, 656
FokI GGATG 6 cut(s) 56, 542, 554, 601, 702, 998
FriOI GRGCYC 1 cut(s) 1023
Fsp4HI GCNGC 7 cut(s) 32, 35, 189, 220, 223, 326, 656
FspBI CTAG 2 cut(s) 320, 725
GlaI GCGC 3 cut(s) 24, 318, 668
GluI GCNGC 7 cut(s) 32, 35, 189, 220, 223, 326, 656
GsaI CCCAGC 3 cut(s) 109, 505, 1005
HaeII RGCGCY 1 cut(s) 670
HaeIII GGCC 4 cut(s) 180, 465, 621, 881
HapII CCGG 2 cut(s) 169, 998
HgaI GACGC 1 cut(s) 37
HhaI GCGC 3 cut(s) 25, 319, 669
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 8 cut(s) 238, 583, 631, 646, 700, 964, 972, 984
Hin6I GCGC 3 cut(s) 23, 317, 667
HinP1I GCGC 3 cut(s) 23, 317, 667
HincII GTYRAC 2 cut(s) 354, 784
HindII GTYRAC 2 cut(s) 354, 784
HindIII AAGCTT 1 cut(s) 947
HinfI GANTC 6 cut(s) 172, 209, 355, 592, 639, 807
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 2 cut(s) 169, 998
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 2 cut(s) 354, 784
Hpy188III TCNNGA 5 cut(s) 125, 334, 482, 518, 550
Hpy8I GTNNAC 2 cut(s) 354, 784
Hpy99I CGWCG 5 cut(s) 31, 272, 275, 355, 677
HpyAV CCTTC 8 cut(s) 34, 71, 211, 350, 389, 431, 694, 787
HpyCH4III ACNGT 3 cut(s) 392, 608, 874
HpyCH4V TGCA 7 cut(s) 401, 494, 545, 696, 867, 953, 1034
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 106, 228, 325, 511, 664, 768
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 8 cut(s) 238, 583, 631, 646, 700, 964, 972, 984
HspAI GCGC 3 cut(s) 23, 317, 667
KspAI GTTAAC 1 cut(s) 784
KspI CCGCGG 1 cut(s) 415
Kzo9I GATC 5 cut(s) 292, 309, 615, 828, 972
Lsp1109I GCAGC 4 cut(s) 21, 209, 337, 667
LweI GCATC 3 cut(s) 481, 532, 946
MabI ACCWGGT 1 cut(s) 530
MaeI CTAG 2 cut(s) 320, 725
MaeIII GTNAC 3 cut(s) 416, 710, 964
MalI GATC 5 cut(s) 294, 311, 617, 830, 974
MboI GATC 5 cut(s) 292, 309, 615, 828, 972
MboII GAAGA 6 cut(s) 56, 250, 259, 745, 833, 1032
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 1 cut(s) 292
MhlI GDGCHC 2 cut(s) 350, 1023
MluCI AATT 1 cut(s) 660
MlyI GAGTC 3 cut(s) 203, 349, 633
MnlI CCTC 5 cut(s) 90, 121, 476, 516, 525
MseI TTAA 5 cut(s) 165, 369, 510, 783, 789
MspA1I CMGCKG 4 cut(s) 34, 222, 414, 452
MspCI CTTAAG 1 cut(s) 788
MspI CCGG 2 cut(s) 169, 998
MspR9I CCNGG 3 cut(s) 93, 532, 998
MunI CAATTG 1 cut(s) 660
Mva1269I GAATGC 1 cut(s) 599
MvaI CCWGG 2 cut(s) 93, 532
MvnI CGCG 2 cut(s) 23, 414
MwoI GCNNNNNNNGC 7 cut(s) 31, 106, 228, 325, 511, 664, 768
NciI CCSGG 1 cut(s) 998
NcoI CCATGG 1 cut(s) 960
NdeII GATC 5 cut(s) 292, 309, 615, 828, 972
NlaIII CATG 8 cut(s) 238, 583, 631, 646, 700, 964, 972, 984
NlaIV GGNNCC 2 cut(s) 124, 181
NmeAIII GCCGAG 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 710
NspV TTCGAA 1 cut(s) 1043
PceI AGGCCT 1 cut(s) 881
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PctI GAATGC 1 cut(s) 599
PfeI GAWTC 3 cut(s) 172, 592, 807
PfoI TCCNGGA 1 cut(s) 91
PkrI GCNGC 7 cut(s) 33, 36, 190, 221, 224, 327, 657
PleI GAGTC 3 cut(s) 203, 349, 633
PpsI GAGTC 3 cut(s) 203, 349, 633
PpuMI RGGWCCY 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 1023
Psp5II RGGWCCY 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 91, 530
PspEI GGTNACC 1 cut(s) 416
PspFI CCCAGC 3 cut(s) 105, 501, 1001
PspGI CCWGG 2 cut(s) 91, 530
PspN4I GGNNCC 2 cut(s) 124, 181
PspPI GGNCC 4 cut(s) 122, 179, 620, 1028
PspPPI RGGWCCY 1 cut(s) 122
PsuI RGATCY 1 cut(s) 292
RsaI GTAC 5 cut(s) 11, 75, 254, 380, 632
RsaNI GTAC 5 cut(s) 10, 74, 253, 379, 631
SacI GAGCTC 1 cut(s) 1023
SacII CCGCGG 1 cut(s) 415
SalI GTCGAC 1 cut(s) 352
SaqAI TTAA 5 cut(s) 165, 369, 510, 783, 789
SatI GCNGC 7 cut(s) 32, 35, 189, 220, 223, 326, 656
Sau3AI GATC 5 cut(s) 292, 309, 615, 828, 972
Sau96I GGNCC 4 cut(s) 122, 179, 620, 1028
SchI GAGTC 3 cut(s) 203, 349, 633
ScrFI CCNGG 3 cut(s) 93, 532, 998
SduI GDGCHC 2 cut(s) 350, 1023
SexAI ACCWGGT 1 cut(s) 530
SfaNI GCATC 3 cut(s) 481, 532, 946
Sfr303I CCGCGG 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 1043
SgrBI CCGCGG 1 cut(s) 415
SinI GGWCC 2 cut(s) 122, 1028
SmlI CTYRAG 2 cut(s) 518, 788
SmoI CTYRAG 2 cut(s) 518, 788
Sse9I AATT 1 cut(s) 660
SseBI AGGCCT 1 cut(s) 881
SsiI CCGC 9 cut(s) 5, 32, 154, 189, 213, 220, 412, 414, 450
SspMI CTAG 2 cut(s) 320, 725
SstI GAGCTC 1 cut(s) 1023
StuI AGGCCT 1 cut(s) 881
StyD4I CCNGG 3 cut(s) 91, 530, 996
StyI CCWWGG 3 cut(s) 561, 858, 960
TaaI ACNGT 3 cut(s) 392, 608, 874
TaqI TCGA 5 cut(s) 135, 267, 353, 975, 1043
TasI AATT 1 cut(s) 660
TatI WGTACW 3 cut(s) 9, 252, 630
TauI GCSGC 3 cut(s) 34, 191, 222
TfiI GAWTC 3 cut(s) 172, 592, 807
Tru1I TTAA 5 cut(s) 165, 369, 510, 783, 789
Tru9I TTAA 5 cut(s) 165, 369, 510, 783, 789
TscAI CASTG 2 cut(s) 304, 877
TseFI GTSAC 1 cut(s) 710
TseI GCWGC 4 cut(s) 34, 222, 325, 655
Tsp45I GTSAC 1 cut(s) 710
TspDTI ATGAA 2 cut(s) 605, 713
TspGWI ACGGA 1 cut(s) 1041
TspRI CASTG 2 cut(s) 304, 877
Vha464I CTTAAG 1 cut(s) 788
VpaK11BI GGWCC 2 cut(s) 122, 1028
XagI CCTNNNNNAGG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 353
XspI CTAG 2 cut(s) 320, 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.