AT3G09300

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0006513
Physical Location & Seq
Forward (+)
2857847 .. 2860668
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09300.1

Sequence Viewer

Length: 1377 bp
ATGGCTCCTAATGATCCTAAAAAAGCCGTTGGTGGCGGCGGAAGTGGTTTCTTCGCCTCTCTTGCTTCGTCGATCTCCAATTTGGGATCTGCTATGACCAAATCAGTTAATGGTTTGGTTCCCTATGAGGGACTTGAAGTTATCAATCCTGAAGGAAGTACAGATGATGCTGAGGAGGAAGCAAGCAGAGGAAGATGGAAGCAAGAGGATCGAGATGGCTATTGGAAGATGATGCAGAAGTACATAGGATCTGATGTTACATCAATGGTGACCCTTCCTGTGATTATTTTTGAACCAATGACAATGCTTCAGAAAATGGCGGAGTTGATGGAATACTCGCATCTGCTAGACATGGCAGACAAAACCGAGGACCCTTATTTGCGCATGGTGTATGCATCATCGTGGGCTATATCCGTGTATTATGCTTTCCAACGTACCTGGAAACCATTCAATCCAATCCTTGGTGAGACTTATGAGATGGCTAATTACAATGGTGTTAACTTCATATCTGAACAGGTCAGCCATCACCCACCAATGAGTGCTGGTCATGCTGAAAATGAGCATTTTACGTATGATTGTACTTCAAAACTGAAAACAAAATTTTTGGGCAATTCCATTGACGTTTACCCAGTAGGAAGGACACGGGTGACACTTAAAAGAGATGGAGTTGTTCTTGACTTGGTACCTCCTCTGACCAAAGTTCACAACCTAATCTTTGGACGAACTTGGGTCGATTCGCCTGGGGAAATGATCATGACAAATCAGACCACCGGTGACAAAGTCGTGCTTTACTTTCAACCATGTGGCTGGTTCGGATCTGGTCGATATGAAGTGGATGGATATGTCTATAACGCCTCTGAGGAGCCTAAGATACTCATGACTGGCAAATGGAACGAGTCCATGAGTTATCAGCCGTGTGATGGTGAAGGTGAACCTCTCCCAGGCACCGAACTGAAAGAGGTATGGAAACTCGCTGATGTGCCAAAGGATGACAAATATCAATACACTCACTTTGCTCACAAGATTAATAGCTTCGACACTGCCCCGAAAAAGCTGTTGCCCTCTGATTCACGGTTACGACCTGATAGATACGCACTTGAGATGGGCGACATGTCCAAATCAGGCTATGAGAAGAGCAGCATGGAAGAGAGACAGAGAGCTGAAAAGAGAACCCGCGAAGAGAAAGGCCAAGCCTTTACTCCAAAATGGTTCGATGTAACGGAAGAAGTCACTGCTACACCATGGGGTGATCTGGAAGTTTACCAATTCAATGGAAAGTACTCGGAACATCGTGCAGCTGCGGATAACTCTGAAGATAACACCGACCCTAAGTCGATCCAATTCAACCCATGGCAATTCCAAGATCTGTCTACTTAA

Protein Analysis

458

Amino Acids

51.96

Weight (kDa)

5.06

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 74 - 431 1.8e-113 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 383
Acc65I GGTACC 1 cut(s) 682
AccB1I GGYRCC 2 cut(s) 682, 944
AccB7I CCANNNNNTGG 1 cut(s) 461
AccI GTMKAC 1 cut(s) 1370
AccII CGCG 1 cut(s) 1176
AciI CCGC 5 cut(s) 36, 39, 320, 1174, 1301
AclWI GGATC 6 cut(s) 8, 94, 216, 256, 823, 1330
AcsI RAATTY 1 cut(s) 599
AcuI CTGAAG 3 cut(s) 171, 293, 1332
AfaI GTAC 6 cut(s) 160, 242, 436, 580, 684, 1280
AfiI CCNNNNNNNGG 4 cut(s) 128, 461, 920, 941
AflIII ACRYGT 1 cut(s) 1110
AgeI ACCGGT 1 cut(s) 770
AgsI TTSAA 7 cut(s) 137, 293, 451, 585, 797, 1270, 1345
AhdI GACNNNNNGTC 1 cut(s) 1330
AjnI CCWGG 3 cut(s) 437, 739, 940
AluBI AGCT 4 cut(s) 1032, 1054, 1160, 1298
AluI AGCT 4 cut(s) 1032, 1054, 1160, 1298
Alw26I GTCTC 2 cut(s) 461, 1144
AlwI GGATC 6 cut(s) 8, 94, 216, 256, 823, 1330
AoxI GGCC 1 cut(s) 1186
ApeKI GCWGC 3 cut(s) 1137, 1295, 1298
ApoI RAATTY 1 cut(s) 599
AseI ATTAAT 1 cut(s) 1026
AsiGI ACCGGT 1 cut(s) 770
Asp700I GAANNNNTTC 1 cut(s) 446
Asp718I GGTACC 1 cut(s) 682
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 8 cut(s) 280, 476, 518, 658, 785, 935, 941, 1259
AvaII GGWCC 1 cut(s) 370
BanI GGYRCC 2 cut(s) 682, 944
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 3 cut(s) 1149, 1285, 1307
BccI CCATC 9 cut(s) 189, 209, 322, 472, 531, 656, 830, 914, 1096
BceAI ACGGC 2 cut(s) 11, 898
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciT130I CCWGG 3 cut(s) 439, 741, 942
BclI TGATCA 1 cut(s) 750
BcoDI GTCTC 2 cut(s) 461, 1144
BfaI CTAG 1 cut(s) 347
BglII AGATCT 1 cut(s) 1363
BisI GCNGC 4 cut(s) 37, 1138, 1296, 1299
BlsI GCNGC 4 cut(s) 38, 1139, 1297, 1300
BmcAI AGTACT 1 cut(s) 1280
Bme1390I CCNGG 3 cut(s) 439, 741, 942
Bme18I GGWCC 1 cut(s) 370
BmeRI GACNNNNNGTC 1 cut(s) 1330
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 6 cut(s) 6, 120, 372, 684, 864, 946
BmrFI CCNGG 3 cut(s) 439, 741, 942
BmrI ACTGGG 1 cut(s) 623
BmsI GCATC 4 cut(s) 157, 222, 349, 404
BmuI ACTGGG 1 cut(s) 623
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 1118
BsaAI YACGTR 1 cut(s) 570
BsaJI CCNNGG 6 cut(s) 366, 460, 740, 940, 1241, 1349
BsaWI WCCGGW 1 cut(s) 770
Bsc4I CCNNNNNNNGG 4 cut(s) 128, 461, 920, 941
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 629, 886
BseBI CCWGG 3 cut(s) 439, 741, 942
BseDI CCNNGG 6 cut(s) 366, 460, 740, 940, 1241, 1349
BseGI GGATG 2 cut(s) 841, 994
BseLI CCNNNNNNNGG 4 cut(s) 128, 461, 920, 941
BseMII CTCAG 2 cut(s) 162, 849
BseNI ACTGG 2 cut(s) 629, 886
BseRI GAGGAG 3 cut(s) 188, 678, 875
BseXI GCAGC 3 cut(s) 1149, 1285, 1307
BsgI GTGCAG 1 cut(s) 1314
Bsh1236I CGCG 1 cut(s) 1176
BshFI GGCC 1 cut(s) 1188
BshNI GGYRCC 2 cut(s) 682, 944
BshTI ACCGGT 1 cut(s) 770
BsiSI CCGG 1 cut(s) 771
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 4 cut(s) 128, 461, 920, 941
BsmAI GTCTC 2 cut(s) 461, 1144
BsmFI GGGAC 1 cut(s) 144
BsnI GGCC 1 cut(s) 1188
Bsp19I CCATGG 2 cut(s) 1241, 1349
BspACI CCGC 5 cut(s) 36, 39, 320, 1174, 1301
BspANI GGCC 1 cut(s) 1188
BspCNI CTCAG 2 cut(s) 163, 850
BspFNI CGCG 1 cut(s) 1176
BspHI TCATGA 2 cut(s) 753, 876
BspLI GGNNCC 6 cut(s) 6, 120, 372, 684, 864, 946
BspPI GGATC 6 cut(s) 8, 94, 216, 256, 823, 1330
BspQI GCTCTTC 1 cut(s) 1127
BspT107I GGYRCC 2 cut(s) 682, 944
BsrFI RCCGGY 1 cut(s) 770
BsrI ACTGG 2 cut(s) 629, 886
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 6 cut(s) 366, 460, 740, 940, 1241, 1349
BssT1I CCWWGG 3 cut(s) 460, 1241, 1349
Bst2UI CCWGG 3 cut(s) 439, 741, 942
Bst4CI ACNGT 1 cut(s) 1074
Bst6I CTCTTC 3 cut(s) 1127, 1140, 1173
BstBAI YACGTR 1 cut(s) 570
BstC8I GCNNGC 1 cut(s) 184
BstDEI CTNAG 4 cut(s) 171, 858, 867, 1329
BstDSI CCRYGG 2 cut(s) 1241, 1349
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 939
BstF5I GGATG 2 cut(s) 841, 994
BstFNI CGCG 1 cut(s) 1176
BstHHI GCGC 1 cut(s) 384
BstMAI GTCTC 2 cut(s) 461, 1144
BstMWI GCNNNNNNNGC 2 cut(s) 62, 548
BstNI CCWGG 3 cut(s) 439, 741, 942
BstNSI RCATGY 1 cut(s) 1114
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 3 cut(s) 437, 739, 940
BstSNI TACGTA 1 cut(s) 570
BstUI CGCG 1 cut(s) 1176
BstV1I GCAGC 3 cut(s) 1149, 1285, 1307
BstX2I RGATCY 4 cut(s) 86, 248, 815, 1363
BstXI CCANNNNNNTGG 2 cut(s) 807, 1271
BstYI RGATCY 4 cut(s) 86, 248, 815, 1363
BsuRI GGCC 1 cut(s) 1188
BtgI CCRYGG 2 cut(s) 1241, 1349
BtsCI GGATG 2 cut(s) 841, 994
BtsI GCAGTG 2 cut(s) 1038, 1230
BtsIMutI CAGTG 2 cut(s) 1038, 1230
Cac8I GCNNGC 1 cut(s) 184
CciI TCATGA 2 cut(s) 753, 876
CfoI GCGC 1 cut(s) 384
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 1 cut(s) 370
Csp6I GTAC 6 cut(s) 159, 241, 435, 579, 683, 1279
CspAI ACCGGT 1 cut(s) 770
CviQI GTAC 6 cut(s) 159, 241, 435, 579, 683, 1279
DdeI CTNAG 4 cut(s) 171, 858, 867, 1329
DriI GACNNNNNGTC 1 cut(s) 1330
Eam1104I CTCTTC 3 cut(s) 1127, 1140, 1173
Eam1105I GACNNNNNGTC 1 cut(s) 1330
EarI CTCTTC 3 cut(s) 1127, 1140, 1173
EciI GGCGGA 2 cut(s) 54, 335
Eco105I TACGTA 1 cut(s) 570
Eco130I CCWWGG 3 cut(s) 460, 1241, 1349
Eco47I GGWCC 1 cut(s) 370
Eco57I CTGAAG 3 cut(s) 171, 293, 1332
Eco91I GGTNACC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 939
EcoO109I RGGNCCY 1 cut(s) 370
EcoO65I GGTNACC 1 cut(s) 268
EcoRII CCWGG 3 cut(s) 437, 739, 940
EcoT14I CCWWGG 3 cut(s) 460, 1241, 1349
EcoT22I ATGCAT 1 cut(s) 397
ErhI CCWWGG 3 cut(s) 460, 1241, 1349
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 1 cut(s) 144
FauI CCCGC 1 cut(s) 1181
FbaI TGATCA 1 cut(s) 750
FblI GTMKAC 1 cut(s) 1370
Fnu4HI GCNGC 4 cut(s) 37, 1138, 1296, 1299
FokI GGATG 2 cut(s) 848, 1001
Fsp4HI GCNGC 4 cut(s) 37, 1138, 1296, 1299
FspBI CTAG 1 cut(s) 347
FspI TGCGCA 1 cut(s) 383
GlaI GCGC 1 cut(s) 383
GluI GCNGC 4 cut(s) 37, 1138, 1296, 1299
HaeIII GGCC 1 cut(s) 1188
HapII CCGG 1 cut(s) 771
HhaI GCGC 1 cut(s) 384
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 3 cut(s) 734, 896, 1067
HpaI GTTAAC 1 cut(s) 499
HpaII CCGG 1 cut(s) 771
HphI GGTGA 8 cut(s) 280, 476, 518, 658, 785, 935, 941, 1259
Hpy166II GTNNAC 6 cut(s) 499, 625, 703, 932, 1261, 1371
Hpy188III TCNNGA 6 cut(s) 149, 212, 674, 754, 877, 1253
Hpy8I GTNNAC 6 cut(s) 499, 625, 703, 932, 1261, 1371
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 146, 284, 630, 920
HpyCH4III ACNGT 1 cut(s) 1074
HpyCH4IV ACGT 3 cut(s) 433, 569, 621
HpyCH4V TGCA 3 cut(s) 235, 395, 1295
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 548
HpyF3I CTNAG 4 cut(s) 171, 858, 867, 1329
HpySE526I ACGT 3 cut(s) 433, 569, 621
HspAI GCGC 1 cut(s) 382
KpnI GGTACC 1 cut(s) 686
Ksp22I TGATCA 1 cut(s) 750
KspAI GTTAAC 1 cut(s) 499
LguI GCTCTTC 1 cut(s) 1127
LmnI GCTCC 2 cut(s) 10, 862
Lsp1109I GCAGC 3 cut(s) 1149, 1285, 1307
LweI GCATC 4 cut(s) 157, 222, 349, 404
MaeI CTAG 1 cut(s) 347
MaeII ACGT 3 cut(s) 433, 569, 621
MaeIII GTNAC 7 cut(s) 256, 268, 646, 773, 1074, 1216, 1228
MboII GAAGA 8 cut(s) 43, 204, 238, 1144, 1157, 1190, 1235, 1325
MflI RGATCY 4 cut(s) 86, 248, 815, 1363
MluCI AATT 7 cut(s) 79, 484, 599, 610, 1265, 1340, 1355
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 1 cut(s) 454
Mph1103I ATGCAT 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 446
MseI TTAA 5 cut(s) 108, 498, 654, 1026, 1375
MslI CAYNNNNRTG 1 cut(s) 400
MspA1I CMGCKG 1 cut(s) 1298
MspI CCGG 1 cut(s) 771
MspR9I CCNGG 3 cut(s) 439, 741, 942
MvaI CCWGG 3 cut(s) 439, 741, 942
MvnI CGCG 1 cut(s) 1176
MwoI GCNNNNNNNGC 2 cut(s) 62, 548
NcoI CCATGG 2 cut(s) 1241, 1349
NlaIV GGNNCC 6 cut(s) 6, 120, 372, 684, 864, 946
NmuCI GTSAC 4 cut(s) 268, 646, 773, 1228
NsbI TGCGCA 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 397
NspI RCATGY 1 cut(s) 1114
PagI TCATGA 2 cut(s) 753, 876
PciI ACATGT 1 cut(s) 1110
PciSI GCTCTTC 1 cut(s) 1127
PdmI GAANNNNTTC 1 cut(s) 446
PfeI GAWTC 2 cut(s) 734, 1067
PflFI GACNNNGTC 1 cut(s) 779
PflMI CCANNNNNTGG 1 cut(s) 461
PinAI ACCGGT 1 cut(s) 770
PkrI GCNGC 4 cut(s) 38, 1139, 1297, 1300
PleI GAGTC 1 cut(s) 904
PpsI GAGTC 1 cut(s) 904
Ppu21I YACGTR 1 cut(s) 570
PpuMI RGGWCCY 1 cut(s) 370
PscI ACATGT 1 cut(s) 1110
PshBI ATTAAT 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 3 cut(s) 437, 739, 940
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 3 cut(s) 437, 739, 940
PspN4I GGNNCC 6 cut(s) 6, 120, 372, 684, 864, 946
PspPI GGNCC 1 cut(s) 370
PspPPI RGGWCCY 1 cut(s) 370
PsuI RGATCY 4 cut(s) 86, 248, 815, 1363
PsyI GACNNNGTC 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 1298
RsaI GTAC 6 cut(s) 160, 242, 436, 580, 684, 1280
RsaNI GTAC 6 cut(s) 159, 241, 435, 579, 683, 1279
RseI CAYNNNNRTG 1 cut(s) 400
SapI GCTCTTC 1 cut(s) 1127
SaqAI TTAA 5 cut(s) 108, 498, 654, 1026, 1375
SatI GCNGC 4 cut(s) 37, 1138, 1296, 1299
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 1280
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 3 cut(s) 439, 741, 942
SfaNI GCATC 4 cut(s) 157, 222, 349, 404
SgrAI CRCCGGYG 1 cut(s) 770
SinI GGWCC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
SnaBI TACGTA 1 cut(s) 570
Sse9I AATT 7 cut(s) 79, 484, 599, 610, 1265, 1340, 1355
SsiI CCGC 5 cut(s) 36, 39, 320, 1174, 1301
SspMI CTAG 1 cut(s) 347
StyD4I CCNGG 3 cut(s) 437, 739, 940
StyI CCWWGG 3 cut(s) 460, 1241, 1349
TaaI ACNGT 1 cut(s) 1074
TaiI ACGT 3 cut(s) 436, 572, 624
TaqI TCGA 7 cut(s) 71, 211, 732, 823, 1035, 1212, 1334
TasI AATT 7 cut(s) 79, 484, 599, 610, 1265, 1340, 1355
TatI WGTACW 4 cut(s) 158, 240, 578, 1278
TauI GCSGC 1 cut(s) 39
TfiI GAWTC 2 cut(s) 734, 1067
Tru1I TTAA 5 cut(s) 108, 498, 654, 1026, 1375
Tru9I TTAA 5 cut(s) 108, 498, 654, 1026, 1375
TscAI CASTG 2 cut(s) 1045, 1237
TseFI GTSAC 4 cut(s) 268, 646, 773, 1228
TseI GCWGC 3 cut(s) 1137, 1295, 1298
Tsp45I GTSAC 4 cut(s) 268, 646, 773, 1228
TspDTI ATGAA 2 cut(s) 493, 843
TspGWI ACGGA 2 cut(s) 403, 1235
TspRI CASTG 2 cut(s) 1045, 1237
Tth111I GACNNNGTC 1 cut(s) 779
Van91I CCANNNNNTGG 1 cut(s) 461
VpaK11BI GGWCC 1 cut(s) 370
VspI ATTAAT 1 cut(s) 1026
XagI CCTNNNNNAGG 1 cut(s) 939
XapI RAATTY 1 cut(s) 599
XceI RCATGY 1 cut(s) 1114
XmiI GTMKAC 1 cut(s) 1370
XmnI GAANNNNTTC 1 cut(s) 446
XspI CTAG 1 cut(s) 347
ZrmI AGTACT 1 cut(s) 1280
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.