Rh3DG150200

Belongs to the OSBP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0006513
Physical Location & Seq
Reverse (-)
12847251 .. 12852401
5151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG150200.1

Sequence Viewer

Length: 990 bp
ATGCAGAAGTATGTAGGTGCTGACGTCACATCAATGGTGACCCTTCCAGTTCTTATTTTTGAGCCAATGACTATGCTACAGAAAATGGCAGAGTTGATGGAATACTCCCATCTGTTAGATCTGGCAGATGAATGTGAGGATCCATACATGCAATTGGTATATTCTGCATCTTTCTTTATATCTGTTTACTATGCCTTTCAACGCACCTGGAAGCCATTCAACCCCATTCTTGGTGAGACTTACGAAATGGTTAATCATGGTGGCATTACATTTGTAGCAGAACAGGTTAGTCATCACCCTCCTATGAGTGCTGCTCATGCAGAAAATGAGCATTTCATATATGACATAACTTCAAAGGTGAAAACCAAATTTCTAGGGAACTCGATTGATATCTATCCAGTTGGAAGGACACGTTTGACCCTCAAGAAGTCTGGTGTAGTCTTAGATTTGGCACCCCCGCCAACAAAAGTCAACAACTTAATTTTTGGAAGGACATGGGTTGATTCACCAGGAGATATGGTTTTGACCAATTTGACCACAGGGGATAAAGTTGTGCTATATTTTCAACCATGCGGCTGGTTTGGAGCTGGTCGCTATGAAGTTGATGGATATGTTTATAATTCGACCGAGGAACCCAAGATATTGATGACTGGAAAGTGGAATGAGTCAATGAGTTATCAACCCTGTGACGCAGAAGGTGAACCACTTCCTGGCACTGAATTGAAAGAGTCTTGGAAAGTTGCTGAAGTTCCAGAAAATGACAAATTCCAGTACACATATTTTGCGCATAAAATAAACAGCTTCGACACTGCTCCTAAGAAGTTACTGGCATCAGATTCTCGGTTGCGTCCTGATAGGTTTGCACTGGAGCAGGGTGACATACCTAAAGCTGGCTTTGAAAAGAGCAGGTCCCCCATCTCTCTCTCTTTCACACACACACACACACACACACAACACACTACAAACACAAACCAACACACGCTACAGTAA

Protein Analysis

329

Amino Acids

37.33

Weight (kDa)

5.64

Isoelectric Point (pI)

28.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxysterol_BP PF01237 1 - 303 5e-94 Oxysterol-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 618
AatII GACGTC 1 cut(s) 27
Acc16I TGCGCA 1 cut(s) 786
Acc36I ACCTGC 1 cut(s) 897
AccB1I GGYRCC 1 cut(s) 451
AccB7I CCANNNNNTGG 1 cut(s) 710
AciI CCGC 2 cut(s) 458, 573
AclWI GGATC 2 cut(s) 134, 147
AcsI RAATTY 2 cut(s) 368, 764
AcuI CTGAAG 1 cut(s) 765
AcyI GRCGYC 1 cut(s) 24
AfaI GTAC 1 cut(s) 773
AfiI CCNNNNNNNGG 3 cut(s) 230, 710, 890
AflIII ACRYGT 1 cut(s) 410
AgsI TTSAA 6 cut(s) 200, 220, 354, 566, 724, 899
AjnI CCWGG 3 cut(s) 206, 508, 709
AluBI AGCT 3 cut(s) 587, 801, 890
AluI AGCT 3 cut(s) 587, 801, 890
Alw26I GTCTC 1 cut(s) 230
AlwI GGATC 2 cut(s) 134, 147
ApeKI GCWGC 1 cut(s) 311
ApoI RAATTY 2 cut(s) 368, 764
Asp700I GAANNNNTTC 2 cut(s) 215, 705
AspLEI GCGC 1 cut(s) 787
AspS9I GGNCC 1 cut(s) 909
AsuHPI GGTGA 7 cut(s) 49, 245, 287, 370, 498, 710, 887
AvaII GGWCC 1 cut(s) 909
BamHI GGATCC 1 cut(s) 139
BanI GGYRCC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 298
BccI CCATC 4 cut(s) 91, 117, 599, 923
BciT130I CCWGG 3 cut(s) 208, 510, 711
BcoDI GTCTC 1 cut(s) 230
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 2 cut(s) 77, 983
BfuAI ACCTGC 1 cut(s) 897
BglII AGATCT 1 cut(s) 118
BisI GCNGC 2 cut(s) 312, 574
BlsI GCNGC 2 cut(s) 313, 575
Bme1390I CCNGG 3 cut(s) 208, 510, 711
Bme18I GGWCC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 909
BmiI GGNNCC 4 cut(s) 141, 453, 633, 911
BmrFI CCNGG 3 cut(s) 208, 510, 711
BmsI GCATC 2 cut(s) 176, 839
BplI GAGNNNNNCTC 2 cut(s) 298, 330
BpmI CTGGAG 1 cut(s) 887
BpuEI CTTGAG 1 cut(s) 407
BsaBI GATNNNNATC 2 cut(s) 389, 393
BsaHI GRCGYC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 627
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 230, 710, 890
Bse1I ACTGG 6 cut(s) 47, 398, 655, 769, 831, 870
Bse8I GATNNNNATC 2 cut(s) 389, 393
BseBI CCWGG 3 cut(s) 208, 510, 711
BseDI CCNNGG 1 cut(s) 627
BseJI GATNNNNATC 2 cut(s) 389, 393
BseLI CCNNNNNNNGG 3 cut(s) 230, 710, 890
BseNI ACTGG 6 cut(s) 47, 398, 655, 769, 831, 870
BseXI GCAGC 1 cut(s) 298
Bsh1285I CGRYCG 1 cut(s) 627
BshNI GGYRCC 1 cut(s) 451
BsiEI CGRYCG 1 cut(s) 627
BslFI GGGAC 1 cut(s) 895
BslI CCNNNNNNNGG 3 cut(s) 230, 710, 890
BsmAI GTCTC 1 cut(s) 230
BsmFI GGGAC 1 cut(s) 895
Bsp143I GATC 2 cut(s) 118, 139
BspACI CCGC 2 cut(s) 458, 573
BspLI GGNNCC 4 cut(s) 141, 453, 633, 911
BspMI ACCTGC 1 cut(s) 897
BspPI GGATC 2 cut(s) 134, 147
BspT107I GGYRCC 1 cut(s) 451
BsrI ACTGG 6 cut(s) 47, 398, 655, 769, 831, 870
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 2 cut(s) 118, 139
BssNI GRCGYC 1 cut(s) 24
Bst2UI CCWGG 3 cut(s) 208, 510, 711
Bst4CI ACNGT 1 cut(s) 987
BstACI GRCGYC 1 cut(s) 24
BstC8I GCNNGC 1 cut(s) 892
BstDEI CTNAG 2 cut(s) 442, 816
BstEII GGTNACC 1 cut(s) 37
BstHHI GCGC 1 cut(s) 787
BstKTI GATC 2 cut(s) 121, 142
BstMAI GTCTC 1 cut(s) 230
BstMBI GATC 2 cut(s) 118, 139
BstMCI CGRYCG 1 cut(s) 627
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 3 cut(s) 208, 510, 711
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 37
BstSCI CCNGG 3 cut(s) 206, 508, 709
BstSFI CTRYAG 2 cut(s) 77, 983
BstV1I GCAGC 1 cut(s) 298
BstX2I RGATCY 2 cut(s) 118, 139
BstXI CCANNNNNNTGG 1 cut(s) 576
BstYI RGATCY 2 cut(s) 118, 139
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 3 cut(s) 714, 807, 863
BveI ACCTGC 1 cut(s) 897
Cac8I GCNNGC 1 cut(s) 892
CfoI GCGC 1 cut(s) 787
Cfr13I GGNCC 1 cut(s) 909
CseI GACGC 2 cut(s) 698, 836
Csp6I GTAC 1 cut(s) 772
CviAII CATG 5 cut(s) 148, 257, 317, 495, 570
CviJI RGCY 7 cut(s) 64, 214, 576, 587, 801, 890, 894
CviKI_1 RGCY 7 cut(s) 64, 214, 576, 587, 801, 890, 894
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 2 cut(s) 442, 816
DpnI GATC 2 cut(s) 120, 141
DpnII GATC 2 cut(s) 118, 139
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 909
Eco57I CTGAAG 1 cut(s) 765
Eco91I GGTNACC 1 cut(s) 37
EcoO109I RGGNCCY 1 cut(s) 909
EcoO65I GGTNACC 1 cut(s) 37
EcoRII CCWGG 3 cut(s) 206, 508, 709
EcoRV GATATC 1 cut(s) 391
FaeI CATG 5 cut(s) 151, 260, 320, 498, 573
FaqI GGGAC 1 cut(s) 895
FatI CATG 5 cut(s) 147, 256, 316, 494, 569
FauI CCCGC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 312, 574
Fsp4HI GCNGC 2 cut(s) 312, 574
FspBI CTAG 1 cut(s) 374
FspI TGCGCA 1 cut(s) 786
GlaI GCGC 1 cut(s) 786
GluI GCNGC 2 cut(s) 312, 574
GsuI CTGGAG 1 cut(s) 887
HgaI GACGC 2 cut(s) 698, 836
HhaI GCGC 1 cut(s) 787
Hin1I GRCGYC 1 cut(s) 24
Hin1II CATG 5 cut(s) 151, 260, 320, 498, 573
Hin6I GCGC 1 cut(s) 785
HinP1I GCGC 1 cut(s) 785
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HinfI GANTC 4 cut(s) 503, 665, 728, 836
HphI GGTGA 7 cut(s) 49, 245, 287, 370, 498, 710, 887
Hpy166II GTNNAC 4 cut(s) 187, 472, 701, 774
Hpy188I TCNGA 1 cut(s) 835
Hpy188III TCNNGA 3 cut(s) 424, 752, 851
Hpy8I GTNNAC 4 cut(s) 187, 472, 701, 774
HpyAV CCTTC 4 cut(s) 53, 399, 483, 689
HpyCH4III ACNGT 1 cut(s) 987
HpyCH4IV ACGT 2 cut(s) 24, 412
HpyCH4V TGCA 5 cut(s) 4, 151, 167, 320, 863
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 2 cut(s) 442, 816
HpySE526I ACGT 2 cut(s) 24, 412
Hsp92I GRCGYC 1 cut(s) 24
Hsp92II CATG 5 cut(s) 151, 260, 320, 498, 573
HspAI GCGC 1 cut(s) 785
Kzo9I GATC 2 cut(s) 118, 139
LmnI GCTCC 3 cut(s) 584, 817, 868
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 2 cut(s) 176, 839
MaeI CTAG 1 cut(s) 374
MaeII ACGT 2 cut(s) 24, 412
MaeIII GTNAC 5 cut(s) 25, 37, 686, 822, 875
MalI GATC 2 cut(s) 120, 141
MboI GATC 2 cut(s) 118, 139
MfeI CAATTG 1 cut(s) 152
MflI RGATCY 2 cut(s) 118, 139
MluCI AATT 7 cut(s) 152, 368, 480, 529, 619, 719, 764
MlyI GAGTC 2 cut(s) 674, 737
MmeI TCCRAC 1 cut(s) 382
MnlI CCTC 4 cut(s) 130, 309, 431, 622
MroXI GAANNNNTTC 2 cut(s) 215, 705
MseI TTAA 2 cut(s) 252, 479
MslI CAYNNNNRTG 1 cut(s) 32
MspR9I CCNGG 3 cut(s) 208, 510, 711
MunI CAATTG 1 cut(s) 152
MvaI CCWGG 3 cut(s) 208, 510, 711
MwoI GCNNNNNNNGC 1 cut(s) 317
NdeII GATC 2 cut(s) 118, 139
NlaIII CATG 5 cut(s) 151, 260, 320, 498, 573
NlaIV GGNNCC 4 cut(s) 141, 453, 633, 911
NmuCI GTSAC 4 cut(s) 25, 37, 686, 875
NsbI TGCGCA 1 cut(s) 786
NspI RCATGY 1 cut(s) 151
PdmI GAANNNNTTC 2 cut(s) 215, 705
PfeI GAWTC 2 cut(s) 503, 836
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 2 cut(s) 313, 575
PleI GAGTC 2 cut(s) 673, 736
PpsI GAGTC 2 cut(s) 673, 736
PpuMI RGGWCCY 1 cut(s) 909
PsiI TTATAA 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 909
Psp6I CCWGG 3 cut(s) 206, 508, 709
PspEI GGTNACC 1 cut(s) 37
PspGI CCWGG 3 cut(s) 206, 508, 709
PspN4I GGNNCC 4 cut(s) 141, 453, 633, 911
PspPI GGNCC 1 cut(s) 909
PspPPI RGGWCCY 1 cut(s) 909
PsuI RGATCY 2 cut(s) 118, 139
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 1 cut(s) 32
SaqAI TTAA 2 cut(s) 252, 479
SatI GCNGC 2 cut(s) 312, 574
Sau3AI GATC 2 cut(s) 118, 139
Sau96I GGNCC 1 cut(s) 909
SchI GAGTC 2 cut(s) 674, 737
ScrFI CCNGG 3 cut(s) 208, 510, 711
SfaNI GCATC 2 cut(s) 176, 839
SfcI CTRYAG 2 cut(s) 77, 983
SinI GGWCC 1 cut(s) 909
SmiMI CAYNNNNRTG 1 cut(s) 32
SmlI CTYRAG 1 cut(s) 422
SmoI CTYRAG 1 cut(s) 422
Sse9I AATT 7 cut(s) 152, 368, 480, 529, 619, 719, 764
SsiI CCGC 2 cut(s) 458, 573
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 3 cut(s) 206, 508, 709
TaaI ACNGT 1 cut(s) 987
TaiI ACGT 2 cut(s) 27, 415
TaqI TCGA 3 cut(s) 383, 623, 804
TaqII GACCGA 1 cut(s) 641
TasI AATT 7 cut(s) 152, 368, 480, 529, 619, 719, 764
TatI WGTACW 1 cut(s) 771
TauI GCSGC 1 cut(s) 576
TfiI GAWTC 2 cut(s) 503, 836
Tru1I TTAA 2 cut(s) 252, 479
Tru9I TTAA 2 cut(s) 252, 479
TscAI CASTG 3 cut(s) 721, 814, 870
TseFI GTSAC 4 cut(s) 25, 37, 686, 875
TseI GCWGC 1 cut(s) 311
Tsp45I GTSAC 4 cut(s) 25, 37, 686, 875
TspDTI ATGAA 3 cut(s) 144, 325, 612
TspRI CASTG 3 cut(s) 721, 814, 870
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 909
XapI RAATTY 2 cut(s) 368, 764
XceI RCATGY 1 cut(s) 151
XmnI GAANNNNTTC 2 cut(s) 215, 705
XspI CTAG 1 cut(s) 374
ZraI GACGTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.