AT3G10250

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003012
Physical Location & Seq
Reverse (-)
3167922 .. 3170957
3036 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10250.3

Sequence Viewer

Length: 975 bp
ATGTCGAGTGGAACAGTGAGAAGGGTTTCACGTCAAGATATACAACTGGTTCAAAACCTTATAGAACGATGCCTTCAACTTTACATGAATCAGAAAGAAGTTGTGGATACTTTACTAGAGCAGGCTAAAATCGAGCCTGGGTTCACAGAACTAGTTTGGCAGAAGCTTGAAGAAGAGAATCGGGAGTTTTTCAAAGCTTACTATCTTAGGCTAATGGTTAAACACCAGATAATGGAATTCAACAAGTTGCTTGAGCAGCAGGTTCACCACATGCGTCAGATGCATCCAACTGGTGTTGCTTCTGTCCAAAACACAAATGGTTCTCATCTTCAATCAATGAATCAGAAACAATTATGCTATCCCTCTGAGCACACTGACCAATCTTTGAAGTCTGAAAGTGCACATCATCCAATGGCTTCTAGTCTGTCTAATGCATTCCTCAATGGCTCTTCCACACTCAATACAAACGTGCCCTCTTCTATAAACATATCAACCCATGCTAGGAGAGTTGATGCTTCTCCAAATATGCTCTCATCGCAGACCACAAACATGCCTATGATGCAAGGAATGAATGGAGGAATGATCAAGTCTGAGACTGCCTTTACAAACCCTGCTTCATTCATGTATGGTGGCGAACGGAATGCCCTCGAAGGACATTCGGCAGTTGGGGATACTTCGATTCCTAATTTCAGTAACGAGTCCAACAACCAACCCCTCAGTGATCCGCTTCTTGAAGCAGAAGCTTCTACTTTTGGGTTCTTAGGTCAAATTCCTCGTAACTTTAGCCTCTCTGATTTGACTGCTGATTTTTCTCAGAGCTCAGAGATTCTGGAGAGCTACGATAGATCACCCTTCCTTGTGCCGAATGCTGAAAACTTCCTGGACTCCCGTGACAGAGGAGAATATCAAGGAGACAACAAGAGATTGGATACCATATCTGAAGGTTTCAGTTACGATAACATCGGGAGCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

324

Amino Acids

36.38

Weight (kDa)

5.16

Isoelectric Point (pI)

53.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 17 - 68 3e-26 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 232
AciI CCGC 1 cut(s) 725
AclWI GGATC 1 cut(s) 716
AcsI RAATTY 2 cut(s) 236, 768
AcuI CTGAAG 1 cut(s) 960
AfiI CCNNNNNNNGG 2 cut(s) 232, 501
AgsI TTSAA 8 cut(s) 53, 77, 170, 193, 241, 332, 388, 734
AhlI ACTAGT 1 cut(s) 151
AjiI CACGTC 1 cut(s) 32
AjnI CCWGG 2 cut(s) 136, 879
AluBI AGCT 5 cut(s) 166, 197, 743, 819, 837
AluI AGCT 5 cut(s) 166, 197, 743, 819, 837
Alw21I GWGCWC 3 cut(s) 372, 403, 821
Alw26I GTCTC 2 cut(s) 587, 906
Alw44I GTGCAC 1 cut(s) 399
AlwI GGATC 1 cut(s) 716
ApaLI GTGCAC 1 cut(s) 399
ApeKI GCWGC 1 cut(s) 256
ApoI RAATTY 2 cut(s) 236, 768
Asp700I GAANNNNTTC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 257, 840
BaeGI GKGCMC 2 cut(s) 403, 474
BanII GRGCYC 1 cut(s) 821
Bbv12I GWGCWC 3 cut(s) 372, 403, 821
BbvI GCAGC 1 cut(s) 268
BcgI CGANNNNNNTGC 2 cut(s) 623, 657
BciT130I CCWGG 2 cut(s) 138, 881
BciVI GTATCC 3 cut(s) 100, 664, 922
BclI TGATCA 1 cut(s) 582
BcoDI GTCTC 2 cut(s) 587, 906
BcuI ACTAGT 1 cut(s) 151
BfaI CTAG 4 cut(s) 116, 152, 420, 501
BfuAI ACCTGC 1 cut(s) 250
BfuI GTATCC 3 cut(s) 100, 664, 922
BisI GCNGC 1 cut(s) 257
BlsI GCNGC 1 cut(s) 258
Bme1390I CCNGG 2 cut(s) 138, 881
BmgBI CACGTC 1 cut(s) 32
BmrFI CCNGG 2 cut(s) 138, 881
BmsI GCATC 5 cut(s) 59, 270, 292, 502, 549
BpmI CTGGAG 1 cut(s) 851
BpuEI CTTGAG 1 cut(s) 272
BsaJI CCNNGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 232, 501
Bse1I ACTGG 2 cut(s) 51, 295
BseBI CCWGG 2 cut(s) 138, 881
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 2 cut(s) 283, 406
BseLI CCNNNNNNNGG 2 cut(s) 232, 501
BseMII CTCAG 5 cut(s) 357, 582, 730, 827, 834
BseNI ACTGG 2 cut(s) 51, 295
BseRI GAGGAG 1 cut(s) 912
BseSI GKGCMC 2 cut(s) 403, 474
BseXI GCAGC 1 cut(s) 268
BsiHKAI GWGCWC 3 cut(s) 372, 403, 821
BslI CCNNNNNNNGG 2 cut(s) 232, 501
BsmAI GTCTC 2 cut(s) 587, 906
BsmI GAATGC 3 cut(s) 434, 646, 871
Bsp1286I GDGCHC 4 cut(s) 372, 403, 474, 821
Bsp143I GATC 3 cut(s) 582, 721, 845
BspACI CCGC 1 cut(s) 725
BspCNI CTCAG 5 cut(s) 358, 583, 729, 826, 833
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 716
BspQI GCTCTTC 1 cut(s) 454
BsrI ACTGG 2 cut(s) 51, 295
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 3 cut(s) 582, 721, 845
Bst2UI CCWGG 2 cut(s) 138, 881
Bst4CI ACNGT 1 cut(s) 16
Bst6I CTCTTC 3 cut(s) 168, 454, 481
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 7 cut(s) 206, 366, 591, 716, 760, 813, 820
BstF5I GGATG 2 cut(s) 283, 406
BstKTI GATC 3 cut(s) 585, 724, 848
BstMAI GTCTC 2 cut(s) 587, 906
BstMBI GATC 3 cut(s) 582, 721, 845
BstMWI GCNNNNNNNGC 4 cut(s) 256, 280, 535, 559
BstNI CCWGG 2 cut(s) 138, 881
BstNSI RCATGY 2 cut(s) 274, 553
BstSCI CCNGG 2 cut(s) 136, 879
BstSLI GKGCMC 2 cut(s) 403, 474
BstV1I GCAGC 1 cut(s) 268
BsuI GTATCC 3 cut(s) 100, 664, 922
BtgZI GCGATG 1 cut(s) 519
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 2 cut(s) 283, 406
BtsIMutI CAGTG 3 cut(s) 21, 372, 724
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 123
CseI GACGC 1 cut(s) 263
CviAII CATG 5 cut(s) 85, 271, 497, 550, 622
DdeI CTNAG 7 cut(s) 206, 366, 591, 716, 760, 813, 820
DpnI GATC 3 cut(s) 584, 723, 847
DpnII GATC 3 cut(s) 582, 721, 845
Eam1104I CTCTTC 3 cut(s) 168, 454, 481
EarI CTCTTC 3 cut(s) 168, 454, 481
Ecl136II GAGCTC 1 cut(s) 819
Eco24I GRGCYC 1 cut(s) 821
Eco53kI GAGCTC 1 cut(s) 819
Eco57I CTGAAG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 819
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 136, 879
EcoT22I ATGCAT 2 cut(s) 285, 436
EcoT38I GRGCYC 1 cut(s) 821
FaeI CATG 5 cut(s) 88, 274, 500, 553, 625
FatI CATG 5 cut(s) 84, 270, 496, 549, 621
FbaI TGATCA 1 cut(s) 582
Fnu4HI GCNGC 1 cut(s) 257
FokI GGATG 2 cut(s) 270, 393
FriOI GRGCYC 1 cut(s) 821
Fsp4HI GCNGC 1 cut(s) 257
FspBI CTAG 4 cut(s) 116, 152, 420, 501
GluI GCNGC 1 cut(s) 257
GsuI CTGGAG 1 cut(s) 851
HgaI GACGC 1 cut(s) 263
Hin1II CATG 5 cut(s) 88, 274, 500, 553, 625
HindIII AAGCTT 3 cut(s) 164, 195, 741
HinfI GANTC 7 cut(s) 88, 178, 340, 679, 698, 826, 884
HphI GGTGA 2 cut(s) 257, 840
Hpy166II GTNNAC 3 cut(s) 144, 265, 401
Hpy188III TCNNGA 5 cut(s) 35, 182, 731, 830, 964
Hpy8I GTNNAC 3 cut(s) 144, 265, 401
HpyAV CCTTC 5 cut(s) 15, 83, 644, 862, 935
HpyCH4III ACNGT 1 cut(s) 16
HpyCH4IV ACGT 2 cut(s) 31, 468
HpyCH4V TGCA 4 cut(s) 283, 401, 434, 562
HpyF10VI GCNNNNNNNGC 4 cut(s) 256, 280, 535, 559
HpyF3I CTNAG 7 cut(s) 206, 366, 591, 716, 760, 813, 820
HpySE526I ACGT 2 cut(s) 31, 468
Hsp92II CATG 5 cut(s) 88, 274, 500, 553, 625
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 3 cut(s) 582, 721, 845
LguI GCTCTTC 1 cut(s) 454
LmnI GCTCC 1 cut(s) 966
Lsp1109I GCAGC 1 cut(s) 268
LweI GCATC 5 cut(s) 59, 270, 292, 502, 549
MaeI CTAG 4 cut(s) 116, 152, 420, 501
MaeII ACGT 2 cut(s) 31, 468
MaeIII GTNAC 4 cut(s) 692, 776, 890, 950
MalI GATC 3 cut(s) 584, 723, 847
MboI GATC 3 cut(s) 582, 721, 845
MboII GAAGA 5 cut(s) 182, 185, 320, 441, 468
MhlI GDGCHC 4 cut(s) 372, 403, 474, 821
MluCI AATT 4 cut(s) 236, 350, 685, 768
MlyI GAGTC 2 cut(s) 707, 878
MmeI TCCRAC 2 cut(s) 311, 726
MnlI CCTC 9 cut(s) 373, 449, 484, 569, 656, 725, 783, 797, 890
Mph1103I ATGCAT 2 cut(s) 285, 436
MroXI GAANNNNTTC 1 cut(s) 25
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 2 cut(s) 548, 554
MspR9I CCNGG 2 cut(s) 138, 881
Mva1269I GAATGC 3 cut(s) 434, 646, 871
MvaI CCWGG 2 cut(s) 138, 881
MwoI GCNNNNNNNGC 4 cut(s) 256, 280, 535, 559
NdeII GATC 3 cut(s) 582, 721, 845
NlaIII CATG 5 cut(s) 88, 274, 500, 553, 625
NmuCI GTSAC 1 cut(s) 890
NsiI ATGCAT 2 cut(s) 285, 436
NspI RCATGY 2 cut(s) 274, 553
PciSI GCTCTTC 1 cut(s) 454
PctI GAATGC 3 cut(s) 434, 646, 871
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 5 cut(s) 88, 178, 340, 679, 826
PflMI CCANNNNNTGG 1 cut(s) 232
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 1 cut(s) 258
PleI GAGTC 2 cut(s) 706, 878
PpsI GAGTC 2 cut(s) 706, 878
Psp124BI GAGCTC 1 cut(s) 821
Psp6I CCWGG 2 cut(s) 136, 879
PspGI CCWGG 2 cut(s) 136, 879
RseI CAYNNNNRTG 2 cut(s) 548, 554
SacI GAGCTC 1 cut(s) 821
SapI GCTCTTC 1 cut(s) 454
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 257
Sau3AI GATC 3 cut(s) 582, 721, 845
SchI GAGTC 2 cut(s) 707, 878
ScrFI CCNGG 2 cut(s) 138, 881
SduI GDGCHC 4 cut(s) 372, 403, 474, 821
SfaNI GCATC 5 cut(s) 59, 270, 292, 502, 549
SmiMI CAYNNNNRTG 2 cut(s) 548, 554
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SpeI ACTAGT 1 cut(s) 151
Sse9I AATT 4 cut(s) 236, 350, 685, 768
SsiI CCGC 1 cut(s) 725
SspMI CTAG 4 cut(s) 116, 152, 420, 501
SstI GAGCTC 1 cut(s) 821
StyD4I CCNGG 2 cut(s) 136, 879
TaaI ACNGT 1 cut(s) 16
TaiI ACGT 2 cut(s) 34, 471
TaqI TCGA 4 cut(s) 5, 132, 648, 677
TasI AATT 4 cut(s) 236, 350, 685, 768
TfiI GAWTC 5 cut(s) 88, 178, 340, 679, 826
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 3 cut(s) 21, 379, 724
TseFI GTSAC 1 cut(s) 890
TseI GCWGC 1 cut(s) 256
Tsp45I GTSAC 1 cut(s) 890
TspDTI ATGAA 5 cut(s) 101, 353, 584, 606, 610
TspGWI ACGGA 1 cut(s) 652
TspRI CASTG 3 cut(s) 21, 379, 724
Van91I CCANNNNNTGG 1 cut(s) 232
VneI GTGCAC 1 cut(s) 399
XapI RAATTY 2 cut(s) 236, 768
XceI RCATGY 2 cut(s) 274, 553
XcmI CCANNNNNNNNNTGG 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 25
XspI CTAG 4 cut(s) 116, 152, 420, 501
Zsp2I ATGCAT 2 cut(s) 285, 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.