MD12G1093600.v1.1

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003012
Physical Location & Seq
Forward (+)
12275381 .. 12282276
6896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1093600.v1.1.491

Sequence Viewer

Length: 972 bp
ATGTCTAGTGGATCCATTAGACGGGTCTCACGTGAAGATATACAACTGGTGCAAAACCTTATCGAGCGATGTCTCCAGCTTTACATGAACCAGAAGGAAGTGGTAGATACTTTACTGGATCAGGCAAAAATAGAGCCTGGTTTCACAGAACTTGTTTGGCAGAAACTTGAGGAAGAGAATCGGGAATTCTTCAGGGCGTATTATTTAAGATTGATGGTGAAGCACCAAATAATGGAATATAACAACTTGCTTAAGCAACAGGTGGAATTGATGAGTCAGTTACACTCAAGTGGAGTCACTTCAATATCTAGTTCCAATGGATCTCACATTTCATCAATGCGCCAAAACTCAACATGCTATGCACCAGAGCATGTAGGAACAGCGCTGAAGACAGAAAATATACACCACCAAGTTGGTTCTTGCCTACATAATGCTTTTACTAATGGTGGATCTTCATTGCACATCAGTATGCACAATGCTGGTAAAATGTCTGCTCATACCGGTAGGATTGATGTCCAACCAAACATGCTTTCGAACCAGATCTCCAACATAGGTCTGATGCATGGAATGAATGGGGGAATTGTCAAATCAGAAGTTGGATATTCAGGCAGTTCGTACATGTTTAGTGCTGATGGAAACATCCTGGAAACCCGGCCCGCTATTGGAGATGCTTCTGTTGCAGCATTCAATAGCGTAGAGTCCAATTCACAGTCCCTAAATGAATCTCTGCTTGATGCAGACAGTTCATCATTTGGTCTTTTAGGTCAGATTCCTCGAATCTTGAGCTTTTCGGATCTTGCAACTGACTTTTCTCAGAGTTCAGACATACTGGAGAGCTATCCTAGATCACCCTTCTTGGCTACAGATAATAACAACTTCCTGGATTCTCGTGACAGAGAACATCAAGGGGACAATAACAGATTGGACACCGTCTCAGAAGGTGTAAGTTATGAAGATTTTGGCAGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

324

Amino Acids

35.77

Weight (kDa)

5.12

Isoelectric Point (pI)

59.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 17 - 68 3.4e-26 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 232
AciI CCGC 1 cut(s) 657
AclWI GGATC 6 cut(s) 6, 19, 126, 328, 457, 801
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 175, 407
AcvI CACGTG 1 cut(s) 32
AfaI GTAC 1 cut(s) 617
AfeI AGCGCT 1 cut(s) 384
AfiI CCNNNNNNNGG 3 cut(s) 21, 232, 662
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 618
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 2 cut(s) 303, 688
AjnI CCWGG 3 cut(s) 136, 642, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AleI CACNNNNGTG 1 cut(s) 288
AloI GAACNNNNNNTCC 2 cut(s) 527, 559
AluBI AGCT 3 cut(s) 79, 786, 837
AluI AGCT 3 cut(s) 79, 786, 837
Alw26I GTCTC 3 cut(s) 31, 77, 937
AlwI GGATC 6 cut(s) 6, 19, 126, 328, 457, 801
Aor51HI AGCGCT 1 cut(s) 384
AoxI GGCC 1 cut(s) 653
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 1 cut(s) 185
AsiGI ACCGGT 1 cut(s) 500
AspLEI GCGC 2 cut(s) 342, 385
AspS9I GGNCC 1 cut(s) 654
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 2 cut(s) 229, 840
AsuII TTCGAA 1 cut(s) 533
BamHI GGATCC 1 cut(s) 11
BauI CACGAG 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 1 cut(s) 692
BccI CCATC 2 cut(s) 208, 626
BciT130I CCWGG 3 cut(s) 138, 644, 881
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 31, 77, 937
BfaI CTAG 3 cut(s) 6, 309, 843
BfmI CTRYAG 1 cut(s) 861
BfoI RGCGCY 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 251
BglII AGATCT 1 cut(s) 540
BisI GCNGC 1 cut(s) 681
BlsI GCNGC 1 cut(s) 682
Bme1390I CCNGG 4 cut(s) 138, 644, 652, 881
BmgT120I GGNCC 1 cut(s) 654
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 4 cut(s) 138, 644, 652, 881
BmsI GCATC 3 cut(s) 549, 658, 724
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 2 cut(s) 59, 851
Bpu14I TTCGAA 1 cut(s) 533
BpuEI CTTGAG 3 cut(s) 188, 271, 802
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 31
BsaWI WCCGGW 1 cut(s) 500
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 232, 662
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 3 cut(s) 51, 120, 834
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 3 cut(s) 138, 644, 881
BseGI GGATG 1 cut(s) 639
BseLI CCNNNNNNNGG 3 cut(s) 21, 232, 662
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 2 cut(s) 827, 948
BseNI ACTGG 3 cut(s) 51, 120, 834
BseXI GCAGC 1 cut(s) 692
BshFI GGCC 1 cut(s) 655
BshTI ACCGGT 1 cut(s) 500
BsiSI CCGG 2 cut(s) 501, 652
BslFI GGGAC 2 cut(s) 697, 923
BslI CCNNNNNNNGG 3 cut(s) 21, 232, 662
BsmAI GTCTC 3 cut(s) 31, 77, 937
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 2 cut(s) 697, 923
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 1 cut(s) 655
Bso31I GGTCTC 1 cut(s) 31
Bsp119I TTCGAA 1 cut(s) 533
Bsp143I GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
BspACI CCGC 1 cut(s) 657
BspANI GGCC 1 cut(s) 655
BspCNI CTCAG 2 cut(s) 826, 947
BspLI GGNNCC 1 cut(s) 13
BspPI GGATC 6 cut(s) 6, 19, 126, 328, 457, 801
BspT104I TTCGAA 1 cut(s) 533
BspTI CTTAAG 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 455
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 3 cut(s) 51, 120, 834
BssAI RCCGGY 1 cut(s) 500
BssMI GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
BssSI CACGAG 1 cut(s) 888
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 3 cut(s) 138, 644, 881
Bst4CI ACNGT 3 cut(s) 711, 743, 931
Bst6I CTCTTC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 32
BstBI TTCGAA 1 cut(s) 533
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 2 cut(s) 813, 934
BstF5I GGATG 1 cut(s) 639
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 2 cut(s) 342, 385
BstKTI GATC 7 cut(s) 14, 121, 323, 452, 543, 796, 848
BstMAI GTCTC 3 cut(s) 31, 77, 937
BstMBI GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNI CCWGG 3 cut(s) 138, 644, 881
BstNSI RCATGY 4 cut(s) 357, 374, 529, 622
BstSCI CCNGG 4 cut(s) 136, 642, 650, 879
BstSFI CTRYAG 1 cut(s) 861
BstV1I GCAGC 1 cut(s) 692
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 5 cut(s) 11, 320, 449, 540, 793
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 5 cut(s) 11, 320, 449, 540, 793
BsuRI GGCC 1 cut(s) 655
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 639
BtsI GCAGTG 1 cut(s) 970
BtsIMutI CAGTG 1 cut(s) 970
Cac8I GCNNGC 1 cut(s) 657
CfoI GCGC 2 cut(s) 342, 385
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 1 cut(s) 654
Csp6I GTAC 1 cut(s) 616
CspAI ACCGGT 1 cut(s) 500
CviAII CATG 6 cut(s) 85, 354, 371, 526, 563, 619
CviJI RGCY 6 cut(s) 79, 136, 655, 786, 837, 860
CviKI_1 RGCY 6 cut(s) 79, 136, 655, 786, 837, 860
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 2 cut(s) 813, 934
DpnI GATC 7 cut(s) 13, 120, 322, 451, 542, 795, 847
DpnII GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 31
Eco47III AGCGCT 1 cut(s) 384
Eco57I CTGAAG 2 cut(s) 175, 407
Eco72I CACGTG 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 3 cut(s) 136, 642, 879
EcoT22I ATGCAT 1 cut(s) 564
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 6 cut(s) 88, 357, 374, 529, 566, 622
FaqI GGGAC 2 cut(s) 697, 923
FatI CATG 6 cut(s) 84, 353, 370, 525, 562, 618
FauI CCCGC 1 cut(s) 664
Fnu4HI GCNGC 1 cut(s) 681
FokI GGATG 1 cut(s) 626
Fsp4HI GCNGC 1 cut(s) 681
FspBI CTAG 3 cut(s) 6, 309, 843
GlaI GCGC 2 cut(s) 341, 384
GluI GCNGC 1 cut(s) 681
GsuI CTGGAG 2 cut(s) 59, 851
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 1 cut(s) 655
HapII CCGG 2 cut(s) 501, 652
HhaI GCGC 2 cut(s) 342, 385
Hin1II CATG 6 cut(s) 88, 357, 374, 529, 566, 622
Hin6I GCGC 2 cut(s) 340, 383
HinP1I GCGC 2 cut(s) 340, 383
HinfI GANTC 8 cut(s) 178, 274, 294, 698, 722, 769, 777, 884
HpaII CCGG 2 cut(s) 501, 652
HphI GGTGA 2 cut(s) 229, 840
Hpy188I TCNGA 7 cut(s) 558, 592, 768, 793, 816, 823, 937
Hpy188III TCNNGA 3 cut(s) 182, 781, 890
HpyAV CCTTC 3 cut(s) 88, 862, 932
HpyCH4III ACNGT 3 cut(s) 711, 743, 931
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 8 cut(s) 52, 362, 460, 472, 562, 680, 737, 800
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 2 cut(s) 813, 934
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 6 cut(s) 88, 357, 374, 529, 566, 622
HspAI GCGC 2 cut(s) 340, 383
Kzo9I GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
Lsp1109I GCAGC 1 cut(s) 692
LweI GCATC 3 cut(s) 549, 658, 724
MaeI CTAG 3 cut(s) 6, 309, 843
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 3 cut(s) 279, 295, 890
MalI GATC 7 cut(s) 13, 120, 322, 451, 542, 795, 847
MboI GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
MboII GAAGA 6 cut(s) 47, 181, 185, 400, 444, 965
MflI RGATCY 5 cut(s) 11, 320, 449, 540, 793
MluCI AATT 4 cut(s) 185, 266, 579, 703
MlyI GAGTC 3 cut(s) 283, 303, 707
MmeI TCCRAC 3 cut(s) 541, 570, 577
MnlI CCTC 2 cut(s) 163, 783
Mph1103I ATGCAT 1 cut(s) 564
MseI TTAA 2 cut(s) 206, 252
MslI CAYNNNNRTG 2 cut(s) 288, 467
MspCI CTTAAG 1 cut(s) 251
MspI CCGG 2 cut(s) 501, 652
MspR9I CCNGG 4 cut(s) 138, 644, 652, 881
Mva1269I GAATGC 1 cut(s) 683
MvaI CCWGG 3 cut(s) 138, 644, 881
MwoI GCNNNNNNNGC 1 cut(s) 677
NciI CCSGG 1 cut(s) 652
NdeII GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
NlaIII CATG 6 cut(s) 88, 357, 374, 529, 566, 622
NlaIV GGNNCC 1 cut(s) 13
NmuCI GTSAC 2 cut(s) 295, 890
NsiI ATGCAT 1 cut(s) 564
NspI RCATGY 4 cut(s) 357, 374, 529, 622
NspV TTCGAA 1 cut(s) 533
OliI CACNNNNGTG 1 cut(s) 288
PciI ACATGT 1 cut(s) 618
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 5 cut(s) 178, 722, 769, 777, 884
PflFI GACNNNGTC 1 cut(s) 929
PflMI CCANNNNNTGG 1 cut(s) 232
PfoI TCCNGGA 2 cut(s) 642, 879
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 1 cut(s) 682
PleI GAGTC 3 cut(s) 282, 302, 706
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
PpsI GAGTC 3 cut(s) 282, 302, 706
Ppu21I YACGTR 1 cut(s) 32
PscI ACATGT 1 cut(s) 618
Psp6I CCWGG 3 cut(s) 136, 642, 879
PspCI CACGTG 1 cut(s) 32
PspGI CCWGG 3 cut(s) 136, 642, 879
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 1 cut(s) 654
PsuI RGATCY 5 cut(s) 11, 320, 449, 540, 793
PsyI GACNNNGTC 1 cut(s) 929
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 2 cut(s) 288, 467
SaqAI TTAA 2 cut(s) 206, 252
SatI GCNGC 1 cut(s) 681
Sau3AI GATC 7 cut(s) 11, 118, 320, 449, 540, 793, 845
Sau96I GGNCC 1 cut(s) 654
SchI GAGTC 3 cut(s) 283, 303, 707
ScrFI CCNGG 4 cut(s) 138, 644, 652, 881
SetI ASST 9 cut(s) 34, 60, 81, 264, 556, 766, 788, 839, 943
SfaNI GCATC 3 cut(s) 549, 658, 724
SfcI CTRYAG 1 cut(s) 861
SfuI TTCGAA 1 cut(s) 533
SmiMI CAYNNNNRTG 2 cut(s) 288, 467
SmlI CTYRAG 4 cut(s) 167, 251, 286, 781
SmoI CTYRAG 4 cut(s) 167, 251, 286, 781
Sse9I AATT 4 cut(s) 185, 266, 579, 703
SsiI CCGC 1 cut(s) 657
SspMI CTAG 3 cut(s) 6, 309, 843
StyD4I CCNGG 4 cut(s) 136, 642, 650, 879
TaaI ACNGT 3 cut(s) 711, 743, 931
TaiI ACGT 1 cut(s) 34
TaqI TCGA 3 cut(s) 63, 533, 775
TasI AATT 4 cut(s) 185, 266, 579, 703
TfiI GAWTC 5 cut(s) 178, 722, 769, 777, 884
Tru1I TTAA 2 cut(s) 206, 252
Tru9I TTAA 2 cut(s) 206, 252
TscAI CASTG 1 cut(s) 970
TseFI GTSAC 2 cut(s) 295, 890
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 2 cut(s) 295, 890
TspDTI ATGAA 7 cut(s) 101, 321, 444, 584, 735, 735, 966
TspRI CASTG 1 cut(s) 970
Tth111I GACNNNGTC 1 cut(s) 929
Van91I CCANNNNNTGG 1 cut(s) 232
Vha464I CTTAAG 1 cut(s) 251
XapI RAATTY 1 cut(s) 185
XceI RCATGY 4 cut(s) 357, 374, 529, 622
XspI CTAG 3 cut(s) 6, 309, 843
Zsp2I ATGCAT 1 cut(s) 564
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.