FvH4_6g25280

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003012
Physical Location & Seq
Forward (+)
19146002 .. 19152202
6201 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g25280.t4

Sequence Viewer

Length: 975 bp
ATGTCAAGTGGATCTGTTAGACGTGTCTCACGTCAAGATATACAATTGGTGCAAAACCTTATCGAGCGATGCCTTCAGCTTTACATGAATCAGAAGGAAGTGGTGGATACGCTACTGGAGCAAGCAAAAATAGAGCCTGGTTTCACGGAACTTGTTTGGCAGAAGCTTGAGGAAGAGAATCGTGAATTCTTCAGCGCTTATTACCTGAGATTAATGGTAAAGCAACAAATAATAGAGTATAACAGGTTGCTTGAACAACAAGCGCGGTTGATGCACCAGTTACACTCGACTGGAGTTTCCTCAATTCCCACCTCCAATGGATCTCACATTCCATCGATGCATCAAAACTCAACATGCTATCCCCCAGACAGTGTAGGACCAGCATTGAAGACAGAAAACTTACACCATCAAGTTGGTACATGCTTACCGACTCCATTTACTAATGGCGGGTCTTCATTGCACAACAGTATGGAGAATACTCTTAAAATGTCTGCTCATGCCAACAGGATTGATGTCCCATCAAACATGCTTTCAACTCAGAGCTCCAACATGGGGGGGCTGATGCAAGGAATAAACGGGGGAATTATAAAATCTGAAGTTGGATATTCAGGCACGTCTTACATGTATGGTGCTGATGGCAGCAACATGGAAACACGACCCAATATGGGAGATACTTCTGTTGCTCCTTTCAATAGCGCAGAGGCCAATTCACAACCCCTAACTGAATCACTTCTTGATTCGGACACTTCAACATTTGGTTTTTTAACTCAGATTCCTCGAAATTTCAGTCTCTCAGATCTGACAGCAGACTTTTCTCAGAGTTCAGATATACTGGAGAGCTATCCTAGATCACCCTTCTTAGCTACAGATAATGAAAACTTCCTGGATCCTCGTGAAAGGGAACATCAGGGAGACAATAACAGGCTGGACACCATTTCAGAAGGCGTGAGTTATGAAGAGTTTGCCAGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

325

Amino Acids

36.08

Weight (kDa)

4.85

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 17 - 68 6.8e-26 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccII CGCG 1 cut(s) 265
AciI CCGC 2 cut(s) 265, 447
AclWI GGATC 4 cut(s) 19, 328, 881, 894
AcsI RAATTY 2 cut(s) 185, 781
AcuI CTGAAG 3 cut(s) 59, 175, 615
AfaI GTAC 1 cut(s) 418
AfeI AGCGCT 1 cut(s) 196
AfiI CCNNNNNNNGG 2 cut(s) 552, 665
AflIII ACRYGT 2 cut(s) 22, 621
AgsI TTSAA 5 cut(s) 254, 388, 534, 691, 750
AjiI CACGTC 3 cut(s) 23, 32, 615
AjnI CCWGG 2 cut(s) 136, 882
AluBI AGCT 5 cut(s) 79, 166, 543, 840, 863
AluI AGCT 5 cut(s) 79, 166, 543, 840, 863
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 31, 794, 906
AlwI GGATC 4 cut(s) 19, 328, 881, 894
Aor51HI AGCGCT 1 cut(s) 196
AoxI GGCC 1 cut(s) 702
ApeKI GCWGC 1 cut(s) 639
ApoI RAATTY 2 cut(s) 185, 781
ArsI GACNNNNNNTTYG 2 cut(s) 772, 804
AseI ATTAAT 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 3 cut(s) 197, 265, 698
AspS9I GGNCC 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 843
AvaII GGWCC 1 cut(s) 377
BaeI ACNNNNGTAYC 2 cut(s) 663, 696
BamHI GGATCC 1 cut(s) 886
BanII GRGCYC 1 cut(s) 545
BauI CACGAG 1 cut(s) 891
BbsI GAAGAC 2 cut(s) 395, 444
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 651
BccI CCATC 4 cut(s) 340, 414, 526, 629
BciT130I CCWGG 2 cut(s) 138, 884
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 3 cut(s) 31, 794, 906
BfaI CTAG 1 cut(s) 846
BfmI CTRYAG 1 cut(s) 864
BfoI RGCGCY 1 cut(s) 198
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 796
BisI GCNGC 1 cut(s) 640
BlsI GCNGC 1 cut(s) 641
Bme1390I CCNGG 2 cut(s) 138, 884
Bme18I GGWCC 1 cut(s) 377
BmgBI CACGTC 3 cut(s) 23, 32, 615
BmgT120I GGNCC 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 888
BmrFI CCNGG 2 cut(s) 138, 884
BmsI GCATC 5 cut(s) 59, 261, 327, 349, 552
BpiI GAAGAC 2 cut(s) 395, 444
BpmI CTGGAG 3 cut(s) 137, 312, 854
BpuEI CTTGAG 1 cut(s) 188
Bsa29I ATCGAT 1 cut(s) 335
Bsc4I CCNNNNNNNGG 2 cut(s) 552, 665
Bse1I ACTGG 5 cut(s) 120, 277, 295, 837, 966
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 138, 884
BseCI ATCGAT 1 cut(s) 335
BseLI CCNNNNNNNGG 2 cut(s) 552, 665
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 5 cut(s) 197, 551, 782, 807, 830
BseNI ACTGG 5 cut(s) 120, 277, 295, 837, 966
BseXI GCAGC 1 cut(s) 651
Bsh1236I CGCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 545
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 2 cut(s) 552, 665
BsmAI GTCTC 3 cut(s) 31, 794, 906
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 1 cut(s) 704
Bsp1286I GDGCHC 1 cut(s) 545
Bsp143I GATC 5 cut(s) 11, 320, 796, 848, 886
BspACI CCGC 2 cut(s) 265, 447
BspANI GGCC 1 cut(s) 704
BspCNI CTCAG 5 cut(s) 198, 550, 781, 806, 829
BspDI ATCGAT 1 cut(s) 335
BspFNI CGCG 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 888
BspPI GGATC 4 cut(s) 19, 328, 881, 894
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 5 cut(s) 120, 277, 295, 837, 966
BssMI GATC 5 cut(s) 11, 320, 796, 848, 886
BssSI CACGAG 1 cut(s) 891
Bst2BI CACGAG 1 cut(s) 891
Bst2UI CCWGG 2 cut(s) 138, 884
Bst4CI ACNGT 2 cut(s) 371, 467
Bst6I CTCTTC 2 cut(s) 168, 951
BstC8I GCNNGC 1 cut(s) 123
BstDEI CTNAG 6 cut(s) 206, 537, 768, 793, 816, 859
BstFNI CGCG 1 cut(s) 265
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 3 cut(s) 197, 265, 698
BstKTI GATC 5 cut(s) 14, 323, 799, 851, 889
BstMAI GTCTC 3 cut(s) 31, 794, 906
BstMBI GATC 5 cut(s) 11, 320, 796, 848, 886
BstMWI GCNNNNNNNGC 2 cut(s) 118, 271
BstNI CCWGG 2 cut(s) 138, 884
BstNSI RCATGY 4 cut(s) 357, 423, 529, 625
BstSCI CCNGG 2 cut(s) 136, 882
BstSFI CTRYAG 1 cut(s) 864
BstUI CGCG 1 cut(s) 265
BstV1I GCAGC 1 cut(s) 651
BstV2I GAAGAC 2 cut(s) 395, 444
BstX2I RGATCY 4 cut(s) 11, 320, 796, 886
BstXI CCANNNNNNTGG 1 cut(s) 413
BstYI RGATCY 4 cut(s) 11, 320, 796, 886
Bsu15I ATCGAT 1 cut(s) 335
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 704
BsuTUI ATCGAT 1 cut(s) 335
BtgZI GCGATG 1 cut(s) 82
BtrI CACGTC 3 cut(s) 23, 32, 615
BtsIMutI CAGTG 2 cut(s) 376, 973
Cac8I GCNNGC 1 cut(s) 123
CfoI GCGC 3 cut(s) 197, 265, 698
Cfr13I GGNCC 1 cut(s) 377
ClaI ATCGAT 1 cut(s) 335
Csp6I GTAC 1 cut(s) 417
CviAII CATG 8 cut(s) 85, 354, 420, 497, 526, 550, 622, 646
CviJI RGCY 9 cut(s) 79, 136, 166, 543, 559, 704, 840, 863, 925
CviKI_1 RGCY 9 cut(s) 79, 136, 166, 543, 559, 704, 840, 863, 925
CviQI GTAC 1 cut(s) 417
DdeI CTNAG 6 cut(s) 206, 537, 768, 793, 816, 859
DpnI GATC 5 cut(s) 13, 322, 798, 850, 888
DpnII GATC 5 cut(s) 11, 320, 796, 848, 886
Eam1104I CTCTTC 2 cut(s) 168, 951
EarI CTCTTC 2 cut(s) 168, 951
Ecl136II GAGCTC 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 545
Eco47I GGWCC 1 cut(s) 377
Eco47III AGCGCT 1 cut(s) 196
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 3 cut(s) 59, 175, 615
EcoICRI GAGCTC 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 136, 882
EcoT22I ATGCAT 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 545
FaeI CATG 8 cut(s) 88, 357, 423, 500, 529, 553, 625, 649
FaqI GGGAC 1 cut(s) 500
FatI CATG 8 cut(s) 84, 353, 419, 496, 525, 549, 621, 645
FauI CCCGC 1 cut(s) 440
Fnu4HI GCNGC 1 cut(s) 640
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 1 cut(s) 640
FspBI CTAG 1 cut(s) 846
GlaI GCGC 3 cut(s) 196, 264, 697
GluI GCNGC 1 cut(s) 640
GsuI CTGGAG 3 cut(s) 137, 312, 854
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 1 cut(s) 704
HhaI GCGC 3 cut(s) 197, 265, 698
Hin1II CATG 8 cut(s) 88, 357, 423, 500, 529, 553, 625, 649
Hin6I GCGC 3 cut(s) 195, 263, 696
HinP1I GCGC 3 cut(s) 195, 263, 696
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 6 cut(s) 88, 178, 430, 725, 737, 772
HphI GGTGA 1 cut(s) 843
Hpy188III TCNNGA 4 cut(s) 35, 182, 734, 893
HpyAV CCTTC 4 cut(s) 83, 88, 865, 935
HpyCH4III ACNGT 2 cut(s) 371, 467
HpyCH4IV ACGT 3 cut(s) 22, 31, 614
HpyCH4V TGCA 5 cut(s) 52, 274, 340, 460, 565
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 271
HpyF3I CTNAG 6 cut(s) 206, 537, 768, 793, 816, 859
HpySE526I ACGT 3 cut(s) 22, 31, 614
Hsp92II CATG 8 cut(s) 88, 357, 423, 500, 529, 553, 625, 649
HspAI GCGC 3 cut(s) 195, 263, 696
Kzo9I GATC 5 cut(s) 11, 320, 796, 848, 886
LmnI GCTCC 3 cut(s) 118, 548, 688
Lsp1109I GCAGC 1 cut(s) 651
LweI GCATC 5 cut(s) 59, 261, 327, 349, 552
MaeI CTAG 1 cut(s) 846
MaeII ACGT 3 cut(s) 22, 31, 614
MaeIII GTNAC 1 cut(s) 279
MalI GATC 5 cut(s) 13, 322, 798, 850, 888
MboI GATC 5 cut(s) 11, 320, 796, 848, 886
MboII GAAGA 5 cut(s) 181, 185, 400, 444, 968
MfeI CAATTG 1 cut(s) 44
MflI RGATCY 4 cut(s) 11, 320, 796, 886
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 6 cut(s) 44, 185, 303, 582, 706, 781
MlyI GAGTC 1 cut(s) 424
MmeI TCCRAC 2 cut(s) 570, 580
MnlI CCTC 6 cut(s) 163, 310, 322, 694, 786, 900
Mph1103I ATGCAT 1 cut(s) 342
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 3 cut(s) 212, 483, 764
MspR9I CCNGG 2 cut(s) 138, 884
MunI CAATTG 1 cut(s) 44
MvaI CCWGG 2 cut(s) 138, 884
MvnI CGCG 1 cut(s) 265
MwoI GCNNNNNNNGC 2 cut(s) 118, 271
NdeII GATC 5 cut(s) 11, 320, 796, 848, 886
NlaIII CATG 8 cut(s) 88, 357, 423, 500, 529, 553, 625, 649
NlaIV GGNNCC 1 cut(s) 888
NsiI ATGCAT 1 cut(s) 342
NspI RCATGY 4 cut(s) 357, 423, 529, 625
PciI ACATGT 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 5 cut(s) 88, 178, 725, 737, 772
PfoI TCCNGGA 1 cut(s) 882
PkrI GCNGC 1 cut(s) 641
PleI GAGTC 1 cut(s) 424
PpsI GAGTC 1 cut(s) 424
PscI ACATGT 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 212
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 136, 882
PspGI CCWGG 2 cut(s) 136, 882
PspN4I GGNNCC 1 cut(s) 888
PspPI GGNCC 1 cut(s) 377
PsuI RGATCY 4 cut(s) 11, 320, 796, 886
RsaI GTAC 1 cut(s) 418
RsaNI GTAC 1 cut(s) 417
SacI GAGCTC 1 cut(s) 545
SaqAI TTAA 3 cut(s) 212, 483, 764
SatI GCNGC 1 cut(s) 640
Sau3AI GATC 5 cut(s) 11, 320, 796, 848, 886
Sau96I GGNCC 1 cut(s) 377
SchI GAGTC 1 cut(s) 424
ScrFI CCNGG 2 cut(s) 138, 884
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 5 cut(s) 59, 261, 327, 349, 552
SfcI CTRYAG 1 cut(s) 864
SinI GGWCC 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 6 cut(s) 44, 185, 303, 582, 706, 781
SsiI CCGC 2 cut(s) 265, 447
SspMI CTAG 1 cut(s) 846
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 136, 882
TaaI ACNGT 2 cut(s) 371, 467
TaiI ACGT 3 cut(s) 25, 34, 617
TaqI TCGA 4 cut(s) 63, 287, 335, 778
TasI AATT 6 cut(s) 44, 185, 303, 582, 706, 781
TfiI GAWTC 5 cut(s) 88, 178, 725, 737, 772
Tru1I TTAA 3 cut(s) 212, 483, 764
Tru9I TTAA 3 cut(s) 212, 483, 764
TscAI CASTG 2 cut(s) 376, 973
TseI GCWGC 1 cut(s) 639
TspDTI ATGAA 4 cut(s) 101, 444, 888, 969
TspGWI ACGGA 1 cut(s) 161
TspRI CASTG 2 cut(s) 376, 973
VpaK11BI GGWCC 1 cut(s) 377
VspI ATTAAT 1 cut(s) 212
XapI RAATTY 2 cut(s) 185, 781
XceI RCATGY 4 cut(s) 357, 423, 529, 625
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 1 cut(s) 846
Zsp2I ATGCAT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.