MD00G1088600.v1.1

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003012
Physical Location & Seq
Reverse (-)
18114987 .. 18121993
7007 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1088600.v1.1.491

Sequence Viewer

Length: 972 bp
ATGTCGAGTGGATCCGTTAGACGGGTCTCACGTGAAGATATACAACTGGTGCAAAACCTTATCGAGCGATGTCTCCAGCTTTACATGAACCAGAAGGAAGTGGTAGATACCTTACTGGATCAGGCAAAAATAGAGCCTGGTTTCACAGAACTTGTTTGGCAGAAACTTGAGGAAGAGAATCGGGAATTCTTCAGGGCTTATTATTTAAGACTGATGGTGAAGCACCAAATAATAGAATATAACAACTTGCTTAAGCAACAGGTGGAGTTGATGAGTCAGTTACACTCAAGTAAAGTTGCTTCAATATCTAGTTCCAATGGATCTCAAATTTCATCAATGCACCAAAACTCGACTTGCTACGCACCAGAGCATGTAGGACCAGCGCTGAAGACAGAAAATATACACCACCAAGTTGCTTCCTGCCTACCTAATGCATTTACTAATGGTGGGTCGTCATTGCACACTAGTATGCACAATGCTGTTAAAATGTCTGCTCATACCAATAGGATTGATGTCCAACCAAACATGCTTCCGAACCAGAGCTCCAACATAGGTCTGATGCAAGGAATGAATGGGGGAATTGTCAAATCAGAAGTTGGATATTCAGGCAGTTCGTACATGTTTAGTGCTGATGGAAACATCCTGGAAACGCGGCCCGCTATTGGAGATGCTTCTGTTGCAGCATTCAATAGCGTAGAGTCGAACTCACAGCCCCTAAATGAATCACTGCTTGATGCAGACTGTTCGTCATTTGGTTTTTTAGGTCAGATTCCTCGAAATTTCAGCCTGTCGGATCTGACAGCTGACTTTTCTCAGAGTTCAGACATACTGGAGAGCTATCCTAGATCACCCTTCTTGGCTACAGATAATGACAACTTTCTGGATTCTCGTGATAGAGAACATCAAGGAGACAATAACAGATTGGACACCATTTCAGAAGGTGTAAGTTATGAAGATTTTGGCAGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

324

Amino Acids

35.86

Weight (kDa)

4.94

Isoelectric Point (pI)

58.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 17 - 68 3.4e-26 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 652
AciI CCGC 2 cut(s) 652, 657
AclWI GGATC 5 cut(s) 6, 19, 126, 328, 801
AcsI RAATTY 3 cut(s) 185, 327, 778
AcuI CTGAAG 2 cut(s) 175, 407
AcvI CACGTG 1 cut(s) 32
AfaI GTAC 1 cut(s) 617
AfeI AGCGCT 1 cut(s) 384
AfiI CCNNNNNNNGG 2 cut(s) 21, 662
AflII CTTAAG 1 cut(s) 251
AflIII ACRYGT 1 cut(s) 618
AgsI TTSAA 2 cut(s) 303, 688
AhdI GACNNNNNGTC 1 cut(s) 745
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 2 cut(s) 136, 642
AloI GAACNNNNNNTCC 2 cut(s) 527, 559
AluBI AGCT 4 cut(s) 79, 543, 803, 837
AluI AGCT 4 cut(s) 79, 543, 803, 837
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 3 cut(s) 31, 77, 903
AlwI GGATC 5 cut(s) 6, 19, 126, 328, 801
Aor51HI AGCGCT 1 cut(s) 384
AoxI GGCC 1 cut(s) 653
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 3 cut(s) 185, 327, 778
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 377, 654
AsuHPI GGTGA 2 cut(s) 229, 840
AvaII GGWCC 1 cut(s) 377
BamHI GGATCC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 545
BarI GAAGNNNNNNTAC 2 cut(s) 283, 315
BauI CACGAG 1 cut(s) 888
BbrPI CACGTG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 692
BccI CCATC 2 cut(s) 208, 626
BcgI CGANNNNNNTGC 2 cut(s) 726, 760
BciT130I CCWGG 2 cut(s) 138, 644
BcoDI GTCTC 3 cut(s) 31, 77, 903
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 3 cut(s) 309, 465, 843
BfmI CTRYAG 1 cut(s) 861
BfoI RGCGCY 1 cut(s) 386
BfrI CTTAAG 1 cut(s) 251
BisI GCNGC 2 cut(s) 653, 681
BlsI GCNGC 2 cut(s) 654, 682
Bme1390I CCNGG 2 cut(s) 138, 644
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 745
BmgT120I GGNCC 2 cut(s) 377, 654
BmiI GGNNCC 1 cut(s) 13
BmrFI CCNGG 2 cut(s) 138, 644
BmsI GCATC 3 cut(s) 549, 658, 724
BpiI GAAGAC 1 cut(s) 395
BplI GAGNNNNNCTC 2 cut(s) 689, 721
BpmI CTGGAG 2 cut(s) 59, 851
BpuEI CTTGAG 2 cut(s) 188, 271
BsaAI YACGTR 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 2 cut(s) 21, 662
Bse1I ACTGG 3 cut(s) 51, 120, 834
Bse3DI GCAATG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 138, 644
BseGI GGATG 1 cut(s) 639
BseLI CCNNNNNNNGG 2 cut(s) 21, 662
BseMI GCAATG 1 cut(s) 455
BseMII CTCAG 1 cut(s) 827
BseNI ACTGG 3 cut(s) 51, 120, 834
BseXI GCAGC 1 cut(s) 692
Bsh1236I CGCG 1 cut(s) 652
BshFI GGCC 1 cut(s) 655
BsiHKAI GWGCWC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 21, 662
BsmAI GTCTC 3 cut(s) 31, 77, 903
BsmI GAATGC 1 cut(s) 683
BsnI GGCC 1 cut(s) 655
Bso31I GGTCTC 1 cut(s) 31
Bsp1286I GDGCHC 1 cut(s) 545
Bsp143I GATC 5 cut(s) 11, 118, 320, 793, 845
BspACI CCGC 2 cut(s) 652, 657
BspANI GGCC 1 cut(s) 655
BspCNI CTCAG 1 cut(s) 826
BspFNI CGCG 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 13
BspPI GGATC 5 cut(s) 6, 19, 126, 328, 801
BspTI CTTAAG 1 cut(s) 251
BspTNI GGTCTC 1 cut(s) 31
BsrDI GCAATG 1 cut(s) 455
BsrI ACTGG 3 cut(s) 51, 120, 834
BssMI GATC 5 cut(s) 11, 118, 320, 793, 845
BssSI CACGAG 1 cut(s) 888
Bst2BI CACGAG 1 cut(s) 888
Bst2UI CCWGG 2 cut(s) 138, 644
Bst4CI ACNGT 1 cut(s) 743
Bst6I CTCTTC 1 cut(s) 168
BstAFI CTTAAG 1 cut(s) 251
BstBAI YACGTR 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 657
BstDEI CTNAG 1 cut(s) 813
BstF5I GGATG 1 cut(s) 639
BstFNI CGCG 1 cut(s) 652
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 5 cut(s) 14, 121, 323, 796, 848
BstMAI GTCTC 3 cut(s) 31, 77, 903
BstMBI GATC 5 cut(s) 11, 118, 320, 793, 845
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstNI CCWGG 2 cut(s) 138, 644
BstNSI RCATGY 3 cut(s) 374, 529, 622
BstSCI CCNGG 2 cut(s) 136, 642
BstSFI CTRYAG 1 cut(s) 861
BstUI CGCG 1 cut(s) 652
BstV1I GCAGC 1 cut(s) 692
BstV2I GAAGAC 1 cut(s) 395
BstX2I RGATCY 3 cut(s) 11, 320, 793
BstYI RGATCY 3 cut(s) 11, 320, 793
BsuRI GGCC 1 cut(s) 655
BtgZI GCGATG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 639
BtsI GCAGTG 2 cut(s) 725, 970
BtsIMutI CAGTG 2 cut(s) 725, 970
Cac8I GCNNGC 1 cut(s) 657
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 2 cut(s) 377, 654
Csp6I GTAC 1 cut(s) 616
CviAII CATG 4 cut(s) 85, 371, 526, 619
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 1 cut(s) 813
DpnI GATC 5 cut(s) 13, 120, 322, 795, 847
DpnII GATC 5 cut(s) 11, 118, 320, 793, 845
DriI GACNNNNNGTC 1 cut(s) 745
Eam1104I CTCTTC 1 cut(s) 168
Eam1105I GACNNNNNGTC 1 cut(s) 745
EarI CTCTTC 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 543
Eco24I GRGCYC 1 cut(s) 545
Eco31I GGTCTC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 377
Eco47III AGCGCT 1 cut(s) 384
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 2 cut(s) 175, 407
Eco72I CACGTG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 136, 642
EcoT22I ATGCAT 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 545
FaeI CATG 4 cut(s) 88, 374, 529, 622
FatI CATG 4 cut(s) 84, 370, 525, 618
FauI CCCGC 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 653, 681
FokI GGATG 1 cut(s) 626
FriOI GRGCYC 1 cut(s) 545
Fsp4HI GCNGC 2 cut(s) 653, 681
FspBI CTAG 3 cut(s) 309, 465, 843
GlaI GCGC 1 cut(s) 384
GluI GCNGC 2 cut(s) 653, 681
GsuI CTGGAG 2 cut(s) 59, 851
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 1 cut(s) 655
HhaI GCGC 1 cut(s) 385
Hin1II CATG 4 cut(s) 88, 374, 529, 622
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HinfI GANTC 6 cut(s) 178, 274, 698, 722, 769, 884
HphI GGTGA 2 cut(s) 229, 840
Hpy188I TCNGA 9 cut(s) 534, 558, 592, 768, 793, 798, 816, 823, 937
Hpy188III TCNNGA 3 cut(s) 182, 881, 890
HpyAV CCTTC 3 cut(s) 88, 862, 932
HpyCH4III ACNGT 1 cut(s) 743
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 8 cut(s) 52, 340, 434, 460, 472, 562, 680, 737
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 1 cut(s) 813
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 4 cut(s) 88, 374, 529, 622
HspAI GCGC 1 cut(s) 383
Kzo9I GATC 5 cut(s) 11, 118, 320, 793, 845
LmnI GCTCC 1 cut(s) 548
Lsp1109I GCAGC 1 cut(s) 692
LweI GCATC 3 cut(s) 549, 658, 724
MaeI CTAG 3 cut(s) 309, 465, 843
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 279
MalI GATC 5 cut(s) 13, 120, 322, 795, 847
MboI GATC 5 cut(s) 11, 118, 320, 793, 845
MboII GAAGA 5 cut(s) 47, 181, 185, 400, 965
MflI RGATCY 3 cut(s) 11, 320, 793
MhlI GDGCHC 1 cut(s) 545
MluCI AATT 4 cut(s) 185, 327, 579, 778
MlyI GAGTC 2 cut(s) 283, 707
MmeI TCCRAC 4 cut(s) 541, 570, 577, 771
MnlI CCTC 2 cut(s) 163, 783
Mph1103I ATGCAT 1 cut(s) 436
MseI TTAA 3 cut(s) 206, 252, 483
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 1 cut(s) 803
MspCI CTTAAG 1 cut(s) 251
MspR9I CCNGG 2 cut(s) 138, 644
Mva1269I GAATGC 1 cut(s) 683
MvaI CCWGG 2 cut(s) 138, 644
MvnI CGCG 1 cut(s) 652
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeII GATC 5 cut(s) 11, 118, 320, 793, 845
NlaIII CATG 4 cut(s) 88, 374, 529, 622
NlaIV GGNNCC 1 cut(s) 13
NsiI ATGCAT 1 cut(s) 436
NspI RCATGY 3 cut(s) 374, 529, 622
PciI ACATGT 1 cut(s) 618
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 683
PfeI GAWTC 4 cut(s) 178, 722, 769, 884
PfoI TCCNGGA 1 cut(s) 642
PkrI GCNGC 2 cut(s) 654, 682
PleI GAGTC 2 cut(s) 282, 706
PmaCI CACGTG 1 cut(s) 32
PmlI CACGTG 1 cut(s) 32
PpsI GAGTC 2 cut(s) 282, 706
Ppu21I YACGTR 1 cut(s) 32
PscI ACATGT 1 cut(s) 618
Psp124BI GAGCTC 1 cut(s) 545
Psp6I CCWGG 2 cut(s) 136, 642
PspCI CACGTG 1 cut(s) 32
PspGI CCWGG 2 cut(s) 136, 642
PspN4I GGNNCC 1 cut(s) 13
PspPI GGNCC 2 cut(s) 377, 654
PsuI RGATCY 3 cut(s) 11, 320, 793
PvuII CAGCTG 1 cut(s) 803
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 545
SaqAI TTAA 3 cut(s) 206, 252, 483
SatI GCNGC 2 cut(s) 653, 681
Sau3AI GATC 5 cut(s) 11, 118, 320, 793, 845
Sau96I GGNCC 2 cut(s) 377, 654
SchI GAGTC 2 cut(s) 283, 707
ScrFI CCNGG 2 cut(s) 138, 644
SduI GDGCHC 1 cut(s) 545
SfaNI GCATC 3 cut(s) 549, 658, 724
SfcI CTRYAG 1 cut(s) 861
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 3 cut(s) 167, 251, 286
SmoI CTYRAG 3 cut(s) 167, 251, 286
SpeI ACTAGT 1 cut(s) 464
Sse9I AATT 4 cut(s) 185, 327, 579, 778
SsiI CCGC 2 cut(s) 652, 657
SspMI CTAG 3 cut(s) 309, 465, 843
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 2 cut(s) 136, 642
TaaI ACNGT 1 cut(s) 743
TaiI ACGT 1 cut(s) 34
TaqI TCGA 5 cut(s) 5, 63, 350, 701, 775
TasI AATT 4 cut(s) 185, 327, 579, 778
TauI GCSGC 1 cut(s) 655
TfiI GAWTC 4 cut(s) 178, 722, 769, 884
Tru1I TTAA 3 cut(s) 206, 252, 483
Tru9I TTAA 3 cut(s) 206, 252, 483
TscAI CASTG 2 cut(s) 732, 970
TseI GCWGC 1 cut(s) 680
TspDTI ATGAA 5 cut(s) 101, 321, 584, 735, 966
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 2 cut(s) 732, 970
Vha464I CTTAAG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 3 cut(s) 185, 327, 778
XceI RCATGY 3 cut(s) 374, 529, 622
XspI CTAG 3 cut(s) 309, 465, 843
Zsp2I ATGCAT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.